全文获取类型
收费全文 | 486篇 |
免费 | 85篇 |
出版年
2021年 | 6篇 |
2020年 | 4篇 |
2019年 | 3篇 |
2018年 | 5篇 |
2016年 | 6篇 |
2015年 | 13篇 |
2014年 | 9篇 |
2013年 | 12篇 |
2012年 | 12篇 |
2011年 | 12篇 |
2010年 | 5篇 |
2009年 | 14篇 |
2008年 | 16篇 |
2007年 | 15篇 |
2006年 | 11篇 |
2005年 | 10篇 |
2004年 | 12篇 |
2003年 | 21篇 |
2002年 | 25篇 |
2001年 | 19篇 |
2000年 | 33篇 |
1999年 | 21篇 |
1998年 | 14篇 |
1997年 | 13篇 |
1996年 | 10篇 |
1995年 | 14篇 |
1994年 | 5篇 |
1993年 | 8篇 |
1992年 | 14篇 |
1991年 | 14篇 |
1990年 | 23篇 |
1989年 | 8篇 |
1988年 | 9篇 |
1987年 | 13篇 |
1986年 | 6篇 |
1985年 | 12篇 |
1984年 | 13篇 |
1983年 | 8篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 16篇 |
1979年 | 6篇 |
1978年 | 15篇 |
1977年 | 9篇 |
1976年 | 3篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1942年 | 2篇 |
1892年 | 2篇 |
排序方式: 共有571条查询结果,搜索用时 859 毫秒
81.
P.S. Cocks I.S. Fraser R. Markham M. Robinson R.P. Shearman 《Prostaglandins & other lipid mediators》1980,20(3):493-502
Peripheral plasma concentrations of 13, 14-dihydro-15-keto prostaglandin F2α (PGFM) were measured in seven women having elective cervical encerclage in an otherwise normal pregnancy. Three of seven patients had a statistically significant elevation of PGFM levels at 60 minutes after commencement of surgery. On the basis that the majority of patients had no significant rise and that there was no objective evidence of uterine contractions post-operatively, the routine administration of prostaglandin inhibitory agents to patients having cervical encerclage cannot be recommended. 相似文献
82.
The CD spectra of cUpUp, cCpCp, and cGpGp derived from DCC-catalyzed polymerization of the relevant protected ribonucleoside 3′-phosphates are described. Similar studies on Up, U > p, and cUp, as well as cUpUpUp and cUpUpUpUp, are presented. The spectral properties of the cyclic oligomers are compared with those of the corresponding linear oligomers with terminal 3′-phosphates so as to demonstrate that disruption of normal right-handed base stacking is considerable in these RNA loops. 相似文献
83.
A wide variety of novel flavone 5-O-glucosides has been isolated from Lycopodium scariosum. The predominance of tricin derivatives in this species is not in accord with earlier proposed chemotaxonomic definitions of the sub-groups within the classical genus Lycopodium L. cernuum is the first lycopod found to contain flavone C-glycosides and its suggested taxonomic separation from other members of the group is thus substantiated. Flavonoid glycosides appear to offer more definitive chemotaxonomic characters than do the previously used aglycones and modification of the chemical definitions of the sub-groups in these terms is necessary. 相似文献
84.
The advent of the polymerase chain reaction has stimulated the development of a number of rapid methods for characterizing human genes. In addition, the unprecedented level of sensitivity offered by some of these methods may prove to be of great value in the detection of minority cell populations. Over the past year, technical developments have been made in this area. 相似文献
85.
S-Adenosylmethionine synthetase from Escherichia coli 总被引:16,自引:0,他引:16
G D Markham E W Hafner C W Tabor H Tabor 《The Journal of biological chemistry》1980,255(19):9082-9092
Adenosylmethionine (AdoMet) synthetase has been purified to homogeneity from Escherichia coli. For this purification, a strain of E. coli which was derepressed for AdoMet synthetase and which harbors a plasmid containing the structural gene for AdoMet synthetase was constructed. This strain produces 80-fold more AdoMet synthetase than a wild type E. coli. AdoMet synthetase has a molecular weight of 180,000 and is composed of four identical subunits. In addition to the synthetase reaction, the purified enzyme catalyzes a tripolyphosphatase reaction that is stimulated by AdoMet. Both enzymatic activities require a divalent metal ion and are markedly stimulated by certain monovalent cations. AdoMet synthesis also takes place if adenyl-5'yl imidodiphosphate (AMP-PNP) is substituted for ATP. The imidotriphosphate (PPNP) formed is not hydrolyzed, permitting dissociation of AdoMet formation from tripolyphosphate cleavage. An enzyme complex is formed which contains one equivalent (per subunit) of adenosylmethionine, monovalent cation, imidotriphosphate, and presumably divalent cation(s). The rate of product dissociation from this complex is 3 orders of magnitude slower than the rate of AdoMet formation from ATP. Studies with the phosphorothioate derivatives of ATP (ATP alpha S and ATP beta S) in the presence of Mg2+, Mn2+, or Co2+ indicate that a divalent ion is bound to the nucleotide during the reaction and provide information on the stereochemistry of the metal-nucleotide binding site. 相似文献
86.
L M Moynihan S E Bundey D Heath E L Jones D P McHale R F Mueller A F Markham N J Lench 《American journal of human genetics》1998,62(5):1123-1128
We describe a highly consanguineous family, originating from Pakistan, displaying histiocytosis, joint contractures, and sensorineural deafness. The form of histiocytosis exhibited by this family does not fit readily into any of the recognized classes of this disease. It appears to represent a novel form of familial histiocytosis demonstrating autosomal recessive inheritance. Using autozygosity mapping, we have identified a homozygous region of approximately 1 cM at chromosome 11q25, in affected individuals. A maximum two-point LOD score of 3.42 (recombination fraction straight theta = .00) was obtained with marker D11S968. This is the first genetic locus to be described that is involved in the molecular pathogenesis of histiocytosis. 相似文献
87.
Rai Ajit K. Srivastava Joseph A. Cornicelli Bruce Markham Charles L. Bisgaier 《Molecular and cellular biochemistry》2018,438(1-2):167-174
The marked clinical and genetic heterogeneity seen in hypertrophic (HCM) and dilated cardiomyopathies (DCM) suggests involvement of disease modifiers and environmental factors in the pathophysiology of these diseases. In the current study, we examined association of single nucleotide polymorphisms (SNPs) of three candidate genes, ACE2 (rs6632677), TNNI3K (rs49812611) and CALM3 (rs13477425) with clinical phenotypes of HCM and DCM patients of North Indian ethnicity. Prevalence of ACE2 (7160726 C>G) variant genotypes (CG and GG) was significantly higher in DCM subjects as compared to controls. Prevalence of TNNI3K (3784 C>T) and CALM3 (?34T>A) variant homozygous genotype were significantly higher in HCM and DCM subjects as compared to controls. DCM patients with CT genotype showed significant decrease in LVEF as compared to CC genotype (p < 0.03). There was significant gene–gene interaction between these SNPs and three-way SNP combination of ACE2 C>G, TNN13K C>T, CALM3 A>T gene variants and was associated with high risk of HCM and DCM. Presence of ACE2 (7160726 C>G) and CALM3 (?34T>A) variant genotypes in HCM Patients with mutations (sarcomeric or non sarcomeric genes) was associated with increased mean septal thickness, further suggesting a role of these gene variants in modifying disease phenotype. Our results suggest that ACE2, TNNI3K and CALM3 polymorphisms are associated with increased risk of HCM and DCM and may act as disease modifiers of these diseases. 相似文献
88.
D. Scholz C. Borgemeister W.G. Meikle R.H. Markham H.-M. Poehling 《Entomologia Experimentalis et Applicata》1997,83(1):53-61
Delta traps baited with maize cobs, which were infested each with one male Prostephanus truncatus (Horn) (Col.: Bostrichidae), were distributed in southern Benin and collected after one, two, three and four weeks. The numbers of P. truncatus caught during the different trapping periods were not significantly different. Sixty-four percent of the trapped P. truncatus were females. Females attracted during the one-week trapping period produced a mean of 6.9 progeny during the seven days. The sex ratio of the progeny was 1:1. Trap catches with the infested cobs were on average 13 times lower than catches with 2 mg of the artificial pheromone. Estimation of P. truncatus densities in a maize store at the beginning of the storage period (based on laboratory data) revealed that small initial numbers of P. truncatus, possibly attracted by a single male, sufficed to initiate high infestation rates later in the storage season. 相似文献
89.
L-Lactate dehydrogenase (L-LDH, E.C. 1.1.1.27) is encoded by two or three
loci in all vertebrates examined, with the exception of lampreys, which
have a single LDH locus. Biochemical characterizations of LDH proteins have
suggested that a gene duplication early in vertebrate evolution gave rise
to Ldh-A and Ldh-B and that an additional locus, Ldh-C arose in a number of
lineages more recently. Although some phylogenetic studies of LDH protein
sequences have supported this pattern of gene duplication, others have
contradicted it. In particular, a number of studies have suggested that
Ldh-C represents the earliest divergence among vertebrate LDHs and that it
may have diverged from the other loci well before the origin of
vertebrates. Such hypotheses make explicit statements about the
relationship of vertebrate and invertebrate LDHs, but to date, no closely
related invertebrate LDH sequences have been available for comparison. We
have attempted to provide further data on the timing of gene duplications
leading to multiple vertebrate LDHs by determining the cDNA sequence of the
LDH of the tunicate Styela plicata. Phylogenetic analyses of this and other
LDH sequences provide strong support for the duplications giving rise to
multiple vertebrate LDHs having occurred after vertebrates diverged from
tunicates. The timing of these LDH duplications is consistent with data
from a number of other gene families suggesting widespread gene duplication
near the origin of vertebrates. With respect to the relationships among
vertebrate LDHs, our data are not consistent with previous claims that
Ldh-C represented the earliest divergence. However, the precise
relationships among some of the main lineages of vertebrate LDHs were not
resolved in our analyses.
相似文献
90.
J. G. Sánchez D. J. Speare R. J. F. Markham S. R. M. Jones 《Journal of fish biology》2001,59(2):427-441
A Salvelinus -infecting variant of Loma salmonae , derived from naturally-infected Chinook salmon Oncorhynchus tshawytscha by serial passage through brook trout Salvelinus fontinalis , has been isolated and amplified. Loma salmonae SV ( Salvelinus -variant) has a high preference for species of Salvelinus (brook trout and Arctic charr S. alpinus ) and low virulence and preference for species of Oncorhynchus (rainbow trout O. mykiss , Chinook salmon, cohoSalmon O. kisutch ) or Salmo (Atlantic salmon Salmo salar ). Although this variant of L. salmonae was different from the original, the differences do not justify describing it as a new species, although definitive determination is pending. 相似文献