全文获取类型
收费全文 | 1372篇 |
免费 | 91篇 |
国内免费 | 4篇 |
出版年
2024年 | 4篇 |
2023年 | 28篇 |
2022年 | 60篇 |
2021年 | 97篇 |
2020年 | 91篇 |
2019年 | 166篇 |
2018年 | 98篇 |
2017年 | 78篇 |
2016年 | 82篇 |
2015年 | 72篇 |
2014年 | 87篇 |
2013年 | 123篇 |
2012年 | 108篇 |
2011年 | 88篇 |
2010年 | 57篇 |
2009年 | 50篇 |
2008年 | 45篇 |
2007年 | 18篇 |
2006年 | 27篇 |
2005年 | 20篇 |
2004年 | 20篇 |
2003年 | 19篇 |
2002年 | 16篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1988年 | 2篇 |
1985年 | 1篇 |
排序方式: 共有1467条查询结果,搜索用时 15 毫秒
901.
Aliakbar Haddad-Mashadrizeh Ahmad Reza Bahrami Maryam M. Matin Mohammad Amin Edalatmanesh Alireza Zomorodipour Ali Fallah Mossa Gardaneh Naghmeh Ahmadian Kia Naser Sanjarmoosavi 《Cytotherapy》2013,15(8):951-960
Background aimsTherapeutic promises of adult stem cells have been overshadowed by an elicited immune response, low maintenance of implanted cells or concerns regarding their migration to non-target sites. These problems might be lessened by the use of immune privilege cells and tissues for implantation.MethodsIn this study, human adipose-derived mesenchymal stromal cells (hADMSCs) were stably transfected with a vector containing Turbo green fluorescent protein (GFP) and JRed, which allows tracing the cells after transplantation. Labeled hADMSCs were transplanted into the adult rat brain followed by assessment of their survival and migration during 6 months after transplantation.ResultsResults indicate that there were no postsurgical complications, and the animals thrived after transplantation. The lesions of the surgical process were remarkable at the first weeks, and a high number of transplanted cells were accumulated around them. Cell populations declined over time as they partly migrated away from the injection sites; nonetheless, they were detectable at each examination time point. Although the cells could survive and remain at the injection site for up to 6 months, some of them drifted to spleen, which is an indication of their ability to cross the blood-brain barrier.ConclusionsDespite the high survival rate of hADMSCs in the xenogenic condition, which is an ideal criterion in cell therapy, irregular migration tendency must be handled with caution. 相似文献
902.
Roya Khosravi Amin Javidanbardan Maryam Khatami Hooman Kaghazian Seyed Dawood Mousavi Nasab 《Preparative biochemistry & biotechnology》2013,43(7):686-694
AbstractIn mammalian cell culture technology, viral contamination is one of the main challenges; and, so far, various strategies have been taken to remove or inactivate viruses in the cell-line production process. The suitability and feasibility of each method are determined by different factors including effectiveness in target virus inactivation, maintaining recombinant protein stability, easiness—in terms of the process condition, cost-effectiveness, and eco-friendliness. In this research, Taguchi design-of-experiments (DOE) methodology was used to optimize a non-detergent viral inactivation method via considering four factors of temperature, time, pH, and alcohol concentration in an unbiased (orthogonal) fashion with low influence of nuisance factors. Herpes Simplex Virus-1 (HSV1) and Vero cell-line were used as models for enveloped viruses and cell-line, respectively. Examining the cytopathic effects (CPE) in different dilutions showed that pH (4), alcohol (15%), time (120?min), and temperature (25?°C) were the optimal points for viral inactivation. Evaluating the significance of each parameter in the HSV-1 inactivation using Taguchi and ANOVA analyses, the contributions of pH, alcohol, temperature and time were 56.5%, 19.2%, 12%, and 12%, respectively. Examining the impact of the optimal viral treatment condition on the stability of model recombinant protein-recombinant human erythropoietin, no destabilization was detected. 相似文献
903.
Mahnaz Taherianfard Marjan Davazdahemamy Manzarbanoo Shojaeifard Maryam Sharifi 《Journal of physiology and biochemistry》2013,69(1):141-145
Neurosteroids are modulators of neuronal function that may play important role in brain maturation. The aim of the present investigation was to study the effect of prenatal exposure to acute and chronic ethanol on brain progesterone, estradiol, and testosterone concentration on 10th and 15th days following egg incubation. Eggs were exposed to ethanol at 10 % in chronic treatment and 70 % in acute treatment. Progesterone, estradiol, and testosterone were assayed by radioimmunoassay method. It was shown that brain progesterone level was significantly decreased (P?<?0.05) in chronic ethanol group on embryonic day 10, but it was significantly decreased (P?<?0.05) in acute and chronic groups on embryonic day 15. Brain estradiol level was significantly increased (P?<?0.05) in chronic ethanol group on embryonic day 10, and it was decreased (P?<?0.05) in acute and chronic groups of ethanol on embryonic day 15. Brain testosterone was significantly increased (P?<?0.05) in acute and chronic ethanol-exposed groups on embryonic days 10 and 15. Our observations suggest that ethanol may modulate neurosteroid synthesis in the brain. 相似文献
904.
905.
Li Alemo Munters Maryam Dastmalchi Abram Katz Mona Esbj?rnsson Ingela Loell Balsam Hanna Maria Lidén H?kan Westerblad Ingrid E Lundberg Helene Alexanderson 《Arthritis research & therapy》2013,15(4):R83
Introduction
This randomized, controlled study on patients with polymyositis or dermatomyositis was based on three hypotheses: patients display impaired endurance due to reduced aerobic capacity and muscle weakness, endurance training improves their exercise performance by increasing the aerobic capacity, and endurance training has general beneficial effects on their health status.Methods
In the first part of this study, we compared 23 patients with polymyositis or dermatomyositis with 12 age- and gender-matched healthy controls. A subgroup of patients were randomized to perform a 12-week endurance training program (exercise group, n = 9) or to a non-exercising control group (n = 6). We measured maximal oxygen uptake (VO2 max) and the associated power output during a progressive cycling test. Endurance was assessed as the cycling time to exhaustion at 65% of VO2 max. Lactate levels in the vastus lateralis muscle were measured with microdialysis. Mitochondrial function was assessed by measuring citrate synthase (CS) and β-hydroxyacyl-CoA dehydrogenase (β-HAD) activities in muscle biopsies. Clinical improvement was assessed according to the International Myositis Assessment and Clinical Studies Group (IMACS) improvement criteria. All assessors were blinded to the type of intervention (that is, training or control).Results
Exercise performance and aerobic capacity were lower in patients than in healthy controls, whereas lactate levels at exhaustion were similar. Patients in the exercise group increased their cycling time, aerobic capacity and CS and β-HAD activities, whereas lactate levels at exhaustion decreased. Six of nine patients in the exercise group met the IMACS improvement criteria. Patients in the control group did not show any consistent changes during the 12-week study.Conclusions
Polymyositis and dermatomyositis patients have impaired endurance, which could be improved by 12 weeks of endurance training. The clinical improvement corresponds to increases in aerobic capacity and muscle mitochondrial enzyme activities. The results emphasize the importance of endurance exercise in addition to immunosuppressive treatment of patients with polymyositis or dermatomyositis.Trial registration
ClinicalTrials.gov: NCT01184625相似文献906.
Response surface optimized peroxyoxalate chemiluminescence of octahydro‐Schiff base derivative as new luminophor and study of the quenching effect of some cations,amino acids and cholesterol
下载免费PDF全文
![点击此处可从《Luminescence》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ali Yeganeh Faal Bahare Jamalyan Maryam Bordbar Tavakol Heidary Shayeste Masoud Salavati‐Niasari 《Luminescence》2014,29(8):1074-1081
We report the first detailed study of the characteristics of an octahydro‐Schiff base derivative as a new luminophor in the peroxyoxalate chemiluminescence (POCL) system. The effect of reagents on this new POCL system was investigated. In addition, the response surface methodology was used for evaluating the relative significance of variables in this POCL system, establishing models and determining optimal conditions. The quenching effect of some cations and compounds such as Cu2+, Fe3+, Hg2+, imidazole, histidine and cholesterol on an optimized POCL reaction were studied. The dynamic ranges were up to approximaterly 100 and 175 × 10?6 M for Cu2+ and cholesterol respectively. The detection limits were 3.3 × 10?6 m and 2.58 × 10?6 m for Cu2+ and histidine, respectively. In all cases the relative standard deviations were 4–5% (n = 4). Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
907.
Margherita Bertuzzi Markus Schrettl Laura Alcazar-Fuoli Timothy C. Cairns Alberto Mu?oz Louise A. Walker Susanne Herbst Maryam Safari Angela M. Cheverton Dan Chen Hong Liu Shinobu Saijo Natalie D. Fedorova Darius Armstrong-James Carol A. Munro Nick D. Read Scott G. Filler Eduardo A. Espeso William C. Nierman Hubertus Haas Elaine M. Bignell 《PLoS pathogens》2014,10(10)
908.
Abdolhamed Kian Sayeh Parkhideh Haniyeh Ghaffari Nazari Maryam Nikoonezhad Arsalan Jalili Shaghayegh Shahsavan Abbas Hajifathali 《Reports of Biochemistry & Molecular Biology》2021,10(1):76
Background:Parathyroid hormone (PTH) is a calcium homeostasis regulator and can affect bone marrow niche. PTH leads to the bone marrow stem cell niche expansion as well as the induction of stem cell mobilization from the bone marrow into peripheral blood. In this study, we evaluated the association between pre- transplantation serum PTH levels and the number of circulating CD34+ cells along with the platelets/white blood cells (Plt/WBC) engraftment in patients who underwent autologous Hematopoietic Stem Cell Transplantation.Methods:Subjects for the study were 100 patients who received autologous hematopoietic stem cell transplantation (auto-HSCT), retrospectively. Serum levels of PTH, calcium, phosphorus, and alkaline phosphatase were measured before mobilization. Their impacts were measured on the number of mobilized CD34+ hematopoietic stem cells, and Plt/WBC engraftment.Results:High levels of serum PTH (> 63.10 pg/mL) was significantly associated with higher number of CD34+ cells in peripheral blood after granulocyte- colony stimulating factor (G-CSF)-induced mobilization (p= 0.079*). Serum calcium at low levels were associated with higher number of circulating CD34+ cells post mobilization. Pre- transplantation serum levels of phosphorus and alkaline phosphatase on CD34+ numbers were not statistically significant. Serum Plt/WBC engraftment was not improved in presence of high levels of serum PTH.Conclusion:We suggested that serum PTH levels before transplantation could be influential in raising the number of circulating CD34+ hematopoietic stem cell after mobilization.Key Words: Auto-HSCT, CD34+ Cell, Pre- transplant PTH 相似文献
909.
Mahmoudian Reihaneh Alsadat Gharaie Maryam Lotfi Abbaszadegan Mohammad Reza Alasti Ali Forghanifard Mohammad Mahdi Mansouri Atena Gholamin Mehran 《Molecular and cellular biochemistry》2021,476(6):2465-2478
Molecular and Cellular Biochemistry - Matrix metalloproteinases (MMPs) play key roles in epithelial-mesenchymal transition (EMT) for the development of cancer cell invasion and metastasis. MMP-13... 相似文献
910.