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991.
This is the first record of Acanthoscelides schrankiae Horn. feeding in seeds of Mimosa bimucronata (DC.) Kuntze. We investigated the pattern of oviposition and seed exploitation by A. schrankiae, and the distribution of mature fruits and seed predation in the inflorescences. We also compared the percentage of predated seeds, the total dry weight of fruits and non-predated seeds, the percentage of aborted seeds, and the percentage of non-emergent insects, among different quadrants of the M. bimucronata canopy. To determine the occurring species, the emergence of bruchids and parasitoids was observed in the laboratory, resulting altogether, only in individuals of A. schrankiae and Horismenus sp. (Hymenoptera: Eulophidae) species, respectively. Mean number of fruits produced in the median region of inflorescence was significantly higher than in the inferior and superior regions, and the frequencies (observed and expected) of predated and non-predated seeds differed among the different regions of inflorescence. Females of A. schrankiae laid their eggs on fruits, and larvae, after emergence, perforated the exocarp to reach the seeds. Most fruits presented one to three eggs and only one bruchid larva was observed in each seed. The highest value of the rate "number of eggs/fruit" and the highest percentage of predated seeds were recorded in April. Dry weight of fruits (total) and seeds (non-predated), proportions of predated seeds, seed abortions, and non-emergent seed predators, were evenly distributed in the canopy. 相似文献
992.
MosquiTRAP is a sticky trap specifically designed to capture gravid females of Aedes aegypti (L.) and allows the identification of the mosquito in the field during the inspection of the trap. This study aims to compare this sticky trap to larval and ovitrap surveys for field monitoring of A. aegypti during the dry season. The study was conducted from March to June of 2003 in 20 blocks of the district of Itapo?, Belo Horizonte, MG. The traps were monitored every week while the larval survey was conducted on a monthly basis. The larval index: Premise Index (PI) and Breteau Index (BI) had equal values throughout the experiment (1.72 in the first two months and zero in the last two). The container index (CI) during the first two months was 0.09 and 0.1%, respectively and zero in the last two. The Ovitrap Positive Index (OPI) ranged from 16.7 to 76.9%, and the MosquiTRAP Positive Index (MPI) ranged from 0 to 31.5%. The Egg Density Index (EDI) ranged from 26.6 to 82.8, while the Adult Density Index ranged from 0 to 1.6 throughout the experiment. Temperature and rainfall did not affect the Positive and Density Indices, although these environmental variables seemed to have affected the larvae indices. Although the MosquiTRAP caught a low number of Aedes mosquitoes during the study, it was more sensitive than the larval survey to detect the presence of Aedes mosquitoes. 相似文献
993.
Barros NM Campos M Bersanetti PA Oliveira V Juliano MA Boileau G Juliano L Carmona AK 《Biological chemistry》2007,388(4):447-455
We examined the substrate specificity of the carboxydipeptidase activity of neprilysin (NEP) using fluorescence resonance energy transfer (FRET) peptides containing ortho-aminobenzoyl (Abz) and 2,4-dinitrophenyl (Dnp) as a donor/acceptor pair. Two peptide series with general sequences Abz-RXFK(Dnp)-OH and Abz-XRFK(Dnp)-OH (X denotes the position of the altered amino acid) were synthesized to study P1 (cleavage at the X-F bond) and P2 (cleavage at R-F bond) specificity, respectively. In these peptides a Phe residue was fixed in P1' to fulfill the well-known NEP S1' site requirement for a hydrophobic amino acid. In addition, we explored NEP capability to hydrolyze bradykinin (RPPGFSPFR) and its fluorescent derivative Abz-RPPGFSPFRQ-EDDnp (EDDnp=2,4-dinitrophenyl ethylenediamine). The enzyme acts upon bradykinin mainly as a carboxydipeptidase, preferentially cleaving Pro-Phe over the Gly-Phe bond in a 9:1 ratio, whereas Abz-RPPGFSPFRQ-EDDnp was hydrolyzed at the same bonds but at an inverted proportion of 1:9. The results show very efficient interaction of the substrates' C-terminal free carboxyl group with site S2' of NEP, confirming the enzyme's preference to act as carboxydipeptidase at substrates with a free carboxyl-terminus. Using data gathered from our study, we developed sensitive and selective NEP substrates that permit continuous measurement of the enzyme activity, even in crude tissue extracts. 相似文献
994.
Spinelli Oliveira E Hancock JT Hermes-Lima M Isola DA Ochs M Yu J Wilhem Filho D 《Integrative and comparative biology》2007,47(4):578-591
A gas-exchange structure interacts with the environment andis constantly challenged by contaminants that may elicit defenseresponses, thus compromising its primary function. It is alsoexposed to high concentrations of O2 that can generate reactiveoxygen species (ROS). Revisiting the lung of mammals, an integrativepicture emerges, indicating that this bronchi-alveolar structuredeals with inflammation in a special way, which minimizes compromisingthe gas-exchange role. Depending on the challenge, pro-inflammatoryor antiinflammatory responses are elicited by conserved molecules,such as surfactant proteins A and D. An even broader picturepoints to the participation of airway sensors, responsive toinflammatory mediators, in a loop linking the immunologicaland nervous systems and expanding the role played by respiratoryorgans in functions other than gas-exchange. A byproduct ofexposure to high concentration of O2 is the formation of superoxide(), hydrogen peroxide (H2O2),hydroxyl radical (HO), and other ROS, which are knownto be toxic to different types of cells, including the lungepithelium. A balance between antioxidants and oxidants exists;in pulmonary epithelial cells high intracellular and extracellularlevels of antioxidants are found. Antioxidant adaptations relatedto plant and animal life-styles involve a broad range of overlappingstrategies based on well-conserved molecules. Glutathione (GSH)is an abundant and ubiquitous thiol-tripeptide antioxidant,also present in lungs, whose role in providing information onthe intracellular redox state of animals and plants is wellestablished. In these organisms, GSH influences gene expressionassociated with stress, maximizing defense responses. Severalenzymatic antioxidants, such as glutathione peroxidase (GPx),glutathione reductase, glutathione S-transferase, and glucose6-phosphate dehydrogenase participate in the redox system; inanimals that are stress-tolerant GPx is a key element againstoxidative assaults. Most importantly, alternative roles of ROSas signaling molecules have been found in all plants and animals.For example, alveolar macrophages produce that act as second messengers, in addition tohaving a bactericidal role. The nonradical ROS H2O2 signalsinflammation in mammalian lungs, apoptosis in different animaltissues, and is also involved in stomatal closure, root development,gene expression, and defense responses of plants. Antioxidantadaptations in some water-breathing animals involve the excretionof H2O2 by diffusion through gas-exchange structures. The finebalance among a multitude of factors and cells makes the differencebetween damage and protection in animals and plants. Knowledgeabout the mechanisms and consequences of these molecular interactionsis now starting to be integrated. 相似文献
995.
Jessica D. Wiley Emilio F. Merino Priscilla M. Krai Kyle J. McLean Abhai K. Tripathi Joel Vega-Rodríguez Marcelo Jacobs-Lorena Michael Klemba Maria B. Cassera 《Eukaryotic cell》2015,14(2):128-139
The malaria parasite harbors a relict plastid called the apicoplast and its discovery opened a new avenue for drug discovery and development due to its unusual, nonmammalian metabolism. The apicoplast is essential during the asexual intraerythrocytic and hepatic stages of the parasite, and there is strong evidence supporting its essential metabolic role during the mosquito stages of the parasite. Supply of the isoprenoid building blocks isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) is the essential metabolic function of the apicoplast during the asexual intraerythrocytic stages. However, the metabolic role of the apicoplast during gametocyte development, the malaria stages transmitted to the mosquito, remains unknown. In this study, we showed that production of IPP for isoprenoid biosynthesis is the essential metabolic function of the apicoplast during gametocytogenesis, by obtaining normal gametocytes lacking the apicoplast when supplemented with IPP. When IPP supplementation was removed early in gametocytogenesis, developmental defects were observed, supporting the essential role of isoprenoids for normal gametocytogenesis. Furthermore, mosquitoes infected with gametocytes lacking the apicoplast developed fewer and smaller oocysts that failed to produce sporozoites. This finding further supports the essential role of the apicoplast in establishing a successful infection in the mosquito vector. Our study supports isoprenoid biosynthesis as a valid drug target for development of malaria transmission-blocking inhibitors. 相似文献
996.
997.
998.
Dantas-Torres F Aléssio FM Siqueira DB Mauffrey JF Marvulo MF Martins TF Moraes-Filho J Camargo MC D'Auria SR Labruna MB Silva JC 《Parasitology》2012,139(1):83-91
Between December 2007 and March 2009, small mammals were captured in 6 Atlantic Forest patches in Brazil. We assessed tick-host associations and whether they differ among forest strata, sites, seasons, and host age classes or between sexes. Moreover, we assessed the exposure of animals to Rickettsia spp. In total, 432 animals were captured and 808 ticks were found on 32·9% of them. Significant differences were found among host species, collection sites, and forest strata; microhabitat preference was a strong risk factor for tick infestation. The highest tick density rates were recorded in forest fragments settled in rural areas; 91·3% of the ticks were collected from animals trapped in these forest fragments. A high prevalence (68·8%) of antibodies to Rickettsia spp. was detected among animals. This study suggests that disturbed Atlantic Forest fragments provide an environment for ticks and small mammals, which are highly exposed to rickettsiae. It also indicates that forest patches settled in rural areas are usually associated with higher small mammal diversity as well as with higher tick density rates. 相似文献
999.
de Souza IV Gondim MG Ramos AL Dos Santos EA Ferraz MI Oliveira AR 《Experimental & applied acarology》2012,58(3):221-233
Aceria guerreronis Keifer is a major coconut pest in the Americas, Africa and some Asian countries, and occurs in high population levels in northeastern Brazil. The determination of the climatic conditions that favorably affect its population growth and the prevalence and abundance of predatory mites can promote more efficient control practices. Our objective was to evaluate the pattern of occurrence and population dynamics of A. guerreronis, their associated predators and other mites during a 2?year period in a hybrid coconut plantation in the municipality of Una, state of Bahia, northeastern Brazil. Monthly samples of fruits were taken from June 2008 to May 2010 for qualitative and quantitative evaluation of mites. Aceria guerreronis represented 99.9?% of the mites. An average density of 1,117 mites per fruit and a maximum of 23,596 mites per fruit indicated that the level of infestation can be high in Bahia. Bdella ueckermanni Hernandes, Daud and Feres was the most abundant and frequent predator. Population increase of A. guerreronis was directly related to the temperature rise and inversely related to both the increase of air relative humidity and rainfall. The highest population densities occurred from November to March. The largest A. guerreronis populations occurred in fruits with 32 and 48?% of damaged surface. The relationship between prevailing wind direction and incidence of A. guerreronis could not be corroborated or refuted. 相似文献
1000.
Rac signaling in breast cancer: a tale of GEFs and GAPs 总被引:1,自引:0,他引:1
Wertheimer E Gutierrez-Uzquiza A Rosemblit C Lopez-Haber C Sosa MS Kazanietz MG 《Cellular signalling》2012,24(2):353-362
Rac GTPases, small G-proteins widely implicated in tumorigenesis and metastasis, transduce signals from tyrosine-kinase, G-protein-coupled receptors (GPCRs), and integrins, and control a number of essential cellular functions including motility, adhesion, and proliferation. Deregulation of Rac signaling in cancer is generally a consequence of enhanced upstream inputs from tyrosine-kinase receptors, PI3K or Guanine nucleotide Exchange Factors (GEFs), or reduced Rac inactivation by GTPase Activating Proteins (GAPs). In breast cancer cells Rac1 is a downstream effector of ErbB receptors and mediates migratory responses by ErbB1/EGFR ligands such as EGF or TGFα and ErbB3 ligands such as heregulins. Recent advances in the field led to the identification of the Rac-GEF P-Rex1 as an essential mediator of Rac1 responses in breast cancer cells. P-Rex1 is activated by the PI3K product PIP3 and Gβγ subunits, and integrates signals from ErbB receptors and GPCRs. Most notably, P-Rex1 is highly overexpressed in human luminal breast tumors, particularly those expressing ErbB2 and estrogen receptor (ER). The P-Rex1/Rac signaling pathway may represent an attractive target for breast cancer therapy. 相似文献