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101.
While long-term fixation and storage of specimens is common and useful for many research projects, it is particularly important for space flight investigations where samples may not be returned to Earth for several months (International Space Station) or years (manned mission to Mars). We examined two critical challenges of space flight experimentation: the effect of long-term fixation on the quality of mouse bone preservation and the preservation of antigens and enzymes for both histochemical and immunohistochemical analyses, and how the animal/sample processing affects the preservation. We show that long-term fixation minimally affects standard histological staining, but that enzyme histochemistry and immunolabeling are greatly compromised. Further, we demonstrate that whole animal preservation is not as suitable as whole leg or stripped leg preservation for long-term fixation and all histological analyses. Overall, we recommend whole leg processing for long-term storage of bone specimens in fixative prior to embedding in plastic for histological examination. 相似文献
102.
Marti J Anderson Russell B Millar 《Journal of experimental marine biology and ecology》2004,305(2):191-221
Reef-associated fishes can respond to changes in habitat structure and the nature of their response can change with different spatial scales of observation. A structured hierarchical mensurative sampling design was used to sample temperate reef fish assemblages in northeastern New Zealand at several spatial scales over 2 years. The three spatial scales examined were tens of meters (transects), hundreds to thousands of meters (sites) and hundreds of kilometers (locations). We tested the hypothesis that fish assemblages differed between kelp forest habitat (relatively dense stands of the kelp, Ecklonia radiata (C. Agardh) J. Agardh, median depth=13.5 m) and barrens habitat (rocky reef dominated by turfing and encrusting red algae and the grazing urchin, Evechinus chloroticus (Valenciennes), median depth=6.7 m). Recently developed multivariate techniques were used to test for and quantify multivariate variation at different spatial scales. There were significant effects of habitat on the spatial distribution of fish assemblages, characterised by greater abundances or frequencies of Parika scaber, Chromis dispilus, Trachurus novaezelandiae, Nemadactylus douglasii, Bodianus unimaculatus, Odax pullus and Pseudolabrus miles in kelp forest habitat, and greater abundances or frequencies of Notolabrus celidotus, Notolabrus fucicola, Girella tricuspidata, Coris sandageri, Chironemus marmoratus, Parma alboscapularis, Scorpis violaceus and Kyphosus sydneyanus in barrens habitat. Some of the more common species, including Upeneichthys lineatus, Scorpis lineolatus and Cheilodactylus spectabilis showed no strong consistent effects of these two differing habitats on their distributions. There was, however, a significant Habitat×Locations interaction: effects of habitat did not occur at all locations. Variability was highest at the scale of individual transects and variability from site to site and from location to location was comparable. Spatial variation was large compared to inter-annual variation, which was minimal, and spatial patterns were consistent in the 2 years examined. Further experiments, including manipulations, are required to understand what mechanisms and processes might be driving these patterns. This study, coupled with results from previous studies, suggests that there may be a dynamic inter-play between effects of habitat on fish and effects of fish on biogenic habitat, such as kelp forests. 相似文献
103.
Since the discovery of soluble guanylate cyclase (sGC) as the mammalian receptor for nitric oxide (NO), numerous studies have been performed in order to understand how sGC transduces the NO signal. However, the structural basis of sGC activation is still not completely elucidated. Spectroscopic and kinetic studies showed that the key step in the activation mechanism was the NO-induced breaking of the iron proximal histidine bond in the so-called 6c-NO to 5c-NO transition. The main breakthrough in the understanding of sGC activation mechanism came, however, from the elucidation of crystal structures for two different prokaryotic heme NO oxygen (HNOX) domains, which are homologues to the sGC heme domain. In this work we present computer simulation results of Thermoanaerobacter tencogensis HNOX that complement these structural studies, yielding molecular explanations to several poorly understood properties of these proteins. Specifically, our results explain the differential ligand binding patterns of the HNOX domains according to the nature of proximal and distal residues. We also show that the natural dynamics of these proteins is intimately related with the proposed conformational dependent activation process, which involves mainly the alphaFbeta1 loop and the alphaA-alphaC distal subdomain. The results from the sGC models also support this view and suggest a key role for the alphaFbeta1 loop in the iron proximal histidine bond breaking process and, therefore, in the sGC activation mechanism. 相似文献
104.
Pituitary adenylate cyclase-activating peptide 38 (PACAP 38) is a neuropeptide that displays several biological effects of interest in the context of airway diseases such as asthma and chronic obstructive pulmonary disease. These effects include inhibition of airway and vascular smooth muscle tone as well as modulation of inflammatory cell activity. However, little is known about the effect of PACAP on granulocytes. The present study was designed to investigate if PACAP and the closely related peptide vasoactive intestinal peptide (VIP) could affect neutrophil migration. A standard 48 well chemotaxis chamber was used to assess the effects of PACAP on N-Formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP)-induced neutrophil chemotaxis and spontaneous random migration. PACAP 38 and VIP inhibited fMLP-induced human neutrophil chemotaxis. Furthermore, both peptides also exhibited a dose-related trend toward inhibiting the spontaneous, unstimulated migration of neutrophils. Since enhanced cell migration in cell chamber systems is reported to correlate with increased invasive properties in vivo, the presented inhibitory effects of PACAP 38 on neutrophil chemotaxis, supports the idea of an anti-inflammatory role for PACAP. This together with the well documented bronchodilatory capacity of PACAP might indicate a role for PACAP-agonists in future treatment of asthma and other inflammatory airway diseases. 相似文献
105.
106.
Marine amoebae from waters of northwest Spain, with comments on a potentially pathogenic euryhaline species 总被引:1,自引:0,他引:1
M C Arias Fernandez E Paniagua Crespo M Marti Mallen M P Penas Ares M L Casro Casas 《The Journal of protozoology》1989,36(3):239-241
Of 17 species of free-living amoebae identified in various samples of salt water, only 1, Acanthamoeba polyphaga, is known to be a potential pathogen. While no deaths occurred when laboratory animals were inoculated with A. polyphaga to test for pathogenicity, the protozoa were present in the brain, liver and lungs of some but not all of the animals. 相似文献
107.
Rannveig Skrunes Einar Svarstad Anna Varberg Reis?ter Hans-Peter Marti Bj?rn Egil Vikse 《PloS one》2016,11(11)
BackgroundIncreased risk of end stage renal disease (ESRD) and death in Norwegian living kidney donors has been reported, most of the donors were related to the recipient. The present study investigates risk of death in first degree relatives of ESRD patients.MethodsThe Norwegian Population Registry, The Norwegian Cause of Death Registry and the Norwegian Renal Registry were linked. All citizens born in Norway, alive in 1960 and with at least one registered first degree relative were included; individuals who died during the first year of life were excluded. A cohort-design was used, ESRD in a first degree relative was the main exposure variable and death and causes of death were the main outcome variables. Cox regression statistics were used to investigate mortality risks.Results5 130 600 individuals were included, 27 508 had at least one first degree relative with ESRD. 828 022 died during follow-up, of whom 4105 had a first degree relative with ESRD. Adjusted hazard ratio (aHR) for death was 1.13 (1.09–1.16) in individuals with a relative with ESRD compared to those without a relative with ESRD. Excluding known hereditary renal disease, aHR decreased to 1.12 (1.09–1.15). Cardiovascular death aHR was 1.15 (1.10–1.21), of which cerebrovascular death 1.34 (1.22–1.50). aHR for death due to non-hereditary renal/ureteric disease was 2.29 (1.81–2.91) with renal failure 1.80 (1.26–2.56) and glomerular disease 5.69 (3.88–8.34) as main contributors. Diabetes mellitus death aHR was 1.68 (1.35–2.10). Absolute mortality risks increased most for the oldest cohorts with excess mortality of 148 per 100.000 person years for the cohort born 1920–39 and 218 for the cohort born 1900–1919.ConclusionsESRD in first degree relatives was associated with increased hazard ratio for death. Death due to cardiovascular disease, renal disease and diabetes mellitus increased the most. 相似文献
108.
Parathyroid ultrastructure after aldehyde fixation, high-pressure freezing, or microwave irradiation 总被引:2,自引:0,他引:2
R Marti P Wild E M Schraner M Mueller H Moor 《The journal of histochemistry and cytochemistry》1987,35(12):1415-1424
Parathyroid cell variants, commonly observed in parathyroid glands fixed by immersion in glutaraldehyde, are believed to be the result of cyclic changes in the course of parathyroid hormone secretion. Immersion of bovine parathyroid glands in a mixture consisting of 1% glutaraldehyde, 1.5% formaldehyde, and 2.5% acrolein, followed by post-fixation in 1% osmium tetroxide, resulted in high uniformity with only one cell variant, whereas the same fixation procedure led to disruption of cell membranes and formation of cell variants in rat parathyroids. Parathyroid glands of both cattle and rats prepared by high-pressure quick-freezing and subsequent freeze-substitution contained only one cell variant. Excellent preservation of the ultrastructure of bovine and rat parathyroids, also exhibiting only one cell variant, was achieved by microwave irradiation in the presence of 2.5% glutaraldehyde in Na-cacodylate followed by post-fixation with OsO4 in Na-cacodylate or s-collidine, both containing Ca2+ and Mg2+. Use of the appropriate buffer, as well as osmication, is essential for successful fixation utilizing microwave energy. The main effects are considered to be heating specimens within sufficient short periods and enhancement of subsequent osmium fixation. The results support the idea, arising after examination of perfusion-fixed parathyroid tissue, that parathyroid cell variants occur during improper aldehyde fixation rather than that they express functional diversity. 相似文献
109.
110.