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61.
Janine de Zeeuw Marlies Douwstra Till F. Omansen Ghislain E. Sopoh Christian Johnson Richard O. Phillips Marike Alferink Paul Saunderson Tjip S. Van der Werf Pieter U. Dijkstra Ymkje Stienstra 《PLoS neglected tropical diseases》2014,8(11)
Background
Buruli ulcer is a stigmatising disease treated with antibiotics and wound care, and sometimes surgical intervention is necessary. Permanent limitations in daily activities are a common long term consequence. It is unknown to what extent patients perceive problems in participation in social activities. The psychometric properties of the Participation Scale used in other disabling diseases, such as leprosy, was assessed for use in former Buruli ulcer patients.Methods
Former Buruli ulcer patients in Ghana and Benin, their relatives, and healthy community controls were interviewed using the Participation Scale, Buruli Ulcer Functional Limitation Score, and the Explanatory Model Interview Catalogue to measure stigma. The Participation Scale was tested for the following psychometric properties: discrimination, floor and ceiling effects, internal consistency, inter-item correlation, item-total correlation and construct validity.Results
In total 386 participants (143 former Buruli ulcer patients with their relatives (137) and 106 community controls) were included in the study. The Participation Scale displayed good discrimination between former Buruli ulcer patients and healthy community controls. No floor and ceiling effects were found. Internal consistency (Cronbach''s alpha) was 0.88. In Ghana, mean inter-item correlation of 0.29 and item-total correlations ranging from 0.10 to 0.69 were found while in Benin, a mean inter-item correlation of 0.28 was reported with item-total correlations ranging from −0.08 to 0.79. With respect to construct validity, 4 out of 6 hypotheses were not rejected, though correlations between various constructs differed between countries.Conclusion
The results indicate the Participation Scale has acceptable psychometric properties and can be used for Buruli ulcer patients in Ghana and Benin. Future studies can use this Participation Scale to evaluate the long term restrictions in participation in daily social activities of former BU patients. 相似文献62.
Topology of PhoE porin: the 'eyelet' region 总被引:11,自引:0,他引:11
Marlies Struyvé Jan Visser Henriëtte Adriaanse Roland Benz Jan Tommassen 《Molecular microbiology》1993,7(1):131-140
A model for the topology of the PhoE porin has been proposed according to which the polypeptide traverses the outer membrane sixteen times mostly as amphipathic β-sheets, thereby exposing eight loops at the cell surface. Until now, no evidence has been obtained for the surface exposure of the third loop. Recently, the structure of porin of Rhodobacter capsulatus has been determined. The proposed model of PhoE is very similar to the structure of the R capsulatus porin, which has an ‘eyelet’ region, extending into the interior of the pore. The proposed third external loop of PhoE might form a similar ‘eyelet’ region. To determine the location of the predicted third external loop of PhoE, multiple copies of an oligonucleotide linker encoding an antigenic determinant of VP1 protein of foot-and-mouth disease virus (FMDV) were inserted. All hybrid proteins were properly inserted in the outer membrane. The monoclonal antibody MA11, directed against the linear FMDV epitope, was able to bind only to intact cells expressing a hybrid PhoE protein with at least three copies of the FMDV epitope present. Antibiotic sensitivity tests and single-channel conductance measurements revealed that the insertions influenced the channel size. These results are consistent with a location of the third loop of PhoE within the pore channel. 相似文献
63.
Janine de Zeeuw Till F. Omansen Marlies Douwstra Yves T. Barogui Chantal Agossadou Ghislain E. Sopoh Richard O. Phillips Christian Johnson K. Mohammed Abass Paul Saunderson Pieter U. Dijkstra Tjip S. van der Werf Ymkje Stientstra 《PLoS neglected tropical diseases》2014,8(11)
BackgroundBuruli ulcer may induce severe disabilities impacting on a person''s well-being and quality of life. Information about long-term disabilities and participation restrictions is scanty. The objective of this study was to gain insight into participation restrictions among former Buruli ulcer patients in Ghana and Benin.MethodsIn this cross-sectional study, former Buruli ulcer patients were interviewed using the Participation Scale, the Buruli Ulcer Functional Limitation Score to measure functional limitations, and the Explanatory Model Interview Catalogue to measure perceived stigma. Healthy community controls were also interviewed using the Participation Scale. Trained native interviewers conducted the interviews. Former Buruli ulcer patients were eligible for inclusion if they had been treated between 2005 and 2011, had ended treatment at least 3 months before the interview, and were at least 15 years of age.ResultsIn total, 143 former Buruli ulcer patients and 106 community controls from Ghana and Benin were included in the study. Participation restrictions were experienced by 67 former patients (median score, 30, IQR; 23;43) while 76 participated in social life without problems (median score 5, IQR; 2;9). Most restrictions encountered related to employment. Linear regression showed being female, perceived stigma, functional limitations, and larger lesions (category II) as predictors of more participation restrictions.ConclusionPersisting participation restrictions were experienced by former BU patients in Ghana and Benin. Most important predictors of participation restrictions were being female, perceived stigma, functional limitations and larger lesions. 相似文献
64.
The integration of alien plants in mutualistic plant–hummingbird networks across the Americas: the importance of species traits and insularity 下载免费PDF全文
Pietro K. Maruyama Jeferson Vizentin‐Bugoni Jesper Sonne Ana M. Martín González Matthias Schleuning Andréa C. Araujo Andrea C. Baquero Juliana Cardona Paola Cardona Peter A. Cotton Glauco Kohler Carlos Lara Tiago Malucelli Oscar Humberto Marín‐Gómez Jeff Ollerton Ana M. Rui Allan Timmermann Isabela G. Varassin Thais B. Zanata Carsten Rahbek Marlies Sazima Bo Dalsgaard 《Diversity & distributions》2016,22(6):672-681
65.
Tetyana Chumak Lukas Rüttiger Sze Chim Lee Dario Campanelli Annalisa Zuccotti Wibke Singer Jiří Popelář Katja Gutsche Hyun-Soon Geisler Sebastian Philipp Schraven Mirko Jaumann Rama Panford-Walsh Jing Hu Thomas Schimmang Ulrike Zimmermann Josef Syka Marlies Knipper 《Molecular neurobiology》2016,53(8):5607-5627
66.
Mauricio Fernández Otárola Marlies Sazima Vera N. Solferini 《Trees - Structure and Function》2016,30(6):2041-2049
Key message
Congeneric species showed similar stem and crown allometry, but differed in crown dimensions indicating that crown size is adaptive and variable despite mechanical restrictions.Abstract
Morphological adaptations favor differential use of the space in tropical trees, but the variability in stem and crown allometry can be constrained by phylogenetic and mechanical factors. In addition, dioecious species show marked differences in their energy requirements related to reproduction, but little information is available about the role of shape and allometry on differential acquisition of energy between the sexes. We studied the stem and crown dimensions of congeneric dioecious trees to determine if there are: (i) differences in the allometry between the sexes, (ii) different average sizes among sympatric species, and (iii) differences in stem and crown allometry between sympatric and allopatric species. Two pairs of sympatric Virola (Myristicaceae) in Brazil and Costa Rica were studied. SMA regression models were used to investigate allometric relationships between diameter at breast height (DBH) and tree height, and between DBH and crown volume (CV). No sexual dimorphism in stem and crown morphology was observed in this study, indicating that differences in resource allocation for reproduction between the sexes do not impact the stem and crown structure in these species. Overall, low variability among the species was observed. Only one species differed in stem allometry and none differed in crown allometry. CV differed between sympatric species. Stem and crown allometry are related to structural stability and our results support similar mechanical restriction for these species. The ecological significance of differences in CV among canopy species remains to be explored.67.
Marlies Fischer Mandeep Kang Nicholas Pj Brindle 《Protein science : a publication of the Protein Society》2016,25(2):352-359
Directed evolution is a powerful tool for engineering protein function. The process of directed evolution involves iterative rounds of sequence diversification followed by assaying activity of variants and selection. The range of sequence variants and linked activities generated in the course of an evolution are a rich information source for investigating relationships between sequence and function. Key residue positions determining protein function, combinatorial contributors to activity and even potential functional mechanisms have been revealed in directed evolutions. The recent application of high throughput sequencing substantially increases the information that can be retrieved from directed evolution experiments. Combined with computational analysis this additional sequence information has allowed high‐resolution analysis of individual residue contributions to activity. These developments promise to significantly enhance the depth of insight that experimental evolution provides into mechanisms of protein function. 相似文献
68.
69.
Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesity 下载免费PDF全文
70.
Ronald E. Bontrop Marcel G. J. Tilanus Marlies M. A. Mikulski Dienne G. Elferink Annemarie Termijtelen Rene R. P. de Vries Jon J. van Rood Marius J. Giphart 《Immunogenetics》1988,27(1):40-45
HLA-DR molecules were isolated from HLA-DR3, –5, and –w6 positive homozygous B-cell lines by immunoprecipitation with monoclonal antibodies and analyzed by gel electrophoretic techniques. DNA isolated from the same cell lines was digested with the restriction enzyme Taq I and hybridized with a DR beta full-length cDNA probe. We demonstrated that certain DR
I alleles are found in combination with different DR
III alleles as defined by Southern blotting, protein chemistry, a functional assay using purified protein derivative-specific T-cell lines, and, in one case, also alloreactive T-cell reagents. Our results indicate that within the family of HLA-DRw52-associated haplotypes DR beta chain genes may have been transferred from one haplotype to another. The implications of these findings are discussed. 相似文献