全文获取类型
收费全文 | 1364篇 |
免费 | 91篇 |
国内免费 | 4篇 |
出版年
2024年 | 4篇 |
2023年 | 25篇 |
2022年 | 56篇 |
2021年 | 97篇 |
2020年 | 91篇 |
2019年 | 166篇 |
2018年 | 98篇 |
2017年 | 78篇 |
2016年 | 82篇 |
2015年 | 72篇 |
2014年 | 87篇 |
2013年 | 123篇 |
2012年 | 108篇 |
2011年 | 88篇 |
2010年 | 57篇 |
2009年 | 50篇 |
2008年 | 45篇 |
2007年 | 18篇 |
2006年 | 26篇 |
2005年 | 20篇 |
2004年 | 20篇 |
2003年 | 19篇 |
2002年 | 16篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1988年 | 2篇 |
1985年 | 1篇 |
排序方式: 共有1459条查询结果,搜索用时 31 毫秒
121.
Aghamohamadi Elham Asri † Nastaran Odak Aylin Rostami-Nejad Mohammad Chaleshi Vahid Hajinabi Yasaman Eslami Maryam Mohammadian Haftcheshmeh Saeed Gholam-Mostafaei Fahimeh Sadat Asadzadeh-Aghdaei Hamid Masotti Andrea Zali Mohammad Reza 《Molecular biology reports》2022,49(7):6085-6091
Molecular Biology Reports - Celiac disease (CeD) and inflammatory bowel disease (IBD) are accompanied by impaired immune responses. To study the immune regulation of these diseases, we evaluated... 相似文献
122.
Jenabi Maryam Khodarahmi Parvin Tafvizi Farzaneh Bostanabad Saeed Zaker 《Molecular biology reports》2022,49(9):8413-8427
Molecular Biology Reports - The present study aimed to evaluate the expression of the chemokine CXCL8 in both mRNA and protein levels in the serum, follicular fluid (FF), and cumulus cells (CCs)... 相似文献
123.
Gharbi Dorra Mobayed Hassan M. Ali Ramzy Mohammed Tuffaha Amjad Dason Blessing Reena Ibrahim Tayseer Adeli Mehdi Sattar Hisham A. Trigo Maria del Mar Al-Nesf Maryam Ali 《Aerobiologia》2022,38(3):329-342
Aerobiologia - Daily monitoring of airborne fungal spores was carried out for the first time in Al Khor city, Qatar, using a Hirst type 7-day recording volumetric spore trap, from May 2017 to May... 相似文献
124.
Mohammadi Salman Shafiee Maryam Faraji Seyed Nooreddin Rezaeian Mohsen Ghaffarian-Bahraman Ali 《Biometals》2022,35(4):711-728
BioMetals - Breast milk is a complete food for the development of the newborn, but it can also be an important route for environmental pollutants transmission to the infants. This study was aimed... 相似文献
125.
126.
Muhammad Khan Amara Maryam Javed Iqbal Qazi Tonghui Ma 《International journal of biological sciences》2015,11(9):1100-1112
Cancer is the second leading cause of deaths worldwide. Despite concerted efforts to improve the current therapies, the prognosis of cancer remains dismal. Highly selective or specific blocking of only one of the signaling pathways has been associated with limited or sporadic responses. Using targeted agents to inhibit multiple signaling pathways has emerged as a new paradigm for anticancer treatment. Icariside II, a flavonol glycoside, is one of the major components of Traditional Chinese Medicine Herba epimedii and possesses multiple biological and pharmacological properties including anti-inflammatory, anti-osteoporosis, anti-oxidant, anti-aging, and anticancer activities. Recently, the anticancer activity of Icariside II has been extensively investigated. Here, in this review, our aim is to give our perspective on the current status of Icariside II, and discuss its natural sources, anticancer activity, molecular targets and the mechanisms of action with specific emphasis on apoptosis pathways which may help the further design and conduct of preclinical and clinical trials.Icariside II has been found to induce apoptosis in various human cancer cell lines of different origin by targeting multiple signaling pathways including STAT3, PI3K/AKT, MAPK/ERK, COX-2/PGE2 and β-Catenin which are frequently deregulated in cancers, suggesting that this collective activity rather than just a single effect may play an important role in developing Icariside II into a potential lead compound for anticancer therapy. This review suggests that Icariside II provides a novel opportunity for treatment of cancers, but additional investigations and clinical trials are still required to fully understand the mechanism of therapeutic effects to further validate it in anti-tumor therapy. 相似文献
127.
Milani P Gholamirad M Traas J Arnéodo A Boudaoud A Argoul F Hamant O 《The Plant journal : for cell and molecular biology》2011,67(6):1116-1123
Whereas the morphogenesis of developing organisms is relatively well understood at the molecular level, the contribution of the mechanical properties of the cells to shape changes remains largely unknown, mainly because of the lack of quantified biophysical parameters at cellular or subcellular resolution. Here we designed an atomic force microscopy approach to investigate the elastic modulus of the outer cell wall in living shoot apical meristems (SAMs). SAMs are highly organized structures that contain the plant stem cells, and generate all of the aerial organs of the plant. Building on modeling and experimental data, we designed a protocol that is able to measure very local properties, i.e. within 40-100 nm deep into the wall of living meristematic cells. We identified three levels of complexity at the meristem surface, with significant heterogeneity in stiffness at regional, cellular and even subcellular levels. Strikingly, we found that the outer cell wall was much stiffer at the tip of the meristem (5 ± 2 MPa on average), covering the stem cell pool, than on the flanks of the meristem (1.5 ± 0.7 MPa on average). Altogether, these results demonstrate the existence of a multiscale spatialization of the mechanical properties of the meristem surface, in addition to the previously established molecular and cytological zonation of the SAM, correlating with regional growth rate distribution. 相似文献
128.
Evidence for evolutionary constraints in <Emphasis Type="Italic">Drosophila</Emphasis> metal biology
Mutations in single Drosophila melanogaster genes can alter total body metal accumulation. We therefore asked whether evolutionary constraints maintain biologically
abundant metal ions (iron, copper, manganese and zinc) to similar concentrations in different species of Drosophilidae, or
whether metal homeostasis is a highly adaptable trait as shown previously for triglyceride and glycogen storage. To avoid
dietary influences, only species able to grow and reproduce on a standard laboratory medium were selected for analysis. Flame
atomic absorption spectrometry was used to determine metal content in 5-days-old adult flies. Overall, the data suggest that
the metallome of the nine species tested is well conserved. Meaningful average values for the Drosophilidae family are presented.
Few statistically significant differences were noted for copper, manganese and zinc between species. In contrast, Drosophila erecta and Drosophila virilis showed a 50% increase above average and a 30% decrease below average in iron concentrations, respectively. The changes in
total body iron content correlated with altered iron storage in intestinal ferritin stores of these species. Hence, the variability
in iron content could be accounted for by a corresponding adaptation in iron storage regulation. We suggest that the relative
expression of the multitude of metalloenzymes and other metal-binding proteins remains overall similar between species and
likely determines relative metal abundances in the organism. The availability of a complete and annotated genome sequence
of different Drosophila species presents opportunities to study the evolution of metal homeostasis in closely related organisms that have evolved
separately for millions or dozens of million years. 相似文献
129.
Habib Bokhari Maryam Anwar Hasan Bilal Mirza Patrick Martin Gillevet 《Bioinformation》2011,6(8):293-296
Acquisition of new genetic material through horizontal gene transfer has been shown to be an important feature in the evolution of many pathogenic bacteria.
Changes in the genetic repertoire, occurring through gene acquisition and deletion, are the major events underlying the emergence and evolution of bacterial
pathogens. However, horizontal gene transfer across the domains i.e. archaea and bacteria is not so common. In this context, we explore events of horizontal gene
transfer between archaea and bacteria. In order to determine whether the acquisition of archaeal genes by lateral gene transfer is an important feature in the
evolutionary history of the pathogenic bacteria, we have developed a scheme of stepwise eliminations that identifies archaeal-like genes in various bacterial
genomes. We report the presence of 9 genes of archaeal origin in the genomes of various bacteria, a subset of which is also unique to the pathogenic members and
are not found in respective non-pathogenic counterparts. We believe that these genes, having been retained in the respective genomes through selective advantage,
have key functions in the organism’s biology and may play a role in pathogenesis. 相似文献
130.
Rezazadeh M Yamini Y Seidi S 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2011,879(15-16):1143-1148
Nalmefene and naltrexone are used to block the effects of narcotics and alcohol. In the present work, for the first time a microextraction technique was presented to reduce matrix interferences and improve detection limits of the drugs in urine and plasma samples. Electromembrane extraction (EME) followed by high performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection was optimized and validated for quantification of nalmefene and naltrexone from biological fluids. The membrane consists 85% of 2-nitrophenyl octyl ether (NPOE) and 15% di-(2-ethylhexyl) phosphate (DEHP) immobilized in the pores of a hollow fiber. A 100 V electrical field was applied to make the analytes migrate from sample solution with pH 2.0, through the supported liquid membrane (SLM) into an acidic acceptor solution with pH 1.0 which was located inside the lumen of hollow fiber. Extraction recoveries in the range of 54% and 75% were obtained in different biological matrices which resulted in preconcentration factors in the range of 109-149 and satisfactory repeatability (2.0相似文献