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41.
42.
We studied the aggregation state of Photosystem II in stacked and unstacked thylakoid membranes from spinach after a quick
and mild solubilization with the non-ionic detergent n-dodecyl-α,D-maltoside, followed by analysis by diode-array-assisted gel filtration chromatography and electron microscopy.
The results suggest that Photosystem II (PS II) isolates either as a paired, appressed membrane fragment or as a dimeric PS
II-LHC II supercomplex upon mild solubilization of stacked thylakoid membranes or PS II grana membranes, but predominantly
as a core monomer upon mild solubilization of unstacked thylakoid membranes. Analysis of paired grana membrane fragments reveals
that the number of PS II dimers is strongly reduced in single membranes at the margins of the grana membrane fragments. We
suggest that unstacking of thylakoid membranes results in a spontaneous disintegration of the PS II-LHC II supercomplexes
into separated PS II core monomers and peripheral light-harvesting complexes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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de Melo AC d'Avila-Levy CM Branquinha MH Vermelho AB 《Experimental parasitology》2002,102(3-4):150-156
The extracellular metalloproteinases of the insect trypanosomatid Crithidia guilhermei were characterized through the incorporation of different protein substrates (gelatin, casein, haemoglobin, and bovine serum albumin) into SDS-PAGE. Two gelatinases (60 and 80 kDa) showed ability to degrade casein as well and a 67-kDa enzyme presented the broadest specificity since it was also able to degrade casein and haemoglobin. Besides the 67-kDa extracellular proteinases detected on haemoglobin-SDS-PAGE, a 43-kDa haemoglobinase was only observed with this substrate. All C. guilhermei proteinases were incapable of using bovine serum albumin. C. guilhermei was also grown in four different culture media and the best proteinase production was reached using yeast extract-peptone medium containing glucose as the major carbon source. The results point to the importance of the use of distinct culture media and proteinaceous substrates on the characterization of extracellular proteolytic activities in trypanosomatids, since alterations in growth conditions and methods of detection could lead to distinct proteolytic profiles. 相似文献
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Bruce E. Maryanoff Michael J. Costanzo Ricahrd P. Shank James J. Schupsky Marta E. Ortegon Jeffry L. Vaught 《Bioorganic & medicinal chemistry letters》1993,3(12):2653-2656
Exploration structure-activity relationships surrounding the clinically effective antiepileptic drug topiramate (1) led to a series of potent anticonvulsants with a 4,5-cyclic sulfate or 4,5-cycli sulfite functionality. Key derivative 2 (RWJ-37947) is ca. 8 times more potent than topiramate in mice; it also features a long duration of action and a very favorable neurotoxicity index. 相似文献
49.
Background
In gene expression studies a key role is played by the so called "pre-processing", a series of steps designed to extract the signal and account for the sources of variability due to the technology used rather than to biological differences between the RNA samples. At the moment there is no commonly agreed gold standard pre-processing method and each researcher has the responsibility to choose one method, incurring the risk of false positive and false negative features arising from the particular method chosen. 相似文献50.
The present work proposes new boundaries for the current submediterranean territories of the Iberian Peninsula, defining them
at the smallest scale attempted to date. The boundaries proposed are not sharp divisions but somewhat ‘gradual’, reflecting
the transitional nature of the territories they encompass. Climate change predictions were used to estimate how the distribution
of these submediterranean regions might change in the near future. The maps constructed are based on the distribution of marcescent
Quercus species—trees that characterise the submediterranean plant landscape where they form the main forest communities. To determine
their climatic range, the distribution of different types of Iberian oak forest was represented in ‘climate diagrams’ (ordination
diagrams derived from principal components analysis), both in terms of individual species and groups of species based on leaf
ecophysiological type, i.e. marcescent (Submediterranean), sclerophyllous (Mediterranean), semideciduous (Mediterranean) and
deciduous (Eurosiberian). The climate range of each type of forest was determined, and the means of representative climate
variables are analysed by one way ANOVA. The variables differentiating the forest groups were also examined by discriminant
analysis. The range of the climate variables found to be associated with the majority of marcescent forests was used to determine
the distribution of territories throughout the Peninsula with the same conditions (i.e. whether marcescent forests were present
or not), thus providing a map of the Iberian submediterranean territories. Predictions of climate change were used to investigate
possible climate-induced modifications in the boundaries of these territories in the near future. The patterns obtained show
dramatic reductions in the extension of the Iberian submediterranean environment. Submediterranean conditions will probably
disappear from the areas where they currently reign, and it seems unlikely that any new, large submediterranean areas will
form by displacement towards higher altitudes. The outlook for the unique submediterranean vegetation of the Iberian Peninsula
is gloomy.
相似文献
Helios Sainz-OlleroEmail: |