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101.
Phenotypic plasticity, i.e., the ability of a genotype to produce various phenotypes in response to changes in the environment, plays an important, although poorly understood and often underestimated, role in evolution. Both adaptive and nonadaptive phenotypic plasticity modulate the strength and direction of selection acting on a population and can, depending on conditions, either accelerate or inhibit adaptation, divergence, and speciation. Phenotypic plasticity also affects the direction of evolutionary change, which can either coincide with the direction of plastic changes (genetic assimilation) or be the opposite (genetic compensation). A special case of phenotypic plasticity is phenotypic change of the host caused by changes in its symbiotic microbiota. In the current review, we discuss the main forms of phenotypic plasticity and the current data on their impact on the rate and direction of evolutionary change. Special attention is paid to the results of recent experimental work, including the long-term evolutionary experiment on Drosophila melanogaster, which is being held at the Department of Evolutionary Biology, School of Biology, Moscow State University. 相似文献
102.
We investigated the prevalence and phenotypic variation of Candida species in oral lichen planus (OLP) and the therapeutic implications of our findings. Eighty patients with clinically and histopathologically confirmed cases of OLP (64 non-erosive, 16 erosive) and a control group of 80 healthy individuals with no predisposing factors for oral candidiasis were examined for evidence of Candida infection. Oral swabs and smears were obtained for cytology and culture. Identification, speciation and antifungal susceptibility tests of Candida isolates were performed using an automated microbial identification system. Fifty percent of erosive OLP cases, 28% of non-erosive cases and none of the controls showed evidence of Candida. Candida albicans was found predominantly in non-erosive OLP, while other Candida species were predominate in erosive OLP. Non-Candida albicans isolates (C. glabrata, C. krusei) were resistant to the commonly used antifungals, clotrimazole and fluconazole. Candida infection is common in cases of OLP. We recommend antifungal sensitivity testing prior to antifungal therapy for the erosive form of OLP. 相似文献
103.
Vera A. Borzova Kira A. Markossian Natalia A. Chebotareva Sergey Yu. Kleymenov Nikolay B. Poliansky Konstantin O. Muranov Vita A. Stein-Margolina Vladimir V. Shubin Denis I. Markov Boris I. Kurganov 《PloS one》2016,11(4)
Thermal aggregation of bovine serum albumin (BSA) has been studied using dynamic light scattering, asymmetric flow field-flow fractionation and analytical ultracentrifugation. The studies were carried out at fixed temperatures (60°C, 65°C, 70°C and 80°C) in 0.1 M phosphate buffer, pH 7.0, at BSA concentration of 1 mg/ml. Thermal denaturation of the protein was studied by differential scanning calorimetry. Analysis of the experimental data shows that at 65°C the stage of protein unfolding and individual stages of protein aggregation are markedly separated in time. This circumstance allowed us to propose the following mechanism of thermal aggregation of BSA. Protein unfolding results in the formation of two forms of the non-native protein with different propensity to aggregation. One of the forms (highly reactive unfolded form, Uhr) is characterized by a high rate of aggregation. Aggregation of Uhr leads to the formation of primary aggregates with the hydrodynamic radius (Rh,1) of 10.3 nm. The second form (low reactive unfolded form, Ulr) participates in the aggregation process by its attachment to the primary aggregates produced by the Uhr form and possesses ability for self-aggregation with formation of stable small-sized aggregates (Ast). At complete exhaustion of Ulr, secondary aggregates with the hydrodynamic radius (Rh,2) of 12.8 nm are formed. At 60°C the rates of unfolding and aggregation are commensurate, at 70°C the rates of formation of the primary and secondary aggregates are commensurate, at 80°C the registration of the initial stages of aggregation is complicated by formation of large-sized aggregates. 相似文献
104.
Nuclear DNA isolated from aSaccharomyces cerevisiae
ρ mutant was studied for the presence of repetitive sequences. A main-band DNA preparation free of rRNA genes and 2-μm plasmid
DNA was prepared by density gradient centrifugation in Cs2SO4−Ag+. A fast renaturing fraction was obtained from this mainband DNA by 3 cycles of reassociation at a low C0t value (0.2). This fraction reassociated 10 times faster than the bulk of the main-band DNA. Its sequences comprised about
3% of the genome and showed a considerable heterogeneity in respect to repetitiveness. The relationship of this fraction to
the repetitive transposable elements recently found in yeast cells is discussed. 相似文献
105.
A group of antigenically distinct proteins characteristic for the tissue complex of the vascular cylinders was found in maize (Zea mays L.) seedlings using an immunofiltration technique. Specific stelar antigens present in the fully developed stele (vascular cylinder) of the primary root were also found in steles extracted from adventitious roots and from the mesocotyl but were absent, within the limits of sensitivity of the immunodiffusion tests employed, in root cortex and epidermis. Some of the stelar antigens were also evident in the meristem of the primary root and were present in traces in the scutellum, the mesocotyl node, and the primary leaves plus coleoptile. The specific stelar antigens could be traced in 13- and 15-day-old developing embryos and were definitely expressed by the 21 st day after pollination. Several stelar-specific antigens were found in embryo-derived callus tissues and in stem-derived cells maintained in serial suspension culture. Higher resolution of the stelar antigens by a modified technique of crossed immunoelectrophoresis was used to demonstrate several minor stelar antigens that were presumably characteristic exclusively of the completely differentiated stele. This technique along with sequential immunoprecipitation of labelled proteins provided a semiquantitative estimate of the specific stelar antigens in the meristem and the stele of the primary root, and in suspension-cultured cells which were devoid of noticeable signs of vascular differentiation. 相似文献
106.
Fermentation of yoghurt and acidified milks containing aflatoxin B1 (AB1) were studied. AB1 added to milk before fermentation at concentrations of 600, 1000 and 1400 g/kg was reduced in yoghurts (pH 4.0) by 97, 91 and 90%, respectively. Coagulation time was approximately the same as in the controls. Streptococci had longer chains than those in the controls. The main decrease of AB1 occurred during the milk fermentation. A decrease of AB1 (conc. 1000 g/kg) in milks acidified with citric, lactic and acetic acids (pH 4.0) was 90, 84 and 73%, respectively. 相似文献
107.
N I Berzin' Iu G Markov A R Val'dman 《Biulleten' eksperimental'no? biologii i meditsiny》1989,108(7):37-40
Vitamin A increase the accumulation and transport of zinc in the chick ileal mucosa. Regulating effect of this vitamin on the mentioned processes was already observed in the initial stage zinc uptake in the intestinal mucosa--its storage in the mucus layer. Stimulation of zinc absorption by vitamin A can be related to a specific carrier-vitamin A-dependent zinc-binding protein. Presence of zinc in the smooth endoplasmic reticulum of the enterocyte apical zone suggests the role of this organelle in the cation homeostasis in the cell. 相似文献
108.
Microbiology - Drosophila melanogaster fruit flies are an important model for studying the multifaceted interactions between a multicellular organism and its microbiome. The nature of these... 相似文献
109.
110.
Gema Valera Daniil A. Markov Kayvan Bijari Owen Randlett Amir Asgharsharghi Jean-Pierre Baudoin Giorgio A. Ascoli Ruben Portugues Hernán López-Schier 《Current biology : CB》2021,31(7):1463-1475.e6
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