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101.
Marinho AN Miranda NC Braz V Ribeiro-Dos-Santos AK de Souza SM 《Memórias do Instituto Oswaldo Cruz》2006,101(Z2):15-23
The present paper discusses mtDNA and taphonomy of human remains from Moa, Beirada, and Zé Espinho sambaquis of Saquarema, state of Rio de Janeiro, Brazil. New human bone dating by 14C-AMS for Moa archeological site (3810+50 BP - GX-31826-AMS) is included. Preservation of microscopic lamellae and DNA is not related to the macroscopic integrity of the bones. Results here suggest that the preservation of amplifiable DNA fragments may have relation to the preservation of the lamellar arrangement as indicated by optical microscopic examination (polarized light). In 13 human bone fragments from Moa, Beirada, and Zé Espinho it was possible to sequence mtDNA from the 3 individuals of Moa, and from 1 of 4 individuals of Beirada, whose bones also show extensive areas with preserved lamellar structures. The 6 human bone fragments of Zé Espinho and 3 of the 4 fragments of Beirada showed extensive destruction of cortical microstructure represented by cavities, intrusive minerals, and agglomerated microscopic bodies of fungi and bacteria; it was not possible to extract mtDNA from these samples. The results support the hypothesis that the preservation of the microscopic osteon organization is a good predictor for DNA preservation. It was also confirmed the C haplogroup antiquity in Brazil. 相似文献
102.
Daniel P. Bezerra José D. B. Marinho Filho Ana Paula N. N. Alves Cláudia Pessoa Manoel O. de Moraes Otília Deusdênia L. Pessoa Maria Conceição M. Torres Edilberto R. Silveira Francisco A. Viana Letícia V. Costa‐Lotufo 《化学与生物多样性》2009,6(8):1224-1231
Croton regelianus Muell. Arg. , popularly known as ‘velame‐de‐cheiro’, is a native plant from the Northeast of Brazil used in folk medicine to treat diseases of different kinds, including malignant tumors. In this study, the in vitro and in vivo antitumor effects of the essential oil from the leaves of C. regelianus and ascaridole, one of the main constituents, were investigated. In vitro, the essential oil and ascaridole displayed cytotoxicity, showing IC50 values in the range of 22.2 to 48.0 μg/ml in HL‐60 and SF‐295 cell lines for the essential oil, and 6.3 to 18.4 μg/ml in HL‐60 and HCT‐8 cells lines for ascaridole, respectively. The in vivo study, using sarcoma 180 as a tumor model, demonstrated inhibition rates of 28.1 and 31.8% for essential oil, at the 50 and 100 mg/kg, while ascaridole inhibition rates were 33.9% at 10 mg/kg and 33.3% at 20‐mg/kg doses. Histopathological examination showed that the organs were only weakly affected by the treatment. In conclusion, ascaridole has an interesting antitumor activity in sarcoma 180 murine model, probably related to the described cytotoxic activity, and, moreover, its presence in the essential oil from the leaves of C. regelianus could explain, at least in part, the ethnopharmacological use of this plant in the treatment of cancer. 相似文献
103.
Hélène C. Laude Barbara Jonchère Eve Maubec Agnès Carlotti Eduardo Marinho Benoit Couturaud Martine Peter Xavier Sastre-Garau Marie-Fran?oise Avril Nicolas Dupin Flore Rozenberg 《PLoS pathogens》2010,6(8)
Merkel Cell Polyomavirus (MCPyV) is associated with Merkel Cell carcinoma (MCC), a rare, aggressive skin cancer with neuroendocrine features. The causal role of MCPyV is highly suggested by monoclonal integration of its genome and expression of the viral large T (LT) antigen in MCC cells. We investigated and characterized MCPyV molecular features in MCC, respiratory, urine and blood samples from 33 patients by quantitative PCR, sequencing and detection of integrated viral DNA. We examined associations between either MCPyV viral load in primary MCC or MCPyV DNAemia and survival. Results were interpreted with respect to the viral molecular signature in each compartment. Patients with MCC containing more than 1 viral genome copy per cell had a longer period in complete remission than patients with less than 1 copy per cell (34 vs 10 months, P = 0.037). Peripheral blood mononuclear cells (PBMC) contained MCPyV more frequently in patients sampled with disease than in patients in complete remission (60% vs 11%, P = 0.00083). Moreover, the detection of MCPyV in at least one PBMC sample during follow-up was associated with a shorter overall survival (P = 0.003). Sequencing of viral DNA from MCC and non MCC samples characterized common single nucleotide polymorphisms defining 8 patient specific strains. However, specific molecular signatures truncating MCPyV LT were observed in 8/12 MCC cases but not in respiratory and urinary samples from 15 patients. New integration sites were identified in 4 MCC cases. Finally, mutated-integrated forms of MCPyV were detected in PBMC of two patients with disseminated MCC disease, indicating circulation of metastatic cells. We conclude that MCPyV molecular features in primary MCC tumour and PBMC may help to predict the course of the disease. 相似文献
104.
Tatiana Andreza da Silva Marinho Maria T. F. Piedade Florian Wittmann 《Wetlands Ecology and Management》2010,18(6):665-677
Amazonian white-water (várzea) floodplains harbor many commercially important timber species which in Brazil are harvested
following regulations of the Federal Environmental Agency (IBAMA). Although it is well-known that tree physiology, growth,
and species distribution of Amazonian floodplain trees is linked to the heights and durations of the periodical inundations,
information about timber stocks and population dynamics is lacking for most tree species. We investigated timber stocks and
the population structure of four intensely logged tree species in a western Brazilian várzea forest on an area totaling 7.5 ha.
Spatial distribution was investigated in all trees as a function of inundation height and duration and the distance to the
river channel, and additionally for saplings (trees <10 cm diameter at breast height––DBH) as a function of the relative photosynthetically
active radiation (rPAR). The diameter-class distribution in Hura crepitans and Ocotea cymbarum indicated that populations are subject to density variations that possibly are traced to small-scale flood variability. In
all species, saplings concentrated at higher topographic elevations than the mature tree populations, which suggest that the
physical ‘escape’ from a flooded environment is an important acclimation to flooding. While Ocotea cymbarum and Guarea guidonia were high-density wood species characterized by random dispersion and a pronounced shade-tolerance, Hura crepitans and Sterculia apetala presented lower wood density, aggregated dispersion, and were more light-demanding. All species presented exploitable stems
according to the current harvest regulations, with elevated abundances in comparison to other Amazonian forest types. However,
stem densities are below the harvest rates indicating that the harvest regulations are not sustainable. We recommend that
the forest management in várzea forests should include specific establishment rates of timber species in dependence of the
peculiar site conditions to achieve sustainability. 相似文献
105.
Caroline Da Ros Montes D’Oca Tatiane Coelho Tamara Germani Marinho Carolina Rosa Lopes Hack Rodrigo da Costa Duarte Pedro Almeida da Silva Marcelo Gonçalves Montes D’Oca 《Bioorganic & medicinal chemistry letters》2010,20(17):5255-5257
This work reports the synthesis of new fatty acid amides from C16:0, 18:0, 18:1, 18:1 (OH), and 18:2 fatty acids families with cyclic and acyclic amines and demonstrate for the first time the activity of these compounds as antituberculosis agents against Mycobacterium tuberculosis H37Rv, M. tuberculosis rifampicin resistance (ATCC 35338), and M. tuberculosis isoniazid resistance (ATCC 35822). The fatty acid amides derivate from ricinoleic acid were the most potent one among a series of tested compounds, with a MIC 6.25 μg/mL for resistance strains. 相似文献
106.
Leme Flávia Maria Borella Pedro Henrique Marinho Cristina Ribeiro Teixeira Simone Pádua 《Protoplasma》2020,257(4):1183-1199
Protoplasma - Cannabaceae is a known family because of the production of cannabinoids in laticifers and glandular trichomes of Cannabis sativa. Laticifers are latex-secreting structures, which in... 相似文献
107.
Thaís Augusto Marinho Carmen Luci Rodrigues Lopes Sheila Araújo Teles Nádia Rúbia Silva Reis Megmar Aparecida dos Santos Carneiro Andreia Alves de Andrade Regina Maria Bringel Martins 《Memórias do Instituto Oswaldo Cruz》2013,108(4):519-522
The prevalence of hepatitis C virus (HCV) in a population of
recyclable waste collectors (n = 431) was assessed using a cross-sectional
survey in all 15 cooperatives in the city of Goiânia, Central-West Brazil. The
HCV prevalence was 1.6% (95% confidence interval: 0.6-3.6) and a history of
sexually transmitted infections was independently associated with this
infection. HCV RNA (corresponding to genotype 1; subtypes 1a and 1b) was
detected in five/seven anti-HCV-positive samples. Although the study population
reported a high rate (47.3%) of sharps and needle accidents, HCV infection was
not more frequent in recyclable waste collectors than in the general Brazilian
population. 相似文献
108.
Pedro Marinho Carlos E. Costa-Campos Patrick R. Sanches Thiago R. de Carvalho 《Studies on Neotropical Fauna and Environment》2013,48(3):236-238
The bufonid genus Rhaebo contains 13 species, Rhaebo guttatus being one of the two species that occur in Brazil. Here we describe for the first time the release call of R. guttatus from the Brazilian Amazonia. Release calls are highly variable both in the temporal and in the frequency domains. The advertisement and release calls of Rhaebo guttatus are distinguished from each other by their temporal envelope, distribution of sound energy, and calling emission patterns. The acoustic characterization of release calls in Rhaebo could be potentially informative in the taxonomy of this genus, as previously reported for members of the bufonid genus Rhinella. 相似文献
109.
Maria Beatriz Barbosa de Barros-Barreto Luciana Cavalcante Marinho Renata Perpétuo Reis Camila Souza da Mata Paulo Cavalcante Gomes Ferreira 《Journal of applied phycology》2013,25(4):1143-1149
Kappaphycus alvarezii which is endemic to the Indo-Pacific region is the main raw material for kappa carrageenan production. A seedling that was cultivated in Japan (originally from the Philippines) was introduced in a trial, for aquaculture purpose, in 1995, in São Paulo State, southeastern region of Brazil. It was later introduced in Santa Catarina State, in Southern Brazil. In 1998, another seedling that was brought from Venezuela, also originally from the Philippines, was commercially introduced at Ilha Grande Bay and later on at Sepetiba Bay, both in Rio de Janeiro State. The aim of this study was to characterize 21 samples from different farms (Rio de Janeiro and Santa Catarina States) and verify if they are K. alvarezii or other species. Based on the intergenic spacer cox2-3 sequences, phylogenetic relationships were inferred through neighbor joining, maximum likelihood, and Bayesian analyses. The topology of the trees suggests that all samples from the different farms form a monophyletic group of K. alvarezii. Statistical analysis of the cox2-3 marker calculated with median-joining network showed 38 variable positions defining 15 haplotypes for the studied samples of Kappaphycus. The most frequent K. alvarezii haplotype grouped the samples cultivated worldwide with the Brazilian samples. These results are important for better productivity and are environmentally desirable for introduction purposes since the species introduced is known and will help focusing the research on this species. This knowledge can be of assistance to the government in setting up environmental and cultivation protocols to achieve sustainability in macroalgae aquaculture. 相似文献
110.
Camila Devicaro de Souza Cristina Ribeiro Marinho Simone Pádua Teixeira 《Trees - Structure and Function》2013,27(3):801-813
Robust glandular appendages are reported in legumes of the Caesalpinieae tribe. Most studies only attempt to describe the external morphology of these structures, without providing a distinction between glandular trichomes and emergences. This study employed ontogeny to resolve the terminology of these structures present in flowers of two tropical woody legumes of Caesalpinieae, Erythrostemon gilliesii and Poincianella pluviosa, through surface, anatomical and ultrastructural analyses. Flowers of both species exhibit branched and non-branched glandular trichomes since these structures originate from a single protodermal cell. Non-branched glandular trichomes occur on the inflorescence axis, pedicel, sepals and ovary; in P. pluviosa, they also occur in the unguicle of wings and standard, filaments, anthers and style. This type of trichome shows a non-secretory multiseriate stalk and a secretory multicellular head. Branched glandular trichomes, with similar morphology but exhibiting non-secretory branches, occur in the inflorescence axis, pedicel and sepals; in P. pluviosa, they also occur in the unguicle of wings. During the secretory phase, the trichome head cells have large nuclei, cytoplasm rich in vacuoles, oleoplasts, mitochondria, rough endoplasmic reticulum and free ribosomes. The content is released in the intercellular spaces of the head in a merocrinous mechanism and reaches the surface through cuticle rupture. We emphasized the importance of ontogenetic studies to clarify the terminology of secretory structures. This type of study should be performed in other caesalpinoids so that such robust glandular appendages can be correctly interpreted and used with phylogenetic value in the group. 相似文献