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91.
Ettore Benedetti Benedetto Di Blasio Vincenzo Pavone Carlo Pedone Claudio Toniolo Gian Maria Bonora 《International journal of biological macromolecules》1980,2(4):217-224
The X-ray diffraction and i.r. absorption conformational analysis of has showed the absence of intramolecularly hydrogen-bonded peptide conformations in the solid state. The molecules are held together in rows of ‘cyclic dimer’ motifs through intermolecular NH … OC (acid) and OH … OC {urethane} hydrogen bonds, the secondary amide-like group of the urethane moiety being in the unusual cis conformation, whereas the carboxylic acid group in the common syn conformation. The two molecules in the unit cell present a centrosymmetric set of ?, ψ1, and ψ2 values. In polar solvents solvated species largely predominate. In saturated hydrocarbon solution non-associated and associated (mostly involving the carboxylic acid CO as the proton acceptor) species simultaneously occur. The extent of association decreases with dilution. The amount of intramolecularly hydrogen-bonded oxy-C7 and C5 forms if any, should be extremely small. The type of association at saturation seems to differ from that found in the crystalline compound obtained by precipitation with saturated aliphatic hydrocarbons (from a diethyl ether solution). 相似文献
92.
A new protein factor functional in the ferredoxin-independent light activation of chloroplast fructose 1,6-bisphosphatase 总被引:2,自引:0,他引:2
C Lara A de la Torre B B Buchanan 《Biochemical and biophysical research communications》1980,93(2):544-551
A protein purified from chloroplasts (the “new protein factor”) activated Fru-P2ase in a photochemical reaction that depended only on chloroplast membranes. The results suggest that chloroplasts utilize the newly found mechanism for the photoregulation of Fru-P2ase in addition to the recently described ferredoxin/thioredoxin system. 相似文献
93.
Odile Gateau Maria Rocha de Morillo Pierre Louisot Renée Morelis 《生物化学与生物物理学报:生物膜》1980,595(1):157-160
A sialyl transferase activity is found in purified mitochondria. It is not due to residual contamination and this enzymatic system is located in the outer mitochondrial membrane. This proves mitochondrial autonomy in regard to glycoconjugate sialylation. 相似文献
94.
The F1-ATPase or BF1 factor was purified from Micrococcus lysodeikticus substrain B grown in a synthetic medium in the presence of tritiated amino acids. When analyzed in sodium dodecyl sulfate-7% polyacrylamide gels, the fresh purified preparation contained α, β, γ subunits (referred as the intrinsic subunits) and two other polypeptides (designated as X and component of relative mobility 1.0) whose status as subunits remains to be established. This overall polypeptide composition was similar to that of the F1-ATPase isolated from the same strain grown in complex medium (J. Carreira, J. M. Andreu, M. Nieto, and E. Muñoz., 1976 Mol. Cell. Biochem.10, 67–76). The distribution of 3H-labeled amino acids into purified F1-ATPase and its constituent polypeptides under different stages of growth was used to investigate the biosynthetic relationship between the different polypeptides. The incorporation of amino acids into purified BF1 factor was slower than that of cytoplasmic and other membrane proteins. In isotope-dilution and chase experiments, F1-ATPase showed one of the slowest rates of decay of the incorporated label. These results point out that F1-ATPase of M. lysodeikticus undergoes slower turnover than the overall cytoplasmic and membrane proteins. Pulse and chase experiments allowed us to conclude that the α, β, γ subunits and the components of relative mobility 1.0 are independent with differences in their turnover and therefore do not bear any apparent relation as precursors-products. The two major subunits represent seemingly the “core” of ATPase, the β subunit behaving like the most stable component. On the other hand, the γ subunit appears to be synthesized independently from this α + β complex. 相似文献
95.
Achiléa Lisboa Bittencourt Maria das Graças Santana Araujo Maria do Socorro Fontoura Paes 《Mycopathologia》1980,71(3):155-158
A case of a two-year-old boy with multiple subcutaneous lesions caused by Basidiobolus haptosporus is presented.The child had also a non-toxic familial goiter and clinical and radiological features of a pulmonary illness. The pulmonary manifestations only disappeared with the treatment with potassium iodide. The authors think that the pulmonary lesions must have arisen by direct spread of the fungus from the subcutaneous lesions of the chest. 相似文献
96.
The basic equations for multicomponent transport through partially sieving or leaky membranes are discussed from a statistical-mechanical viewpoint. They have the same mathematical form as the corresponding equations for open membranes, but differ in a discontinuous way from the equations for semipermeable membranes (since a "leak" in a semipermeable membrane constitutes a discontinuous or singular perturbation). Partially sieving membranes can be made to mimic semipermeable behavior through the introduction of characteristic time scales. They may approximate semipermeable behavior at short times, but always deviate at longer times. 相似文献
97.
Summary A 2-year-old girl had a de novo duplication in the long arm of one chromosome 7 and an increased level of the enzyme -glucuronidase in cultured fibroblasts. The phenotype of the girl partly overlaps those of two presumptive syndromes due to secondary partial trisomies 7q. The ratio of the enzyme activity was 1.43 to the controls, and 1.37 to her parent's values. We could not define the abnormality but suggest two alternatives: either the patient is trisomic for region q112 to q22 or for the region q22 to q34. If the second alternative is correct the locus for -glucuronidase is possibly assigned to band 7q22. 相似文献
98.
Maria Cobianu-Bacanu 《Dialectical Anthropology》1977,2(1):301-308
Conclusion These American anthropological studies both reflect and distort our own Romanian-conceptions. Paradoxically, this gives both Romanian and American researchers an opportunity to verify their validity and reliability. The studies are notable for their multiplicity of theoretical approaches to Romanian reality: each author brings in something new by way of interpretation and, at the same time, maintains a dialectical conception as a methodological frame of reference.The authors are certainly conversant with present and future strategies of development in Romania. But their main problem is that they do not always understand the spirit of the latter and consequently fail to interpret contemporary Romanian realities in a deeply humanistic way.And now, some proposals. For a more active dialogue between Romanian and American scientists I suggest a more comprehensive use of the Romanian sources of information, including historical, beletristic, sociological, and political works. With reference to their reflections on history, the American anthropologists could have discussions with Romanian historians, and could use Romanian historical periodization, especially for the contemporary period. For us, the following sequence is basic: the moment of the armed insurrection as the beginning of the popular democratic revolution (August 23, 1944); proclamation of a Peoples' Republic of Romania (December 30, 1947); unification of Social Democratic Party with the Communist Party to form the Romanian Workers Party (Spring 1948); at this time it is possible to speak of a socialist revolution. The analysis of the Americans should take into consideration these important dates in Romanian history and structure their studies accordingly. Sometimes they must pay more attention to the affective connotation of words, especially for a Romanian reader. Such words would be, for example, their use of elite, rather than leaders, bureaucracy rather than administration, pressure rather than social necessities with the consciousness of development, patronclient instead of using interpersonal relations with mutual benefit, etc.I would also urge that substantive discussions between Romanians and Americans begin, built around specific topics of mutual interest, rather than ceremonial meetings which occur when the parties have neither the language skills, data nor social conceptions to interact fruitfully.I suggest to the American researchers that they maintain and strengthen their contacts with Romanian administrators at local, regional and national levels, so that their studies may contribute to our social praxis.Finally, the Americans should always take into account the economic constraints that shape political, juridical, moral, and philosophical phenomena, and try also to grasp more fully the interaction between elements of the superstructure and between superstructure and base in contemporary Romanian socialist society.Above all, along with Professor Cole , I hope that the scientific effort at interpreting Romanian reality, with its lights and shadows, will be used only for reciprocal knowledge and cooperation.Maria Cobianu-Bacanu is Scientific Researcher at the Center for Sociology, Academy of Social and Political Sciences, Bucharest, Romania.
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99.
100.
Chlorine concentrations below 0.8 mg/liter inactivated poliovirus without causing separation of the viral components. These results indicate that the release of RNA from the capsids is the result, not the cause, of virus inactivation by chlorine. 相似文献