全文获取类型
收费全文 | 148篇 |
免费 | 12篇 |
出版年
2022年 | 1篇 |
2021年 | 4篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2016年 | 2篇 |
2015年 | 2篇 |
2014年 | 1篇 |
2013年 | 5篇 |
2012年 | 11篇 |
2011年 | 14篇 |
2010年 | 5篇 |
2009年 | 3篇 |
2008年 | 5篇 |
2007年 | 4篇 |
2006年 | 5篇 |
2005年 | 2篇 |
2004年 | 6篇 |
2003年 | 3篇 |
2002年 | 8篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 4篇 |
1973年 | 3篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有160条查询结果,搜索用时 459 毫秒
151.
152.
E. Maruta 《Trees - Structure and Function》1996,11(2):119-126
A krummholz mat of larch, Larix leptolepis, is the predominant growth form near the upper limit (near 2500 m) of the timberline ecotone on the south slope of Mt. Fuji,
Japan. On the south-eastern slope, the tree line is lowered to 1600 m because of the last volcanic eruption. The extent and
causes of winter desiccation were compared in timberline larch between 2500 m and 1600 m elevation over two winters. Bark
abrasion due to wind-blown fine volcanic gravels caused a decrease in bark resistance to water loss and resulted in severe
desiccation damage to current-year shoots of krummholz larches in the winter of 1986 – 87 at 2500 m, whereas abraded shoots
at 1600 m maintained high water content during both winters. In the winter of 1985 – 86, shoots of krummholz larches at 2500
m did not experience bark abrasion and high water contents were maintained. Experimental abrasion of shoot surfaces resulted
in similar results at each elevation. Thus, in timberline larch at 2500 m, abrasion by wind-blown fine volcanic gravels is
the primary factor causing winter desiccation damage and krummholz formation. Based on field experiments, the estimated amount
of water movement to non-abraded shoots was the same for the two elevations. At 2500 m, water movement to abraded shoots was
less than to non-abraded shoots, but the reverse situation was noted at 1600 m. Water supply to abraded shoots at 2500 m was
limited and insufficient to compensate for water loss. A cause of limited water supply at 2500 m may be xylem embolism.
Received: 13 January 1995 / Accepted: 31 January 1996 相似文献
153.
Genetica - Tanytarsini is a large tribe of Chironomidae with at least 11 recorded marine species grouped in three genera. In this study, we performed a phylogenic analysis using molecular data from... 相似文献
154.
Ca2+-dependent actin-binding phosphoprotein in Physarum polycephalum. Subunit b is a DNase I-binding and F-actin capping protein 总被引:4,自引:0,他引:4
Physarum contains at least two distinct DNase I-binding proteins, i.e. actin and Cap 42 (a + b). The latter, a tight (1:1) complex of Cap 42 (a) and Cap 42 (b) (Maruta, H., Isenberg. G., Schreckenbach, T., Hallmann, R., Risse, G., Schibayama, T., and Hesse, J. (1983) J. Biol. Chem. 258, 10144-10150), is a Ca2+-dependent F-actin capping protein. DNase I binds to Cap 42 (b) but not to Cap 42 (a). Consequently, DNase I-agarose was used for an affinity-purification of Cap 42 (a + b), after its separation from actin by DEAE-cellulose chromatography. Cap 42 (a + b) was dissociated into its subunits when released from DNase I-agarose by 8.8 M formamide. The two subunits were subsequently separated from each other on hydroxylapatite. Both Cap 42 (a) and Cap 42 (b) were Ca2+-dependent F-actin capping proteins that cap the fast growing end of actin filaments and block actin polymerization at this end. Like Cap 42 (a + b), Cap 42 (b) required Ca2+ for its capping activity only when phosphorylated. The phosphorylation of Cap 42 (b) was completely blocked by DNase I or a tertiary complex of Cap 42 (a), actin, and Ca2+. Cap 42 (b) is not identical with native (= polymerizable) actin because (i) Cap 42 (b) was unable to form filaments, (ii) the Cap 42 (b) kinase did not phosphorylate native actin, and (iii) fragmin formed a tight (1:1) complex with native actin but not with Cap 42 (b). Although it is unlikely that Cap 42 (b) is simply a denatured form of actin that has lost its polymerizability during the preparation, it still remains to be clarified whether Cap 42 (b) is a nonpolmerizable actin variant derived from a distinct actin gene or a post-translationally modified form of polymerizable actin. 相似文献
155.
156.
157.
Natsumi Maruta Yuri Trusov Daisuke Urano David Chakravorty Sarah M Assmann Alan M Jones Jose R Botella 《Plant physiology》2021,186(2):1240
The extra-large guanosine-5′-triphosphate (GTP)-binding protein 2, XLG2, is an unconventional Gα subunit of the Arabidopsis (Arabidopsis thaliana) heterotrimeric GTP-binding protein complex with a major role in plant defense. In vitro biochemical analyses and molecular dynamic simulations show that affinity of XLG2 for GTP is two orders of magnitude lower than that of the conventional Gα, AtGPA1. Here we tested the physiological relevance of GTP binding by XLG2. We generated an XLG2(T476N) variant with abolished GTP binding, as confirmed by in vitro GTPγS binding assay. Yeast three-hybrid, bimolecular fluorescence complementation, and split firefly-luciferase complementation assays revealed that the nucleotide-depleted XLG2(T476N) retained wild-type XLG2-like interactions with the Gβγ dimer and defense-related receptor-like kinases. Both wild-type and nucleotide-depleted XLG2(T476N) restored the defense responses against Fusarium oxysporum and Pseudomonas syringae compromised in the xlg2 xlg3 double mutant. Additionally, XLG2(T476N) was fully functional restoring stomatal density, root growth, and sensitivity to NaCl, but failed to complement impaired germination and vernalization-induced flowering. We conclude that XLG2 is able to function in a GTP-independent manner and discuss its possible mechanisms of action.Arabidopsis extra-large GTP-binding proteins have nucleotide-independent functions. 相似文献
158.
159.
160.
Hiroshi Miyamoto Siroj Jitsurong Ryoko Shiota Koji Maruta Shin-Ichi Yoshida Eiko Yabuuchi 《Microbiology and immunology》1997,41(3):197-202
To determine the infection source of a sporadic Legionella pneumonia case associated with a hot spring bath, we used five molecular methods, including repetitive element polymerase chain reaction (rep-PCR), arbitrarily primed PCR (AP-PCR), ribotyping, restriction endonuclease analysis (REA), and macrorestriction endonuclease analysis (MREA) by pulsed-field gel electrophoresis. L. pneumophila serogroup (SG) 3 strain EY 3702, isolated from an intratracheal specimen of a 71-year-old Japanese female who developed pneumonia after nearly drowning in a hot spring spa bath, produced rep-PCR and AP-PCR fingerprints identical to those of L. pneumophila SG 3 strains EY 3768 and EY 3769 isolated from the bath water. Four epidemiologically unrelated L. pneumophila SG 3 strains showed different rep-PCR or AP-PCR fingerprints from those of the three EY strains (EY 3702, 3768, and 3769). The three EY strains were also genotypically indistinguishable by ribotyping with EcoRI and PstI, by REA with EcoBI or HindIII, and by MREA with NotI. Based on these results, we identified the bath water of the hot spring spa as the source of infection of this patient, even though the viable number of the organisms in the bath water was low (3 CFU/100 ml) when determined 27 days after her nearly drowning. 相似文献