全文获取类型
收费全文 | 2368篇 |
免费 | 231篇 |
国内免费 | 1篇 |
专业分类
2600篇 |
出版年
2022年 | 16篇 |
2021年 | 37篇 |
2020年 | 22篇 |
2019年 | 31篇 |
2018年 | 27篇 |
2017年 | 33篇 |
2016年 | 69篇 |
2015年 | 94篇 |
2014年 | 88篇 |
2013年 | 119篇 |
2012年 | 197篇 |
2011年 | 175篇 |
2010年 | 118篇 |
2009年 | 77篇 |
2008年 | 178篇 |
2007年 | 157篇 |
2006年 | 164篇 |
2005年 | 139篇 |
2004年 | 127篇 |
2003年 | 122篇 |
2002年 | 163篇 |
2001年 | 35篇 |
2000年 | 23篇 |
1999年 | 33篇 |
1998年 | 42篇 |
1997年 | 13篇 |
1996年 | 16篇 |
1995年 | 19篇 |
1994年 | 21篇 |
1993年 | 15篇 |
1992年 | 24篇 |
1991年 | 18篇 |
1990年 | 11篇 |
1989年 | 10篇 |
1988年 | 7篇 |
1987年 | 13篇 |
1986年 | 8篇 |
1985年 | 9篇 |
1984年 | 10篇 |
1983年 | 8篇 |
1982年 | 9篇 |
1981年 | 10篇 |
1980年 | 13篇 |
1979年 | 10篇 |
1976年 | 8篇 |
1975年 | 11篇 |
1973年 | 10篇 |
1972年 | 5篇 |
1971年 | 7篇 |
1969年 | 5篇 |
排序方式: 共有2600条查询结果,搜索用时 15 毫秒
101.
102.
Characterization of estrous cycles and pregnancy in Somali wild asses (Equus africanus somaliensis) through fecal hormone analyses 下载免费PDF全文
Corinne P. Kozlowski Helen L. Clawitter Tim Thier Martha T. Fischer Cheryl S. Asa 《Zoo biology》2018,37(1):35-39
Although reproduction in the domestic horse has been well described, less is known about reproduction in wild equids. This study describes endocrine patterns associated with estrous cycles and pregnancy for Somali wild asses (Equus africanus somaliensis), an endangered African equid. Fecal samples were collected three times per week for more than 2 years from five female Somali wild asses at the Saint Louis Zoo; progestagen and estrogen metabolites were quantified using commercially available immunoassays. Progestagen analysis indicated that cycle lengths were 27.2 ± 1.2 days and females cycled throughout the year. Progestagen levels during early pregnancy were low and not sustained above baseline until approximately 40 weeks prior to partition. Concentrations increased markedly around 16 weeks prior to delivery and peaked 2–3 weeks before birth. Fecal estrogen levels also increased significantly starting 40–45 weeks before parturition and reached their maximal value approximately 20 weeks prior to birth. Neither foal heat nor lactational suppression of estrus was observed, and females cycled within 45 days after delivery. These data are the first to describe the reproductive physiology of Somali wild asses. As the species faces increasing threats in the wild, this information may support conservation efforts by assisting with ex situ breeding programs. 相似文献
103.
104.
Cheryl D. Helgason Lianfa Shi Arnold H. Greenberg Yufang Shi Peter Bromley Thomas G. Cotter Douglas R. Green R. Chris Bleackley 《Experimental cell research》1993,206(2)
Cytotoxic T lymphocyte (CTL)-mediated lysis is accompanied by fragmentation of target cell DNA into an oligonucleosome ladder, a hallmark of apoptosis. Is this a fortuitous coincidence, or could CTL be inducing lysis by activation of the suicide signal? In this report we demonstrate that CTL-mediated target cell death can be blocked with the drug aurintricarboxylic acid (ATA). The abrogation of death correlates with the inhibition of DNA fragmentation. While ATA prevented DNA fragmentation, it failed to significantly alter protein, RNA, or DNA synthesis in the cell lines over the dose range used. In addition, there was no inhibition of cell-cell interaction or granule exocytosis during CTL-mediated killing. ATA also significantly inhibited the cytolysis and DNA fragmentation mediated by isolated cytolytic granules, as well as the granular protein fragmentin. We developed an assay in which target cells could be separated from CTL after binding and programming for lysis. Once they had received the "kiss of death," target cells could be rescued from lysis (as indicated by inhibition of DNA fragmentation and increased target cell viability) by treatment with ATA. These results suggest that ATA blocks target cell death by inhibition of DNA fragmentation, and further, that chromatin degradation is a cause rather than a result of cell death in CTL-mediated lysis. 相似文献
105.
106.
Dessus-Babus S Moore CG Whittimore JD Wyrick PB 《Microbes and infection / Institut Pasteur》2008,10(5):563-570
A common model for studying Chlamydia trachomatis and growing chlamydial stocks uses Lymphogranuloma venereum serovar L2 and non-polarized HeLa cells. However, recent publications indicate that the growth rate and progeny yields can vary considerably for a particular strain depending on the cell line/type used, and seem to be partially related to cell tropism. In the present study, the growth of invasive serovar L2 was compared in endometrial HEC-1B and endocervical HeLa cells polarized on collagen-coated microcarrier beads, as well as in HeLa cells grown in tissue culture flasks. Microscopy analysis revealed no difference in chlamydial attachment/entry patterns or in inclusion development throughout the developmental cycle between cell lines. Very comparable growth curves in both cell lines were also found using real-time PCR analysis, with increases in chlamydial DNA content of 400-500-fold between 2 and 36 h post-inoculation. Similar progeny yields with comparable infectivity were recovered from HEC-1B and HeLa cell bead cultures, and no difference in chlamydial growth was found in polarized vs. non-polarized HeLa cells. In conclusion, unlike other C. trachomatis strains such as urogenital serovar E, invasive serovar L2 grows equally well in physiologically different endometrial and endocervical environments, regardless of the host cell polarization state. 相似文献
107.
Fiona McQuaid Rachel Mulholland Yuma Sangpang Rai Utkarsh Agrawal Helen Bedford J. Claire Cameron Cheryl Gibbons Partho Roy Aziz Sheikh Ting Shi Colin R. Simpson Judith Tait Elise Tessier Steve Turner Jaime Villacampa Ortega Joanne White Rachael Wood 《PLoS medicine》2022,19(2)
BackgroundIn 2020, the SARS-CoV-2 (COVID-19) pandemic and lockdown control measures threatened to disrupt routine childhood immunisation programmes with early reports suggesting uptake would fall. In response, public health bodies in Scotland and England collected national data for childhood immunisations on a weekly or monthly basis to allow for rapid analysis of trends. The aim of this study was to use these data to assess the impact of different phases of the pandemic on infant and preschool immunisation uptake rates.Methods and findingsWe conducted an observational study using routinely collected data for the year prior to the pandemic (2019) and immediately before (22 January to March 2020), during (23 March to 26 July), and after (27 July to 4 October) the first UK “lockdown”. Data were obtained for Scotland from the Public Health Scotland “COVID19 wider impacts on the health care system” dashboard and for England from ImmForm.Five vaccinations delivered at different ages were evaluated; 3 doses of “6-in-1” diphtheria, tetanus, pertussis, polio, Haemophilus influenzae type b, and hepatitis B vaccine (DTaP/IPV/Hib/HepB) and 2 doses of measles, mumps, and rubella (MMR) vaccine. This represented 439,754 invitations to be vaccinated in Scotland and 4.1 million for England. Uptake during the 2020 periods was compared to the previous year (2019) using binary logistic regression analysis. For Scotland, uptake within 4 weeks of a child becoming eligible by age was analysed along with geographical region and indices of deprivation. For Scotland and England, we assessed whether immunisations were up-to-date at approximately 6 months (all doses 6-in-1) and 16 to 18 months (first MMR) of age.We found that uptake within 4 weeks of eligibility in Scotland for all the 5 vaccines was higher during lockdown than in 2019. Differences ranged from 1.3% for first dose 6-in-1 vaccine (95.3 versus 94%, odds ratio [OR] compared to 2019 1.28, 95% confidence intervals [CIs] 1.18 to 1.39) to 14.3% for second MMR dose (66.1 versus 51.8%, OR compared to 2019 1.8, 95% CI 1.74 to 1.87). Significant increases in uptake were seen across all deprivation levels.In England, fewer children due to receive their immunisations during the lockdown period were up to date at 6 months (6-in-1) or 18 months (first dose MMR). The fall in percentage uptake ranged from 0.5% for first 6-in-1 (95.8 versus 96.3%, OR compared to 2019 0.89, 95% CI 0.86– to 0.91) to 2.1% for third 6-in-1 (86.6 versus 88.7%, OR compared to 2019 0.82, 95% CI 0.81 to 0.83).The use of routinely collected data used in this study was a limiting factor as detailed information on potential confounding factors were not available and we were unable to eliminate the possibility of seasonal trends in immunisation uptake.ConclusionsIn this study, we observed that the national lockdown in Scotland was associated with an increase in timely childhood immunisation uptake; however, in England, uptake fell slightly. Reasons for the improved uptake in Scotland may include active measures taken to promote immunisation at local and national levels during this period and should be explored further. Promoting immunisation uptake and addressing potential vaccine hesitancy is particularly important given the ongoing pandemic and COVID-19 vaccination campaigns.Fiona McQuaid and colleagues assess the uptake of infant and pre-school immunisations in Scotland and England during the COVID-19 pandemic. 相似文献
108.
Alterations of plasma lipids in mice via adenoviral-mediated hepatic overexpression of human ABCA1 总被引:3,自引:0,他引:3
Wellington CL Brunham LR Zhou S Singaraja RR Visscher H Gelfer A Ross C James E Liu G Huber MT Yang YZ Parks RJ Groen A Fruchart-Najib J Hayden MR 《Journal of lipid research》2003,44(8):1470-1480
ATP binding cassette transporter A1 (ABCA1) is a widely expressed lipid transporter essential for the generation of HDL. ABCA1 is particularly abundant in the liver, suggesting that the liver may play a major role in HDL homeostasis. To determine how hepatic ABCA1 affects plasma HDL cholesterol levels, we treated mice with an adenovirus (Ad)-expressing human ABCA1 under the control of the cytomegalovirus promoter. Treated mice showed a dose-dependent increase in hepatic ABCA1 protein, ranging from 1.2-fold to 8.3-fold using doses from 5 x 108 to 1.5 x 109 pfu, with maximal expression observed on Day 3 posttreatment. A selective increase in HDL cholesterol occurred at Day 3 in mice treated with 5 x 108 pfu Ad-ABCA1, but higher doses did not further elevate HDL cholesterol levels. In contrast, total cholesterol, triglycerides, phospholipids, non-HDL cholesterol, and apolipoprotein B levels all increased in a dose-dependent manner, suggesting that excessive overexpression of hepatic ABCA1 in the absence of its normal regulatory sequences altered total lipid homeostasis. At comparable expression levels, bacterial artificial chromosome transgenic mice, which express ABCA1 under the control of its endogenous regulatory sequences, showed a greater and more specific increase in HDL cholesterol than Ad-ABCA1-treated mice. Our results suggest that appropriate regulation of ABCA1 is critical for a selective increase in HDL cholesterol levels. 相似文献
109.
Longevity and population age structure of the arroyo southwestern toad (Anaxyrus californicus) with drought implications 下载免费PDF全文
Robert N. Fisher Cheryl S. Brehme Stacie A. Hathaway Tim E. Hovey Manna L. Warburton Drew C. Stokes 《Ecology and evolution》2018,8(12):6124-6132
The arroyo southwestern toad is a specialized and federally endangered amphibian endemic to the coastal plains and mountains of central and southern California and northwestern Baja California. It is largely unknown how long these toads live in natural systems, how their population demographics vary across occupied drainages, and how hydrology affects age structure. We used skeletochronology to estimate the ages of adult arroyo toads in seven occupied drainages with varying surface water hydrology in southern California. We processed 179 adult toads with age estimates between 1 and 6 years. Comparisons between skeletochronological ages and known ages of PIT tagged toads showed that skeletochronology likely underestimated toad age by up to 2 years, indicating they may live to 7 or 8 years, but nonetheless major patterns were evident. Arroyo toads showed sexual size dimorphism with adult females reaching a maximum size of 12 mm greater than males. Population age structure varied among the sites. Age structure at sites with seasonally predictable surface water was biased toward younger individuals, which indicated stable recruitment for these populations. Age structures at the ephemeral sites were biased toward older individuals with cohorts roughly corresponding to higher rainfall years. These populations are driven by surface water availability, a stochastic process, and thus more unstable. Based on our estimates of toad ages, climate predictions of extreme and prolonged drought events could mean that the number of consecutive dry years could surpass the maximum life span of toads making them vulnerable to extirpation, especially in ephemeral freshwater systems. Understanding the relationship between population demographics and hydrology is essential for predicting species resilience to projected changes in weather and rainfall patterns. The arroyo toad serves as a model for understanding potential responses to climatic and hydrologic changes in Mediterranean stream systems. We recommend development of adaptive management strategies to address these threats. 相似文献
110.
Observations of the potto (Perodicticus potto),a nocturnal prosimian primate,indicated a limited behavioral repertoire and prompted renovation of their exhibit at the Frankin Park Zoo (Boston, MA). We
used the natural history of this species to direct the exhibit modifications, which used only nonsynthetic items. We added
live plants, soil, bamboo, shelf fungi, grapevines, a hollow tree, and a log containing an insect dispenser to the exhibit
at little expense. They provided new textures, odors, pathways, stimulation and cover for this secretive species. The changes
produced a heightened aesthetic appeal to the nocturnal exhibit and improved its educational value by representing the animals’
habitat more accurately. The exhibit modifications also resulted in a significant increase in activity, an expansion of the
pottos’ behavioral repertoire,the emergence of sexual behaviors, and an increased visibility to the public. 相似文献