全文获取类型
收费全文 | 695篇 |
免费 | 55篇 |
国内免费 | 1篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 6篇 |
2019年 | 15篇 |
2018年 | 9篇 |
2017年 | 11篇 |
2016年 | 11篇 |
2015年 | 26篇 |
2014年 | 25篇 |
2013年 | 31篇 |
2012年 | 45篇 |
2011年 | 50篇 |
2010年 | 33篇 |
2009年 | 22篇 |
2008年 | 37篇 |
2007年 | 44篇 |
2006年 | 47篇 |
2005年 | 44篇 |
2004年 | 26篇 |
2003年 | 30篇 |
2002年 | 30篇 |
2001年 | 8篇 |
2000年 | 18篇 |
1999年 | 10篇 |
1998年 | 10篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 6篇 |
1994年 | 7篇 |
1993年 | 8篇 |
1992年 | 9篇 |
1991年 | 5篇 |
1990年 | 9篇 |
1989年 | 16篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 5篇 |
1974年 | 3篇 |
1972年 | 5篇 |
1971年 | 2篇 |
1969年 | 3篇 |
排序方式: 共有751条查询结果,搜索用时 15 毫秒
101.
102.
Assessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopy 下载免费PDF全文
Previous studies have described both surface morphology and adhesive properties of fungal spores, but little information is currently available on their mechanical properties. In this study, atomic force microscopy (AFM) was used to investigate both surface topography and micromechanical properties of Aspergillus nidulans spores. To assess the influence of proteins covering the spore surface, wild-type spores were compared with spores from isogenic rodA+ and rodA− strains. Tapping-mode AFM images of wild-type and rodA+ spores in air showed characteristic “rodlet” protein structures covering a granular spore surface. In comparison, rodA− spores were rodlet free but showed a granular surface structure similar to that of the wild-type and rodA+ spores. Rodlets were removed from rodA+ spores by sonication, uncovering the underlying granular layer. Both rodlet-covered and rodlet-free spores were subjected to nanoindentation measurements, conducted in air, which showed the stiffnesses to be 110 ± 10, 120 ± 10, and 300 ± 20 N/m and the elastic moduli to be 6.6 ± 0.4, 7.0 ± 0.7, and 22 ± 2 GPa for wild-type, rodA+ and rodA− spores, respectively. These results imply the rodlet layer is significantly softer than the underlying portion of the cell wall. 相似文献
103.
van den Berg MA Albang R Albermann K Badger JH Daran JM Driessen AJ Garcia-Estrada C Fedorova ND Harris DM Heijne WH Joardar V Kiel JA Kovalchuk A Martín JF Nierman WC Nijland JG Pronk JT Roubos JA van der Klei IJ van Peij NN Veenhuis M von Döhren H Wagner C Wortman J Bovenberg RA 《Nature biotechnology》2008,26(10):1161-1168
104.
Nagotu S Krikken AM Otzen M Kiel JA Veenhuis M van der Klei IJ 《Traffic (Copenhagen, Denmark)》2008,9(9):1471-1484
We show that Mdv1 and Caf4, two components of the mitochondrial fission machinery in Saccharomyces cerevisiae , also function in peroxisome proliferation. Deletion of MDV1 , CAF4 or both, however, had only a minor effect on peroxisome numbers at peroxisome-inducing growth conditions, most likely related to the fact that Vps1 – and not Dnm1 – is the key player in peroxisome fission in this organism. In contrast, in Hansenula polymorpha , which has only a Dnm1-dependent peroxisome fission machinery, deletion of MDV1 led to a drastic reduction of peroxisome numbers. This phenotype was accompanied by a strong defect in mitochondrial fission. The MDV1 paralog CAF4 is absent in H. polymorpha . In wild-type H. polymorpha , cells Dnm1–mCherry and green fluorescent protein (GFP)–Mdv1 colocalize in spots that associate with both peroxisomes and mitochondria. Furthermore, Fis1 is essential to recruit Mdv1 to the peroxisomal and mitochondrial membrane. However, formation of GFP–Mdv1 spots – and related to this normal organelle fission – is strictly dependent on the presence of Dnm1. In dnm1 cells, GFP–Mdv1 is dispersed over the surface of peroxisomes and mitochondria. Also, in H. polymorpha mdv1 or fis1 cells, the number of Dnm1–GFP spots is strongly reduced. These spots still associate to organelles but are functionally inactive. 相似文献
105.
In systems ranging from lakes and woodlands to coral reefs, the long-term ecosystem state may often be determined largely
by rare extreme events such as wet ENSO years, droughts, or disease outbreaks. Such events can flip these systems into a contrasting
state that represents either an alternative attractor or a transient that is slow enough to persist even if the frequency
of events that push the system to this state is low. Here we show that escape from herbivores is a mechanism that can play
a role in several state shifts of this kind. This can happen if plants become less susceptible to herbivory as they grow.
Using a model we show that, surprisingly, this mechanism can lead to a situation where a brief resource pulse for plants may
invoke a persistent shift to a high biomass state whereas gradual enrichment to the same resource level is insufficient to
allow such a change. This counterintuitive phenomenon occurs if the numerical response of herbivores is sufficiently slow
to allow the plants to use the resource pulse to escape to a safe biomass at which herbivory is reduced. Our results imply
that rates of environmental change can sometimes be more important than their magnitude. This has many ramifications. On the
conceptual side, our findings suggest that key mechanisms that regulate long-term ecosystem dynamics are easily missed by
the traditional focus of modelers on equilibria. A more practical corollary is that increased climatic variability may have
more profound effects in some ecosystems than gradual change in conditions. 相似文献
106.
Pex14 is the sole component of the peroxisomal translocon that is required for pexophagy 总被引:1,自引:0,他引:1
Pex14 was initially identified as a peroxisomal membrane protein that is involved in docking of the soluble receptor proteins Pex5 and Pex7, which are required for import of PTS1- or PTS2-containing peroxisomal matrix proteins. However, Hansenula polymorpha Pex14 is also required for selective degradation of peroxisomes (pexophagy). Previously we showed that Pex1, Pex4, Pex6 and Pex8 are not required for this process. Here we show that also in the absence of various other peroxins, namely Pex2, Pex10, Pex12, Pex13 and Pex17, pexophagy can normally occur. These peroxins are, like Pex14, components of the peroxisomal translocon. Our data confirm that Pex14 is the sole peroxin that has a unique dual function in two apparent opposite processes, namely peroxisome formation and selective degradation. 相似文献
107.
Phototransduction in flies is the fastest known G protein-coupled signaling cascade, but how this performance is achieved remains unclear. Here, we investigate the mechanism and role of rhodopsin inactivation. We determined the lifetime of activated rhodopsin (metarhodopsin = M( *)) in whole-cell recordings from Drosophila photoreceptors by measuring the time window within which inactivating M( *) by photoreisomerization to rhodopsin could suppress responses to prior illumination. M( *) was inactivated rapidly (tau approximately 20 ms) under control conditions, but approximately 10-fold more slowly in Ca2+-free solutions. This pronounced Ca2+ dependence of M( *) inactivation was unaffected by mutations affecting phosphorylation of rhodopsin or arrestin but was abolished in mutants of calmodulin (CaM) or the CaM-binding myosin III, NINAC. This suggests a mechanism whereby Ca2+ influx acting via CaM and NINAC accelerates the binding of arrestin to M( *). Our results indicate that this strategy promotes quantum efficiency, temporal resolution, and fidelity of visual signaling. 相似文献
108.
Uffe Strandby Knud Ib Christensen Marten Sørensen 《Plant Systematics and Evolution》2009,280(1-2):59-76
This morphometric study of the geographic variation in the Abies religiosa–hickelii–guatemalensis complex is based on samples from 15 Guatemalan and 12 Mexican populations, two populations of A. religiosa s.str. and A. hickelii s.str., and herbarium specimens of A. hickelii, A. vejarii and varieties of A. guatemalensis. The multivariate methods employed were principal components analysis, and UPGMA clustering. The multivariate and univariate analyses based on 231 operational taxonomic units imply that although morphological differences exist distinct morphospecies cannot be recognized within the A. religiosa–hickelii–guatemalensis complex. A Mantel’s test reports that taxonomic dissimilarities are significantly related to geographic distance. We suggest, therefore, that A. religiosa, A. hickelii and A. guatemalensis are merged so that A. hickelii is referred to as A. religiosa subsp. hickelii (Flous & Gaussen) U. Strandby, K.I. Chr. & M. Sørensen, comb. et stat. nov. and A. guatemalensis as A. religiosa subsp. mexicana (Martínez) U. Strandby, K.I. Chr. & M. Sørensen, comb. nov. According to our analyses A. vejarii cannot retain its status as a separate taxon as the material studied is nested within A. religiosa subsp. mexicana. 相似文献
109.
Sarian Kosten Gissell Lacerot Erik Jeppesen David da Motta Marques Egbert H. van Nes Nestor Mazzeo Marten Scheffer 《Ecosystems》2009,12(7):1117-1129
A positive feedback between submerged vegetation and water clarity forms the backbone of the alternative state theory in shallow
lakes. The water clearing effect of aquatic vegetation may be caused by different physical, chemical, and biological mechanisms
and has been studied mainly in temperate lakes. Recent work suggests differences in biotic interactions between (sub)tropical
and cooler lakes might result in a less pronounced clearing effect in the (sub)tropics. To assess whether the effect of submerged
vegetation changes with climate, we sampled 83 lakes over a gradient ranging from the tundra to the tropics in South America.
Judged from a comparison of water clarity inside and outside vegetation beds, the vegetation appeared to have a similar positive
effect on the water clarity across all climatic regions studied. However, the local clearing effect of vegetation decreased
steeply with the contribution of humic substances to the underwater light attenuation. Looking at turbidity on a whole-lake
scale, results were more difficult to interpret. Although lakes with abundant vegetation (>30%) were generally clear, sparsely
vegetated lakes differed widely in clarity. Overall, the effect of vegetation on water clarity in our lakes appears to be
smaller than that found in various Northern hemisphere studies. This might be explained by differences in fish communities
and their relation to vegetation. For instance, unlike in Northern hemisphere studies, we find no clear relation between vegetation
coverage and fish abundance or their diet preference. High densities of omnivorous fish and coinciding low grazing pressures
on phytoplankton in the (sub)tropics may, furthermore, weaken the effect of vegetation on water clarity. 相似文献
110.