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991.
Joseph Tibbs Mohamed Ghoneim Colleen C Caldwell Troy Buzynski Wayne Bowie Elizabeth
M Boehm M
Todd Washington S
M
Ali Tabei Maria Spies 《Nucleic acids research》2021,49(9):e53
Molecular machines within cells dynamically assemble, disassemble and reorganize. Molecular interactions between their components can be observed at the single-molecule level and quantified using colocalization single-molecule spectroscopy, in which individual labeled molecules are seen transiently associating with a surface-tethered partner, or other total internal reflection fluorescence microscopy approaches in which the interactions elicit changes in fluorescence in the labeled surface-tethered partner. When multiple interacting partners can form ternary, quaternary and higher order complexes, the types of spatial and temporal organization of these complexes can be deduced from the order of appearance and reorganization of the components. Time evolution of complex architectures can be followed by changes in the fluorescence behavior in multiple channels. Here, we describe the kinetic event resolving algorithm (KERA), a software tool for organizing and sorting the discretized fluorescent trajectories from a range of single-molecule experiments. KERA organizes the data in groups by transition patterns, and displays exhaustive dwell time data for each interaction sequence. Enumerating and quantifying sequences of molecular interactions provides important information regarding the underlying mechanism of the assembly, dynamics and architecture of the macromolecular complexes. We demonstrate KERA’s utility by analyzing conformational dynamics of two DNA binding proteins: replication protein A and xeroderma pigmentosum complementation group D helicase. 相似文献
992.
Morales-Gamba Ruben Dario de Araújo Maria Lúcia Góes Barcellos Jose Fernando Marcon Jaydione Luiz 《Environmental Biology of Fishes》2021,104(12):1665-1672
Environmental Biology of Fishes - This study evaluated how the plasma steroid hormones testosterone (T) and 17β-estradiol (E2) are related to follicular development in regenerating females of... 相似文献
993.
Meno Laura Escuredo Olga Rodríguez-Flores Maria Shantal Seijo Maria Carmen 《Aerobiologia》2021,37(2):309-320
Aerobiologia - Potatoes production is the main source of income in A Limia (Northwest Spain). Most of the potatoes harvest is stored in piles in farmer´s warehouses without systems to control... 相似文献
994.
Feldmane Daina Druva-Lūsīte Ieva Pole Valentīna Butac Mădălina Maria Militaru Mădălina Missa Imants Meiere Diāna Rubauskis Edgars 《Journal of Plant Growth Regulation》2021,40(2):533-540
Journal of Plant Growth Regulation - Sour cherry ‘Latvijas Zemais’ (Prunus cerasus) is a promising dwarfing rootstock for sweet cherries in Latvia, but low growing rate of newly... 相似文献
995.
Terzin Marko Paletta Maria Grazia Matterson Kenan Coppari Martina Bavestrello Giorgio Abbiati Marco Bo Marzia Costantini Federica 《Coral reefs (Online)》2021,40(4):1391-1391
Coral Reefs - A correction to this paper has been published: https://doi.org/10.1007/s00338-021-02120-y 相似文献
996.
da Silva Fernando Henrique Alves de Morais Patrícia Lígia Dantas da Silva Dias Nildo de Sousa Nunes Glauber Henrique de Morais Marciana Bizerra Melo Marlenildo Ferreira de Albuquerque Nascimento Maria Tereza 《Journal of Plant Growth Regulation》2021,40(3):1298-1314
Journal of Plant Growth Regulation - This study evaluated the effect of saline water irrigation (4.5 dS m−1) on growth, gas exchange and mineral nutrient content in eight melon accessions and... 相似文献
997.
de Sousa Sylvia Morais de Oliveira Christiane Abreu Andrade Daniele Luiz de Carvalho Chainheny Gomes Ribeiro Vitória Palhares Pastina Maria Marta Marriel Ivanildo Evódio de Paula Lana Ubiraci Gomes Gomes Eliane Aparecida 《Journal of Plant Growth Regulation》2021,40(2):867-877
Journal of Plant Growth Regulation - The rising demand for agricultural commodities in developing countries has put increasing pressure on land resources for higher yields, with associated growth... 相似文献
998.
Checchio Mirela Vantini de Cássia Alves Rita de Oliveira Kevein Ruas Moro Gustavo Vitti Santos Durvalina Maria Mathias dos Gratão Priscila Lupino 《Journal of plant research》2021,134(6):1279-1289
Journal of Plant Research - Salinity has become one of the major factors limiting agricultural production. In this regard, different cost-effective management strategies such as the use of plant... 相似文献
999.
Delgado Naranjo Juan Martin Jiménez Callejón Maria José Peñuela Vásquez Mariana Rios Luis Alberto Robles Medina Alfonso 《Journal of applied phycology》2021,33(4):2031-2045
Journal of Applied Phycology - The thraustochytrid Aurantiochytrium limacinum SR21 is a promising source of docosahexaenoic acid (DHA) for human consumption as dietary supplement, replacing... 相似文献
1000.
Murray Kimberley R. Bird Melanie Strack Maria Cody Michael Xu Bin 《Wetlands Ecology and Management》2021,29(2):281-299
Wetlands Ecology and Management - Oil sands exploration activities across the Alberta boreal peatlands requires tree clearing and results in sites being left compressed and with altered understory... 相似文献