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Willemijntje?A?HoogerwerfEmail author Helen?Lee?Hellmich Maria?Adelaide?Micci John?H?Winston Lei?Zou Pankaj?J?Pasricha 《BMC molecular biology》2002,3(1):2
Background
The proteinase-activated receptor 4 (PAR4) is a G-protein-coupled receptor activated by proteases such as thrombin and trypsin. Although activation of PAR4 has been shown to modulate rat gastrointestinal motility, the rat PAR4 sequence was unknown until now. This study aimed to identify the rat PAR4 cDNA. 相似文献22.
James F. White Kathryn I. Kingsley Kurt P. Kowalski Ivelisse Irizarry April Micci Marcos A. Soares Marshall S. Bergen 《Plant and Soil》2018,422(1-2):195-208
Background and aims
Non-native Phragmites australis (haplotype M) is an invasive grass that decreases biodiversity and produces dense stands. We hypothesized that seeds of Phragmites carry microbes that improve seedling growth, defend against pathogens and maximize capacity of seedlings to compete with other plants.Methods
We isolated bacteria from seeds of Phragmites, then evaluated representatives for their capacities to become intracellular in root cells, and their effects on: 1.) germination rates and seedling growth, 2.) susceptibility to damping-off disease, and 3.) mortality and growth of competitor plant seedlings (dandelion (Taraxacum officionale F. H. Wigg) and curly dock (Rumex crispus L.)).Results
Ten strains (of 23 total) were identified and characterized; seven were identified as Pseudomonas spp. Strains Sandy LB4 (Pseudomonas fluorescens) and West 9 (Pseudomonas sp.) entered root meristems and became intracellular. These bacteria improved seed germination in Phragmites and increased seedling root branching in Poa annua. They increased plant growth and protected plants from damping off disease. Sandy LB4 increased mortality and reduced growth rates in seedlings of dandelion and curly dock.Conclusions
Phragmites plants associate with endophytes to increase growth and disease resistance, and release bacteria into the soil to create an environment that is favorable to their seedlings and less favorable to competitor plants.23.
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Deborah R. Boone Maria-Adelaide Micci Isabella G. Taglialatela Judy L. Hellmich Harris A. Weisz Min Bi Donald S. Prough Douglas S. DeWitt Helen L. Hellmich 《PloS one》2015,10(5)
Cognitive deficits in survivors of traumatic brain injury (TBI) are associated with irreversible neurodegeneration in brain regions such as the hippocampus. Comparative gene expression analysis of dying and surviving neurons could provide insight into potential therapeutic targets. We used two pathway-specific PCR arrays (RT2 Profiler Apoptosis and Neurotrophins & Receptors PCR arrays) to identify and validate TBI-induced gene expression in dying (Fluoro-Jade-positive) or surviving (Fluoro-Jade- negative) pyramidal neurons obtained by laser capture microdissection (LCM). In the Apoptosis PCR array, dying neurons showed significant increases in expression of genes associated with cell death, inflammation, and endoplasmic reticulum (ER) stress compared with adjacent, surviving neurons. Pro-survival genes with pleiotropic functions were also significantly increased in dying neurons compared to surviving neurons, suggesting that even irreversibly injured neurons are able to mount a protective response. In the Neurotrophins & Receptors PCR array, which consists of genes that are normally expected to be expressed in both groups of hippocampal neurons, only a few genes were expressed at significantly different levels between dying and surviving neurons. Immunohistochemical analysis of selected, differentially expressed proteins supported the gene expression data. This is the first demonstration of pathway-focused PCR array profiling of identified populations of dying and surviving neurons in the brain after TBI. Combining precise laser microdissection of identifiable cells with pathway-focused PCR array analysis is a practical, low-cost alternative to microarrays that provided insight into neuroprotective signals that could be therapeutically targeted to ameliorate TBI-induced neurodegeneration. 相似文献
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Suresh Pallikkuth Luca Micci Zachary S. Ende Robin I. Iriele Barbara Cervasi Benton Lawson Colleen S. McGary Kenneth A. Rogers James G. Else Guido Silvestri Kirk Easley Jacob D. Estes Francois Villinger Savita Pahwa Mirko Paiardini 《PLoS pathogens》2013,9(7)
In pathogenic HIV and SIV infections of humans and rhesus macaques (RMs), preferential depletion of CD4+ Th17 cells correlates with mucosal immune dysfunction and disease progression. Interleukin (IL)-21 promotes differentiation of Th17 cells, long-term maintenance of functional CD8+ T cells, and differentiation of memory B cells and antibody-secreting plasma cells. We hypothesized that administration of IL-21 will improve mucosal function in the context of pathogenic HIV/SIV infections. To test this hypothesis, we infected 12 RMs with SIVmac239 and at day 14 post-infection treated six of them with rhesus rIL-21-IgFc. IL-21-treatment was safe and did not increase plasma viral load or systemic immune activation. Compared to untreated animals, IL-21-treated RMs showed (i) higher expression of perforin and granzyme B in total and SIV-specific CD8+ T cells and (ii) higher levels of intestinal Th17 cells. Remarkably, increased levels of Th17 cells were associated with reduced levels of intestinal T cell proliferation, microbial translocation and systemic activation/inflammation in the chronic infection. In conclusion, IL-21-treatment in SIV-infected RMs improved mucosal immune function through enhanced preservation of Th17 cells. Further preclinical studies of IL-21 may be warranted to test its potential use during chronic infection in conjunction with antiretroviral therapy. 相似文献
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Becker L Kulkarni S Tiwari G Micci MA Pasricha PJ 《American journal of physiology. Gastrointestinal and liver physiology》2012,302(9):G958-G965
Enteric neural stem cells (ENSCs) are a population of neural crest-derived multipotent stem cells present in postnatal gut that may play an important role in regeneration of the enteric nervous system. In most studies, these cells have been isolated from the layer of the gut containing the myenteric plexus. However, a recent report demonstrated that neurosphere-like bodies (NLBs) containing ENSCs could be isolated from mucosal biopsy specimens from children, suggesting that ENSCs are present in multiple layers of the gut. The aim of our study was to assess whether NLBs isolated from layers of gut containing either myenteric or submucosal plexus are equivalent. We divided the mouse small intestine into two layers, one containing myenteric plexus and the other submucosal plexus, and assessed for NLB formation. Differences in NLB density, proliferation, apoptosis, neural crest origin, and phenotype were investigated. NLBs isolated from the myenteric plexus layer were present at a higher density and demonstrated greater proliferation, lower apoptosis, and higher expression of nestin, p75, Sox10, and Ret than those from submucosal plexus. Additionally, they contained a higher percentage of neural crest-derived cells (99.4 ± 1.5 vs. 0.7 ± 1.19% of Wnt1-cre:tdTomato cells; P < 0.0001) and produced more neurons and glial cells than those from submucosal plexus. NLBs from the submucosal plexus layer expressed higher CD34 and produced more smooth muscle-like cells. NLBs from the myenteric plexus layer contain more neural crest-derived ENSCs while those from submucosal plexus appear more heterogeneous, likely containing a population of mesenchymal stem cells. 相似文献
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Micci M. A.; Christensen Burgess N. 《American journal of physiology. Cell physiology》1998,274(6):C1625
The role of theNa+/Ca2+exchanger in intracellular Ca2+regulation was investigated in freshly dissociated catfish retinalhorizontal cells (HC).Ca2+-permeable glutamate receptorsand L-type Ca2+ channels as wellas inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitiveintracellular Ca2+ stores regulateintracellular Ca2+ in these cells.We used the Ca2+-sensitive dyefluo 3 to measure changes in intracellularCa2+ concentration([Ca2+]i)under conditions in whichNa+/Ca2+exchange was altered. In addition, the role of theNa+/Ca2+exchanger in the refilling of the caffeine-sensitiveCa2+ store followingcaffeine-stimulated Ca2+ releasewas assessed. Brief applications of caffeine (1-10 s) producedrapid and transient changes in[Ca2+]i.Repeated applications of caffeine produced smallerCa2+ transients until no furtherCa2+ was released. Store refillingoccurred within 1-2 min and required extracellularCa2+. Ouabain-induced increases inintracellular Na+ concentration([Na+]i)increased both basal free[Ca2+]iand caffeine-stimulated Ca2+release. Reduction of external Na+concentration([Na+]o)further and reversibly increased[Ca2+]iin ouabain-treated HC. This effect was not abolished by the Ca2+ channel blocker nifedipine,suggesting that increases in[Na+]ipromote net extracellular Ca2+influx through aNa+/Ca2+exchanger. Moreover, when[Na+]owas replaced by Li+, caffeine didnot stimulate release of Ca2+ fromthe caffeine-sensitive store afterCa2+ depletion. TheNa+/Ca2+exchanger inhibitor 2',4'-dimethylbenzamil significantlyreduced the caffeine-evoked Ca2+response 1 and 2 min after store depletion. 相似文献