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991.
During periods of high energy demand an animal may be constrained by a physiological maximum to its energy intake rate. Predictions by allometric equations describing this maximum for endotherms were significantly surpassed during a few recent laboratory experiments on birds and mammals, being given access to food 24 h day?1. How relevant this is in the field remains to be assessed. We predicted that Bewick's swans Cygnus columbianus bewickii might surpass this maximum during stopover on their migration. We determined intake rate by measuring initial and final biomass density, and dividing the biomass difference by the feeding time required to reach this difference. This feeding time was given by the functional response. After conversion to daily energy intake rates, these exceeded the previously assumed maximum on two of the three stopover sites studied. The exception was a stopover site where daily foraging time was limited by the tidal cycle. Our study confirms that intake rates may exceed the formerly generally supposed maximum under natural conditions when foraging is possible day and night. 相似文献
992.
Thor Veen Ben C. Sheldon Franz J. Weissing Marcel E. Visser Anna Qvarnström Glenn-Peter Sætre 《Oecologia》2010,162(4):873-884
Many related species share the same environment and utilize similar resources. This is surprising because based on the principle of competitive exclusion one would predict that the superior competitor would drive the other species to extinction; coexistence is only predicted if interspecific competition is weaker than intraspecific competition. Interspecific competition is frequently reduced by differential resource use, resulting in habitat segregation. In this paper, we use the closely related collared and pied flycatcher to assess the potential of habitat differences to affect interspecific competition through a different mechanism, namely by generating temporal differences in availability of similar food resources between the two species. We found that the tree species composition of the breeding territories of the two species differed, mainly by a higher abundance of coniferous species around nest-boxes occupied by pied flycatchers. The temporal availability of caterpillars was measured using frass traps under four deciduous and two coniferous tree species. Deciduous tree species showed an early and narrow peak in abundance, which contrasted with the steady increase in caterpillar abundance in the coniferous tree species through the season. We subsequently calculated the predicted total caterpillar biomass available in each flycatcher territory. This differed between the species, with biomass decreasing more slowly in pied flycatcher territories. Caterpillar biomass is strongly correlated with the reproductive success of collared flycatchers, but much less so with pied flycatchers. However, caterpillar availability can only partly explain the differences in seasonal decline of reproductive success between the two species; we discuss additional factors that may contribute to this species difference. Overall, our results are consistent with the suggestion that minor habitat differences between these two species may contribute to promoting their coexistence. 相似文献
993.
The ruthenium compound [Ru2Cl(Ibp)4] (or RuIbp) has been reported to cause significantly greater inhibition of C6 glioma cell proliferation than the parent HIbp. The present study determined the effects of 0-72 h exposure to RuIbp upon C6 cell cycle distribution, mitochondrial membrane potential, reactive species generation and mRNA and protein expression of E2F1, cyclin D1, c-myc, pRb, p21, p27, p53, Ku70, Ku80, Bax, Bcl2, cyclooxygenase 1 and 2 (COX1 and COX2). The most significant changes in mRNA and protein expression were seen for the cyclin-dependent kinase inhibitors p21 and p27 which were both increased (p < 0.05). The marked decrease in mitochondrial membrane potential (p < 0.01) and modest increase in apoptosis was accompanied by a decrease in anti-apoptotic Bcl2 expression and an increase in pro-apoptotic Bax expression (p < 0.05). Interestingly, COX1 expression was increased in response to a significant loss of prostaglandin E2 production (p < 0.001), most likely due to the intracellular action of Ibp. Future studies will investigate the efficacy of this novel ruthenium-ibuprofen complex in human glioma cell lines in vitro and both rat and human glioma cells growing under orthotopic conditions in vivo. 相似文献
994.
Basic Local Alignment Search Tool, (BLAST) allows the comparison of a query sequence/s
to a database of sequences and identifies those sequences that are similar to the query above a
user-defined threshold. We have developed a user friendly web application, MULTBLAST that runs a
series of BLAST searches on a user-supplied list of proteins against one or more target protein or
nucleotide databases. The application pre-processes the data, launches each individual BLAST search
on the University of Nevada, Reno''s-TimeLogic DeCypher® system (available from
Active Motif, Inc.) and retrieves and combines all the results into a simple, easy to read output file.
The output file presents the list of the query proteins, followed by the BLAST results for the matching
sequences from each target database in consecutive columns. This format is especially useful for
either comparing the results from the different target databases, or analyzing the results while keeping
the identification of each target database separate.
Availability
The application is available at the URLhttp://blastpipe.biochem.unr.edu/ 相似文献995.
Geert Potters Nele Horemans Marcel A.K. Jansen 《Plant Physiology and Biochemistry》2010,48(5):292-300
Different redox-active compounds, such as ascorbate, glutathione, NAD(P)H and proteins from the thioredoxin superfamily, contribute to the general redox homeostasis in the plant cell. The myriad of interactions between redox-active compounds, and the effect of environmental parameters on them, has been encapsulated in the concept of a cellular redox state. This concept has facilitated progress in understanding stress signalling and defence in plants. However, despite the proven usefulness of the concept of a redox state, there is no single, operational definition that allows for quantitative analysis and hypothesis testing. 相似文献
996.
Patterns of plant invasions in China: Taxonomic,biogeographic, climatic approaches and anthropogenic effects 总被引:1,自引:0,他引:1
Shan-Huah Wu Hao-Ting Sun Yung-Ching Teng Marcel Rejmánek Shu-Miaw Chaw T.-Y. Aleck Yang Chang-Fu Hsieh 《Biological invasions》2010,12(7):2179-2206
This study was aimed to determine the patterns as well as the effects of biological, anthropogenic, and climatic factors on
plant invasions in China. About 270 volumes of national and regional floras were employed to compile a naturalized flora of
China. Habit, life form, origin, distribution, and uses of naturalized plants were also analyzed to determine patterns on
invasion. Correlations between biological, anthropogenic and climatic parameters were estimated at province and regional scales.
Naturalized species represent 1% of the flora of China. Asteraceae, Fabaceae, and Poaceae are the dominant families, but Euphorbiaceae
and Cactaceae have the largest ratios of naturalized species to their global numbers. Oenothera, Euphorbia, and Crotalaria were the dominant genera. Around 50% of exotic species were introduced intentionally for medicinal purposes. Most of the
naturalized species originated in tropical America, followed by Asia and Europe. Number of naturalized species was significantly
correlated to the number of native species/log area. The intensity of plant invasion showed a pattern along climate zones
from mesic to xeric, declining with decreasing temperature and precipitation across the nation. Anthropogenic factor, such
as distance of transportation, was significantly correlated to plant invasions at a regional scale. Although anthropogenic
factors were largely responsible for creating opportunities for exotic species to spread and establish, the local biodiversity
and climate factors were the major factors shaping the pattern of plant invasions in China. The warm regions, which are the
hot spots of local biodiversity, and relatively developed areas of China, furthermore, require immediate attentions. 相似文献
997.
Su T Bryant DM Luton F Vergés M Ulrich SM Hansen KC Datta A Eastburn DJ Burlingame AL Shokat KM Mostov KE 《Nature cell biology》2010,12(12):1143-1153
Polymeric immunoglobulin A (pIgA) transcytosis, mediated by the polymeric immunoglobulin receptor (pIgR), is a central component of mucosal immunity and a model for regulation of polarized epithelial membrane traffic. Binding of pIgA to pIgR stimulates transcytosis in a process requiring Yes, a Src family tyrosine kinase (SFK). We show that Yes directly phosphorylates EGF receptor (EGFR) on liver endosomes. Injection of pIgA into rats induced EGFR phosphorylation. Similarly, in MDCK cells, pIgA treatment significantly increased phosphorylation of EGFR on various sites, subsequently activating extracellular signal-regulated protein kinase (ERK). Furthermore, we find that the Rab11 effector Rab11-FIP5 is a substrate of ERK. Knocking down Yes or Rab11-FIP5, or inhibition of the Yes-EGFR-ERK cascade, decreased pIgA-pIgR transcytosis. Finally, we demonstrate that Rab11-FIP5 phosphorylation by ERK controls Rab11a endosome distribution and pIgA-pIgR transcytosis. Our results reveal a novel Yes-EGFR-ERK-FIP5 signalling network for regulation of pIgA-pIgR transcytosis. 相似文献
998.
Matagne Heutink Marcel WM Post Peter Luthart Lilian EMA Pfennings Catja A Dijkstra Eline Lindeman 《BMC neurology》2010,10(1):96
Background
Most people with a spinal cord injury rate neuropathic pain as one of the most difficult problems to manage and there are no medical treatments that provide satisfactory pain relief in most people. Furthermore, psychosocial factors have been considered in the maintenance and aggravation of neuropathic spinal cord injury pain. Psychological interventions to support people with spinal cord injury to deal with neuropathic pain, however, are sparse. The primary aim of the CONECSI (COping with NEuropathiC Spinal cord Injury pain) trial is to evaluate the effects of a multidisciplinary cognitive behavioural treatment programme on pain intensity and pain-related disability, and secondary on mood, participation in activities, and life satisfaction. 相似文献999.
The launch of a new thematic series of Malaria Journal -- "Towards malaria elimination" -- creates the forum that allows carrying scientific evidence on how to achieve malaria elimination in specific endemic settings and conditions into the circles of scientists, public health specialists, national and global programme managers, funders and decision makers. 相似文献
1000.
Deng W Garrett C Dombert B Soura V Banks G Fisher EM van der Brug MP Hafezparast M 《The Journal of biological chemistry》2010,285(51):39922-39934
A single amino acid change, F580Y (Legs at odd angles (Loa), Dync1h1(Loa)), in the highly conserved and overlapping homodimerization, intermediate chain, and light intermediate chain binding domain of the cytoplasmic dynein heavy chain can cause severe motor and sensory neuron loss in mice. The mechanism by which the Loa mutation impairs the neuron-specific functions of dynein is not understood. To elucidate the underlying molecular mechanisms of neurodegeneration arising from this mutation, we applied a cohort of biochemical methods combined with in vivo assays to systemically study the effects of the mutation on the assembly of dynein and its interaction with dynactin. We found that the Loa mutation in the heavy chain leads to increased affinity of this subunit of cytoplasmic dynein to light intermediate and a population of intermediate chains and a suppressed association of dynactin to dynein. These data suggest that the Loa mutation drives the assembly of cytoplasmic dynein toward a complex with lower affinity to dynactin and thus impairing transport of cargos that tether to the complex via dynactin. In addition, we detected up-regulation of kinesin light chain 1 (KLC1) and its increased association with dynein but reduced microtubule-associated KLC1 in the Loa samples. We provide a model describing how up-regulation of KLC1 and its interaction with cytoplasmic dynein in Loa could play a regulatory role in restoring the retrograde and anterograde transport in the Loa neurons. 相似文献