Review: SMCs in the World of Chromosome Biology— From Prokaryotes to Higher Eukaryotes

The study of higher order chromosome structure and how it is modified through the course of the cell cycle has fascinated geneticists, biochemists, and cell biologists for decades. The results from many diverse technical avenues have converged in the discovery of a large superfamily of chromosome-associated proteins known as SMCs, for structural maintenance of chromosomes, which are predicted to have ATPase activity. Now found in all eukaryotes examined, and numerous prokaryotes as well, SMCs play crucial roles in chromatid cohesion, chromosome condensation, sex chromosome dosage compensation, and DNA recombination repair. In eukaryotes, SMCs exist in five subfamilies, which appear to associate with one another in particular pairs to perform their specific functions. In this review, we summarize current progress examining the roles these proteins, and the complexes they form, play in chromosome metabolism. We also present a twist in the SMC story, with the possibility of one SMC moonlighting in an unpredicted location.     

Developmental study of neuropeptide Y-like immunoreactivity in the neurohypophysis and intermediate lobe of the rhesus monkey (Macaca mulatta)

Summary The purpose of this study was to examine the development and distribution of neuropeptide Y-immunoreactive fibers in the neurohypophysis of the rhesus monkey (Macaca mulatta) throughout life and the relationship of these fibers to the hypothalamo-hypophyseal portal vasculature. In rhesus monkeys, which varied in age from fetal life to 34 years, neuropeptide Y-immunoreactive fibers were present at all ages examined. In adult monkeys, varicose neuropeptide Y-labeled fibers were concentrated in the upper infundibular stem in association with capillary loops of the portal vasculature and the long portal vessels. Other fibers travelled down the infundibular stem and were distributed at the junction of the lower infundibular stem and infundibular process in the vicinity of the short portal vessels. In the infundibular process, neuropeptide Y-immunoreactive fibers were concentrated along the border of the intermediate lobe. Other stained fibers were sparsely distributed in the infundibular process and were often associated with small vessels. Neuropeptide Y-immunoreactivity was also located in a few fibers and cells of the intermediate lobe. Very few labeled fibers were seen in the fetal neurohypophysis, but their number increased gradually during the first postnatal year. At two years of age, a high density of stained fibers was observed, especially in the infundibular process. The number of axons in the infundibular process was lower at 12 years and continued to decline until 34 years of age. Neuropeptide Y may modulate hormone release at these sites and may also be released directly into vessels in the infundibular process. The close association of neuropeptide Y-labeled fibers with capillaries of the portal vasculature strongly suggests that neuropeptide Y is released into the portal blood of monkeys throughout life and may influence hormone secretion from the anterior pituitary gland.     

Human autoantibody to topoisomerase II

The rheumatic diseases are characterized by the production of autoantibodies that are usually directed against components of the cell nucleus. In this communication, we describe autoantibodies that recognize DNA topoisomerase II (anti-topoII) present in the serum of a patient with systemic lupus erythematosus. Several lines of evidence indicate that this antibody recognizes topoisomerase II. First, it binds to the native enzyme in soluble extracts prepared from isolated chromosomes and effectively depletes such extracts of active enzyme. Second, the serum binds to topoisomerase II in immunoblots of mitotic chromosomes and chromosome scaffolds. Finally, the antiserum binds strongly to a fusion protein encoded by a cloned cDNA and expressed in Escherichia coli that (based on immunological evidence) represents the carboxy-terminal portion of chicken topoisomerase II. Autoantibodies such as the one described here may provide useful reagents for the study of human topoisomerase II.     

Entwicklungsgeschichtlich-genetische Untersuchungen an Epilobium

Zusammenfassung Es wird der Erbgang einesEpilobium hirsutum status albomaculatus beschrieben. Trotz einer normalerweise mütterlichen Vererbung treten bei der Bestäubung (normal grün) x (albomaculata weiß) infolge Übertritt von Plastiden aus dem Pollenschlauch in die Eizelle 0,22% gescheckte Pflanzen auf. In annähernd derselben Häufigkeit kommen unter den sterilenEpilobium-Pflanzen, die einenhirsutum-Kern imluteum-Plasma enthalten (Lhⁿ) Individuen vor, die einzelne fertile Äste oder Blüten tragen. Die Verteilung dieser fertilen Blüten erinnert an die Verteilung weißen Gewebes bei den Schecken. Da der verschiedene Fertilitätsgrad dieser Blüten einer Pflanze auf die Nachkommen mütterlicherseits vererbt wird, so wird angenommen, daß die Fertilitätssteigerung durch Übertritt des dem Kernanteil entsprechendenhirsutum-Plasmas bedingt ist.In früheren Versuchen wurde an der Kreuzung:Lhⁿ xluteum festgestellt, daß unter dem Einfluß des eingekreuzten, artfremdenhirsutum-Kernes das Plasma vonEpilobium luteum um ein Geringes dem vonhirsutum ähnlicher geworden ist. Dieser Vorgang kann nicht durch eine Beimischung arteigenen Plasmas während der Rückkreuzung (Lhⁿ x h ) entstehen, da bei der Kreuzung wahrscheinlichhirsutum-Plasma enthaltender fertiler Blüten mitluteum die Plasmaänderung in weit geringerem Maße festzustellen ist, als bei der Kreuzung steriler Blüten mitluteum. Die Annahme einer Umprägung des Erbträgers: Plasma durch einen artfremden eingekreuzten Kern besteht demnach zu Recht. Anhangsweise wird erörtert, auf welchen Vorgängen im Plasma eine derartige Dauermodifikation beruhen kann.Mit 10 Textabbildungen.Mit Unterstützung der Notgemeinschaft der Deutschen Wissenschaft.     

Pericentromeric Satellite III transcripts induce etoposide resistance

Non-coding RNA from pericentromeric satellite repeats are involved in stress-dependent splicing processes, maintenance of heterochromatin, and are required to protect genome stability. Here we show that the long non-coding satellite III RNA (SatIII) generates resistance against the topoisomerase IIa (TOP2A) inhibitor etoposide in lung cancer. Because heat shock conditions (HS) protect cells against the toxicity of etoposide, and SatIII is significantly induced under HS, we hypothesized that the protective effect could be traced back to SatIII. Using genome methylation profiles of patient-derived xenograft mouse models we show that the epigenetic modification of the SatIII DNA locus and the resulting SatIII expression predict chemotherapy resistance. In response to stress, SatIII recruits TOP2A to nuclear stress bodies, which protects TOP2A from a complex formation with etoposide and results in decreased DNA damage after treatment. We show that BRD4 inhibitors reduce the expression of SatIII, restoring etoposide sensitivity.Subject terms: Cancer therapeutic resistance, Epigenetics, Long non-coding RNAs     

Karyotype of Callithrix mauesi (Callitrichidae,Primates) and its relations with those of C. emiliae and C. jacchus

A study was conducted on the most recently described marmoset species, Callithrix mauesi, and the results obtained were compared to those previously reported for the karyotypes of C. jacchus and C. emiliae. No mechanism of chromosome rearrangement differentiates the karyotypes of C. mauesi (2n = 44) and C. emiliae (2n = 44), which diverge from C. jacchus (2n = 46) by a Robertsonian translocation and a paracentric inversion. C. mauesi, like C. emiliae, presents telomeric constitutive heterochromatin in various chromosomes, forming large heterochromatic blocks in some. This does not occur in C. jacchus, which basically presents centromeric constitutive heterochromatin. The karyotype of C. mauesi differs from that of C. emiliae only by the amount and distribution of this telomeric constitutive heterochromatin. One of the chromosomes presenting a heterochromatic block in C. mauesi is chromosome X, a fact not previously reported in the Order Primates. The present chromosome data show that C. mauesi is closer to C. emiliae than to C. jacchus, in agreement with its inclusion in the C. argentata group. In the present paper, we describe for the first time, at the chromosome level, chimerism between fraternal twins of the same sex (XY/XY), with the heterochromatic block of pair 2 being the marker. © 1994 Wiley-Liss, Inc.