全文获取类型
收费全文 | 4081篇 |
免费 | 238篇 |
国内免费 | 2篇 |
专业分类
4321篇 |
出版年
2024年 | 2篇 |
2023年 | 37篇 |
2022年 | 58篇 |
2021年 | 117篇 |
2020年 | 99篇 |
2019年 | 107篇 |
2018年 | 102篇 |
2017年 | 112篇 |
2016年 | 202篇 |
2015年 | 233篇 |
2014年 | 231篇 |
2013年 | 314篇 |
2012年 | 363篇 |
2011年 | 335篇 |
2010年 | 239篇 |
2009年 | 174篇 |
2008年 | 254篇 |
2007年 | 256篇 |
2006年 | 216篇 |
2005年 | 192篇 |
2004年 | 147篇 |
2003年 | 154篇 |
2002年 | 132篇 |
2001年 | 23篇 |
2000年 | 28篇 |
1999年 | 25篇 |
1998年 | 34篇 |
1997年 | 21篇 |
1996年 | 20篇 |
1995年 | 15篇 |
1994年 | 13篇 |
1993年 | 9篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 11篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 6篇 |
1982年 | 1篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1975年 | 1篇 |
排序方式: 共有4321条查询结果,搜索用时 14 毫秒
101.
María Cecilia De Rossi Luciana Bruno Alejandro Wolosiuk Marcelo A Despósito Valeria Levi 《Biochimica et Biophysica Acta (BBA)/General Subjects》2013
Background
Organelle transport is driven by the action of molecular motors. In this work, we studied the dynamics of organelles of different sizes with the aim of understanding the complex relation between organelle motion and microenvironment.Methods
We used single particle tracking to obtain trajectories of melanosomes (pigmented organelles in Xenopus laevis melanophores). In response to certain hormones, melanosomes disperse in the cytoplasm or aggregate in the perinuclear region by the combined action of microtubule and actin motors.Results and conclusions
Melanosome trajectories followed an anomalous diffusion model in which the anomalous diffusion exponent (α) provided information regarding the trajectories' topography and thus of the processes causing it. During aggregation, the directionality of big organelles was higher than that of small organelles and did not depend on the presence of either actin or intermediate filaments (IF). Depolymerization of IF significantly reduced α values of small organelles during aggregation but slightly affect their directionality during dispersion.General significance
Our results could be interpreted considering that the number of copies of active motors increases with organelle size. Transport of big organelles was not influenced by actin or IF during aggregation showing that these organelles are moved processively by the collective action of dynein motors. Also, we found that intermediate filaments enhance the directionality of small organelles suggesting that this network keeps organelles close to the tracks allowing their efficient reattachment. The higher directionality of small organelles during dispersion could be explained considering the better performance of kinesin-2 vs. dynein at the single molecule level. 相似文献102.
Vanessa A. Belo Marcelo R. Luizon Patrícia C. Carneiro Valéria A. Gomes Riccardo Lacchini Carla M. M. Lanna Debora C. Souza-Costa Jose E. Tanus-Santos 《Molecular biology reports》2013,40(3):2697-2704
Matrix metalloproteinase-2 is involved in the development of the adipose tissue, and associated with cardiovascular diseases. Metabolic risk factors (MRFs) and functional polymorphisms in the MMP-2 gene may affect its expression and activity. We investigated whether traditional MRFs and two MMP-2 gene polymorphisms (C?1306T; rs243865, and C?735T; rs2285053) affect circulating MMP-2 levels in children and adolescents, and whether MMP-2 polymorphisms and/or haplotype are associated with susceptibility to childhood obesity. We studied 114 healthy controls, 43 obese, and 83 obese with ≥3 MRFs children and adolescents. Genotypes were determined by Taqman allele discrimination assay and real-time PCR. Plasma MMP-2 was measured using zymography. We found positive correlations between MMP-2 concentrations and mean blood pressure in all children and adolescents group (r = 0.132; P < 0.05) and in obese children and adolescents (r = 0.247; P < 0.01). We found that the CC genotype for the C?1306T polymorphism was more common in subjects with higher MMP-2 concentrations in controls (P = 0.003) and in the obese group (P = 0.013). The CT genotype (OR = 0.40; P < 0.01) and the T allele (OR = 0.48; P < 0.01) for the C?735T polymorphism were less common in obese children and adolescents than in controls. The haplotypes distribution did not show significant differences between control and obese (P > 0.05). Ours findings show that blood pressure is associated with circulating MMP-2 concentrations, and that the CC genotype for the C?1306T polymorphism was more common subjects (controls and obese) with higher MMP-2 concentrations, whereas the CT genotype and the T allele for the C?735T polymorphism are less common in obesity. 相似文献
103.
Andrea Engelbrecht Thomas Mörs Marcelo A. Reguero Jürgen Kriwet 《Historical Biology》2013,25(8):1028-1044
AbstractEocene deposits of the famous La Meseta Formation of Seymour Island, Antarctic Peninsula, yielded the most diverse Paleogene fossil elasmobranch association of the Southern Hemisphere. In this assemblage, sharks clearly dominate the fauna, whereas batoids are very rare components. Herein, we describe two new taxa of cold water tolerant skates, Marambioraja leiostemma gen. et sp. nov., and Mesetaraja maleficapelli gen. et sp. nov., two new species of the genus Raja, Raja amphitrita sp. nov. and Raja manitaria sp. nov., as well as remains of warm water adapted myliobatiforms. It is, however, not possible to unambiguously assign these remains either to Myliobatidae or Rhinopteridae, or to any specific genus. Previously reported remains of Raja/Bathyraja sp. are assigned to the new described species Raja manitaria sp. nov. The biogeographic distribution of extant and extinct rays and skates clearly shows that both groups are more widely distributed today than in the past, and additionally seem to have been more diverse in the Northern than the Southern Hemisphere. The occurrence, albeit rare of isolated teeth of skates (Rajidae) and rays (Myliobatidae) in the La Meseta Formation representes a minimum age constraint for their first appearance in the Southern Ocean. http://www.zoobank.org/urn:lsid:zoobank.org:pub:E507D86C-FFEC-4047-A337-AE77606BB1A6 相似文献
104.
João Gabriel Silva Souza Carolina Veloso Lima Bárbara Emanoele Costa Oliveira Antônio Pedro Ricomini-Filho Marcelo Faveri Cortino Sukotjo 《Biofouling》2013,29(10):1175-1184
AbstractThis study aimed to test the dose-response effect of chlorhexidine on multispecies biofilms formed on commercially pure titanium (cpTi) and titanium-zirconium (TiZr) alloy. Biofilms were formed on cpTi and TiZr discs and treated two times per day with five different chlorhexidine concentrations (0.12, 0.20, 0.50, 1, 2%). The biofilms were collected for microbiological, biochemical and microscopic analyses. The significance of differences among groups was evaluated by linear regression, ANOVA, Bonferroni and Tukey tests. The mean number of colony-forming units decreased as the chlorhexidine concentration increased for both cpTi and TiZr (p?<?0.05). The maximum effect was observed with the 0.5% concentration. Confocal microscopy images suggested an increase in the number of dead bacterial cells with increased chlorhexidine concentration. The biofilm pH increased after chlorhexidine exposure (p?<?0.05). Chlorhexidine showed an antimicrobial dose-response effect in controlling biofilm on cpTi and TiZr. 0.5% chlorhexidine can be used to achieve the maximum antimicrobial effect on both materials. 相似文献
105.
106.
Marcelo Aranda Víctor Sánchez-Cordero 《Studies on Neotropical Fauna and Environment》2013,48(2):65-67
At the Calakmul Biosphere Reserve in Campeche, Mexico, the prey spectra of sympatric jaguars (Panthera onca) and pumas (Puma concolor) were studied by examination of their scats. 10 vertebrate species were identified for jaguars and 7 for pumas, mainly mammals and some birds. Based on these diet analyses, we conclude that jaguars and pumas coexist at Calakmul by means of different food habits. 相似文献
107.
Juliana L. França Marcelo R. Pinto Malson N. Lucena Daniela P. Garçon Wagner C. Valenti John C. McNamara Francisco A. Leone 《The Journal of membrane biology》2013,246(7):529-543
The stimulation by Mg2+, Na+, K+, NH4 +, and ATP of (Na+, K+)-ATPase activity in a gill microsomal fraction from the freshwater prawn Macrobrachium rosenbergii was examined. Immunofluorescence labeling revealed that the (Na+, K+)-ATPase α-subunit is distributed predominantly within the intralamellar septum, while Western blotting revealed a single α-subunit isoform of about 108 kDa M r. Under saturating Mg2+, Na+, and K+ concentrations, the enzyme hydrolyzed ATP, obeying cooperative kinetics with V M = 115.0 ± 2.3 U mg?1, K 0.5 = 0.10 ± 0.01 mmol L?1. Stimulation by Na+ (V M = 110.0 ± 3.3 U mg?1, K 0.5 = 1.30 ± 0.03 mmol L?1), Mg2+ (V M = 115.0 ± 4.6 U mg?1, K 0.5 = 0.96 ± 0.03 mmol L?1), NH4 + (V M = 141.0 ± 5.6 U mg?1, K 0.5 = 1.90 ± 0.04 mmol L?1), and K+ (V M = 120.0 ± 2.4 U mg?1, K M = 2.74 ± 0.08 mmol L?1) followed single saturation curves and, except for K+, exhibited site–site interaction kinetics. Ouabain inhibited ATPase activity by around 73 % with K I = 12.4 ± 1.3 mol L?1. Complementary inhibition studies suggest the presence of F0F1–, Na+-, or K+-ATPases, but not V(H+)- or Ca2+-ATPases, in the gill microsomal preparation. K+ and NH4 + synergistically stimulated enzyme activity (≈25 %), suggesting that these ions bind to different sites on the molecule. We propose a mechanism for the stimulation by both NH4 +, and K+ of the gill enzyme. 相似文献
108.
109.
Kazima Saira Xudong Lin Jay V. DePasse Rebecca Halpin Alan Twaddle Timothy Stockwell Brian Angus Alessandro Cozzi-Lepri Marina Delfino Vivien Dugan Dominic E. Dwyer Matthew Freiberg Andrzej Horban Marcelo Losso Ruth Lynfield Deborah N. Wentworth Edward C. Holmes Richard Davey David E. Wentworth Elodie Ghedin 《Journal of virology》2013,87(14):8064-8074
Influenza virus defective interfering (DI) particles are naturally occurring noninfectious virions typically generated during in vitro serial passages in cell culture of the virus at a high multiplicity of infection. DI particles are recognized for the role they play in inhibiting viral replication and for the impact they have on the production of infectious virions. To date, influenza virus DI particles have been reported primarily as a phenomenon of cell culture and in experimentally infected embryonated chicken eggs. They have also been isolated from a respiratory infection of chickens. Using a sequencing approach, we characterize several subgenomic viral RNAs from human nasopharyngeal specimens infected with the influenza A(H1N1)pdm09 virus. The distribution of these in vivo-derived DI-like RNAs was similar to that of in vitro DIs, with the majority of the defective RNAs generated from the PB2 (segment 1) of the polymerase complex, followed by PB1 and PA. The lengths of the in vivo-derived DI-like segments also are similar to those of known in vitro DIs, and the in vivo-derived DI-like segments share internal deletions of the same segments. The presence of identical DI-like RNAs in patients linked by direct contact is compatible with transmission between them. The functional role of DI-like RNAs in natural infections remains to be established. 相似文献
110.
Marcelo Manzi Marinho Maria Betânia Gonçalves Souza Miquel Lürling 《Microbial ecology》2013,66(3):479-488
The hypothesis that outcomes of phosphorus and light competition between Cylindrospermopsis raciborskii and Microcystis aeruginosa are strain dependent was tested experimentally. Critical requirements of phosphorus (P*) and of light (I*) of two strains of each species were determined through monoculture experiments, which indicated a trade-off between species and also between Microcystis strains. Competition experiments between species were performed using the weakest predicted competitors (with the highest values of P* and of I*) and with the strongest predicted competitors (with the lowest values of P* and of I*). Under light limitation, competition between the weakest competitors led C. raciborskii to dominate. Between the strongest competitors, the opposite was observed, M. aeruginosa displaced C. raciborskii, but both strains co-existed in equilibrium. Under phosphate limitation, competition between the weakest competitors led C. raciborskii to exclude M. aeruginosa, and between the strongest competitors, the opposite was observed, M. aeruginosa displaced C. raciborskii, but the system did not reach an equilibrium and both strains were washed out. Hence, outcomes of the competition depended on the pair of competing strains and not only on species or on type of limitation. We concluded that existence of different trade-offs among strains and between species underlie our results showing that C. raciborskii can either dominate or be displaced by M. aeruginosa when exposed to different conditions of light or phosphate limitation. 相似文献