全文获取类型
收费全文 | 1345篇 |
免费 | 126篇 |
出版年
2023年 | 6篇 |
2022年 | 13篇 |
2021年 | 35篇 |
2020年 | 29篇 |
2019年 | 29篇 |
2018年 | 54篇 |
2017年 | 34篇 |
2016年 | 37篇 |
2015年 | 84篇 |
2014年 | 68篇 |
2013年 | 91篇 |
2012年 | 117篇 |
2011年 | 89篇 |
2010年 | 61篇 |
2009年 | 62篇 |
2008年 | 95篇 |
2007年 | 74篇 |
2006年 | 79篇 |
2005年 | 56篇 |
2004年 | 43篇 |
2003年 | 47篇 |
2002年 | 53篇 |
2001年 | 15篇 |
2000年 | 13篇 |
1999年 | 16篇 |
1998年 | 13篇 |
1997年 | 12篇 |
1996年 | 12篇 |
1995年 | 8篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1992年 | 11篇 |
1991年 | 7篇 |
1990年 | 14篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1974年 | 2篇 |
1968年 | 2篇 |
1964年 | 3篇 |
排序方式: 共有1471条查询结果,搜索用时 468 毫秒
211.
Massimiliano Porcelli Mariano Casu Adolfo Lai Giuseppe Saba Massimo Pinori Silvana Cappelletti Paolo Mascagni 《Biopolymers》1999,50(2):211-219
Under the hypotheses of a structurally related binding site for antagonists of G‐protein coupled receptors and the ability of cyclic pentapeptides of chiral sequence D 1L 2D 3D 4L 5 to form rigid structures with which probe the pharmacophoric specificity of these receptors, inhibitors of substance P were designed based on available structure–activity relationships. ITF 1565, cyclo[D ‐Trp1‐Pro2‐D ‐Lys3‐D ‐Trp4‐Phe5], antagonized substance P activity mediated by type 1 neurokinin receptor (NK1) whereas it acted weakly against NK2 and did not inhibit endothelin at all. The preferential conformation of the peptide was obtained from nmr spectroscopy and computer calculations, and shown to contain the same βII‐turn and γ′‐turn found in other cyclic pentapeptides with the same chiral sequence. The structure of the peptide was compared with that of the β‐D ‐glucose molecule that has been proposed as a semirigid scaffold for antagonists of G‐protein coupled receptors. The γ′‐turn of the cyclic peptide superimposed well with β‐D ‐glucose in the chair conformation. Furthermore, when the side chains were considered, the aromatic groups of the two molecules were found to generally overlap. These results support the view of G‐protein coupled receptors as possessing structurally similar binding sites for antagonists and suggest that cyclic pentapeptides of chiral sequence D 1L 2D 3D 4L 5 may be useful as semirigid scaffolds for the design of antagonists of this family of receptors. © 1999 John Wiley & Sons, Inc. Biopoly 50: 211–219, 1999 相似文献
212.
Juan Rull Solana Abraham Pablo Schlisermann Mariano Ordano Sergio Ovruski 《Journal of Insect Behavior》2017,30(4):439-453
The natural history and mating behavior of a species of tephritid fruit fly in the poorly studied genus Haywardina are described for the first time. Haywardina cuculi Hendel larvae were recovered over four field seasons from infested fruit of Vassobia breviflora (Sendtn.) Hunz, which constitutes a new host plant record for this species. Recovered pupae emerged as adults over 183 days on average, suggesting that most individuals became dormant. Adult flies engaged in sexual activity as soon as two days after emergence, were highly promiscuous, and displayed large variability in copulation duration. As for most tephritid species in the subtribe Carpomyini, H. cuculi exhibited a resource defense mating system. Fly activity peaked around noon. Copulation could last from 9 min to 17 h, with most copulations beginning in the afternoon and lasting until the following day. We discuss the potential significance of copulation duration variability in light of mate guarding and sperm competition hypothesis and outline future research to understand the evolution of life history and these behavioral strategies. 相似文献
213.
Mariano?Ucchesu Marco?SariguEmail authorView authors OrcID profile Carla?Del?Vais Ignazio?Sanna Guy?d’Hallewin Oscar?Grillo Gianluigi?Bacchetta 《Vegetation History and Archaeobotany》2017,26(5):539-549
During the archaeological excavations in the Phoenician and Punic settlement of Santa Giusta (Oristano, Sardinia, Italy), dating back to the 6th–2nd centuries bc, several Prunus fruitstones (endocarps) inside amphorae were recovered. The exceptional state of preservation of the waterlogged remains allowed morphometric measurements to be done by image analysis and statistical comparisons made with modern cultivated and wild Prunus samples collected in Sardinia. Digital images of modern and archaeological Prunus fruitstones were acquired with a flatbed scanner and analysed by applying image analysis techniques to measure 26 morphometric features. By applying stepwise linear discriminant analysis, a morphometric comparison was made between the archaeological fruitstones of Prunus and the modern ones collected in Sardinia. These analyses allowed identification of 53 archaeological fruitstones as P. spinosa and 11 as P. domestica. Moreover, the archaeological samples of P. spinosa showed morphometric similarities in 92.5% of the cases with the modern P. spinosa samples currently growing near the Phoenician and Punic site. Likewise, the archaeological fruitstones identified as P. domestica showed similarities with the modern variety of P. domestica called Sanguigna di Bosa which is currently cultivated near the village of Bosa. Currently, these findings represent the first evidence of P. domestica in Italy during the Phoenician and Punic periods. 相似文献
214.
215.
216.
217.
Hominoid chromosomal rearrangements on 17q map to complex regions of segmental duplication 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Genome biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Cardone MF Jiang Z D'Addabbo P Archidiacono N Rocchi M Eichler EE Ventura M 《Genome biology》2008,9(2):R28
Background
Chromosomal rearrangements, such as translocations and inversions, are recurrent phenomena during evolution, and both of them are involved in reproductive isolation and speciation. To better understand the molecular basis of chromosome rearrangements and their part in karyotype evolution, we have investigated the history of human chromosome 17 by comparative fluorescence in situ hybridization (FISH) and sequence analysis. 相似文献218.
Fölling J Bossi M Bock H Medda R Wurm CA Hein B Jakobs S Eggeling C Hell SW 《Nature methods》2008,5(11):943-945
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach. 相似文献
219.
220.
Santos R Mariano AC Rosas-Oliveira R Pascarelli B Machado EA Meyer-Fernandes JR Gondim KC 《Archives of insect biochemistry and physiology》2008,67(2):55-62
The processes of accumulation and mobilization of carbohydrate stores in eggs of Rhodnius prolixus were analyzed. During oogenesis, the total amounts of glycogen, glucose, and trehalose increased with an accumulation of proteins, especially when oocytes grew from 1.0 to 1.5 mm in length. At 2.0 mm length, when oocytes were ready for oviposition, nutrient reserves did not increase appreciably and trehalose content decreased. Mating did not affect the final content of carbohydrates or proteins in oocytes of mated and virgin females. A trehalase activity was detected in follicles containing vitellogenic oocytes, 1.0 and 1.5 mm length, in both mated and virgin females. This activity was extremely low in chorionated, 2.0-mm oocytes. After oviposition, glycogen content decreased in fertilized eggs, but not in unfertilized ones, and some was present in newly hatched nymphs. Glucose content remained constant in unfertilized eggs, but increased in fertilized ones, while total protein amount was constant in both groups after egg laying. 相似文献