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951.
ASN1‐encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds
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![点击此处可从《The Plant journal : for cell and molecular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Laure Gaufichon Anne Marmagne Katia Belcram Tadakatsu Yoneyama Yukiko Sakakibara Toshiharu Hase Olivier Grandjean Gilles Clément Sylvie Citerne Stéphanie Boutet‐Mercey Céline Masclaux‐Daubresse Fabien Chardon Fabienne Soulay Xiaole Xu Marion Trassaert Maryam Shakiebaei Amina Najihi Akira Suzuki 《The Plant journal : for cell and molecular biology》2017,91(3):371-393
952.
Maryam Baazm Farideh Jalali Mashayekhi Saeid Babaie Parvindokht Bayat Cordian Beyer Adib Zendedel 《In vitro cellular & developmental biology. Animal》2017,53(8):752-758
Spermatongonial stem cells (SSCs) are unique testis cells that are able to proliferate, differentiate, and transmit genetic information to the next generation. However, the effect of different Sertoli cell types on the expression of specific SSC genes is not yet well understood. In this study, we compare the in vitro effect of adult Sertoli cells, embryonic Sertoli cells, and TM4 (a Sertoli cell line) as feeder layers on the expression of SSC genes. SSCs were isolated from the testis of adult male mice and purified by differential plating. Following enrichment, SSCs were cultivated for 1 and 2 wk in the presence of various feeders. The expression of SSC-specific genes (Mvh, ZBTB, and c-kit) was evaluated by real-time polymerase chain reaction. Our results revealed that expression of the specific SSC genes was significantly higher in the embryonic Sertoli cells after 1 and 2 wk compared to the adult Sertoli cells and the TM4 group. Our finding suggest that co-culturing of SSCs with embryonic Sertoli cells is helpful for in vitro cultivation of SSCs and might improve the self-renewal of these stem cells. 相似文献
953.
Current Status and Perspectives Regarding LNA‐Anti‐miR Oligonucleotides and microRNA miR‐21 Inhibitors as a Potential Therapeutic Option in Treatment of Colorectal Cancer
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![点击此处可从《Journal of cellular biochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
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Somaye Shahraki Maryam Saeidifar Fereshteh Shiri Ameneh Heidari 《Journal of biomolecular structure & dynamics》2017,35(14):3098-3106
In this study, a cytotoxic Pt(IV) complex [Pt(5,5′-dmbpy)Cl4 (5,5′-dmbpy is 5,5′-dimethyl-2,2′-bipyridine) was selected to investigate its affinity to human serum albumin (HSA) by spectroscopy and molecular docking methods. This complex has a bidentate nitrogen donor ligand with four chloride anions attached to a Pt(IV) metal in a distorted octahedral environment. The ?uorescence data showed this complex quench the intrinsic ?uorescence of HSA through a static quenching mechanism. The binding constant (Kb) and the number of binding sites (n) were obtained based on the results of fluorescence measurements. UV–vis, circular dichroism spectroscopy, and three-dimensional fluorescence spectroscopy proved that the Pt(IV) complex could slightly change the secondary structure of protein. Thermodynamic parameters show that the Pt(IV) complex binds to HSA through electrostatic and Vander Waals interactions with one binding site. The molecular docking results confirmed the spectroscopic results and showed that Pt(IV) complex is embedded into subdomain IIA of protein. The aim of this study is to describe the performance of effective anti-cancer drugs when faced with proteins such as HSA. 相似文献
958.
Fatemeh?Mohammadi Ghasem?Solgi Maryam?Tajik Pedram?Ahmadpoor Hasan?Nikoeinejad Behzad?Einollahi Banafsheh?Nazari Mahboob?Lessan-pezeshki Aliakbar?AmirzargarEmail author 《European cytokine network》2017,28(3):93-101
The purpose of this study was to evaluate T-cell immunity markers using serial post-transplantation monitoring of cytokine-producing cells during the first post-transplant months for the prediction of acute rejection and potentially chronic rejection of kidney allograft. We followed 57 kidney allograft recipients for meanly 3 years post-transplantation. Blood samples were collected pre-transplant, 2, 4 and 12 weeks post-transplant. The frequencies of IL-10-, IL-17- and IFN-γ-producing cells were determined in all time-points using ELISPOT assay. The results of ELISpot monitoring and levels of IL-23 and TGF-β were compared between recipients with acute (n = 12) or chronic rejection episodes and patients with stable graft function (n = 45). In all post-transplant time-points, significantly high frequencies of IFN-γ- and IL-17-producing cells and low frequency of IL-10-producing cells were observed in rejection group versus patients with stable graft function (P<0.0001). TheROCcurve analysis for determining the reliability of cytokine-producing cells for the prediction of acute rejection revealed that AUC was 0.046 for IL-10 (P<0.001), 0.927 for IL-17 (P<0.001) and 0.929 for INF-γ-producing cells (P<0.001). Our results indicate that analyzing the frequencies of INF-γ/IL-10/IL-17-producing cells may define a reliable panel for the prediction of acute rejection within the first post-transplant year which could also be applicable for the prediction of chronic rejection episodes. 相似文献
959.
Akram Sadeghi Parisa Koobaz Hossein Azimi Ebrahim Karimi Ali Reza Akbari 《BioControl》2017,62(6):805-819
Phytophthora drechsleri damping-off is one of the most important diseases of cucumber (Cucumis sativus). Salinity is a serious problem for crop production and affects diversity and activity of soil microorganisms. Application of salt-tolerant biocontrol agents may be beneficial in order to protect plants against pathogenic fungi in saline soils. In this study, a total of 717 Streptomyces isolates were isolated from the rhizosphere of cucumber, out of which two isolates showed more than 70% inhibitory effect against P. drechsleri and had cellulase activity in the presence and absence of NaCl. In a greenhouse experiment, two Streptomyces isolates with the highest antagonistic activity, strains C 201 and C 801, reduced seedling damping-off of cucumber caused by P. drechsleri by 77 and 80%, respectively, in artificially infested soils. Strain C 201 increased dry weight of seedlings up to 21% in greenhouse experiments. Phylogenetic analyses of 16S rRNA gene sequence reveals that strains C 201 and C 801 are closely related to S. rimosus and S. monomycini respectively. Increased activity of polyphenol oxidase (PPO) and peroxidase (POX) enzymes in Streptomyces-treated plants proved the biocontrol-induced systemic resistance (ISR) in cucumber plants against P. drechsleri. 相似文献
960.
Reactive oxygen species, antioxidants and signaling in plants 总被引:4,自引:0,他引:4
Several reactive oxygen species (ROS) are continuously produced in plants as byproducts of many metabolic reactions, such
as photosynthesis, photo respiration and respiration, Depending on the nature of the ROS species, some are highly toxic and
rapidly detoxified by various cellular enzymatic and nonenzymatic mechanisms. Oxidative stress occurs when there is a serious
imbalance between the production of ROS and antioxidative defence. ROS participate in signal transduction, but also modify
cellular components and cause damage. ROS is highly reactive molecules and can oxidize all types of cellular components. Various
enzymes involved in ROS-scavenging have been manipulated and over expressed or down regulated. An overview of the literature
is presented in terms of primary antioxidant free radical scavenging and redox signaling in plant cells. Special attention
is given to ROS and ROS-anioxidant interaction as a metabolic interface for different types of signals derived from metabolisms
and from the changing environment. 相似文献