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91.
92.
We describe an unusual case of chondroblastoma of the rib, initially presenting as a mediastinal mass eroding a vertebra, in which the preoperative diagnosis was made by fine needle aspiration (FNA) cytology and confirmed by histology and electron microscopy of the surgical specimen. Cytologic study of the smears revealed osteoclastlike giant cells and dishesive, mononucleate tumor cells; sections of the paraffin-embedded, aspirated material showed the chondroid matrix and typical chicken wire calcific deposits. Supporting diagnostic evidence was provided by immunohistochemical demonstration of S-100 protein. Unusual features were the presence of intranuclear pseudoinclusions and cytoplasmic granular deposits, which proved to contain iron on histochemical staining, ultrastructural morphology and x-ray analysis. This case emphasizes the value of FNA cytology in providing a correct diagnosis of chondroblastoma as well as the utility of embedding the aspirated material for histologic, immunohistochemical and ultrastructural studies. 相似文献
93.
Longitudinal study on the occurrence in pigs of colistin‐resistant Escherichia coli carrying mcr‐1 following the cessation of use of colistin 下载免费PDF全文
94.
Targeting the phosphatidylinositol 3‐kinase/Akt/mechanistic target of rapamycin signaling pathway in B‐lineage acute lymphoblastic leukemia: An update 下载免费PDF全文
95.
Phosphatidylinositol 3‐kinase inhibition potentiates glucocorticoid response in B‐cell acute lymphoblastic leukemia 下载免费PDF全文
Cecilia Evangelisti Alessandra Cappellini Mariana Oliveira Rita Fragoso João T. Barata Alice Bertaina Franco Locatelli Carolina Simioni Luca M. Neri Francesca Chiarini Annalisa Lonetti Francesca Buontempo Ester Orsini Andrea Pession Lucia Manzoli Alberto Maria Martelli Camilla Evangelisti 《Journal of cellular physiology》2018,233(3):1796-1811
96.
97.
Vittorio Grill Marina Zweyer Renato Bareggi Alberto M. Martelli Marisa Basa Paola Narducci 《Biotechnic & histochemistry》1995,70(2):75-80
In this report we describe a simple and rapid staining technique for cartilage and bone embedded in Araldite. Semitbin sections of embryonic vertebrae obtained from 15 to 17 day mouse fetuses were stained using an aqueous solution 0.25% with respect to methylene blue, 0.25% with respect to azure A, and 0.5% with respect to Na2 CO3, then counterstained with 1% aqueous pararosaniline chloride (MAP). Results were compared with toluidine blue stained sections. MAP permitted good discrimination of developmental stages of both cells and extracellular matrix within vertebral ossification centers during endochondral ossification. The technique is simple, rapid and applicable to plastic embedded sections, and can be used prior to ultrastructural examination. 相似文献
98.
Luca M. Neri Frank O. Fackelmayer Marina Zweyer Terumi Kohwi-Shigematsu Alberto M. Martelli 《Chromosoma》1997,106(2):81-93
The nuclear matrix, a proteinaceous entity thought to be a scaffolding structure that determines the higher order organization
of eukaryotic chromatin, is usually prepared from intact nuclei by a series of extraction steps. In most cell types investigated,
the nuclear matrix does not spontaneously resist these extractions, but must rather be stabilized before the application of
extracting agents such as high salt solutions or lithium diiodosalicylate. We have examined the effect of two widely used
stabilization procedures on the localization of nuclear matrix proteins. Four individual polypeptides were studied, all of
which are scaffold or matrix-associated region (S/MAR)-binding proteins: SATB1, SAF-A/hnRNP-U, NuMA , and topoisomerase II
α. Nuclei were isolated from K562 human erythroleukemia cells in a buffer containing spermine, spermidine, KCl and EDTA, and
the nuclear matrix or scaffold was obtained by extraction with lithium diiodosalicylate after stabilization by heat treatment
(37° or 42°C) or incubation with Cu2+ ions. When the localization of individual proteins was determined by immunofluorescent staining and confocal scanning laser
microscopy, markedly different consequences of the two stabilization strategies became evident, ranging from a total maintenance
of the localization (NuMA and topoisomerase II α) to a marked redistribution (SATB1 and SAF-A/hnRNP-U). Our results seem to
indicate that a reevaluation of stabilization protocols employed for the preparation of the nuclear matrix is desirable, especially
by performing morphological controls.
Received: 22 January 1997; in revised form: 17 February 1997 / Accepted: 21 February 1997 相似文献
99.
Ester S. Ramos Carlos A. Moreira-Filho Yvone A. M. V. A. Vicente Maria A. S. Llorach-Velludo Silvio Tucci Jr. Maria H. O. Duarte Amélia G. Araújo Lucia Martelli 《Human genetics》1996,97(5):596-598
Two 46,XX true hermaphrodites and one XX male without genital ambiguities are reported. They coexist in two generations of the same pedigree, with paternal transmission and in the absence of SRY (sex-determining region, Y chromosome). These familial cases provide evidence to support the hypothesis that these disorders are alternative manifestations of the same genetic defect, probably an autosomal dominant mutation (with incomplete penetrance) or an X-linked mutation (limited by the presence of the Y chromosome). 相似文献
100.
Marco?SazziniEmail author Sara?De Fanti Anna?Cherubini Andrea?Quagliariello Giuseppe?Profiti Pier?Luigi?Martelli Rita?Casadio Chiara?Ricci Massimo?Campieri Alberto?Lanzini Umberto?Volta Giacomo?Caio Claudio?Franceschi Enzo?Spisni Donata?Luiselli 《Genes & nutrition》2016,11(1):15