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961.
Martin R. Ciancio Mariela C. Castro Fernando C. Galliari Alfredo A. Carlini Robert J. Asher 《Journal of Mammalian Evolution》2012,19(1):1-8
Late eruption of the permanent dentition was recently proposed as a shared anatomical feature of endemic African mammals (Afrotheria),
with anecdotal reports indicating that it is also present in dasypodids (armadillos). In order to clarify this question, and
address the possiblity that late eruption is shared by afrotherians and dasypodids, we quantified the eruption of permanent
teeth in Dasypus, focusing on growth series of D. hybridus and D. novemcinctus. This genus is the only known xenarthran that retains two functional generations of teeth. Its adult dentition typically
consists of eight upper and eight lower ever-growing (or euhypsodont) molariforms, with no premaxillary teeth. All but the
posterior-most tooth are replaced, consistent with the identification of a single molar locus in each series. Comparison of
dental replacement and skull metrics reveals that most specimens reach adult size with none or few erupted permanent teeth.
This pattern of growth occurring prior to the full eruption of the dentition is similar to that observed in most afrotherians.
The condition observed in Dasypus and many afrotherians differs from that of most other mammals, in which the permanent dentition erupts during (not after)
growth, and is complete at or near the attainment of sexual maturity and adult body size. The suture closure sequence of basicranial
and postcranial epiphyses does not correlate well with dental eruption. The basal phylogenetic position of the taxon within
dasypodids suggests that diphyodonty and late dental replacement represent the condition of early xenarthrans. Additionally,
the inferred reduction in the number of molars to a single locus and the multiplication of premolars represent rare features
for any living mammal, but may represent apomorphic characters for Dasypus. 相似文献
962.
963.
964.
We propose a flexible and identifiable version of the 2-groups model, motivated by hierarchical Bayes considerations, that features an empirical null and a semiparametric mixture model for the nonnull cases. We use a computationally efficient predictive recursion (PR) marginal likelihood procedure to estimate the model parameters, even the nonparametric mixing distribution. This leads to a nonparametric empirical Bayes testing procedure, which we call PRtest, based on thresholding the estimated local false discovery rates. Simulations and real data examples demonstrate that, compared to existing approaches, PRtest's careful handling of the nonnull density can give a much better fit in the tails of the mixture distribution which, in turn, can lead to more realistic conclusions. 相似文献
965.
966.
Per Putkonen Charlotta Nilsson Kerstin Hild Reinhold Benthin Martin Cranage Anne Marie Aubertin Gunnel Biberfeld 《Journal of medical primatology》1993,22(2-3):100-103
Several groups have reported protection against experimental SIV infection in macaques immunized with a whole inactivated virus vaccine. The aim of the current study was to investigate whether five macaques vaccinated with whole inactivated SIV and previously shown to be protected against challenge with two divergent strains of SIV grown on human cells could resist challenge with a subsequent homologous SIV grown on macaque cells. We show here that this same vaccine did not protect when the challenge virus was grown on primary cells of monkey origin. 相似文献
967.
Measurement and verification of rates of sediment phosphorus release for a hypereutrophic urban lake 总被引:1,自引:0,他引:1
The contribution of sediment release to the phosphorus budget of hypereutrophic Onondaga Lake was determined through laboratory measurements made on intact cores. Rates ranged from 9–21 mg P m–2 d–1 with a mean of 13 mg P m–2 d–1, values similar to those observed in other lakes of comparable trophic state. There was no statistically significant trend in rates in time (July versus September) or in space (location along the major N/S axis of the lake). Rates of sediment phosphorus release measured in the laboratory compared favorably with the observed rate of soluble reactive phosphorus accumulation in the lake's hypolimnion. The sediments are the second largest source of phosphorus for Onondaga Lake, contributing 24% of the overall phosphorus load to the system. 相似文献
968.
Entomopathogenic nematodes of the Heterorhabditidae and Steinernematidae appear to be capable of long-distance dispersal and local migration. Their transmission strategies include both highly active seek-and-destroy behaviours and ambusher strategies, and they may be sensitive to sex-related factors in their own populations. Their host-finding abilities are poorly understood, despite the fact that these abilities are fundamental to their success as biocontrol agents in soil. Like the vast numbers of exotic hymenopterans and other natural enemies that have been released for biological control over the past century, they may be used in their ecologically competent wild-type form. On the other hand, because they are applied inundatively, they may be tailored, by breeding or transformation, to their intended purpose and to ecological incompetence, improving both their efficacy and their ecological safety. 相似文献
969.
Margaret Smallwood Edwin A. Yates William G. T. Willats Helen Martin J. Paul Knox 《Planta》1996,198(3):452-459
Arabinogalactan-proteins (AGPs) occurring in suspension-cultured rice (Oryza saliva L.) cells, their conditioned medium and at the rice root apex were investigated using monoclonal antibodies and the AGP-binding -glucosyl Yariv reagent ( GlcY). A monoclonal antibody, LM2, was generated that recognized an acidic carbohydrate epitope common to two soluble AGPs occurring in the conditioned medium of proliferating rice cells, membrane-associated AGPs (rmAGP) in the cultured cells and two AGPs at the rice root apex. In addition, LM2 recognized AGPs secreted by suspensioncultured carrot (Daucus carota L.) cells. The two AGPs of the rice culture medium, srAGP1 and srAGP2, were discriminated by their mobilities during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, reaction with GlcY, the presence of arabinogalactan epitopes and anion-exchange chromatography. The association of rmAGP with the plasma membrane was investigated by Triton-X-114/aqueous partitioning of both microsomal and plasma-membrane preparations and rmAGP was found to partition into the detergent phase, indicating that AGPs are hydrophobic plasma-membrane proteins in rice. This was in contrast to plasma-membrane AGPs of suspension-cultured carrot cells that partitioned into the aqueous phase. At the rice root apex most of the AGP was associated with the microsomal fraction and also partitioned into the detergent phase, although a distinct highmolecular-mass AGP entered the aqueous phase.Abbreviations AGP
arabinogalactan-protein
- GlcY
-glucosyl Yariv reagent
- ELISA
enzyme-linked immunosorbent assay
We gratefully acknowledge support from the Leverhulme Trust, the UK Biotechnology and Biological Sciences Research Council and the Royal Society. 相似文献
970.
Walid N. Abouhamad Dennis Bray Martin Schuster Kristin C. Boesch Ruth E. Silversmith Robert B. Bourret 《Journal of bacteriology》1998,180(15):3757-3764
Escherichia coli responds to its environment by means of a network of intracellular reactions which process signals from membrane-bound receptors and relay them to the flagellar motors. Although characterization of the reactions in the chemotaxis signaling pathway is sufficiently complete to construct computer simulations that predict the phenotypes of mutant strains with a high degree of accuracy, two previous experimental investigations of the activity remaining upon genetic deletion of multiple signaling components yielded several contradictory results (M. P. Conley, A. J. Wolfe, D. F. Blair, and H. C. Berg, J. Bacteriol. 171:5190–5193, 1989; J. D. Liu and J. S. Parkinson, Proc. Natl. Acad. Sci. USA 86:8703–8707, 1989). For example, “building up” the pathway by adding back CheA and CheY to a gutted strain lacking chemotaxis genes resulted in counterclockwise flagellar rotation whereas “breaking down” the pathway by deleting chemotaxis genes except cheA and cheY resulted in alternating episodes of clockwise and counterclockwise flagellar rotation. Our computer simulation predicts that trace amounts of CheZ expressed in the gutted strain could account for this difference. We tested this explanation experimentally by constructing a mutant containing a new deletion of the che genes that cannot express CheZ and verified that the behavior of strains built up from the new deletion does in fact conform to both the phenotypes observed for breakdown strains and computer-generated predictions. Our findings consolidate the present view of the chemotaxis signaling pathway and highlight the utility of molecularly based computer models in the analysis of complex biochemical networks. 相似文献