全文获取类型
收费全文 | 342篇 |
免费 | 17篇 |
出版年
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 5篇 |
2020年 | 3篇 |
2019年 | 9篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 7篇 |
2015年 | 16篇 |
2014年 | 17篇 |
2013年 | 17篇 |
2012年 | 29篇 |
2011年 | 28篇 |
2010年 | 22篇 |
2009年 | 27篇 |
2008年 | 22篇 |
2007年 | 24篇 |
2006年 | 19篇 |
2005年 | 21篇 |
2004年 | 15篇 |
2003年 | 17篇 |
2002年 | 16篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1981年 | 1篇 |
1978年 | 2篇 |
排序方式: 共有359条查询结果,搜索用时 59 毫秒
351.
Pierrick Thoraval Marielle Afanassieff Francois-Loic Cosset Frederic Lasserre Gerard Verdier Francoise Coudert Ginette Dambrine 《Transgenic research》1995,4(6):369-377
We have used vectors derived from avian leukosis viruses to transduce exogenous genes into early somatic stem cells of chicken embryos. The ecotropic helper cell line, Isolde, was used to generate stocks of NL-B vector carrying theNeo
r
selectable marker and theEscherichia coli lacZ gene. Microinjection of the NL-B vector directly beneath unincubated chicken embryo blastoderms resulted in infection of germline stem cells. One of the 16 male birds hatched (6.25%) from the injected embryos contained vector DNA sequences in its semen. Vector sequences were transmitted to G1 progeny at a frequency of 2.7%.Neo
r andlacZ genes were transcribedin vitro in chicken embryo fibroblast cultures from transgenic embryos of the G2 progeny. 相似文献
352.
Marielle Sanmarco 《Bio Tribune Magazine》2003,7(1):24-25
Le syndrome des anti-phospholipides (SAPL) est la cause la plus fréquente de thrombophilie. C’est une maladie auto-immune
définie depuis 1987 par l’association d’anomalies cliniques thrombotiques ou obstétricales avec la présence persistante d’anticorps
anti-phospholipides (aPLs). La récurrence des évènements cliniques constitue le caractère aggravant du SAPL qui atteint le
plus souvent le sujet jeune (<45 ans) et préférentiellement la femme. Ce syndrome peut être primaire (SAPL I) ou secondaire
(SAPL II) à une maladie auto-immune, principalement le lupus. 相似文献
353.
Takahashi Juliana Possatto Fernandes Feliciano Leticia Marielle Santos Dayane Cristina Silva Ramos Silézia Oliveira Rogério A. Attili-Angelis Derlene Rodrigues Nadia Regina Sampaio Jorge Luiz Mello dos Anjos Martins Marilena Melhem Marcia Souza Carvalho 《Current fungal infection reports》2020,14(1):9-14
Current Fungal Infection Reports - Acquired resistance among fungal strains is a growing concern. The reasons for the emergence of this phenomenon, with great clinical implications, are only... 相似文献
354.
355.
Marielle K. Bouyou-Akotet 《Experimental parasitology》2009,123(3):265-268
The ability of natural killer (NK) cells to produce gamma interferon (IFN-γ) after ex vivo stimulation with crude schizont lysate of Plasmodium falciparum was studied in uninfected and P. falciparum-infected pregnant Gabonese women segregated according to the gravidity at the time of delivery. This activity was measured in purified NK cells as well as in whole blood from the periphery and cord. Crude schizont lysate-stimulated NK cells from primiparous women produced significantly more IFN-γ than those from multiparous women (P < 0.001). Women with malaria infection produced more IFN-γ than negative women in peripheral blood (P < 0.001) indicating that immunological determinants regulating the susceptibility to malaria in pregnant women are parasite-specific. These findings reveal that NK cells are major source of IFN-γ when exposed to P. falciparum antigens in vitro in absence of any other co-stimulant. 相似文献
356.
T Reid Alderson Elias Adriaenssens Bob Asselbergh Iva Pritianac Jonas Van Lent Heidi Y Gastall Marielle A Wlti John M Louis Vincent Timmerman Andrew J Baldwin Justin LP Benesch 《The EMBO journal》2021,40(8)
HSP27 is a human molecular chaperone that forms large, dynamic oligomers and functions in many aspects of cellular homeostasis. Mutations in HSP27 cause Charcot‐Marie‐Tooth (CMT) disease, the most common inherited disorder of the peripheral nervous system. A particularly severe form of CMT disease is triggered by the P182L mutation in the highly conserved IxI/V motif of the disordered C‐terminal region, which interacts weakly with the structured core domain of HSP27. Here, we observed that the P182L mutation disrupts the chaperone activity and significantly increases the size of HSP27 oligomers formed in vivo, including in motor neurons differentiated from CMT patient‐derived stem cells. Using NMR spectroscopy, we determined that the P182L mutation decreases the affinity of the HSP27 IxI/V motif for its own core domain, leaving this binding site more accessible for other IxI/V‐containing proteins. We identified multiple IxI/V‐bearing proteins that bind with higher affinity to the P182L variant due to the increased availability of the IxI/V‐binding site. Our results provide a mechanistic basis for the impact of the P182L mutation on HSP27 and suggest that the IxI/V motif plays an important, regulatory role in modulating protein–protein interactions. 相似文献
357.
Anelia Iantcheva Mireille Chabaud Viviane Cosson Marielle Barascud Bernadette Schutz Catherine Primard-Brisset Patricia Durand David G. Barker Mariana Vlahova Pascal Ratet 《Plant cell reports》2009,28(10):1563-1572
Insertion mutant collections are powerful tools for genetic studies in plants. Although large-scale insertional mutagenesis
using T-DNA is not feasible in legumes, the Tnt1 tobacco retrotransposon can be used as a very efficient mutagen in the Medicago truncatula R108 genotype. In this article, we show that Tnt1 can also be exploited to create insertional mutants via transformation and/or regeneration in the reference cultivar Jemalong.
Tnt1 insertional mutagenesis in Jemalong following Agrobacterium tumefaciens-mediated transformation was found to be very efficient, with an average of greater than 15 insertions/line. In contrast,
regeneration using low-copy transgenic starter lines resulted in a highly variable rate of new Tnt1 insertions. With the goal of increasing the number of additional Tnt1 insertions during regeneration of starter lines, we have compared the insertion frequencies for a number of different regeneration
protocols. In addition, we have been able to show that sucrose-mediated osmotic shock preceding regeneration significantly
increases the transposition frequency. Under optimal conditions, 95% of the regenerated Jemalong plants possess new insertions. 相似文献
358.
Marielle C Gold Heather D Ehlinger Matthew S Cook Susan K Smyk-Pearson Paul T Wille Ross M Ungerleider Deborah A Lewinsohn David M Lewinsohn 《PLoS pathogens》2008,4(2)
The control of Mycobacterium tuberculosis (Mtb) infection is heavily dependent on the adaptive Th1 cellular immune response. Paradoxically, optimal priming of the Th1 response requires activation of priming dendritic cells with Th1 cytokine IFN-γ. At present, the innate cellular mechanisms required for the generation of an optimal Th1 T cell response remain poorly characterized. We hypothesized that innate Mtb-reactive T cells provide an early source of IFN-γ to fully activate Mtb-exposed dendritic cells. Here, we report the identification of a novel population of Mtb-reactive CD4− αβTCR+ innate thymocytes. These cells are present at high frequencies, respond to Mtb-infected cells by producing IFN-γ directly ex vivo, and display characteristics of effector memory T cells. This novel innate population of Mtb-reactive T cells will drive further investigation into the role of these cells in the containment of Mtb following infectious exposure. Furthermore, this is the first demonstration of a human innate pathogen-specific αβTCR+ T cell and is likely to inspire further investigation into innate T cells recognizing other important human pathogens. 相似文献
359.