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301.
Exogenous abscisic acid fate during maturation of hybrid larch (Larix×leptoeuropaea) somatic embryos
The maturation of somatic embryogenesis of hybrid larch is an essential step for plantlet production. ABA controls not only synchronicity of maturation, but has important consequences on eventual viability of the plantlet. To gain understanding of the role of this plant growth regulator during the maturation process of hybrid larch somatic embryos, we studied the incorporation of [ 3 H]-(±)-abscisic acid [tritiated (±)-ABA] over 6 weeks of culture. Results showed a rapid incorporation of label into the tissues directly in contact with the culture medium. Accumulation of tritiated (±)-ABA occurred mainly in the maturing somatic embryos found at the periphery of the embryogenic mass but not in direct contact with the culture medium. Tritiated (±)-ABA was mainly conjugated as a glucose ester form. Rates of tritium incorporation indicated a significant build-up in tritiated (±)-ABA metabolization at the third week of culture. The weekly measurement of labelling in the culture medium over 6 weeks showed no localized exhaustion of tritiated (±)-ABA in positions where the embryogenic masses were placed in Petri dishes. The calculated ABA internal content of the maturing somatic embryos was similar to published ELISA measurements of ABA. This result suggests an absence of endogenous ABA synthesis by somatic embryos of hybrid larch during maturation. 相似文献
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A simple procedure for the preparation of soluble human succinate dehydrogenase is described. These preparations have proved suitable for analysis by zone electrophoresis, using a specific stain to detect activity after separation. In a survey of succinate dehydrogenase from various tissues and different individuals, no evidence for genetic heterogeneity due to the expression of either multiple loci or alternative alleles at the succinate dehydrogenase locus was found. However, epigenetic heterogeneity in both molecular size and charge was seen and various explanations for the occurrence of the isoenzymes are explored. Estimates of molecular size (93,300 ± 9100) suggest that the smallest active unit of succinate dehydrogenase accounts for the major part of the solubilized activity. Kinetic studies have shown that the apparent K
m
values for succinate (0.9mm) and PMS (0.4mm) are comparable to those previously described for the beef heart enzyme, and these parameters were not significantly altered when the enzyme was removed from the membrane milieu. However a marked non-succinate-dependent activation of the membrane-associated enzyme at 38C is apparently lost on solubilization, and this observation may have some bearing on earlier reports of an apparent decrease in V
max on solubilization of succinate dehydrogenase. 相似文献
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Isma?l Ahmed Pei-Chen Lee Christina M. Lill Susan Searles Nielsen Fanny Artaud Lisa G. Gallagher Marie-Anne Loriot Claire Mulot Magali Nacfer Tian Liu Joanna M. Biernacka Sebastian Armasu Kari Anderson Federico M. Farin Christina Funch Lassen Johnni Hansen J?rgen H. Olsen Lars Bertram Demetrius M. Maraganore Harvey Checkoway Beate Ritz Alexis Elbaz 《PLoS genetics》2014,10(11)
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Fan Zhang Jessica L. Weckhorst Adrien Assié Ciara Hosea Christopher A. Ayoub Anastasia S. Khodakova Mario Loeza Cabrera Daniela Vidal Vilchis Marie-Anne Félix Buck S. Samuel 《Current biology : CB》2021,31(12):2603-2618.e9
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