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991.
992.
993.
Cong-Ping Tan Fang-Qing Zhao Zhong-Liang Su Cheng-Wei Liang Song Qin 《Journal of applied phycology》2007,19(4):347-355
A carotenoid gene (crtR-B) from the green alga Haematococcus pluvialis, encoding β-carotene hydroxylase that was able to catalyze the conversion of β-carotene to zeaxanthin and canthaxanthin to
astaxanthin, was cloned into Chlamydomonas reinhardtii chloroplast expression vector p64D to yield plasmid p64DcrtR-B. The vector p64DcrtR-B was transferred to the chloroplast
genome of C. reinhardtii using micro-particle bombardment. PCR and Southern blot analyses indicated that crtR-B was integrated into the chloroplast genome of the transformants. RT-PCR assays showed that the H. pluvialis
crt R-B gene was expressed in C. reinhardtii transformants. The transformants rapidly synthesized carotenoids in larger quantities than the wild-type upon being transferred
from moderate to high-intensity white light. This research provides a foundation for further study to elucidate the possible
mechanism of photo-protection by xanthophylls and other carotenoids in high light conditions or through exposure to UV radiation. 相似文献
994.
Flocculation is an eco-friendly process of cell separation, which has been traditionally exploited by the brewing industry.
Cell surface charge (CSC), cell surface hydrophobicity (CSH) and the presence of active flocculins, during the growth of two
(NCYC 1195 and NCYC 1214) ale brewing flocculent strains, belonging to the NewFlo phenotype, were examined. Ale strains, in
exponential phase of growth, were not flocculent and did not present active flocculent lectins on the cell surface; in contrast,
the same strains, in stationary phase of growth, were highly flocculent (>98%) and presented a hydrophobicity of approximately
three to seven times higher than in exponential phase. No relationship between growth phase, flocculation and CSC was observed.
For comparative purposes, a constitutively flocculent strain (S646-1B) and its isogenic non-flocculent strain (S646-8D) were
also used. The treatment of ale brewing and S646-1B strains with pronase E originated a loss of flocculation and a strong
reduction of CSH; S646-1B pronase E-treated cells displayed a similar CSH as the non-treated S646-8D cells. The treatment
of the S646-8D strain with protease did not reduce CSH. In conclusion, the increase of CSH observed at the onset of flocculation
of ale strains is a consequence of the presence of flocculins on the yeast cell surface and not the cause of yeast flocculation.
CSH and CSC play a minor role in the auto-aggregation of the ale strains since the degree of flocculation is defined, primarily,
by the presence of active flocculins on the yeast cell wall. 相似文献
995.
Laura Borello Mario Ferraro Piero Penengo Maria Lia Rossotti 《Biological cybernetics》1981,39(2):79-85
In this paper we propose a model of visual perception in which a positive feedback mechanism can reproduce the pattern stimulus on a neurons screen. The pattern stimulus reproduction is based on informations coming from the spatial derivatives of visual pattern. This information together with the response of the feature extractors provides to the reproduction of the visual pattern as neuron screen electric activity. We simulate several input patterns and prove that the model reproduces the percept. 相似文献
996.
Background
Influenza pandemic remains a serious threat to human health. Viruses of avian origin, H5N1, H7N7 and H9N2, have repeatedly crossed the species barrier to infect humans. Recently, a novel strain originated from swine has evolved to a pandemic. This study aims at improving our understanding on the pathogenic mechanism of influenza viruses, in particular the role of non-structural (NS1) protein in inducing pro-inflammatory and apoptotic responses.Methods
Human lung epithelial cells (NCI-H292) was used as an in-vitro model to study cytokine/chemokine production and apoptosis induced by transfection of NS1 mRNA encoded by seven infleunza subtypes (seasonal and pandemic H1, H2, H3, H5, H7, and H9), respectively.Results
The results showed that CXCL-10/IP10 was most prominently induced (> 1000 folds) and IL-6 was slightly induced (< 10 folds) by all subtypes. A subtype-dependent pattern was observed for CCL-2/MCP-1, CCL3/MIP-1α, CCL-5/RANTES and CXCL-9/MIG; where induction by H5N1 was much higher than all other subtypes examined. All subtypes induced a similar temporal profile of apoptosis following transfection. The level of apoptosis induced by H5N1 was remarkably higher than all others. The cytokine/chemokine and apoptosis inducing ability of the 2009 pandemic H1N1 was similar to previous seasonal strains.Conclusions
In conclusion, the NS1 protein encoded by H5N1 carries a remarkably different property as compared to other avian and human subtypes, and is one of the keys to its high pathogenicity. NCI-H292 cells system proves to be a good in-vitro model to delineate the property of NS1 proteins.997.
Baozhu Guo Xiaoping Chen Phat Dang Brian T Scully Xuanqiang Liang C Corley Holbrook Jiujiang Yu Albert K Culbreath 《BMC developmental biology》2008,8(1):12
Background
Peanut (Arachis hypogaea L.) is an important crop economically and nutritionally, and is one of the most susceptible host crops to colonization of Aspergillus parasiticus and subsequent aflatoxin contamination. Knowledge from molecular genetic studies could help to devise strategies in alleviating this problem; however, few peanut DNA sequences are available in the public database. In order to understand the molecular basis of host resistance to aflatoxin contamination, a large-scale project was conducted to generate expressed sequence tags (ESTs) from developing seeds to identify resistance-related genes involved in defense response against Aspergillus infection and subsequent aflatoxin contamination. 相似文献998.
999.
Background
Insertions and deletions (indels) are an important evolutionary force, making the evolutionary process more efficient and flexible by copying and removing genomic fragments of various lengths instead of rediscovering them by point mutations. As a mutational process, indels are known to be more active in specific sequences (like micro-satellites) but not much is known about the more general and mechanistic effect of sequence context on the insertion and deletion susceptibility of genomic loci. 相似文献1000.
A survey of blood selenium (Se) concentrations in Norwegian Red heifers and dry period cows was conducted to reveal possible
association to management, feeding, health and fertility. Selenium contents were determined in 254 herd blood samples consisting
of pooled samples from individual non-lactating animals from herds in 5 counties. The Se concentrations showed a normal distribution
with mean 0.09 μg Se/g blood, with a standard deviation (SD) of 0.05, and ranged from 0.02 to 0.23 μg/g, with 50 % of the
samples being between 0.06 and 0.11 μg/g. The herds with Se concentrations below 0.06 μg/g were smaller (21.4 ± 8.7 cow-years)
than those with Se levels above 0.11 μg/g (27.5 ± 14.1 cow-years) (P < 0.01), but there were no differences in milk yield, incidence of replacement, proportion of animal culling, amount of concentrate
or grass silage as percentage of energy consumption between the groups. Treatment registration records showed a tendency that
more animals in the low Se herds were treated for all the diseases included in this investigation (64.8 animals per 100 cow-years)
than those in the high Se herds (57.5 per 100 cow-years), while no such differences were revealed for individual disorders.
There was, however, a significant difference in bulk milk somatic cell counts (BMSCC) between low and high Se herds, their
values being 137 000 and 155 000 cells/ml, respectively. This difference was significantly influenced by herd size. Furthermore,
a total of 4 916 lactations were analyzed from individual health and fertility recordings, including 2 934 first lactations
and 1 982 later lactations. The present study revealed a reduced incidence of disease treatment with increased Se concentrations
from 0.02 to 0.23 μg Se/g blood. In this regard, there seemed to be an optimum of 0.10 to 0.15 μg Se/g for all types of mastitis
treatments summarized, and for treatment of retained placenta. Thus, herd Se concentrations below and above these values was
connected with increased probability for sum mastitis and retained placenta, reflecting the effect of the quadratic term of
Se. The cow (composite) milk somatic cell count (SCC) was lower in lactations from low Se herds than in high Se herds with
a marked SCC increase in the Se concentration interval from 0.11–0.13 μg/g blood. In conclusion, heifers and dry period cows
in Norway are low in blood Se content and there seems to be a positive association between increased blood Se concentration
pre partum and decreased incidence of mastitis, ovarian cysts and anoestrus/silent oestrus post partum. 相似文献