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991.
Eakin CM Morgan JA Heron SF Smith TB Liu G Alvarez-Filip L Baca B Bartels E Bastidas C Bouchon C Brandt M Bruckner AW Bunkley-Williams L Cameron A Causey BD Chiappone M Christensen TR Crabbe MJ Day O de la Guardia E Díaz-Pulido G DiResta D Gil-Agudelo DL Gilliam DS Ginsburg RN Gore S Guzmán HM Hendee JC Hernández-Delgado EA Husain E Jeffrey CF Jones RJ Jordán-Dahlgren E Kaufman LS Kline DI Kramer PA Lang JC Lirman D Mallela J Manfrino C Maréchal JP Marks K Mihaly J Miller WJ Mueller EM 《PloS one》2010,5(11):e13969
Background
The rising temperature of the world''s oceans has become a major threat to coral reefs globally as the severity and frequency of mass coral bleaching and mortality events increase. In 2005, high ocean temperatures in the tropical Atlantic and Caribbean resulted in the most severe bleaching event ever recorded in the basin.Methodology/Principal Findings
Satellite-based tools provided warnings for coral reef managers and scientists, guiding both the timing and location of researchers'' field observations as anomalously warm conditions developed and spread across the greater Caribbean region from June to October 2005. Field surveys of bleaching and mortality exceeded prior efforts in detail and extent, and provided a new standard for documenting the effects of bleaching and for testing nowcast and forecast products. Collaborators from 22 countries undertook the most comprehensive documentation of basin-scale bleaching to date and found that over 80% of corals bleached and over 40% died at many sites. The most severe bleaching coincided with waters nearest a western Atlantic warm pool that was centered off the northern end of the Lesser Antilles.Conclusions/Significance
Thermal stress during the 2005 event exceeded any observed from the Caribbean in the prior 20 years, and regionally-averaged temperatures were the warmest in over 150 years. Comparison of satellite data against field surveys demonstrated a significant predictive relationship between accumulated heat stress (measured using NOAA Coral Reef Watch''s Degree Heating Weeks) and bleaching intensity. This severe, widespread bleaching and mortality will undoubtedly have long-term consequences for reef ecosystems and suggests a troubled future for tropical marine ecosystems under a warming climate. 相似文献992.
Hugo Aguilar-Díaz Martha Díaz-Gallardo Juan P. Laclette Julio C. Carrero 《PLoS neglected tropical diseases》2010,4(2)
Inhibition of encystment can be conceived as a potentially useful mechanism to block the transmission of Entamoeba histolytica under natural conditions. Unfortunately, amoeba encystment has not been achieved in vitro and drugs inhibiting the formation of cysts are not available. Luminal conditions inducing encystment in vivo are also unknown, but cellular stress such as exposure to reactive oxygen species from immune cells or intestinal microbiota could be involved. A role for certain divalent cations as cofactors of enzymes involved in excystment has also been described. In this study, we show that trophozoite cultures, treated with hydrogen peroxide in the presence of trace amounts of several cations, transform into small-sized spherical and refringent structures that exhibit resistance to different detergents. Ultrastructural analysis under scanning and transmission electron microscopy revealed multinucleated structures (some with four nuclei) with smooth, thick membranes and multiple vacuoles. Staining with calcofluor white, as well as an ELISA binding assay using wheat germ agglutinin, demonstrated the presence of polymers of N-acetylglucosamine (chitin), which is the primary component of the natural cyst walls. Over-expression of glucosamine 6-phosphate isomerase, likely to be the rate-limiting enzyme in the chitin synthesis pathway, was also confirmed by RT-PCR. These results suggest that E. histolytica trophozoites activated encystment pathways when exposed to our treatment. 相似文献
993.
Carmen J. Marsit E. Andres Houseman Brock C. Christensen Luc Gagne Margaret R. Wrensch Heather H. Nelson Joseph Wiemels Shichun Zheng John K. Wiencke Angeline S. Andrew Alan R. Schned Margaret R. Karagas Karl T. Kelsey 《PloS one》2010,5(8)
Approximately 500,000 individuals diagnosed with bladder cancer in the U.S. require routine cystoscopic follow-up to monitor for disease recurrences or progression, resulting in over $2 billion in annual expenditures. Identification of new diagnostic and monitoring strategies are clearly needed, and markers related to DNA methylation alterations hold great promise due to their stability, objective measurement, and known associations with the disease and with its clinical features. To identify novel epigenetic markers of aggressive bladder cancer, we utilized a high-throughput DNA methylation bead-array in two distinct population-based series of incident bladder cancer (n = 73 and n = 264, respectively). We then validated the association between methylation of these candidate loci with tumor grade in a third population (n = 245) through bisulfite pyrosequencing of candidate loci. Array based analyses identified 5 loci for further confirmation with bisulfite pyrosequencing. We identified and confirmed that increased promoter methylation of HOXB2 is significantly and independently associated with invasive bladder cancer and methylation of HOXB2, KRT13 and FRZB together significantly predict high-grade non-invasive disease. Methylation of these genes may be useful as clinical markers of the disease and may point to genes and pathways worthy of additional examination as novel targets for therapeutic treatment. 相似文献
994.
Polymorphisms in HLA‐DQ genes,together with age,sex, and Helicobacter pylori infection,as potential biomarkers for the early diagnosis of gastric cancer
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995.
Isolation by oceanic distance and spatial genetic structure in an overharvested international fishery
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Nathan K. Truelove Stephen J. Box Karl A. Aiken Azra Blythe‐Mallett Erik M. Boman Catherine J. Booker Tamsen T. Byfield Courtney E. Cox Martha H. Davis Gabriel A. Delgado Bob A. Glazer Sarah M. Griffiths Kimani Kitson‐Walters Andy S. Kough Ricardo Pérez Enríquez Richard F. Preziosi Marcia E. Roy Iris Segura‐García Mona K. Webber Allan W. Stoner 《Diversity & distributions》2017,23(11):1292-1300
996.
High glucose induces O‐GlcNAc glycosylation of the vitamin D receptor (VDR) in THP1 cells and in human macrophages derived from monocytes
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997.
Genetic interplay between human longevity and metabolic pathways — a large‐scale eQTL study
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998.
Lisa J. Croner Roslyn Dillon Athit Kao Stefanie N. Kairs Ryan Benz Ib J. Christensen Hans J. Nielsen John E. Blume Bruce Wilcox 《Clinical proteomics》2017,14(1):28
Background
The aim was to improve upon an existing blood-based colorectal cancer (CRC) test directed to high-risk symptomatic patients, by developing a new CRC classifier to be used with a new test embodiment. The new test uses a robust assay format—electrochemiluminescence immunoassays—to quantify protein concentrations. The aim was achieved by building and validating a CRC classifier using concentration measures from a large sample set representing a true intent-to-test (ITT) symptomatic population.Methods
4435 patient samples were drawn from the Endoscopy II sample set. Samples were collected at seven hospitals across Denmark between 2010 and 2012 from subjects with symptoms of colorectal neoplasia. Colonoscopies revealed the presence or absence of CRC. 27 blood plasma proteins were selected as candidate biomarkers based on previous studies. Multiplexed electrochemiluminescence assays were used to measure the concentrations of these 27 proteins in all 4435 samples. 3066 patients were randomly assigned to the Discovery set, in which machine learning was used to build candidate classifiers. Some classifiers were refined by allowing up to a 25% indeterminate score range. The classifier with the best Discovery set performance was successfully validated in the separate Validation set, consisting of 1336 samples.Results
The final classifier was a logistic regression using ten predictors: eight proteins (A1AG, CEA, CO9, DPPIV, MIF, PKM2, SAA, TFRC), age, and gender. In validation, the indeterminate rate of the new panel was 23.2%, sensitivity/specificity was 0.80/0.83, PPV was 36.5%, and NPV was 97.1%.Conclusions
The validated classifier serves as the basis of a new blood-based CRC test for symptomatic patients. The improved performance, resulting from robust concentration measures across a large sample set mirroring the ITT population, renders the new test the best available for this population. Results from a test using this classifier can help assess symptomatic patients’ CRC risk, increase their colonoscopy compliance, and manage next steps in their care.999.
Ane Landajuela Martha Braun Christopher D. A. Rodrigues Alejandro Martínez-Calvo Thierry Doan Florian Horenkamp Anna Andronicos Vladimir Shteyn Nathan D. Williams Chenxiang Lin Ned S. Wingreen David Z. Rudner Erdem Karatekin 《PLoS biology》2021,19(6)
Little is known about mechanisms of membrane fission in bacteria despite their requirement for cytokinesis. The only known dedicated membrane fission machinery in bacteria, fission protein B (FisB), is expressed during sporulation in Bacillus subtilis and is required to release the developing spore into the mother cell cytoplasm. Here, we characterized the requirements for FisB-mediated membrane fission. FisB forms mobile clusters of approximately 12 molecules that give way to an immobile cluster at the engulfment pole containing approximately 40 proteins at the time of membrane fission. Analysis of FisB mutants revealed that binding to acidic lipids and homo-oligomerization are both critical for targeting FisB to the engulfment pole and membrane fission. Experiments using artificial membranes and filamentous cells suggest that FisB does not have an intrinsic ability to sense or induce membrane curvature but can bridge membranes. Finally, modeling suggests that homo-oligomerization and trans-interactions with membranes are sufficient to explain FisB accumulation at the membrane neck that connects the engulfment membrane to the rest of the mother cell membrane during late stages of engulfment. Together, our results show that FisB is a robust and unusual membrane fission protein that relies on homo-oligomerization, lipid binding, and the unique membrane topology generated during engulfment for localization and membrane scission, but surprisingly, not on lipid microdomains, negative-curvature lipids, or curvature sensing.Little is known about how membrane fission occurs in bacteria; this study suggests that the membrane fission protein FisB exploits the unique cellular geometry encountered during sporulation to enable its localization to the fission site through a novel mechanism, where it catalyzes membrane scission. 相似文献
1000.
Andrea J. Cardinal Rebecca Whetten Sanbao Wang Jérôme Auclair David Hyten Perry Cregan Eleni Bachlava Jason Gillman Martha Ramirez Ralph Dewey Greg Upchurch Lilian Miranda Joseph W. Burton 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(1):97-111