Stimulation of poly-β-hydroxybutyrate oxidation in Sphaerotilus discophorus by manganese and magnesium

Summary When grown with glucose, S. discophorus synthesized large amounts of poly--hydroxybutyrate which accumulated intracellularly as sudanophilic granules. The rate of endogenous oxygen consumption by such cells was markedly increased by Mn⁺⁺ and even more by Mg⁺⁺. It has been shown that these inorganic ions stimulate the oxidation of the intracellular poly--hydroxybutyrate.Dedicated by the senior author to Prof. C. B. van Niel on the occasion of his 70th birthday with gratitude for many unforgettable years of association, instruction and stimulation.     

HYBRIDS AND GENOME RELATIONS OF HIBISCUS SABDARIFFA,H. MEEUSEI,H. RADIATUS AND H. ACETOSELLA

Chromosome pairing was studied in the following hybrids: Hibiscus radiatus-meeusei (tetraploid F₁), H. sabdariffa-meeusei (tetraploid F₁ and spontaneous allooctoploid F₂), and hexaploid H. acetosella-(sabdariffa-meeusei). Genome constitutions of the species adduced from these data are symbolized as follows: H. radiatus and H. acetosella, AABB; H. meeusei, AAXX; H.sabdariffa, XXYY or AAYY.     

Treatment of Basal Cell Carcinoma by Curettage and Electrosurgery

One hundred and forty-eight basal cell carcinomas were treated by curettage and electrosurgery. Twenty-six recurrent carcinomas were treated and 24 did not recur during a minimum two-year follow-up. Seventy-two newly diagnosed carcinomas were treated by the same method, and a two-year recurrence-free rate of 97.4% was obtained. About 50 new and recurrent lesions were treated in three patients in whom extensive cutaneous changes from actinic atrophy and previous therapy made the distinction between new and recurrent lesions difficult or impossible. This technique has a particular place in the management of multiple lesions. Patient acceptance is good. Lesions at some sites, e.g. on the nose, where closure of a wound is difficult, are better managed by this method than by surgical excision. Secondary infection is rare and the cosmetic results are excellent.     

Physical mapping of the von Recklinghausen neurofibromatosis region on chromosome 17

The von Recklinghausen neurofibromatosis (NF1) locus has been linked to chromosome 17, and recent linkage analyses place the gene on the proximal long arm. NF1 probably resides in 17q11.2, since two unrelated NF1 patients have been identified who possess constitutional reciprocal translocations involving 17q11.2 with chromosomes 1 and 22. We have used a somatic-cell hybrid from the t(17;22) individual, along with other hybrid cell lines, to order probes around the NF1 locus. An additional probe, 17L1, has been isolated from a NotI linking library made from flow-sorted chromosome 17 material and has been mapped to a region immediately proximal to the translocation breakpoint. While neither NF1 translocation breakpoint has yet been identified by pulse-field gel analysis, an overlap between two probes, EW206 and EW207, has been detected. Furthermore, we have identified the breakpoint in a non-NF1 translocation, SP-3, on the proximal side of the NF1 locus. This breakpoint has been helpful in creating a 1,000-kb pulsed-field map, which includes the closely linked NF1 probes HHH202 and TH17.19. The combined somatic-cell hybrid and pulsed-field gel analysis we report here favors the probe order D17Z1-HHH202-TH17.19-CRYB1-17L1-NF1- (EW206, EW207, EW203, L581, L946)-(ERBB2, ERBA1). The agreement in probe ordering between linkage analysis and physical mapping is excellent, and the availability of translocation breakpoints in NF1 should now greatly assist the cloning of this locus.     

On being ethnic: The politics of identity breaking and making in Canada,or, Nevra on Sunday

The creation of ethnically sensitive health care is a major federal and provincial government concern in Canada at present. The concept of multiculturalism is used to reinforce the notion of rights for minority groups and the Canadian mozaic is explicitly contrasted with the American melting pot. In this paper, the lives of Greek immigrant women in Montreal are used to illustrate how class and gender are as relevant to the immigrant experience as is ethnicity. It is shown how values which were central to female identity in Greece can become a liability after immigration and how the notion of Greek identity in Canada is a fluid category which is subject to repeated transformations. It is suggested that medical anthropologists who ignore the complexity of social categories and whose focus is limited to the cultural construction of illness and the expression of distress are in danger of reinforcing a notion of the quaint ethnic, a stereotype to which the concept of multiculturalism is often reduced.

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Résumé La création de services de santé sensibles aux besoins des différents groupes ethniques est présentement un socci majeur des gouvernements fédéral et provincial au Canada. Le concept de multiculturalisme est utilisé pour renforcer I'idée des droits des groupes minoritaires et la mosaïque canadienne est explicitement contrastée avec the American melting pot. Dans cet exposé, j'utilise la vie des femmes immigrantes grecques à Montréal pour démontrer que la classe et le sexe sont aussi pertinent par rapport à l'expérience des immigrants que l'ethnicité. On montre de quelle façon les valeurs qui sont centrales à l'identité féminine en Grèce peuvent en venir à constituer un problème après l'immigration et comment la notion d'identité grecque au Canada est une catégorie fluide qui est sujette à de multiples transformations. On suggère que les anthropologues médicaux qui ne tiennent pas compte de la complexité des catégories sociales et dont le centre d'intérêt est limité à la cultural construction of illness et à l'expression of distress courent le danger de renforcer la notion de quaint ethnic, un stéréotype auquel le concept de multiculturalisme se trouve souvent réduit. Notes 1 See, for example, Low 1985; Davis 1989; Dunk 1989; Guarnaccia et al. 1989; Van Schaik 1989. 2 Data for this study were obtained initially from eighty-three fast generation Greek immigrant women living in Montreal who were given semi-structured interviews in Greek in their homes. It became apparent in the course of carrying out these interviews that the concept of nevra was central in the narratives given by virtually all of the respondents. This term was inevitably used to link environmental and psychosocial variables to distress and painful physical states. In the second phase of the research, 19 of the original women were selected for in-depth open-ended interviews during which the concept of nevra was discussed in detail. A further data set was obtained from twenty five women who were interviewed in the office of a Greek Canadian general practitioner and 15 more attending a poly-clinic in a Montreal teaching hospital. These interviews were carried out in order to establish if nevra is used as a presenting complaint in clinical settings, and if so, how it is managed by health care professionals. Several internists, psychiatrists, family and general practitioners, and social workers who have daily contact with Greek patients were also interviewed.

     

Competition of cyclooctenes and cyclooctadienes for ethylene binding and activity in plants

trans-Cyclooctene, cis,trans-1,5-cyclooctadiene, and cis,trans-1,3-cyclooctadiene have been compared with the cis and cis,cis isomers and with 2,5-norbornadiene for competition with ethylene for binding in mung bean sprouts and tobacco and for action (induction of chlorophyll degradation) in banana. The compounds containing a trans double bond were much more effective in competition for binding and action than the cis and cis,cis compounds. trans-Cyclooctene and cis,trans-1,3-cyclooctadiene were in the general range of 50–90 times more effective than 2,5-norbornadiene.R.J. Reynolds Research Apprentice