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101.
Edyta Rytelewska Katarzyna Kisielewska Marta Kiezun Kamil Dobrzyn Marlena Gudelska Agnieszka Rak Joelle Dupont Barbara Kaminska Tadeusz Kaminski Nina Smolinska 《Molecular reproduction and development》2020,87(7):739-762
Recent studies have demonstrated that chemerin participates in the regulation of female reproductive function at the level of the ovaries. Due to the lack of data concerning the presence of the chemerin system (chemerin and its receptors: CMKLR1, GPR1, CCRL2) in the ovaries of pigs, one of the most economically important livestock species, the aim of this study was to investigate the expression and localization of chemerin and its receptors in the ovaries of prepubertal and mature gilts. We also aimed to examine the concentrations of chemerin in the follicular fluid of prepubertal and mature animals. In the present study, we have demonstrated the expression patterns of chemerin system components in the porcine follicles of different sizes of prepubertal and mature animals, as well as in corpora lutea of mature gilts during the estrous cycle and early pregnancy. The obtained results suggest that the expression of chemerin system components is influenced by the reproductive stage, cell type, and the hormonal status of gilts (the estrous cycle/pregnancy). We have also presented the localization of the chemerin system components in various ovarian structures, and also showed changes in the concentration of chemerin in the follicular fluid of pigs. The presented findings not only confirm that chemerin is produced locally in the porcine ovary but they also demonstrate that chemerin directly affects ovarian cells, as confirmed by the presence of chemerin receptors in all ovarian structures. Therefore, chemerin appears to be an important intra‐ovarian factor that could regulate ovary function in pigs. 相似文献
102.
103.
Julen Astigarraga Enrique Andivia Miguel A. Zavala Antonio Gazol Vernica Cruz‐Alonso Sergio M. Vicente‐Serrano Paloma Ruiz‐Benito 《Global Change Biology》2020,26(9):5063-5076
Climate and forest structure are considered major drivers of forest demography and productivity. However, recent evidence suggests that the relationships between climate and tree growth are generally non‐stationary (i.e. non‐time stable), and it remains uncertain whether the relationships between climate, forest structure, demography and productivity are stationary or are being altered by recent climatic and structural changes. Here we analysed three surveys from the Spanish Forest Inventory covering c. 30 years of information and we applied mixed and structural equation models to assess temporal trends in forest structure (stand density, basal area, tree size and tree size inequality), forest demography (ingrowth, growth and mortality) and above‐ground forest productivity. We also quantified whether the interactive effects of climate and forest structure on forest demography and above‐ground forest productivity were stationary over two consecutive time periods. Since the 1980s, density, basal area and tree size increased in Iberian forests, and tree size inequality decreased. In addition, we observed reductions in ingrowth and growth, and increases in mortality. Initial forest structure and water availability mainly modulated the temporal trends in forest structure and demography. The magnitude and direction of the interactive effects of climate and forest structure on forest demography changed over the two time periods analysed indicating non‐stationary relationships between climate, forest structure and demography. Above‐ground forest productivity increased due to a positive balance between ingrowth, growth and mortality. Despite increasing productivity over time, we observed an aggravation of the negative effects of climate change and increased competition on forest demography, reducing ingrowth and growth, and increasing mortality. Interestingly, our results suggest that the negative effects of climate change on forest demography could be ameliorated through forest management, which has profound implications for forest adaptation to climate change. 相似文献
104.
Anna Barbanti Hector Torrado Enrique Macpherson Luca Bargelloni Rafaella Franch Carlos Carreras Marta Pascual 《Molecular ecology resources》2020,20(3):795-806
High‐throughput sequencing has revolutionized population and conservation genetics. RAD sequencing methods, such as 2b‐RAD, can be used on species lacking a reference genome. However, transferring protocols across taxa can potentially lead to poor results. We tested two different IIB enzymes (AlfI and CspCI) on two species with different genome sizes (the loggerhead turtle Caretta caretta and the sharpsnout seabream Diplodus puntazzo) to build a set of guidelines to improve 2b‐RAD protocols on non‐model organisms while optimising costs. Good results were obtained even with degraded samples, showing the value of 2b‐RAD in studies with poor DNA quality. However, library quality was found to be a critical parameter on the number of reads and loci obtained for genotyping. Resampling analyses with different number of reads per individual showed a trade‐off between number of loci and number of reads per sample. The resulting accumulation curves can be used as a tool to calculate the number of sequences per individual needed to reach a mean depth ≥20 reads to acquire good genotyping results. Finally, we demonstrated that selective‐base ligation does not affect genomic differentiation between individuals, indicating that this technique can be used in species with large genome sizes to adjust the number of loci to the study scope, to reduce sequencing costs and to maintain suitable sequencing depth for a reliable genotyping without compromising the results. Here, we provide a set of guidelines to improve 2b‐RAD protocols on non‐model organisms with different genome sizes, helping decision‐making for a reliable and cost‐effective genotyping. 相似文献
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106.
Forecasting the effects of climate change on species and populations is a fundamental goal of conservation biology, especially for montane endemics which seemingly are under the greatest threat of extinction given their association with cool, high elevation habitats. Species distribution models (also known as niche models) predict where on the landscape there is suitable habitat for a species of interest. Correlative niche modeling, the most commonly employed approach to predict species' distributions, relies on correlations between species' localities and current environmental data. This type of model could spuriously forecast less future suitable habitat because species' current distributions may not adequately represent their thermal tolerance, and future climate conditions may not be analogous to current conditions. We compared the predicted distributions for three montane species of Plethodon salamanders in the southern Appalachian Mountains of North America using a correlative modeling approach and a mechanistic model. The mechanistic model incorporates species-specific physiology, morphology and behavior to predict an annual energy budget on the landscape. Both modeling approaches performed well at predicting the species' current distributions and predicted that all species could persist in habitats at higher elevation through 2085. The mechanistic model predicted more future suitable habitat than the correlative model. We attribute these differences to the mechanistic approach being able to model shifts in key range-limiting biological processes (changes in surface activity time and energy costs) that the correlative approach cannot. Choice of global circulation model (GCM) contributed significantly to distribution predictions, with a tenfold difference in future suitability based on GCM, indicating that GCM variability should be either directly included in models of species distributions or, indirectly, through the use of multi-model ensemble averages. Our results indicate that correlative models are over-predicting habitat loss for montane species, suggesting a critical need to incorporate mechanisms into forecasts of species' range dynamics. 相似文献
107.
Ecological memory describes how antecedent conditions drive the dynamics of an ecological system. Palaeoecological records are paramount to understand ecological memory at millennial time-scales, but the concept is widely neglected in the literature, and a formal approach is lacking. Here, we fill such a gap by introducing a quantitative framework for ecological memory in palaeoecology, and assessing how data constraints and taxa traits shape ecological memory patterns. We simulate the population dynamics and pollen abundance of 16 virtual taxa with different life and niche traits as a response to an environmental driver. The data is processed to mimic a realistic sediment deposition and sampled at increasing depth intervals. We quantify ecological memory with Random Forests, and assess how data properties and taxa traits shape ecological memory. We find that life-span and niche features modulate the relative importance of the antecedent values of the driver and the pollen abundance over periods of 240 yr and longer. Additionally, we find that accumulation rate and decreasing pollen-sampling resolution inflate the importance of antecedent pollen abundance. Our results suggest that: 1) ecological memory patterns are sensitive to varying accumulation rates. A better understanding on the numerical basis of this effect may enable the assimilation of ecological memory concepts and methods in palaeoecology; 2) incorporating niche theory and models is essential to better understand the nature of ecological memory patterns at millennial time-scales. 3) Long-lived generalist taxa are highly decoupled from the environmental signal. This finding has implications on how we interpret the abundance-environment relationship of real taxa with similar traits, and how we use such knowledge to forecast their distribution or reconstruct past climate. 相似文献
108.
Felde Vivian A. Flantua Suzette G. A. Jenks Cathy R. Benito Blas M. de Beaulieu Jacques-Louis Kuneš Petr Magri Donatella Nalepka Dorota Risebrobakken Bjørg ter Braak Cajo J. F. Allen Judy R. M. Granoszewski Wojciech Helmens Karin F. Huntley Brian Kondratienė Ona Kalniņa Laimdota Kupryjanowicz Mirosława Malkiewicz Małgorzata Milner Alice M. Nita Małgorzata Noryśkiewicz Bożena Pidek Irena A. Reille Maurice Salonen J. Sakari Šeirienė Vaida Winter Hanna Tzedakis Polychronis C. Birks H. John B. 《Vegetation History and Archaeobotany》2020,29(1):101-109
Vegetation History and Archaeobotany - The Eemian interglacial represents a natural experiment on how past vegetation with negligible human impact responded to amplified temperature changes... 相似文献
109.
Yara Tibiriçá Marta Pola Deneb Ortigosa Juan Lucas Cervera 《Journal of Zoological Systematics and Evolutionary Research》2020,58(1):230-261
The similarities and variations in nudibranch species of the “Chromodoris quadricolor group” (Heterobranchia, Nudibranchia) have historically created identification problems among both nudibranch enthusiasts and experts. In this study, we combine molecular genetic analyses using one nuclear gene (histone h3) and two mitochondrial genes (cytochrome c oxidase subunit I and ribosomal 16S RNA) with morphological data to ameliorate the identification of specimens from this complex in East Africa. We include a detailed examination of polymorphisms within the group. As a result, Chromodoris boucheti is synonymized with Chromodoris lochi, and two new species are described, Chromodoris celinae sp. nov. and Chromodoris helium sp. nov. Chromodoris celinae sp. nov. is a common shallow water species that was previously misidentified as C. hamiltoni. Chromodoris helium sp. nov. is a species that appears to be restricted to depths below 30 m. This study agrees with previous research indicating the recent divergence of the genus Chromodoris. 相似文献
110.
Stefanie J. Müller‐Schüssele Ren Wang Desire D. Gütle Jill Romer Marta Rodriguez‐Franco Martin Scholz Felix Buchert Volker M. Lüth Stanislav Kopriva Peter Drmann Markus Schwarzlnder Ralf Reski Michael Hippler Andreas J. Meyer 《The Plant journal : for cell and molecular biology》2020,103(3):1140-1154
Thiol‐based redox‐regulation is vital for coordinating chloroplast functions depending on illumination and has been throroughly investigated for thioredoxin‐dependent processes. In parallel, glutathione reductase (GR) maintains a highly reduced glutathione pool, enabling glutathione‐mediated redox buffering. Yet, how the redox cascades of the thioredoxin and glutathione redox machineries integrate metabolic regulation and detoxification of reactive oxygen species remains largely unresolved because null mutants of plastid/mitochondrial GR are embryo‐lethal in Arabidopsis thaliana. To investigate whether maintaining a highly reducing stromal glutathione redox potential (EGSH) via GR is necessary for functional photosynthesis and plant growth, we created knockout lines of the homologous enzyme in the model moss Physcomitrella patens. In these viable mutant lines, we found decreasing photosynthetic performance and plant growth with increasing light intensities, whereas ascorbate and zeaxanthin/antheraxanthin levels were elevated. By in vivo monitoring stromal EGSH dynamics, we show that stromal EGSH is highly reducing in wild‐type and clearly responsive to light, whereas an absence of GR leads to a partial glutathione oxidation, which is not rescued by light. By metabolic labelling, we reveal changing protein abundances in the GR knockout plants, pinpointing the adjustment of chloroplast proteostasis and the induction of plastid protein repair and degradation machineries. Our results indicate that the plastid thioredoxin system is not a functional backup for the plastid glutathione redox systems, whereas GR plays a critical role in maintaining efficient photosynthesis. 相似文献