全文获取类型
收费全文 | 38493篇 |
免费 | 2486篇 |
国内免费 | 14篇 |
出版年
2023年 | 223篇 |
2022年 | 521篇 |
2021年 | 909篇 |
2020年 | 519篇 |
2019年 | 675篇 |
2018年 | 943篇 |
2017年 | 787篇 |
2016年 | 1318篇 |
2015年 | 1997篇 |
2014年 | 2143篇 |
2013年 | 2934篇 |
2012年 | 3359篇 |
2011年 | 3200篇 |
2010年 | 1956篇 |
2009年 | 1684篇 |
2008年 | 2388篇 |
2007年 | 2321篇 |
2006年 | 2061篇 |
2005年 | 1855篇 |
2004年 | 1742篇 |
2003年 | 1684篇 |
2002年 | 1519篇 |
2001年 | 304篇 |
2000年 | 209篇 |
1999年 | 296篇 |
1998年 | 380篇 |
1997年 | 253篇 |
1996年 | 252篇 |
1995年 | 231篇 |
1994年 | 224篇 |
1993年 | 219篇 |
1992年 | 135篇 |
1991年 | 146篇 |
1990年 | 149篇 |
1989年 | 109篇 |
1988年 | 100篇 |
1987年 | 90篇 |
1986年 | 70篇 |
1985年 | 95篇 |
1984年 | 95篇 |
1983年 | 71篇 |
1982年 | 83篇 |
1981年 | 80篇 |
1980年 | 79篇 |
1979年 | 72篇 |
1978年 | 39篇 |
1977年 | 50篇 |
1976年 | 36篇 |
1975年 | 38篇 |
1973年 | 42篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
961.
962.
Evidence for spontaneous mappings between the dimensions of number and length, time and length, and number and time, has been recently described in preverbal infants. It is unclear, however, whether these abilities reflect the existence of privileged mappings between certain quantitative dimensions, like number, space and time, or instead the existence of a magnitude system underlying the representation of any quantitative dimension, and allowing mappings across those dimensions. Four experiments, using the same methods from previous research that revealed a number-length mapping in eight-month-old infants, investigated whether infants of the same age establish mappings between number and a different, non-spatial continuous dimension: level of brightness. We show that infants are able to learn and productively use mappings between brightness and number when they are positively related, i.e., larger numbers paired with brighter or higher contrast levels, and fail when they are inversely related, i.e., smaller numbers paired with brighter or higher contrast levels, suggesting that they are able to learn this mapping in a specific direction. However, infants not only do not show any baseline preference for any direction of the number-brightness mapping, but fail at transferring the discrimination from one dimension (number) to the other (brightness). Although infants can map multiple dimensions to one another, the number-length mapping may be privileged early in development, as it is for adults. 相似文献
963.
964.
Cynthia Lions Emmanuel Bui-Quoc Sylvette Wiener-Vacher Magali Seassau Maria Pia Bucci 《PloS one》2013,8(12)
Purpose
The objective of our study was to examine horizontal smooth pursuit performance in strabismic children and in children with vergence deficits, and to compare these data with those recorded in a group of control age-matched children.Methods
Binocular eye movements were recorded by video-oculography in ten strabismic children (mean age: 9.8±0.8) and seven children with vergence deficits (mean age: 10.8±0.6). Data were compared to that of age-matched control children (mean age: 9.8±0.8 years).Results
Catch-up saccades amplitude in strabismic children and in children with vergence deficits were significantly higher than in control age-matched children. Moreover, in strabismic children the amplitude of catch-up saccades was significantly higher in rightward than in leftward direction. The number of catch-up saccades was also significantly higher in rightward than in leftward direction. The gain value of pursuits in rightward direction was significantly higher in the right eye than in the left one; for the right eye, the gain value was significantly higher in rightward than in leftward direction. Binocular coordination of pursuit was better in control age-matched children than in children with vergence deficits and than in strabismic children.Conclusions
Binocular coordination of pursuit is abnormal in children with vergence deficits and worse in strabismic children. Binocular vision plays an important role in improving binocular coordination of pursuit. 相似文献965.
André M. Ribeiro-dos-Santos Jorge Estefano Santana de Souza Renan Almeida Dayse O. Alencar Maria Silvanira Barbosa Leonor Gusm?o Wilson A. Silva Jr Sandro J. de Souza Artur Silva ?ndrea Ribeiro-dos-Santos Sylvain Darnet Sidney Santos 《PloS one》2013,8(12)
The emergence of next-generation sequencing technologies allowed access to the vast amounts of information that are contained in the human genome. This information has contributed to the understanding of individual and population-based variability and improved the understanding of the evolutionary history of different human groups. However, the genome of a representative of the Amerindian populations had not been previously sequenced. Thus, the genome of an individual from a South American tribe was completely sequenced to further the understanding of the genetic variability of Amerindians. A total of 36.8 giga base pairs (Gbp) were sequenced and aligned with the human genome. These Gbp corresponded to 95.92% of the human genome with an estimated miscall rate of 0.0035 per sequenced bp. The data obtained from the alignment were used for SNP (single-nucleotide) and INDEL (insertion-deletion) calling, which resulted in the identification of 502,017 polymorphisms, of which 32,275 were potentially new high-confidence SNPs and 33,795 new INDELs, specific of South Native American populations. The authenticity of the sample as a member of the South Native American populations was confirmed through the analysis of the uniparental (maternal and paternal) lineages. The autosomal comparison distinguished the investigated sample from others continental populations and revealed a close relation to the Eastern Asian populations and Aboriginal Australian. Although, the findings did not discard the classical model of America settlement; it brought new insides to the understanding of the human population history. The present study indicates a remarkable genetic variability in human populations that must still be identified and contributes to the understanding of the genetic variability of South Native American populations and of the human populations history. 相似文献
966.
Katrin Kuhls Elisa Cupolillo Soraia O. Silva Carola Schweynoch Mariana C?rtes Boité Maria N. Mello Isabel Mauricio Michael Miles Thierry Wirth Gabriele Sch?nian 《PLoS neglected tropical diseases》2013,7(10)
Background/Objectives: Parasites of the subgenus Leishmania (Viannia) cause varying clinical symptoms ranging from cutaneous leishmaniases (CL) with single or few lesions, disseminated CL (DL) with multiple lesions to disfiguring forms of mucocutaneous leishmaniasis (MCL). In this population genetics study, 37 strains of L. (V.) guyanensis, 63 of L. (V.) braziliensis, four of L. (V.) shawi, six of L. (V.) lainsoni, seven of L. (V.) naiffi, one each of L. (V.) utingensis and L. (V.) lindenbergi, and one L. (V.) lainsoni/L. naiffi hybrid from different endemic foci in Brazil were examined for variation at 15 hyper-variable microsatellite markers.
Methodology/Principal findings: The multilocus microsatellite profiles obtained for the 120 strains were analysed using both model- and distance-based methods. Significant genetic diversity was observed for all L. (Viannia) strains studied. The two cluster analysis approaches identified two principal genetic groups or populations, one consisting of strains of L. (V.) guyanensis from the Amazon region and the other of strains of L. (V.) braziliensis isolated along the Atlantic coast of Brazil. A third group comprised a heterogeneous assembly of species, including other strains of L. braziliensis isolated from the north of Brazil, which were extremely polymorphic. The latter strains seemed to be more closely related to those of L. (V.) shawi, L. (V.) naiffi, and L. (V.) lainsoni, also isolated in northern Brazilian foci. The MLMT approach identified an epidemic clone consisting of 13 strains of L. braziliensis from Minas Gerais, but evidence for recombination was obtained for the populations of L. (V.) braziliensis from the Atlantic coast and for L. (V.) guyanensis.
Conclusions/Significance: Different levels of recombination versus clonality seem to occur within the subgenus L. (Viannia). Though clearly departing from panmixia, sporadic, but long-term sustained recombination might explain the tremendous genetic diversity and limited population structure found for such L. (Viannia) strains. 相似文献
967.
Anna U. Eriksson Christoffer Svensson Andreas H?rnblad Abbas Cheddad Elena Kostromina Maria Eriksson Nils Norlin Antonello Pileggi James Sharpe Fredrik Georgsson Tomas Alanentalo Ulf Ahlgren 《Journal of visualized experiments : JoVE》2013,(71)
By adapting OPT to include the capability of imaging in the near infrared (NIR) spectrum, we here illustrate the possibility to image larger bodies of pancreatic tissue, such as the rat pancreas, and to increase the number of channels (cell types) that may be studied in a single specimen. We further describe the implementation of a number of computational tools that provide: 1/ accurate positioning of a specimen''s (in our case the pancreas) centre of mass (COM) at the axis of rotation (AR)2; 2/ improved algorithms for post-alignment tuning which prevents geometric distortions during the tomographic reconstruction2 and 3/ a protocol for intensity equalization to increase signal to noise ratios in OPT-based BCM determinations3. In addition, we describe a sample holder that minimizes the risk for unintentional movements of the specimen during image acquisition. Together, these protocols enable assessments of BCM distribution and other features, to be performed throughout the volume of intact pancreata or other organs (e.g. in studies of islet transplantation), with a resolution down to the level of individual islets of Langerhans. 相似文献
968.
Wild animals are commonly parasitized by a wide range of helminths. The four major types of helminths are "roundworms" (nematodes), "thorny-headed worms" (acanthocephalans), "flukes" (trematodes), and "tapeworms" (cestodes). The optimum method for collecting helminths is to examine a host that has been dead less than 4-6 hr since most helminths will still be alive. A thorough necropsy should be conducted and all major organs examined. Organs are washed over a 106 μm sieve under running water and contents examined under a stereo microscope. All helminths are counted and a representative number are fixed (either in 70% ethanol, 10% buffered formalin, or alcohol-formalin-acetic acid). For species identification, helminths are either cleared in lactophenol (nematodes and small acanthocephalans) or stained (trematodes, cestodes, and large acanthocephalans) using Harris'' hematoxylin or Semichon''s carmine. Helminths are keyed to species by examining different structures (e.g. male spicules in nematodes or the rostellum in cestodes). The protocols outlined here can be applied to any vertebrate animal. They require some expertise on recognizing the different organs and being able to differentiate helminths from other tissue debris or gut contents. Collection, preservation, and staining are straightforward techniques that require minimal equipment and reagents. Taxonomic identification, especially to species, can be very time consuming and might require the submission of specimens to an expert or DNA analysis. 相似文献
969.
970.
Alex Fernando de Almeida Sâmia Maria Taulk-Tornisielo Eleonora Cano Carmona 《Annals of microbiology》2013,63(4):1225-1234
Microorganisms can produce lipases with different biochemical characteristics making necessary the screening of new lipase-producing strains for different industrial applications. In this study, 90 microbial strains were screened as potential lipase producers using a sensitive agar plate method with a suitable medium supplemented with Tween 20 and also a liquid culture supplemented with olive oil. The highest cell growth and lipase production for Candida viswanathii were observed in triolein and oleic acid when used as the only pure carbon source. Renewable low-cost triacylglycerols supported the best cell growth, and olive oil was found to be the best inducer for lipase production (19.50 g/L and 58.50 U). The selected conditions for enzyme production were found with yeast extract as nitrogen source and 1.5 % (w/v) olive oil (85.70 U) that resulted in a good cell growth yield (YX/S?=?1.234 g/g) and lipase productivity (1.204 U/h) after 72 h of shake-flask cultivation. C. viswanathii lipase presented high hydrolytic activity on esters bonds of triacylglycerols of long-chain, and this strain can be considered an important candidate for future applications in chemical industries. 相似文献