全文获取类型
收费全文 | 5526篇 |
免费 | 338篇 |
国内免费 | 2篇 |
出版年
2023年 | 42篇 |
2022年 | 78篇 |
2021年 | 161篇 |
2020年 | 127篇 |
2019年 | 133篇 |
2018年 | 147篇 |
2017年 | 148篇 |
2016年 | 260篇 |
2015年 | 294篇 |
2014年 | 319篇 |
2013年 | 421篇 |
2012年 | 491篇 |
2011年 | 460篇 |
2010年 | 304篇 |
2009年 | 242篇 |
2008年 | 325篇 |
2007年 | 337篇 |
2006年 | 286篇 |
2005年 | 246篇 |
2004年 | 200篇 |
2003年 | 190篇 |
2002年 | 166篇 |
2001年 | 65篇 |
2000年 | 64篇 |
1999年 | 53篇 |
1998年 | 47篇 |
1997年 | 34篇 |
1996年 | 24篇 |
1995年 | 28篇 |
1994年 | 20篇 |
1993年 | 16篇 |
1992年 | 15篇 |
1991年 | 13篇 |
1990年 | 17篇 |
1989年 | 14篇 |
1988年 | 13篇 |
1987年 | 10篇 |
1986年 | 6篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 7篇 |
1982年 | 2篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1968年 | 2篇 |
1959年 | 2篇 |
排序方式: 共有5866条查询结果,搜索用时 31 毫秒
991.
Marcelo Piaia Carolina Bonet Bub Guilherme de Menezes Succi Margareth Torres Thiago Henrique Costa Fabricio Costa Pinheiro Marcelo Henrique Napimoga 《Cell and tissue banking》2017,18(1):75-81
According to the Brazilian Association of Organ Transplants, in 2015, 19,408 bone transplants were performed in Brazil, over 90% by Dental Surgeons. The surgical technique itself has a respectable number of reports regarding its clinical efficacy, as measured by long-term survival of dental implants in grafted areas. Uncertainty remains, however, as to whether fresh frozen grafts from human bone donors remain immunologically innocuous in the body of the host. Six male with no previous medical history of note, including systemic diseases, surgery or blood transfusion were selected. These patients underwent reconstructive procedures (sinus lifting) using fresh frozen human bone from a tissue bank. All patients had venous blood samples collected prior to surgery and 6 months after the procedure. Anti-HLA analysis for the detection of HLA (human leukocyte antigen) antibodies was performed using methods such as the LABScreen PRA Class I and Class II, LABScreen Single Antigen Class I and Class II, Luminex Platform. Reactive individuals to the screening tests (LABScreen PRA) were further investigated to determine the specificity of the antibodies detected (LABScreen Single Antigen) with a cutoff value of median fluorescence intensity ≥500. As a result, it was observed that two patients (33%) were positive in screening tests, one presenting with anti-HLA Class I and II sensitization and the other with anti-HLA class II. The specificity analysis showed that the patients sensitized to HLA class II presented 4 specificities, 3 of which immunologically relevant. In the second individual, 23 specificities were identified, 6 of which immunologically important for HLA class I and 4 specificities for HLA class II, 3 of these were immunologically important. All specificities detected had average fluorescence. These findings are suggestive that sinus-lifting procedures with allogeneic bone can induce immunological sensitization. 相似文献
992.
Transgenic cotton expressing Cry10Aa toxin confers high resistance to the cotton boll weevil
下载免费PDF全文
![点击此处可从《Plant biotechnology journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Thuanne Pires Ribeiro Fabricio Barbosa Monteiro Arraes Isabela Tristan Lourenço‐Tessutti Marilia Santos Silva Maria Eugênia Lisei‐de‐Sá Wagner Alexandre Lucena Leonardo Lima Pepino Macedo Janaina Nascimento Lima Regina Maria Santos Amorim Sinara Artico Márcio Alves‐Ferreira Maria Cristina Mattar Silva Maria Fatima Grossi‐de‐Sa 《Plant biotechnology journal》2017,15(8):997-1009
993.
Young-Lim Lee Haruko Takeda Gabriel Costa Monteiro Moreira Latifa Karim Erik Mullaart Wouter Coppieters The GplusE consortium Ruth Appeltant Roel F. Veerkamp Martien A. M. Groenen Michel Georges Mirte Bosse Tom Druet Aniek C. Bouwman Carole Charlier 《PLoS genetics》2021,17(7)
Clinical mastitis (CM) is an inflammatory disease occurring in the mammary glands of lactating cows. CM is under genetic control, and a prominent CM resistance QTL located on chromosome 6 was reported in various dairy cattle breeds. Nevertheless, the biological mechanism underpinning this QTL has been lacking. Herein, we mapped, fine-mapped, and discovered the putative causal variant underlying this CM resistance QTL in the Dutch dairy cattle population. We identified a ~12 kb multi-allelic copy number variant (CNV), that is in perfect linkage disequilibrium with a lead SNP, as a promising candidate variant. By implementing a fine-mapping and through expression QTL mapping, we showed that the group-specific component gene (GC), a gene encoding a vitamin D binding protein, is an excellent candidate causal gene for the QTL. The multiplicated alleles are associated with increased GC expression and low CM resistance. Ample evidence from functional genomics data supports the presence of an enhancer within this CNV, which would exert cis-regulatory effect on GC. We observed that strong positive selection swept the region near the CNV, and haplotypes associated with the multiplicated allele were strongly selected for. Moreover, the multiplicated allele showed pleiotropic effects for increased milk yield and reduced fertility, hinting that a shared underlying biology for these effects may revolve around the vitamin D pathway. These findings together suggest a putative causal variant of a CM resistance QTL, where a cis-regulatory element located within a CNV can alter gene expression and affect multiple economically important traits. 相似文献
994.
Weerayuth Supiwong Thomas Liehr Marcelo B. Cioffi Arunrat Chaveerach Nadezda Kosyakova Krit Pinthong Tawatchai Tanee Alongklod Tanomtong 《Zoologischer Anzeiger》2014
We analyzed the distribution of repetitive DNA sequences on the chromosomes of nine species of the Bagridae from Thailand, i.e., Hemibagrus filamentus; H. nemurus; H. wyckii; H. wyckioides; Mystus atrifasciatus; M. multiradiatus; M. mysticetus; M. bocourti and Pseudomystus siamensis. Two classes of microsatellites and one transposable element (TE) were mapped by fluorescence in situ hybridization. The distribution of the repetitive sequences was comparatively analyzed in view to investigate their contribution in the chromosomal evolution of this fish group. In all species the microsatellites (CA)15 and (GA)15 are abundantly distributed in all chromosomes, usually in the telomeric regions. The retrotransposable element Rex 1 is widely distributed over the whole genome including heterochromatin and euchromatin, but with an unexpected accumulation in one chromosome pair in some species. In fact, some species–specific patterns could be observed considering both microsatellites and TE distribution. The results demonstrated that the compartmentalization of repeated elements is not simply restricted to heterochromatic regions, as it has been postulated in the first concepts of the genomic organization of repetitive elements in genomes. Moreover, the organization of these repeats seems to reflect their intense and specific evolutionary pathway, providing new insights about the chromosomal evolution in the Bagridae. 相似文献
995.
996.
Tatiane A. Paixão Marcelo C. C. Malta Semíramis A. Soave Herlandes P. Tinoco Maria E.L.T. Costa Angela T. Pessanha Rodrigo O.S. Silva Fernanda M. Coura Luciana F. Costa Andreia P. Turchetti Francisco C.F. Lobato Marilia M. Melo Marcos B. Heinemann Renato L. Santos 《Journal of medical primatology》2014,43(2):118-121
997.
Laura Azeredo Miranda Mota Jo?o Roberto Neto Ver?nica Gomes Monteiro Caroliny Samary Silva Lobato Marco Antonio de Oliveira Maura da Cunha Heloisa D’ávila Sérgio Henrique Seabra Patrícia Torres Bozza Renato Augusto DaMatta 《Memórias do Instituto Oswaldo Cruz》2014,109(6):767-774
Lipid bodies [lipid droplets (LBs)] are lipid-rich organelles involved in lipid
metabolism, signalling and inflammation. Recent findings suggest a role for LBs in
host response to infection; however, the potential functions of this organelle
in Toxoplasma gondii infection and how it alters macrophage
microbicidal capacity during infection are not well understood. Here, we investigated
the role of host LBs in T. gondii infection in mouse peritoneal
macrophages in vitro. Macrophages cultured with mouse serum (MS) had higher numbers
of LBs than those cultured in foetal bovine serum and can function as a model to
study the role of LBs during intracellular pathogen infection. LBs were found in
association with the parasitophorous vacuole, suggesting that T. gondii
may benefit from this lipid source. Moreover, increased numbers of
macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased
nitric oxide (NO) synthesis. Accordingly, LB-enriched macrophages cultured with MS
were less efficient at controlling T. gondii growth. Treatment of
macrophages cultured with MS with indomethacin, an inhibitor of PGE2 production,
increased the microbicidal capacity against T. gondii. Collectively,
these results suggest that culture with MS caused a decrease in microbicidal activity
of macrophages against T. gondii by increasing PGE2 while lowering
NO production. 相似文献
998.
Eduardo Massad Annelies Wilder-Smith Raphael Ximenes Marcos Amaku Luis Fernandez Lopez Francisco Antonio Bezerra Coutinho Giovanini Evelim Coelho Jarbas Barbosa da Silva Jr Claudio José Struchiner Marcelo Nascimento Burattini 《Memórias do Instituto Oswaldo Cruz》2014,109(3):394-397
Brazil will host the FIFA World Cup™, the biggest single-event competition in the
world, from June 12-July 13 2014 in 12 cities. This event will draw an estimated
600,000 international visitors. Brazil is endemic for dengue. Hence, attendees of the
2014 event are theoretically at risk for dengue. We calculated the risk of dengue
acquisition to non-immune international travellers to Brazil, depending on the
football match schedules, considering locations and dates of such matches for June
and July 2014. We estimated the average per-capita risk and expected
number of dengue cases for each host-city and each game schedule chosen based on
reported dengue cases to the Brazilian Ministry of Health for the period between
2010-2013. On the average, the expected number of cases among the 600,000 foreigner
tourists during the World Cup is 33, varying from 3-59. Such risk estimates will not
only benefit individual travellers for adequate pre-travel preparations, but also
provide valuable information for public health professionals and policy makers
worldwide. Furthermore, estimates of dengue cases in international travellers during
the World Cup can help to anticipate the theoretical risk for exportation of dengue
into currently non-infected areas. 相似文献
999.
Fernanda Aparecida Nieri-Bastos Marcos Gomes Lopes Paulo Henrique Duarte Can?ado Giselle Ayres Razera Rossa Jo?o Luiz Horácio Faccini Solange Maria Gennari Marcelo Bahia Labruna 《Memórias do Instituto Oswaldo Cruz》2014,109(2):259-261
Adult ticks of the species Amblyomma parvum were collected from the
vegetation in the Pantanal biome (state of Mato Grosso do Sul) and from horses in the
Cerrado biome (state of Piauí) in Brazil. The ticks were
individually tested for rickettsial infection via polymerase chain reaction (PCR)
targeting three rickettsial genes, gltA, ompA and
ompB. Overall, 63.5% (40/63) and 66.7% (2/3) of A.
parvum ticks from Pantanal and Cerrado, respectively, contained
rickettsial DNA, which were all confirmed by DNA sequencing to be 100% identical to
the corresponding fragments of the gltA, ompA and
ompB genes of Candidatus Rickettsia andeanae.
This report is the first to describe Ca. R. andeanae in Brazil. 相似文献
1000.
Maricela Robles-Murguia Nicholas Bloedow Leigh Murray Marcelo Ramalho-Ortig?o 《Memórias do Instituto Oswaldo Cruz》2014,109(8):1064-1069
In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood
digestion. Also, the kinetics of PM secretion varies according to species. We
previously characterised PpChit1, a midgut-specific chitinase secreted in
Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM
and showed that antibodies against PpChit1 reduce the chitinolytic activity in the
midgut of several sandfly species. Here, sandflies were fed on red blood cells
reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters
that included blood digestion, oviposition onset, number of eggs laid, egg bouts,
average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day
delay in the onset of egg laying, with flies surviving three days longer compared to
the control group. Anti-PpChit1 also had a negative effect on overall ability of
flies to lay eggs, as several gravid females from all three species were unable to
lay any eggs despite having lived longer than control flies. Whereas the longer
survival might be associated with improved haeme scavenging ability by the PM, the
inability of females to lay eggs is possibly linked to changes in PM permeability
affecting nutrient absorption. 相似文献