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731.
Edyta Podmokła Anna Dubiec Szymon M. Drobniak Aneta Arct Lars Gustafsson Mariusz Cichoń 《Journal of avian biology》2014,45(3):219-224
Haemosporidians causing avian malaria are very common parasites among bird species. Their negative effects have been repeatedly reported in terms of deterioration in survival prospects or reproductive success. However, a positive association between blood parasites and avian fitness has also been reported. Here, we studied a relationship between presence of malaria parasites and reproductive performance of the host, a hole‐breeding passerine – the blue tit Cyanistes caeruleus. Since the malaria parasites might affect their hosts differently depending on environmental conditions, we performed brood size manipulation experiment to differentiate parental reproductive effort and study the potential interaction between infection status and brood rearing conditions on reproductive performance. We found individuals infected with malaria parasites to breed later in the season in comparison with uninfected birds, but no differences were detected in clutch size. Interestingly, infected parents produced heavier and larger offspring with stronger reaction to phytohemagglutinin. More importantly, we found a significant interaction between infection status and brood size manipulation in offspring tarsus length and reaction to phytohemagglutinin: presence of parasites had stronger positive effect among birds caring for experimentally enlarged broods. Our results might be interpreted either in the light of the parasite‐mediated selection or terminal investment hypothesis. 相似文献
732.
Marta Szal Mirosława Kupryjanowicz Mariusz Wyczółkowski Wojciech Tylmann 《Vegetation History and Archaeobotany》2014,23(4):419-437
Pollen, non-pollen palynomorphs, charcoal and geochemical analyses of sediments from Lake Sal?t (NE Poland) were used to reconstruct vegetation changes related to the activity of the West Balt tribes during the Iron Age, in the period between the second half of the 7th century bc and the beginning of the 10th century ad. We distinguished five phases of human impact on environment. Woodland clearing around the studied lake started at the end of the 7th century bc. The most characteristic feature of this area during the whole Iron Age was a very high representation of semi-natural Betula woodlands, which was probably linked to a shifting agriculture. This type of land use lasted for over 1,500 years, until the second half of the 9th century ad. The greatest reduction in Betula woodlands took place between cal. years 650 and 450 bc. Its regeneration took place after ca. ad 830 when human activity decreased. 相似文献
733.
The relationship between climate data and tick questing activity is crucial for estimation of the spatial and temporal distribution of the risk of ticks and tick‐borne diseases. This study establishes correlations between selected meteorological variables provided by the Weather Research and Forecasting model (WRF) and the questing activity of Ixodes ricinus nymphs and adults on a regional scale across Lower Silesia, Poland. Application of Generalized Linear Mixed Models (GLMM), built separately for adults and nymphs, showed that solar radiation, air temperature, and saturation deficit appeared to be the meteorological variables of prime importance, whereas the wind speed was less important. However, the effect of meteorological parameters was different for adults and nymphs. The adults are also more influenced by forest cover and the percentage of forest type if compared to nymphs. The WRF model providing meteorological variables separately for each location and day of tick sampling can be useful in studies of questing activity of ticks on a regional scale. 相似文献
734.
Microtubules are key structural elements that, among numerous biological functions, maintain the cytoskeleton of the cell and have a major role in cell division, which makes them important cancer chemotherapy targets. Understanding the energy balance that brings tubulin dimers, the building blocks of microtubules, together to form a microtubule is especially important for revealing the mechanism of their dynamic instability. Several studies have been conducted to estimate various contributions to the free energy of microtubule formation. However, the hydrogen-bond contribution was not studied before as a separate component. In this work, we use concepts such as the quantum theory of atoms in molecules to estimate the per-residue strength of hydrogen bonds contributing to the overall stability that brings subunits together in pair of tubulin heterodimers, across both the longitudinal and lateral interfaces. Our study shows that hydrogen bonding plays a major role in the stability of tubulin systems. Several residues that are crucial to the binding of vinca alkaloids are shown to be strongly involved in longitudinal microtubule stabilization. This indicates a direct relation between the binding of these agents and the effect on the interfacial hydrogen-bonding network, and explains the mechanism of their action. Lateral contacts showed much higher stability than longitudinal ones (–462 ± 70 vs. –392 ± 59 kJ/mol), which suggests a dramatic lateral stabilization effect of the GTP cap in the β-subunit. The role of the M-loop in lateral stability in absence of taxol was shown to be minor. The B-lattice lateral hydrogen bonds are shown to be comparable in strength to the A-lattice ones (−462 ± 70 vs. –472 ± 46 kJ/mol). These findings establish the importance of hydrogen bonds to the stability of tubulin systems. 相似文献
735.
Marleen Forkink Ganesh R. Manjeri Dania C. Liemburg-Apers Esther Nibbeling Maxime Blanchard Aleksandra Wojtala Jan A.M. Smeitink Mariusz R. Wieckowski Peter H.G.M. Willems Werner J.H. Koopman 《BBA》2014
The mitochondrial oxidative phosphorylation (OXPHOS) system consists of four electron transport chain (ETC) complexes (CI–CIV) and the FoF1-ATP synthase (CV), which sustain ATP generation via chemiosmotic coupling. The latter requires an inward-directed proton-motive force (PMF) across the mitochondrial inner membrane (MIM) consisting of a proton (ΔpH) and electrical charge (Δψ) gradient. CI actively participates in sustaining these gradients via trans-MIM proton pumping. Enigmatically, at the cellular level genetic or inhibitor-induced CI dysfunction has been associated with Δψ depolarization or hyperpolarization. The cellular mechanism of the latter is still incompletely understood. Here we demonstrate that chronic (24 h) CI inhibition in HEK293 cells induces a proton-based Δψ hyperpolarization in HEK293 cells without triggering reverse-mode action of CV or the adenine nucleotide translocase (ANT). Hyperpolarization was associated with low levels of CII-driven O2 consumption and prevented by co-inhibition of CII, CIII or CIV activity. In contrast, chronic CIII inhibition triggered CV reverse-mode action and induced Δψ depolarization. CI- and CIII-inhibition similarly reduced free matrix ATP levels and increased the cell's dependence on extracellular glucose to maintain cytosolic free ATP. Our findings support a model in which Δψ hyperpolarization in CI-inhibited cells results from low activity of CII, CIII and CIV, combined with reduced forward action of CV and ANT. 相似文献
736.
Mark A. Brooks Kamil Gewartowski Eirini Mitsiki Juliette Létoquart Roland A. Pache Ysaline Billier Michela Bertero Margot Corréa Mariusz Czarnocki-Cieciura Michal Dadlez Véronique Henriot Noureddine Lazar Lila Delbos Dorothée Lebert Jan Piwowarski Pascal Rochaix Bettina Böttcher Luis Serrano Bertrand Séraphin Herman van Tilbeurgh Andrzej Dziembowski 《Structure (London, England : 1993)》2010,18(9):1075-1082
737.
Teresa Olczak Halina Wójtowicz Justyna Ciuraszkiewicz Mariusz Olczak 《BMC microbiology》2010,10(1):134
Background
Porphyromonas gingivalis is a major etiological agent of chronic periodontitis. The aim of this study was to examine the species specificity, surface exposure, protein expression, immunogenicity, and participation in biofilm formation of the P. gingivalis heme-binding protein HmuY. 相似文献738.
Dymkowska D Szczepanowska J Wieckowski MR Wojtczak L 《Biochimica et biophysica acta》2006,1763(2):152-163
Arachidonic acid and, to a smaller extent, oleic acid at micromolar concentrations decreased the mitochondrial membrane potential within AS-30D rat hepatoma cells cultivated in vitro and increased cell respiration. The uncoupling effect of both fatty acids on cell respiration was partly prevented by cyclosporin A, blocker of the mitochondrial permeability transition pore. Arachidonic acid increased the rate of reactive oxygen species (ROS) production, while oleic acid decreased it. Both fatty acids induced apoptotic cell death of AS-30D cells, accompanied by the release of cytochrome c from mitochondria to the cytosol, activation of caspase-3 and association of proapoptotic Bax protein with mitochondria; arachidonic acid being a more potent inducer than oleic acid. Trolox, a potent antioxidant, prevented ROS increase induced by arachidonic acid and protected the cells against apoptosis produced by this fatty acid. It is concluded that arachidonic and oleic acids induce apoptosis of AS-30D hepatoma cells by the mitochondrial pathway but differ in the mechanism of their action: Arachidonic acid induces apoptosis mainly by stimulating ROS production, whereas oleic acid may contribute to programmed cell death by activation of the mitochondrial permeability transition pore. 相似文献
739.
There are three components of the total evapotranspiration (soil evaporation, interception loss and transpiration) influenced strongly by the vegetation. Hydrological models need information about the temporally varying portions of the components. On a well equipped research station in the east of Germany all these components have been measured under corn, winter wheat and sunflower (Zea mais L., Triticum aestivum L., Helianthus annuus, L.) and calculated with different methods, respectively. The portion of soil evaporation decreased dramatically from 50% at a LAI = 0.5 to 1–5% for dense crops (LAI > 3). It was stated that transpiration and interception loss took place simultaneously. 相似文献
740.
Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma
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Peter Kubatka Sona Uramova Martin Kello Karol Kajo Peter Kruzliak Jan Mojzis Desanka Vybohova Marian Adamkov Karina Jasek Zora Lasabova Pavol Zubor Silvia Fialova Svetlana Dokupilova Peter Solar Martin Pec Katarina Adamicova Jan Danko Mariusz Adamek Dietrich Busselberg 《Journal of cellular and molecular medicine》2017,21(11):2837-2851
It is supposed that plant functional foods, rich in phytochemicals, may potentially have preventive effects in carcinogenesis. In this study, the anticancer effects of cloves in the in vivo and in vitro mammary carcinoma model were assessed. Dried flower buds of cloves (CLOs) were used at two concentrations of 0.1% and 1% through diet during 13 weeks after the application of chemocarcinogen. After autopsy, histopathological and immunohistochemical analyses of rat mammary carcinomas were performed. Moreover, in vitro evaluation using MCF‐7 cells was carried out. Dietary administered CLO caused the dose‐dependent decrease in tumour frequency by 47.5% and 58.5% when compared to control. Analysis of carcinoma cells in animals showed bcl‐2, Ki67, VEGFA, CD24 and CD44 expression decrease and Bax, caspase‐3 and ALDH1 expression increase after high‐dose CLO administration. MDA levels were substantially decreased in rat carcinomas in both CLO groups. The evaluation of histone modifications revealed increase in lysine trimethylations and acetylations (H4K20me3, H4K16ac) in carcinomas after CLO administration. TIMP3 promoter methylation levels of CpG3, CpG4, CpG5 islands were altered in treated cancer cells. An increase in total RASSF1A promoter methylation (three CpG sites) in CLO 1 group was found. In vitro studies showed antiproliferative and pro‐apoptotic effects of CLO extract in MCF‐7 cells (analyses of cytotoxicity, Brdu, cell cycle, annexin V/PI, caspase‐7, Bcl‐2 and mitochondrial membrane potential). This study showed a significant anticancer effect of clove buds in the mammary carcinoma model in vivo and in vitro. 相似文献