全文获取类型
收费全文 | 1287篇 |
免费 | 94篇 |
出版年
2023年 | 8篇 |
2022年 | 19篇 |
2021年 | 32篇 |
2020年 | 15篇 |
2019年 | 33篇 |
2018年 | 23篇 |
2017年 | 29篇 |
2016年 | 40篇 |
2015年 | 76篇 |
2014年 | 71篇 |
2013年 | 71篇 |
2012年 | 109篇 |
2011年 | 90篇 |
2010年 | 60篇 |
2009年 | 23篇 |
2008年 | 55篇 |
2007年 | 57篇 |
2006年 | 58篇 |
2005年 | 43篇 |
2004年 | 37篇 |
2003年 | 45篇 |
2002年 | 37篇 |
2001年 | 15篇 |
2000年 | 19篇 |
1999年 | 19篇 |
1998年 | 13篇 |
1997年 | 14篇 |
1996年 | 15篇 |
1994年 | 7篇 |
1993年 | 13篇 |
1992年 | 13篇 |
1991年 | 17篇 |
1990年 | 19篇 |
1989年 | 12篇 |
1988年 | 8篇 |
1987年 | 11篇 |
1986年 | 6篇 |
1985年 | 8篇 |
1983年 | 8篇 |
1982年 | 6篇 |
1980年 | 6篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1976年 | 5篇 |
1975年 | 13篇 |
1971年 | 10篇 |
1970年 | 7篇 |
1968年 | 8篇 |
1966年 | 6篇 |
1870年 | 4篇 |
排序方式: 共有1381条查询结果,搜索用时 78 毫秒
11.
Ronald Bontrop Marcel Tilanus Marlies Mikulski Marja van Eggermond Annemarie Termijtelen Marius Giphart 《Immunogenetics》1986,23(6):401-405
HLA-DR molecules were isolated from eight different HLA-DR3 homozygous B-cell lines by immunoprecipitation with monoclonal antibodies, and they were subsequently analyzed by two-dimensional gel electrophoresis. We found that HLA-DR3 homozygous B-cell lines of consanguineous origin express two types of HLA-DR molecules. One type of HLA-DR molecule was present in all the cell lines tested, whereas the second DR molecule appears to be polymorphic. DNA isolated from the different HLA-DR3 homozygous cell lines was studied by Southern blot analysis to determine whether any DR restriction fragment length polymorphism could be observed. Polymorphisms detected at both the product and genomic level have been compared to each other, and their relations to the serological (HLA-DR) and cellular (HLA-D and LB-Q1) typing data will be discussed.No reprints available 相似文献
12.
We have examined the accessibility to diethylpyrocarbonate of spinach chloroplast 4.5S ribosomal RNA when free and when it is part of the ribosomal structure. The modifications in free 4.5S RNA were found mostly in single-stranded regions of the secondary structure model proposed in our previous paper (Kumagai, I. et al. (1982) J.B.C. 257, 12924-28): adenines at positions 17, 19, 33, 36, 54, 55, 60, 64, 68, 72, 77, 86 and 87 were identified as the reactive residues. On the other hand, in 4.5S RNA in 70S ribosomes or 50S subunits, adenine 33 was exclusively modified, and its reactivity was much higher than in free 4.5S RNA. This highly accessible A33 of spinach 4.5S RNA is located within a characteristic seven nucleotide sequence, which is found in the 4.5S rRNAs from spinach, tobacco and a fern but deleted in 4.5S RNAs from maize and wheat. 相似文献
13.
A high potential cytochrome c from Chromatium chromatophores 总被引:1,自引:0,他引:1
14.
15.
Marius Peacock J. Munoz George L. Tallent Richard A. Ormsbee 《Journal of bacteriology》1968,95(5):1580-1586
Passive cutaneous anaphylaxis (PCA) was produced in guinea pigs sensitized with guinea pig Coxiella burneti phase I–II antiserum and challenged with dimethylsulfoxide- or trichloroacetic acid-soluble extracts from phase I cells. The PCA reaction could not be induced by whole or mechanically disrupted phase I or phase II C. burneti cells or by extracted cells or extracts of phase II cells. The antibody responsible for PCA was in the 7Sγ1 (fast γ) globulin. Sensitization of the skin by 7Sγ1 antibody could be blocked nonspecifically by 7Sγ1 globulin from normal serum or from phase II antiserum. The 7Sγ2 (slow γ) globulin antibody inhibited the reaction specifically. Some antiserum pools containing high agglutinin and complement-fixing titers to phase I C. burneti cells failed to initiate the PCA reaction, perhaps due to an imbalanced ratio of γ1 to γ2 specific globulins or to an imbalance in the ratio of specific to nonspecific γ1 globulins. Agglutinins to phase I cells were found in both γ1 and γ2 antibody globulins. Complement-fixing antibodies were found in the γ2 globulin fraction. 相似文献
16.
17.
18.
19.
Coagulation and fibrinolysis in acute mountain sickness and beginning pulmonary edema 总被引:2,自引:0,他引:2
Bartsch P.; Haeberli A.; Franciolli M.; Kruithof E. K.; Straub P. W. 《Journal of applied physiology》1989,66(5):2136-2144
To examine whether intravascular coagulation and/or decreased fibrinolysis precedes high-altitude pulmonary edema (HAPE) we examined 25 male mountaineers (median age 40 yr) at low altitude (550 m) and after 6, 18, and 42 h at an altitude of 4,559 m, which was climbed in 24 h. In 14 subjects, 2 of whom showed radiological evidence of HAPE after 42 h, symptoms of acute mountain sickness (AMS) were mild or absent. Eleven subjects suffered from AMS, six of whom developed radiologically documented HAPE after 18 or 42 h. In the absence of AMS there were no significant changes at high altitude, with the exception of a decrease in bleeding time from 246 +/- 18 to 212 +/- 13 (SE) (P less than 0.05). In AMS, partial thromboplastine time decreased from 34.2 +/- 0.8 to 31.1 +/- 0.5 s (P less than 0.001) and factor VIII procoagulant activity and von Willebrand factor antigen were increased by 57 +/- 12 and 70 +/- 13%, respectively (P less than 0.001), whereas there were no significant changes in beta-thromboglobulin (BTG), fibrinopeptide A (FPA), and fibrin fragment B beta 15-42. In subjects with HAPE, BTG, FPA, and B beta 15-42 were normal before and in beginning HAPE. Preceding HAPE, euglobulin clot lysis time declined at high compared with low altitude from 289 +/- 48 to 201 +/- 42 min without venous occlusion (VO) and from 107 +/- 36 to 86 +/- 31 min after VO (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
20.
K Bartsch R Dichmann P Schmitt E Uhlmann A Schulz 《Applied and environmental microbiology》1990,56(1):7-12
We have cloned the gene encoding a 43-kilodalton transaminase from Escherichia coli K-12 with a specificity for L-phosphinothricin [L-homoalanine-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (Hoechst AG). The structural gene was isolated, together with its own promoter, and shown to be localized on a 1.6-kilobase DraI-BamHI fragment. The gene is subject to catabolite repression by glucose; however, repression could be relieved completely when 4-aminobutyrate (GABA) served as the sole nitrogen source. The regulation pattern obtained and a comparison of the restriction map of the initially cloned 15-kilobase SalI fragment with the physical map of the E. coli K-12 genome suggest that the cloned gene is identical with gabT, a locus on the gab gene cluster of E. coli K-12 which codes for the GABA:2-ketoglutartate transaminase (EC 2.6.1.19). A number of expression plasmids carrying the isolated transaminase gene were constructed. With these constructs, the transaminase expression in transformants of E. coli could be increased up to 80-fold compared with that in a wild-type control, and the transaminase constituted up to 20% of the total soluble protein of the bacteria. Thus, the protein crude extracts of the transformants could be used, after a simple heat precipitation step, for the biotechnological production of L-phosphinothricin in an enzyme reactor. 相似文献