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排序方式: 共有281条查询结果,搜索用时 15 毫秒
101.
Leverentz B Conway WS Janisiewicz W Abadias M Kurtzman CP Camp MJ 《Applied and environmental microbiology》2006,72(2):1135-1140
Fresh-cut apples contaminated with either Listeria monocytogenes or Salmonella enterica serovar Poona, using strains implicated in outbreaks, were treated with one of 17 antagonists originally selected for their ability to inhibit fungal postharvest decay on fruit. While most of the antagonists increased the growth of the food-borne pathogens, four of them, including Gluconobacter asaii (T1-D1), a Candida sp. (T4-E4), Discosphaerina fagi (ST1-C9), and Metschnikowia pulcherrima (T1-E2), proved effective in preventing the growth or survival of food-borne human pathogens on fresh-cut apple tissue. The contaminated apple tissue plugs were stored for up to 7 days at two different temperatures. The four antagonists survived or grew on the apple tissue at 10 or 25 degrees C. These four antagonists reduced the Listeria monocytogenes populations and except for the Candida sp. (T4-E4), also reduced the S. enterica serovar Poona populations. The reduction was higher at 25 degrees C than at 10 degrees C, and the growth of the antagonists, as well as pathogens, increased at the higher temperature. 相似文献
102.
Juliano M. Vieira Fabiano B. Carvalho Jessié M. Gutierres Mayara S. P. Soares Pathise S. Oliveira Maribel A. Rubin 《Redox report : communications in free radical research》2017,22(6):493-500
Objective: Here we investigated the impact of chronic high-intensity interval training (HIIT) and caffeine consumption on the activities of Na+-K+-ATPase and enzymes of the antioxidant system, as well as anxiolytic-like behaviour in the rat brain.Methods: Animals were divided into groups: control, caffeine (4?mg/kg), caffeine (8?mg/kg), HIIT, HIIT plus caffeine (4?mg/kg) and HIIT plus caffeine (8?mg/kg). Rats were trained three times per week for 6 weeks, and caffeine was administered 30 minutes before training. We assessed the anxiolytic-like behaviour, Na+-K+-ATPase, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, levels of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) in the brain.Results and discussion: HIIT-induced anxiolytic-like behaviour increased Na+-K+-ATPase and GPx activities and TBARS levels, altered the activities of SOD and CAT in different brain regions, and decreased GSH levels. Caffeine, however, elicited anxiogenic-like behaviour and blocked HIIT effects. The combination of caffeine and HIIT prevented the increase in SOD activity in the cerebral cortex and GPx activity in three brain regions. Our results show that caffeine promoted anxiogenic behaviour and prevented HIIT-induced changes in the antioxidant system and Na+-K+-ATPase activities. 相似文献
103.
Identification of Mutations Contributing to the Temperature-Sensitive, Cold-Adapted, and Attenuation Phenotypes of the Live-Attenuated Cold-Passage 45 (cp45) Human Parainfluenza Virus 3 Candidate Vaccine 总被引:2,自引:0,他引:2 下载免费PDF全文
104.
Maribel L. Dionisio-Sese Atsuhiro Shimada Tadashi Maruyama Shigetoh Miyachi 《Archives of microbiology》1993,159(1):1-5
The prokaryotic algal symbiont of ascidians, Prochloron sp., was found to exhibit carbonic anhydrase activity which is largely associated with the cell surface. This extracellular carbonic anhydrase activity was inhibited, while the intracellular activity was not affected, by chloride or bromide. Acetazolamide and ethoxyzolamide inhibited carbonic anhydrase activity with I50 values of 7×10-4 and 3×10-4M, respectively. These I50 values are similar to those observed for intracellular carbonic anhydrases of Synechococcus sp. PCC7942, Chlamydomonas reinhardii and spinach.Abbreviations AZA
acetazolamide
- CA
carbonic anhydrase
- chl
chlorophyll
- EZA
ethozyzolamide
- I50
concentration of an inhibitor required to cause 50% inhibition
- Rubisco
ribulose-1,5-bisphosphate carboxylase/oxygenase
- U
unit 相似文献
105.
Elena Chaboteaux Alejandro Lopera Toro Claudia Maribel Vega Adrian Forsyth 《Biotropica》2023,55(6):1081-1086
This study represents the first evidence of mercury contamination in the family Scarabaeidae, with a close focus on Coprophanaeus lancifer, the largest copro-necrophagous beetle in South America. This work shows the repartition of total mercury (THg) in the insect body and lays the groundwork for additional future studies. Abstract in Spanish is available with online material. 相似文献
106.
Variability of Leishmania (Leishmania) infantum among stocks from immunocompromised, immunocompetent patients and dogs in Spain 总被引:2,自引:0,他引:2
Maribel Jiménez Montserrat Ferrer-Dufol Carmen Cañavate Beatriz Gutiérrez-Solar Ricardo Molina Fernando Lagun Rogelio López-Vélez Emilia Cercenado Esteban Daudén Julio Blazquez Conception Ladrón de Guevara Javier Gómez Julian de la Torre Carlos Barros Jordi Altes Teresa Serra Jorge Alvar 《FEMS microbiology letters》1995,131(2):197-204
Abstract Leishmania (Leishmania) infantum is the causative agent of both the cutaneous and visceral forms of leishmaniasis in southwest Europe; the dog is the main reservoir. In order to identify the L. (L.) infantum zymodemes present in Spain, a total number of 85 Leishmania stocks isolated from dogs (31), HIV-positive patients (46) with visceral or cutaneous leishmaniasis, a patient with visceral leishmaniasis complicating renal transplantation (1) and immunocompetent patients (7) with visceral or cutaneous leishmaniasis, have been characterized by isoenzyme typing. All canine stocks were MON-1, which is the most widespread zymodeme in the Mediterranean area. In immunocompetent patients three zymodemes were found: MON-1 (2), MON-24 (2) and MON-34 (3). Nine different zymodemes were obtained in stocks from HTV co-infected patients, indicating a higher variability of L. (L.) infantum amongst them: MON-1 (in 21 stocks), MON-24 (7), MON-28 (1), MON-29 (3), MON-33 (7), MON-34 (1) and MON-183 (4). Two new zymodemes, MON-198 (1) and MON-199 (1), were described among HIV patients from Spain. The stock from the renal transplanted patient was MON-1. The exclusive presence of certain zymodemes in immunocompromised patients and their absence in typical cases of cutaneous and visceral 相似文献
107.
BMRP is a Bcl-2 binding protein that induces apoptosis 总被引:4,自引:0,他引:4
Chintharlapalli SR Jasti M Malladi S Parsa KV Ballestero RP González-García M 《Journal of cellular biochemistry》2005,94(3):611-626
Members of the Bcl-2 family of proteins play important roles in the regulation of cell death by apoptosis. The yeast Two-Hybrid system was utilized to identify a protein that interacts with the anti-apoptotic protein Bcl-2, designated BMRP. This protein corresponds to a previously known mitochondrial ribosomal protein (MRPL41). Binding experiments confirmed the interaction of BMRP to Bcl-2 in mammalian cells. Subcellular fractionation by differential centrifugation studies showed that both Bcl-2 and BMRP are localized to the same fractions (fractions that are rich in mitochondria). Northern blot analysis revealed a major bmrp mRNA band of approximately 0.8 kb in several human tissues. Additionally, a larger 2.2 kb mRNA species was also observed in some tissues. Western blot analysis showed that endogenous BMRP runs as a band of 16-17 kDa in SDS-PAGE. Overexpression of BMRP induced cell death in primary embryonic fibroblasts and NIH/3T3 cells. Transfection of BMRP showed similar effects to those observed by overexpression of the pro-apoptotic proteins Bax or Bad. BMRP-stimulated cell death was counteracted by co-expression of Bcl-2. The baculoviral caspase inhibitor p35 also protected cells from BMRP-induced cell death. These findings suggest that BMRP is a mitochondrial ribosomal protein involved in the regulation of cell death by apoptosis, probably affecting pathways mediated by Bcl-2 and caspases. 相似文献
108.
Pedro?A.?García-Saucedo Maribel?Valdez-Morales María?Elena Valverde Andrés?Cruz-Hernández Octavio?Paredes-LópezEmail author 《Plant Cell, Tissue and Organ Culture》2005,80(2):215-219
In vitro micropropagation Opuntia cactus plants, whose young stems are consumed as vegetables, is reported. Cladode explants from three Opuntia genotypes, were cultivated in MS medium containing BA and GA3. Shoots produced were used as secondary explants and BA added at different concentrations to induce shoot development. 0.5 M BA was the best for bud formation. Satisfactory rooting occurred when IBA was added to the medium, and plants were successfully established in soil and adapted to greenhouse conditions. The protocols developed in this work provide a basis for the establishment of a genetic transformation system for Opuntia spp. 相似文献
109.
Zamilpa A Herrera-Ruiz M del Olmo E López-Pérez JL Tortoriello J San Feliciano A 《Bioorganic & medicinal chemistry letters》2005,15(15):3483-3486
Anxiolytic effects induced by benzalphthalides on mice have been evaluated. The evaluation was based on the spent time and the number of entries of animals into the open arms in the elevated plus maze test. Single administration of benzalphthalides 1 and 11 induced significant increments in both parameters, thus revealing their potentiality as new leads for the development of non-benzodiazepinic and non-nitrogenated antianxiety agents. 相似文献
110.
A novel ARH splice site mutation in a Mexican kindred with autosomal recessive hypercholesterolemia 总被引:2,自引:0,他引:2
Canizales-Quinteros S Aguilar-Salinas CA Huertas-Vázquez A Ordóñez-Sánchez ML Rodríguez-Torres M Venturas-Gallegos JL Riba L Ramírez-Jimenez S Salas-Montiel R Medina-Palacios G Robles-Osorio L Miliar-García A Rosales-León L Ruiz-Ordaz BH Zentella-Dehesa A Ferré-D'Amare A Gómez-Pérez FJ Tusié-Luna MT 《Human genetics》2005,116(1-2):114-120