全文获取类型
收费全文 | 175篇 |
免费 | 12篇 |
国内免费 | 43篇 |
专业分类
230篇 |
出版年
2024年 | 3篇 |
2023年 | 1篇 |
2022年 | 4篇 |
2021年 | 8篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 2篇 |
2017年 | 5篇 |
2016年 | 9篇 |
2015年 | 8篇 |
2014年 | 17篇 |
2013年 | 11篇 |
2012年 | 10篇 |
2011年 | 13篇 |
2010年 | 12篇 |
2009年 | 15篇 |
2008年 | 12篇 |
2007年 | 11篇 |
2006年 | 12篇 |
2005年 | 8篇 |
2004年 | 7篇 |
2003年 | 7篇 |
2002年 | 7篇 |
2001年 | 3篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1990年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1976年 | 1篇 |
1974年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有230条查询结果,搜索用时 15 毫秒
151.
小叶臭黄皮叶挥发油化学成分的研究 总被引:8,自引:0,他引:8
采用水蒸气蒸馏法提取小叶臭黄皮叶挥发油,运用毛细管气相色谱-质谱联用法对挥发油成分进行了分析,共分离出84个峰,鉴定了其中的66种成分,所鉴定成分占挥发油总量的94.52%.其主要化学成分为α-芹子烯(15.76%)、石竹烯(15.05%)、β-芹子烯(9.54%)、α-蒎烯(6.43%)和α-石竹烯(5.39%)等. 相似文献
152.
Michaela Georgescu Camille Sabongui Adina Del Corpo Lina Marsan James G. Pfaus 《Hormones and behavior》2009,56(4):450-456
Vaginocervical stimulation (VCS) induces the immediate-early gene product Fos in the ventromedial hypothalamus (VMH) of female rats. However, this induction is lower in ovariectomized rats that receive estradiol benzoate (EB) and progesterone (P) relative to an oil vehicle. We have observed that a substantial proportion of cells activated in the VMH by VCS stain for glutamate, and infusions of glutamate or its selective receptor agonists to the VMH inhibit both appetitive and consummatory sexual behaviors in females. This raises the possibility that VCS activates an inhibitory glutamate system in the VMH, and that ovarian steroids blunt the activation, although it is not known whether EB or P, alone or in combination, lead to this effect. The present experiment examined the ability of VCS to induce Fos in glutamate neurons in the VMH of ovariectomized rats under 4 hormonal regimens: oil, EB alone, P alone, or EB + P, following 1 or 50 distributed VCSs administered with a lubricated glass rod over the course of 1 h. Treatment with EB or P alone significantly reduced the number of glutamate neurons activated by 1 VCS, with P being more effective than EB. Treatment with EB + P also produced a significant reduction, but not to the extent of EB or P alone. Although EB and P work in synergy to activate sexual behavior in female rats, actions of EB or P alone are sufficient to blunt the ability of VCS to activate glutamate neurons in the VMH. It thus appears that ovarian steroids may “disinhibit” sexual responding, in part, by dampening the ability of VCS to activate glutamate neurons in the VMH. In turn, this may allow females to receive a sufficient number of intromissions for the activation of sexual reward and the facilitation of pregnancy. 相似文献
153.
MAI Tiejun* WANG Xin* ZHANG Zhiwen XIN Dianqi NA Yanqun & GUO Yinglu . Institute of Urology First Hospital of Peking University Beijing China . Department of General Surgery First Hospital of Peking University Beijing China . Department of Physiology Peking University Beijing China 《中国科学:生命科学英文版》2004,(5)
154.
155.
Evolutionary rates for tuf genes in endosymbionts of aphids 总被引:4,自引:1,他引:4
The gene encoding elongation factor Tu (tuf) in aphid endosymbionts (genus
Buchnera) evolves at rates of 1.3 x 10(-10) to 2.5 x 10(-10) nonsynonymous
substitutions and 3.9 x 10(-9) to 8.0 x 10(-9) synonymous substitutions per
position per year. These rates, which are at present among the most
reliable substitution rates for protein-coding genes of bacteria, have been
obtained by calibrating the nodes in the phylogenetic tree produced from
the Buchnera EF-Tu sequences using divergence times for the corresponding
ancestral aphid hosts. We also present data suggesting that the rates of
nonsynonymous substitutions are significantly higher in the endosymbiont
lineages than in the closely related free-living bacteria Escherichia coli
and Salmonella typhimurium. Synonymous substitution rates for Buchnera
approximate estimated mutation rates for E. coli and S. typhimurium, as
expected if synonymous changes act as neutral mutations in Buchnera. We
relate the observed differences in substitution frequencies to the absence
of selective codon preferences in Buchnera and to the influence of Muller's
ratchet on small asexual populations.
相似文献
156.
157.
158.
不同真菌纤维素酶一些生物化学性质的比较 总被引:10,自引:0,他引:10
比较了属于6个属,即青霉(Penicillium)、曲霉(Aspergillus)、木霉(Trichoderma)、漆斑菌(Myrothecium)、镰孢霉(Fusarium)和腐质霉(Humicola),总计9株真菌在麦麸液体培养基中所产纤维素酶的一些生物化学性质。此外,还比较了这9株真菌在麦麸固体培养基上所产纤维素酶的活力以及对脱脂棉、水曲柳木屑、水曲柳木粉、废报纸和玉米芯糠醛废渣的糖化能力。 相似文献
159.
A candidate gene association study for nine economically important traits in Italian Holstein cattle 总被引:1,自引:0,他引:1
L. Fontanesi D. G. Calò G. Galimberti R. Negrini R. Marino A. Nardone P. Ajmone‐Marsan V. Russo 《Animal genetics》2014,45(4):576-580
We genotyped 58 single nucleotide polymorphisms (SNPs) in 25 candidate genes in about 800 Italian Holstein sires. Fifty‐six (minor allele frequency >0.02) were used to evaluate their association with single traits: milk yield (MY), milk fat yield (FY), milk protein yield (PY), milk fat percentage (FP), milk protein percentage (PP), milk somatic cell count (MSCC); and complex indexes: longevity, fertility and productivity–functionality type (PFT), using deregressed proofs, after adjustment for familial relatedness. Thirty‐two SNPs were significantly associated (proportion of false positives <0.05) with different traits: 16 with MSCC, 15 with PY, 14 with MY, 12 with PFT, eight with longevity, eight with FY, eight with PP, five with FP and two with fertility. In particular, a SNP in the promoter region of the PRLR gene was associated with eight of nine traits. DGAT1 polymorphisms were highly associated with FP and FY. Casein gene markers were associated with several traits, confirming the role of the casein gene cluster in affecting milk yield, milk quality and health traits. Other SNPs in genes located on chromosome 6 were associated with PY, PP, PFT, MY (PPARGC1A) and MSCC (KIT). This latter association may suggest a biological link between the degree of piebaldism in Holstein and immunological functions affecting somatic cell count and mastitis resistance. Other significant SNPs were in the ACACA, CRH, CXCR1, FASN, GH1, LEP, LGB (also known as PAEP), MFGE8, SRC, TG, THRSP and TPH1 genes. These results provide information that can complement QTL mapping and genome‐wide association studies in Holstein. 相似文献
160.
滇南红厚壳种子油的脂肪酸成分 总被引:25,自引:0,他引:25
纳智 《热带亚热带植物学报》2005,13(6):505-506
应用GC-MS联用技术对云南省西双版纳产的滇南红厚壳(Calophyllumpolyanthum)种子油的脂肪酸成分进行分析.检出12种脂肪酸成分,占总量的99.39%,主要是亚油酸(38.75%),棕榈酸(22.42%),油酸(22.11%)和硬脂酸(9.81%). 相似文献