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11.
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Impacts of forestry planting on primary production in upland lakes from north‐west Ireland 下载免费PDF全文
Mark A. Stevenson Suzanne McGowan N. John Anderson Robert H. Foy Peter R. Leavitt Yvonne R. McElarney Daniel R. Engstrom Sergi Pla‐Rabés 《Global Change Biology》2016,22(4):1490-1504
Planted forests are increasing in many upland regions worldwide, but knowledge about their potential effects on algal communities of catchment lakes is relatively unknown. Here, the effects of afforestation were investigated using palaeolimnology at six upland lake sites in the north‐west of Ireland subject to different extents of forest plantation cover (4–64% of catchment area). 210Pb‐dated sediment cores were analysed for carotenoid pigments from algae, stable isotopes of bulk carbon (δ13C) and nitrogen (δ15N), and C/N ratios. In lakes with >50% of their catchment area covered by plantations, there were two‐ to sixfold increases in pigments from cryptophytes (alloxanthin) and significant but lower increases (39–116%) in those from colonial cyanobacteria (canthaxanthin), but no response from biomarkers of total algal abundance (β‐carotene). In contrast, lakes in catchments with <20% afforestation exhibited no consistent response to forestry practices, although all lakes exhibited fluctuations in pigments and geochemical variables due to peat cutting and upland grazing prior to forest plantation. Taken together, patterns suggest that increases in cyanobacteria and cryptophyte abundance reflect a combination of mineral and nutrient enrichment associated with forest fertilization and organic matter influx which may have facilitated growth of mixotrophic taxa. This study demonstrates that planted forests can alter the abundance and community structure of algae in upland humic lakes of Ireland and Northern Ireland, despite long histories of prior catchment disturbance. 相似文献
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The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close
association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441–447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection to other organelles
as appropriate. In the post-Golgi direction, these include vacuole and plasma membrane, and specialized transport routes to
each are required to prevent mislocalization. Recent evidence in plant cells points to the existence of post-Golgi organelles
that function as intermediate stations for efficient protein traffic, as well as to the influence of small GTPases such as
Rabs and ARFs on post-Golgi trafficking. This review focuses on the latest findings on post-Golgi trafficking routes and on
the involvement of GTPases and their effectors on the trafficking of proteins in the plant secretory pathway.
Sally L. Hanton and Loren A. Matheson have contributed equally to this work. 相似文献
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Thioredoxin/fructose-1,6-bisphosphatase affinity in the enzyme activation by the ferredoxin-thioredoxin system 总被引:2,自引:0,他引:2
In this work we analyze the affinity relationship between photosynthetic fructose-1,6-bisphosphatase and ferredoxin and thioredoxin from spinach leaves, two components of the proposed light-activation system of this enzyme, using affinity techniques on ferredoxin- and thioredoxin-Sepharose columns. Oxidized and reduced ferredoxin did not show enzyme affinity, whereas thioredoxin, both the oxidized and the dithiothreitol-reduced form, exhibited a strong bisphosphatase affinity at pH 7.5; this thioredoxin/enzyme affinity appears diminished at pH 8.2. When the affinity experiments were performed in the presence of 5 mM Mg2+, only 30% and 12% of the bisphosphatase remained bound to the thioredoxin-Sepharose at pH 7.5 and 8.0, respectively; these percentages were reduced to 6% when the Mg2+ concentration increased to 10 mM. These results suggest that a rise of stromal pH and Mg2+ concentration can account for a loosening of the thioredoxin/bisphosphatase linkage, which could be of physiological significance in the dark-light transition. Studies on the nature of the chemical groups responsible for the affinity have shown that the thioredoxin/bisphosphatase linkage is concerned with the existence of hydrophobic clusters. We have found no difference in the behaviour of the chloroplastic thioredoxins f and m, and the cytoplasmic ones cf and cm. These results support the existence of an in vivo thioredoxin/fructose-1,6-bisphosphatase interaction, in accordance with the light-activation mechanism by the ferredoxin-thioredoxin system. 相似文献
17.
Marika Crohns Seppo Saarelainen Jukka Laitinen Kimmo Peltonen Hannu Alho Pirkko Kellokumpu-Lehtinen 《Free radical research》2013,47(10):965-974
To examine lipid peroxidation during radiotherapy (RT), exhaled pentane samples were collected from 11 lung cancer patients before RT and 30 and 120 min after the start of RT on days 1, 4 and 5 and at 30 and 40 Grays, if possible. Exhaled pentane samples were collected once from 30 healthy controls. Serum thiobarbituric-acid-reactive substances (TBARS) and conjugated dienes (CD) were obtained from patients on each exhaled air collection day. Lung cancer patients had higher exhaled pentane levels than controls (1.73 ng/L vs 0.83 ng/L, p=0.017). Exhaled pentane levels tended to decrease during the first RT day (p=0.075) and levels of CD decreased during the first week of RT (p=0.014). Higher pre-treatment pentane levels predicted better survival (p=0.003). Elevated exhaled pentane levels before RT may be due to the lipid peroxidation burden associated with cancer. The decrease of lipid peroxidation markers during RT may be attributable to enhanced antioxidant defense mechanisms. 相似文献
18.
Maria Mercedes Nogueras Immaculada Pons Ana Ortu?o Jaime Miret Julia Pla Joaquim Castellà Ferran Segura 《PloS one》2013,8(8)
Background
Rickettsia typhi is the etiological agent of murine typhus (MT), a disease transmitted by two cycles: rat-flea-rat, and peridomestic cycle. Murine typhus is often misdiagnosed and underreported. A correct diagnosis is important because MT can cause severe illness and death. Our previous seroprevalence results pointed to presence of human R . typhi infection in our region; however, no clinical case has been reported. Although cats have been related to MT, no naturally infected cat has been described. The aim of the study is to confirm the existence of R . typhi in our location analyzing its presence in cats and fleas.Methodology/Principal Findings
221 cats and 80 fleas were collected from Veterinary clinics, shelters, and the street (2001-2009). Variables surveyed were: date of collection, age, sex, municipality, living place, outdoor activities, demographic area, healthy status, contact with animals, and ectoparasite infestation. IgG against R . typhi were evaluated by indirect immunofluorescence assay. Molecular detection in cats and fleas was performed by real-time PCR. Cultures were performed in those cats with positive molecular detection. Statistical analysis was carried out using SPSS. A p < 0.05 was considered significant.Thirty-five (15.8%) cats were seropositive. There were no significant associations among seropositivity and any variables. R . typhi was detected in 5 blood and 2 cultures. High titres and molecular detection were observed in stray cats and pets, as well as in spring and winter. All fleas were Ctenocephalides felis. R . typhi was detected in 44 fleas (55%), from shelters and pets. Co-infection with R . felis was observed.Conclusions
Although no clinical case has been described in this area, the presence of R . typhi in cats and fleas is demonstrated. Moreover, a considerable percentage of those animals lived in households. To our knowledge, this is the first time R . typhi is detected in naturally infected cats. 相似文献19.
20.
Jérémy Jabiol Andreas Bruder Mark O. Gessner Marika Makkonen Brendan G. McKie Edwin T.H.M. Peeters Veronique C.A. Vos Eric Chauvet 《Fungal Ecology》2013,6(5):439-448
Information about the global distribution of aquatic hyphomycetes is scarce, despite the primary importance of these fungi in stream ecosystem functioning. In particular, the relationship between their diversity and latitude remains unclear, due to a lack of coordinated surveys across broad latitudinal ranges. This study is a first report on latitudinal patterns of aquatic hyphomycete diversity associated with native leaf-litter species in five streams located along a gradient extending from the subarctic to the tropics. Exposure of leaf litter in mesh bags of three different mesh sizes facilitated assessing the effects of including or excluding different size-classes of litter-consuming invertebrates. Aquatic hyphomycete evenness was notably constant across all sites, whereas species richness and diversity, expressed as the Hill number, reached a maximum at mid-latitudes (Mediterranean and temperate streams). These latitudinal patterns were consistent across litter species, despite a notable influence of litter identity on fungal communities at the local scale. As a result, the bell-shaped distribution of species richness and Hill diversity deviated markedly from the latitudinal patterns of most other groups of organisms. Differences in the body-size distribution of invertebrate communities colonizing the leaves had no effect on aquatic hyphomycete species richness, Hill diversity or evenness, but invertebrates could still influence fungal communities by depleting litter, an effect that was not captured by the design of our experiment. 相似文献