全文获取类型
收费全文 | 1583篇 |
免费 | 159篇 |
国内免费 | 1篇 |
专业分类
1743篇 |
出版年
2023年 | 9篇 |
2022年 | 19篇 |
2021年 | 37篇 |
2020年 | 25篇 |
2019年 | 41篇 |
2018年 | 31篇 |
2017年 | 34篇 |
2016年 | 58篇 |
2015年 | 55篇 |
2014年 | 71篇 |
2013年 | 86篇 |
2012年 | 91篇 |
2011年 | 81篇 |
2010年 | 78篇 |
2009年 | 55篇 |
2008年 | 68篇 |
2007年 | 62篇 |
2006年 | 72篇 |
2005年 | 58篇 |
2004年 | 60篇 |
2003年 | 52篇 |
2002年 | 62篇 |
2001年 | 61篇 |
2000年 | 60篇 |
1999年 | 46篇 |
1998年 | 26篇 |
1997年 | 25篇 |
1996年 | 10篇 |
1995年 | 13篇 |
1994年 | 12篇 |
1993年 | 10篇 |
1992年 | 24篇 |
1991年 | 32篇 |
1990年 | 33篇 |
1989年 | 31篇 |
1988年 | 23篇 |
1987年 | 8篇 |
1986年 | 12篇 |
1985年 | 8篇 |
1984年 | 9篇 |
1983年 | 14篇 |
1982年 | 4篇 |
1981年 | 8篇 |
1980年 | 12篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1975年 | 8篇 |
1972年 | 4篇 |
1967年 | 3篇 |
排序方式: 共有1743条查询结果,搜索用时 0 毫秒
91.
92.
Ai Yue Lauren Marsh Huan Zhou Alexis Medina Renfu Luo Yaojiang Shi Linxiu Zhang Kaleigh Kenny Scott Rozelle 《PloS one》2016,11(4)
MethodologyWe interviewed sixty caregivers participating in a large survey on child health and nutrition. We conducted three waves of interviews with children’s primary caregivers in seventeen rural villages within four nationally-designated poverty counties in the southern part of Shaanxi Province.ResultsThe qualitative analysis reveals that poor complementary feeding practices are common across our sample. Information gathered from our interviews suggests that complementary feeding practices are impeded by two constraints: absence of understanding topics related to infant health and nutrition under caregivers, as well as inadequate sources of information on these topics. Poverty does not appear to constrain child feeding practices.ConclusionOur results uncover lack of proper knowledge on infant and child nutrition among rural caregivers in China. This situation causes them to fail incorporating micronutrient rich foods in their children''s diet. Age-appropriate complementary feeding can stimulate children’s physical and cognitive development, but in its absence it leads to iron-deficiency anemia. We suggest that steps be taken to educate caregivers to improve complementary feeding of their infants and children. 相似文献
93.
94.
95.
96.
Traditional ecological theory predicts that specialisation can promote speciation; hence, recently derived species are specialists. However, an alternative view is that new species have broad niches, which become narrower and specialised over time. Here, we test these hypotheses using avian brood parasites and three different measures of host specialisation. Brood parasites provide an ideal system in which to investigate the evolution of specialisation, because some exploit more than 40 host species and others specialise on only one. We find that young brood parasite species are smaller and specialise on a narrower range of host sizes, as expected, if specialisation is linked with the generation of new species. Moreover, we show that highly virulent parasites are more specialised, supporting findings in other host–parasite systems. Finally, we demonstrate that different measures of specialisation can lead to different conclusions, and specialisation indices should be designed taking into account the biology of each system. 相似文献
97.
α‐Hemolysin enhances Staphylococcus aureus internalization and survival within mast cells by modulating the expression of β1 integrin 下载免费PDF全文
Oliver Goldmann Lorena Tuchscherr Manfred Rohde Eva Medina 《Cellular microbiology》2016,18(6):807-819
Mast cells (MCs) are important sentinels of the host defence against invading pathogens. We previously reported that Staphylococcus aureus evaded the extracellular antimicrobial activities of MCs by promoting its internalization within these cells via β1 integrins. Here, we investigated the molecular mechanisms governing this process. We found that S. aureus responded to the antimicrobial mediators released by MCs by up‐regulating the expression of α‐hemolysin (Hla), fibronectin‐binding protein A and several regulatory systems. We also found that S. aureus induced the up‐regulation of β1 integrin expression on MCs and that this effect was mediated by Hla‐ADAM10 (a disintegrin and metalloproteinase 10) interaction. Thus, deletion of Hla or inhibition of Hla‐ADAM10 interaction significantly impaired S. aureus internalization within MCs. Furthermore, purified Hla but not the inactive HlaH35L induced up‐regulation of β1 integrin expression in MCs in a dose‐dependent manner. Our data support a model in which S. aureus counter‐reacts the extracellular microbicidal mechanisms of MCs by increasing expression of fibronectin‐binding proteins and by inducing Hla‐ADAM10‐mediated up‐regulation of β1 integrin in MCs. The up‐regulation of bacterial fibronectin‐binding proteins, concomitantly with the increased expression of its receptor β1 integrin on the MCs, resulted in enhanced S. aureus internalization through the binding of fibronectin‐binding proteins to integrin β1 via fibronectin. 相似文献
98.
Structure‐based classification of FAD binding sites: A comparative study of structural alignment tools 下载免费PDF全文
A total of six different structural alignment tools (TM‐Align, TriangleMatch, CLICK, ProBis, SiteEngine and GA‐SI) were assessed for their ability to perform two particular tasks: (i) discriminating FAD (flavin adenine dinucleotide) from non‐FAD binding sites, and (ii) performing an all‐to‐all comparison on a set of 883 FAD binding sites for the purpose of classifying them. For the first task, the consistency of each alignment method was evaluated, showing that every method is able to distinguish FAD and non‐FAD binding sites with a high Matthews correlation coefficient. Additionally, GA‐SI was found to provide alignments different from those of the other approaches. The results obtained for the second task revealed more significant differences among alignment methods, as reflected in the poor correlation of their results and highlighted clearly by the independent evaluation of the structural superimpositions generated by each method. The classification itself was performed using the combined results of all methods, using the best result found for each comparison of binding sites. A number of different clustering methods (Single‐linkage, UPGMA, Complete‐linkage, SPICKER and k‐Means clustering) were also used. The groups of similar binding sites (proteins) or clusters generated by the best performing method were further analyzed in terms of local sequence identity, local structural similarity and conservation of analogous contacts with the FAD ligands. Each of the clusters was characterized by a unique set of structural features or patterns, demonstrating that the groups generated truly reflect the structural diversity of FAD binding sites. Proteins 2016; 84:1728–1747. © 2016 Wiley Periodicals, Inc. 相似文献
99.
M. Linde‐Medina 《Journal of evolutionary biology》2016,29(9):1873-1878
Recent comparative studies have indicated the existence of a common cranial evolutionary allometric (CREA) pattern in mammals and birds, in which smaller species have relatively smaller faces and bigger braincases than larger species. In these studies, cranial allometry was tested using a multivariate regression between shape (described using landmarks coordinates) and size (i.e. centroid size), after accounting for phylogenetic relatedness. Alternatively, cranial allometry can be determined by comparing the sizes of two anatomical parts using a bivariate regression analysis. In this analysis, a slope higher or lower than one indicates the existence of positive or negative allometry, respectively. Thus, in those species that support the CREA ‘rule’, positive allometry is expected for the association between face size and braincase size, which would indicate that larger species have disproportionally larger faces. In this study, I applied these two approaches to explore cranial allometry in 83 Galliformes (Aves, Galloanserae), ranging in mean body weight from 30 g to 2.5 kg. The multivariate regression between shape and centroid size revealed the existence of a significant allometric pattern resembling CREA, whereas the second analysis revealed a negative allometry for beak size and braincase size (i.e. contrary to the CREA ‘rule’, larger galliform species have disproportionally shorter beaks than smaller galliform species). This study suggests that the presence of CREA may be overestimated when using cranium size as the standard measurement. 相似文献
100.