全文获取类型
收费全文 | 3976篇 |
免费 | 356篇 |
国内免费 | 2篇 |
专业分类
4334篇 |
出版年
2023年 | 26篇 |
2022年 | 40篇 |
2021年 | 61篇 |
2020年 | 53篇 |
2019年 | 60篇 |
2018年 | 65篇 |
2017年 | 84篇 |
2016年 | 123篇 |
2015年 | 178篇 |
2014年 | 223篇 |
2013年 | 255篇 |
2012年 | 333篇 |
2011年 | 304篇 |
2010年 | 218篇 |
2009年 | 170篇 |
2008年 | 232篇 |
2007年 | 249篇 |
2006年 | 204篇 |
2005年 | 199篇 |
2004年 | 181篇 |
2003年 | 156篇 |
2002年 | 179篇 |
2001年 | 50篇 |
2000年 | 50篇 |
1999年 | 52篇 |
1998年 | 47篇 |
1997年 | 31篇 |
1996年 | 45篇 |
1995年 | 32篇 |
1994年 | 31篇 |
1993年 | 22篇 |
1992年 | 20篇 |
1991年 | 28篇 |
1990年 | 27篇 |
1989年 | 32篇 |
1988年 | 33篇 |
1987年 | 15篇 |
1986年 | 16篇 |
1985年 | 19篇 |
1984年 | 16篇 |
1983年 | 13篇 |
1982年 | 12篇 |
1981年 | 13篇 |
1980年 | 12篇 |
1979年 | 14篇 |
1978年 | 8篇 |
1977年 | 9篇 |
1976年 | 11篇 |
1974年 | 7篇 |
1973年 | 14篇 |
排序方式: 共有4334条查询结果,搜索用时 0 毫秒
51.
Swamp gorillas in northern Congo 总被引:3,自引:0,他引:3
52.
Abousalham Abdelkarim; Teissere Marcel; Gardies Anne-Marie; Verger Robert; Noat Georges 《Plant & cell physiology》1995,36(6):989-996
Phospholipase D (phosphatidylcholine phosphatidohydrolase EC3.1.4.4
[EC]
) from soybean (Glycine max L.) suspension-cultured cellwas purified around 1,200-fold to homogeneity by acetone precipitation,Macro-Prep High Q anion exchange, and octyl-Sepharose CL-4Baffinity chromatography. The purified enzyme released 1,600µmol of choline per min per mg of protein. The enzymeis monomeric with a molecular mass of 92 kDa, as estimated bySDS-PAGE. One of the most interesting characteristics of thepurified soybean phospholipase D was the dependence of the pHoptimum on the Ca2+ ion concentration in the assay. With 10mM, 20 mM and 40 mM Ca2+ ions, the optima were at pH 7.5, 6and 5.5, respectively. The specific adsorption of phospholipaseD onto octyl-Sepharose gel suggests that the molecule becomesmore hydrophobic in the presence of Ca2+ ions. The amino acidsequence of the first 18 N-terminal residues of soybean phospholipaseD revealed a high degree of homology with those previously publishedfor cabbage leaf and castor bean endosperm enzymes. Westernblots of the soybean phospholipase D showed an immunoreactivitywith antibodies raised against a synthetic peptide correspondingto the 15 N-terminal aminoacid residues of phospholipase D fromcabbage leaves. (Received March 13, 1995; Accepted May 29, 1995) 相似文献
53.
54.
55.
Marcel M. Verbeek Robert M. W. de Waal Janine J. Schipper †William E. Van Nostrand 《Journal of neurochemistry》1997,68(3):1135-1141
Abstract: Amyloid β protein (Aβ) deposition in the cerebral arterial and capillary walls is one of the major characteristics of brains from patients with Alzheimer's disease and hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D). Vascular Aβ deposition is accompanied by degeneration of smooth muscle cells and pericytes. In this study we found that Aβ1–40 carrying the "Dutch" mutation (HCHWA-D Aβ1–40 ) as well as wild-type Aβ1–42 induced degeneration of cultured human brain pericytes and human leptomeningeal smooth muscle cells, whereas wild-type Aβ1–40 and HCHWA-D Aβ1–42 were inactive. Cultured brain pericytes appeared to be much more vulnerable to Aβ-induced degeneration than leptomeningeal smooth muscle cells, because in brain pericyte cultures cell viability already decreased after 2 days of exposure to HCHWA-D Aβ1–40 , whereas in leptomeningeal smooth muscle cell cultures cell death was prominent only after 4–5 days. Moreover, leptomeningeal smooth muscle cell cultures were better able to recover than brain pericyte cultures after short-term treatment with HCHWA-D Aβ1–40 . Degeneration of either cell type was preceded by an increased production of cellular amyloid precursor protein. Both cell death and amyloid precursor protein production could be inhibited by the amyloid-binding dye Congo red, suggesting that fibril assembly of Aβ is crucial for initiating its destructive effects. These data imply an important role for Aβ in inducing perivascular cell pathology as observed in the cerebral vasculature of patients with Alzheimer's disease or HCHWA-D. 相似文献
56.
Daniel Duprez Dirk Voet Marc De Buyzere Benny Drieghe Bruno Vyncke Sigrid Mareels Marcel Afschrift Denis L. Clement 《European journal of applied physiology and occupational physiology》1995,71(5):459-463
The splanchnic circulation can make a major contribution to blood flow changes. However, the role of the splanchnic circulation in the reflex adjustments to the blood pressure increase during isometric exercise is not well documented. The central command and the muscle chemoreflex are the two major mechanisms involved in the blood pressure response to isometric exercise. This study aimed to examine the behaviour of the superior mesenteric artery during isometric handgrip (IHG) at 30% maximal voluntary contraction (MVC). The pulsatility index (PI) of the blood velocity waveform of the superior mesenteric artery was taken as the study parameter. A total of ten healthy subjects [mean age, 21.1 (SEM 0.3) years] performed an IHG at 30% MVC for 90 s. At 5 s prior to the end of the exercise, muscle circulation was arrested for 90 s to study the effect of the muscle chemoreflex (post exercise arterial occlusion, PEAO). The IHG at 30% MVC caused a decrease in superior mesenteric artery PI, from 4.84 (SEM 1.57) at control level to 3.90 (SEM 1.07) (P = 0.015). The PI further decreased to 3.17 (SEM 0.70) (P = 0.01) during PEAO. Our results indicated that ergoreceptors may be involved in the superior mesenteric artery vasodilatation during isometric exercise. 相似文献
57.
58.
59.
The carbon magnetic resonance spectra of many fatty acid methyl esters with cis and trans double bonds and triple bonds at various positions and in many different combinations have been investigated.The influence of the ester group on double and triple bonds in the fatty acid chain depends strongly on the positions of these bonds. For a given position the influence is constant, even if one or more other double or triple bonds are present.Together with the evaluated chemical shift parameters for the effects of double and triple bonds on each other, complete assignments are possible and spectra of various types of unsaturated esters can be predicted with high accuracy (±0.1 ppm). 相似文献
60.
Mapping of antigenic determinants of human apolipoprotein B using monoclonal antibodies against low density lipoproteins 总被引:16,自引:0,他引:16
Y L Marcel M Hogue R Theolis R W Milne 《The Journal of biological chemistry》1982,257(22):13165-13168
The reactivity of a series of monoclonal antibodies directed against human low density lipoproteins (LDL) has been tested with hepatic and intestinal apolipoprotein B (apo-B) termed B-100 and B-48, respectively (Kane, J. P., Hardman, D. A., and Paulus, H. E. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 2465-2469). Whereas those antibodies that have been previously shown to recognize determinants close to the LDL receptor recognition site reacted only with B-100, two antibodies specific for other regions of apo-B reacted with both B-100 and B-48. Therefore, it is probable that sequence homologies exist between the two proteins and it must be considered that all or parts of the B-48 sequence may be contained within that of B-100. The specificity of the reaction of these antibodies with proteins designated B-74 and B-26 supports the concept that they represent complementary fragments of B-100. The present results have been incorporated in a theoretical map of the antigenic determinants recognized by these antibodies on the LDL apo-B. 相似文献