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121.
122.
Margot Schulz O. Traub Mona Knop K. Willecke Heide Schnabl 《Plant biology (Stuttgart, Germany)》1992,105(2):111-115
Mesophyll protoplasts from three week old leaves of Helianthus annuus L. and from four week old leaves of Vicia faba L. were incubated with polyclonal, monospecific antibodies, raised against either cx 32 or cx 26 mouse liver connexin. Crossreactions were visualized by FITC-labeled anti-rabbit antibodies. Incubations with the cx 26 antibody resulted in fluorescing spots on protoplast surfaces of both plant species, indicating the presence of a polypeptide, immunologically related to the animal cx 26. A plant protein, exhibiting similarities to cx 26, would present a new member of connexin-like plant proteins. Controls, performed with preimmune serum or with the FITC-conjugate alone, were negative. Immunofluorescing spots were not obtained after incubations with the cx 32 antibody. Since the existence of a cx 32-like plant protein, associated with ultrastructures of plasmodesmata and the plasma membrane, is meanwhile established, several explanations for the failed attempt to demonstrate a cx 32 antibody labeling at protoplast surfaces are discussed. 相似文献
123.
Wild tomato endosperm transcriptomes reveal common roles of genomic imprinting in both nuclear and cellular endosperm
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Morgane Roth Ana M. Florez‐Rueda Margot Paris Thomas Städler 《The Plant journal : for cell and molecular biology》2018,95(6):1084-1101
Genomic imprinting is a conspicuous feature of the endosperm, a triploid tissue nurturing the embryo and synchronizing angiosperm seed development. An unknown subset of imprinted genes (IGs) is critical for successful seed development and should have highly conserved functions. Recent genome‐wide studies have found limited conservation of IGs among distantly related species, but there is a paucity of data from closely related lineages. Moreover, most studies focused on model plants with nuclear endosperm development, and comparisons with properties of IGs in cellular‐type endosperm development are lacking. Using laser‐assisted microdissection, we characterized parent‐specific expression in the cellular endosperm of three wild tomato lineages (Solanum section Lycopersicon). We identified 1025 candidate IGs and 167 with putative homologs previously identified as imprinted in distantly related taxa with nuclear‐type endosperm. Forty‐two maternally expressed genes (MEGs) and 17 paternally expressed genes (PEGs) exhibited conserved imprinting status across all three lineages, but differences in power to assess imprinted expression imply that the actual degree of conservation might be higher than that directly estimated (20.7% for PEGs and 10.4% for MEGs). Regardless, the level of shared imprinting status was higher for PEGs than for MEGs, indicating dissimilar evolutionary trajectories. Expression‐level data suggest distinct epigenetic modulation of MEGs and PEGs, and gene ontology analyses revealed MEGs and PEGs to be enriched for different functions. Importantly, our data provide evidence that MEGs and PEGs interact in modulating both gene expression and the endosperm cell cycle, and uncovered conserved cellular functions of IGs uniting taxa with cellular‐ and nuclear‐type endosperm. 相似文献
124.
Tricia L. May-Dracka Robert Arduini Andrea Bertolotti-Ciarlet Govinda Bhisetti Margot Brickelmaier Ellen Cahir-McFarland Istvan Enyedy Jason D. Fontenot Thomas Hesson Kevin Little Joe Lyssikatos Douglas Marcotte Timothy McKee Paramasivam Murugan Thomas Patterson Hairuo Peng Mia Rushe Laura Silvian Linda C. Burkly 《Bioorganic & medicinal chemistry letters》2018,28(10):1964-1971
Germinal center kinase-like kinase (GLK, also known as MAP4K3) has been hypothesized to have an effect on key cellular activities, including inflammatory responses. GLK is required for activation of protein kinase C-θ (PKCθ) in T cells. Controlling the activity of T helper cell responses could be valuable for the treatment of autoimmune diseases. This approach circumvents previous unsuccessful approaches to target PKCθ directly. The use of structure based drug design, aided by the first crystal structure of GLK, led to the discovery of several inhibitors that demonstrate potent inhibition of GLK biochemically and in relevant cell lines. 相似文献
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Margot Lauritzen 《Molecular & general genetics : MGG》1953,85(2):220-237
Ohne ZusammenfassungMit 2 Textabbildungen.Auszug aus einer Dissertation der Naturwissenschaftlich-mathematischen Fakultät der Albert-Ludwigs-Universität Freiburg i. Br. Ich danke Herrn Prof. Oehlkers für die Überlassung des Themas, Frau Prof. Harte für ihre Mithilfe und Prof. Rudorf für die Möglichkeit, in Müncheberg meine Kulturen anbauen zu können. 相似文献
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129.
Philippe Margot Michael Wahlen Ahmad Gholamhuseinian Patrick Piggot Dimitri Karamata 《Journal of bacteriology》1998,180(3):749-752
Bacillus subtilis cell wall-bound protein CWBP33 is encoded by lytE, a gene expressed during the exponential growth phase. Sequence analysis of LytE, a 33-kDa protein, reveals two domains. The N-terminal domain contains a threefold-repeated motif common to several peptidoglycan binding proteins, while the C-terminal domain, probably carrying the catalytic activity, has homology with certain exoproteins. Zymographs unambiguously reveal that the absence of CWBP33, due to inactivation of lytE, is accompanied by the loss of a lytic activity. In lytE mutants, the cell autolysis rate is significantly decreased, although autolysis of corresponding, purified cell walls does not seem to be affected. 相似文献
130.
Chezian Somasundaram Robert Arch Siegfried Matzku Margot Zöller 《Cancer immunology, immunotherapy : CII》1996,42(6):343-350
A bispecific F(ab′)2 antibody conjugate (BAC) was constructed against the complement receptor CR3 of macrophages and a variant CD44 (CD44v6) antigen
of rat pancreatic adenocarcinoma cells to redirect macrophage-mediated tumor cytotoxicity. The Fab′ fragments of monoclonal
antibodies (mAb) 1.1ASML and OX42, recognizing the CD44v6 and the CR3 antigens respectively, were chemically coupled at the
hinge region using 5,5′-dithiobis(2-nitrobenzoate). The BAC was characterized in vitro for its specific, dual binding capacity
to CD44v6 and CR3 antigens. Although the monovalence of the BAC resulted in lower avidities to both the antigens as expected,
it was still able to form stable cross-linkages between tumor cells and macrophages in culture leading to the formation of
“clump-like” cell aggregates. The in vitro and in vivo tumor-targeting capacity of the BAC was compared with that of the parental
antitumor mAb 1.1ASML, which mediates tumor killing by antibody-dependent cell cytotoxicity. These results showed that, even
though the bivalent mAb 1.1ASML did not mediate stable cross-linking of target and effector cells, its Fc-receptor-mediated
killing of tumor cells was more effective when compared to the BAC. Thus, this study strongly supports the hypothesis that
firm persistent binding between effector and target cells per se is not as important as the choice of trigger molecule used for macrophage activation to redirect their tumor cytotoxic potential
effectively.
Received: 2 May 1996 / Accepted: 21 May 1996 相似文献