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151.
Thierry Cachot Martina Müller Marie-Noëlle Pons 《Applied microbiology and biotechnology》1991,35(4):450-454
Summary The kinetics of ethanol, acetaldehyde, ethyl acetate and fusel alcohols during alcoholic fermentations on cane molasses by Saccharomyces cerevisiae have been obtained via an in-situ gas membrane sensor connected to a gas chromatograph. Various operation parameters have been investigated such as inoculum rate, molasses concentration, operation mode (batch, fed-batch). The modification of fusel alcohols kinetics in response to addition of amino acids has been studied as well as the assimilation of two intermediary aldehydes (isovaleraldehyde and isobutyraldehyde) in the fusel alcohol synthesis pathway.
Offprint requests to: M.-N. Pons 相似文献
152.
Anne Groboillot Marie-Noëlle Pons Jean-Marc Engasser 《Applied microbiology and biotechnology》1989,32(1):37-44
Summary Gas membrane sensors, connected to a gas chromatography, have been tested in fermentation on complex media of industrial interest. Selectivity, sensitivity and reliability have been assessed: the calibration on fermentation medium is recommended. The use of such a sensor is demonstrated in the study of the kinetics of batch and fed-batch alcoholic fermentations on beet molasses. The kinetics of acetaldehyde are especially pointed out in relation to ethanol, fusel alcohols and carbon dioxide production.Offprint requests to: M.-N. Pons 相似文献
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Hayat Hage Shingo Miyauchi Máté Virágh Elodie Drula Byoungnam Min Delphine Chaduli David Navarro Anne Favel Manon Norest Laurence Lesage-Meessen Balázs Bálint Zsolt Merényi Laura de Eugenio Emmanuelle Morin Angel T. Martínez Petr Baldrian Martina Štursová María Jesús Martínez Cenek Novotny Jon K. Magnuson Joey W. Spatafora Sundy Maurice Jasmyn Pangilinan Willian Andreopoulos Kurt LaButti Hope Hundley Hyunsoo Na Alan Kuo Kerrie Barry Anna Lipzen Bernard Henrissat Robert Riley Steven Ahrendt László G. Nagy Igor V. Grigoriev Francis Martin Marie-Noëlle Rosso 《Environmental microbiology》2021,23(10):5716-5732
156.
Telomerase-negative yeasts survive via one of the two Rad52-dependent recombination pathways, which have distinct genetic requirements. Although the telomere pattern of type I and type II survivors is well characterized, the mechanistic details of short telomere rearrangement into highly evolved pattern observed in survivors are still missing. Here, we analyze immediate events taking place at the abruptly shortened VII-L and native telomeres. We show that short telomeres engage in pairing with internal Rap1-bound TG1–3-like tracts present between subtelomeric X and Y′ elements, which is followed by BIR-mediated non-reciprocal translocation of Y′ element and terminal TG1–3 repeats from the donor end onto the shortened telomere. We found that choice of the Y′ donor was not random, since both engineered telomere VII-L and native VI-R acquired Y′ elements from partially overlapping sets of specific chromosome ends. Although short telomere repair was associated with transient delay in cell divisions, Y′ translocation on native telomeres did not require Mec1-dependent checkpoint. Furthermore, the homeologous pairing between the terminal TG1–3 repeats at VII-L and internal repeats on other chromosome ends was largely independent of Rad51, but instead it was facilitated by Rad59 that stimulates Rad52 strand annealing activity. Therefore, Y′ translocation events taking place during presenescence are genetically separable from Rad51-dependent Y′ amplification process that occurs later during type I survivor formation. We show that Rad59-facilitated Y′ translocations on X-only telomeres delay the onset of senescence while preparing ground for type I survivor formation. 相似文献