全文获取类型
收费全文 | 13055篇 |
免费 | 1072篇 |
国内免费 | 9篇 |
专业分类
14136篇 |
出版年
2023年 | 54篇 |
2022年 | 100篇 |
2021年 | 242篇 |
2020年 | 137篇 |
2019年 | 203篇 |
2018年 | 241篇 |
2017年 | 219篇 |
2016年 | 322篇 |
2015年 | 618篇 |
2014年 | 644篇 |
2013年 | 816篇 |
2012年 | 1072篇 |
2011年 | 1027篇 |
2010年 | 639篇 |
2009年 | 592篇 |
2008年 | 828篇 |
2007年 | 846篇 |
2006年 | 735篇 |
2005年 | 724篇 |
2004年 | 688篇 |
2003年 | 641篇 |
2002年 | 656篇 |
2001年 | 136篇 |
2000年 | 88篇 |
1999年 | 142篇 |
1998年 | 157篇 |
1997年 | 127篇 |
1996年 | 116篇 |
1995年 | 117篇 |
1994年 | 99篇 |
1993年 | 77篇 |
1992年 | 106篇 |
1991年 | 69篇 |
1990年 | 71篇 |
1989年 | 71篇 |
1988年 | 42篇 |
1987年 | 53篇 |
1986年 | 52篇 |
1985年 | 43篇 |
1984年 | 61篇 |
1983年 | 48篇 |
1982年 | 66篇 |
1981年 | 46篇 |
1980年 | 47篇 |
1979年 | 39篇 |
1978年 | 34篇 |
1977年 | 36篇 |
1976年 | 22篇 |
1975年 | 18篇 |
1974年 | 16篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
Véronique Blanchard Muriel Chritin Sheela Vyas Marc Savasta Claude Feuerstein Yves Agid France Javoy-Agid Rita Raisman-Vozari 《Journal of neurochemistry》1995,64(4):1669-1679
Abstract: The present study was undertaken to examine the adaptive changes occurring 1 and 6 months after moderate or severe unilateral 6-hydroxydopamine-induced lesions confined to the lateral part of the rat substantia nigra pars compacta (SNC). The expression of tyrosine hydroxylase (TH) enzyme was analyzed in the remaining dopaminergic nigral cell bodies and in the corresponding striatal nerve endings. In the cell bodies of the lesioned SNC, TH mRNA content was increased (+20 to +30%) 6 months after the lesion without changes in cellular TH protein amounts. The depletion of TH protein in the nerve terminal area was less severe than the percentage of cell loss observed in the SNC at 1- and 6-month postlesion intervals. Moreover, the decrease in TH protein in the ipsilateral striatum was less pronounced 6 months after lesion than 1 month after. That no corresponding change in TH protein content was observed in the cell bodies at a time when TH increased in nerve terminals suggests that the newly synthesized protein is probably rapidly transported to the striatal fibers. These results suggest the existence of a sequence of changes in TH expression between cell bodies and fibers, occurring spontaneously after partial denervation of the nigrostriatal pathway. 相似文献
92.
Marc Mathieu Christian Lehmann Alain Razaname Gabriele Tuchscherer 《Letters in Peptide Science》1997,4(2):95-100
The assembly of helical and -sheet peptide blocks containing reactive chain ends results inhighly branched chain architectures (locked-in folds) mimicking native tertiary structures.This molecular kit strategy allows to bypass the protein folding problem in protein de novodesign and gives access to protein mimetics of high thermodynamic stability. The validity ofthis concept is exemplified for the design and synthesis of locked-in folds mimicking the zincfinger and MHC folding motifs. 相似文献
93.
94.
95.
96.
Offshore fluxes of Cu, Zn, Cd, Pb and Hg were calculated based onresidual flow patterns and salinity gradients along the Belgian
coast. Theresidual flow lines along the Belgian coast are more or less parallel to thecoast except in the area where the north-easterly
flowing watermass comingfrom the Channel encounters the south-westerly-oriented Scheldt outflow,forming a residual hydrodynamical
front. From the steady-state salinitypattern, diffusion coefficients perpendicular to the residual flow werededuced; they
ranged from 21 to 108 m2 s-1.
Offshore fluxes of dissolved and particulate trace metals based on diffusiveand mixing processes are calculated. The steady
state profiles of dissolvedmetals show a dilution effect in the coastal waters, reaching an almostconstant concentration in
the marine watermass in the 1981–1983dataset. The ratios of the Scheldt input of trace metals to the totaldissolved offshore
flux vary from 38 to 55% (1981–1983),depending on the kind of metal, and from 55 to 91% (1995–1996).The ratio of the Scheldt
input to the dissolved metal flow parallel to thecoast, is in both periods (1981–1983 and 1995–1996), smallerthan 1%. The
steady-state concentration profiles of particular metalsversus salinity are fairly constant in the coastal-estuarine and marinewatermasses,
but decrease very abruptly from the first to the secondwatermass. Assuming a conservative behaviour of the particular metals,offshore
fluxes and the resulting concentration increases agree fairly wellwith the observed values. The ratios of the Scheldt input
to the particulatetrace metal offshore flux vary between 30 to 46% (1981–1983)and 13 to 37% (1995–1996). The contribution
of the Scheldtestuary to the flows parallel to the coast ranges from 1.6 to 2.9%(1981–1983) and from 0.6 to 1.6% (1995).
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
97.
The evolutionary relationship of muscle and nonmuscle actin isoforms in deuterostomia was studied by the isolation and characterization
of two actin genes from the cephalochordate Branchiostoma lanceolatum and two from the hemichordate Saccoglossus kowalevskii The Branchiostoma genes specify a muscle and a nonmuscle actin type, respectively. Together with earlier results on muscle actins from vertebrates
and urochordates, a N-terminal sequence signature is defined for chordate muscle actins. These diagnostic amino acid residues
separate the chordates from the echinoderms and other metazoa. Although the two Saccoglossus actins characterized so far lack the diagnostic residues, in line with the presumptive phylogenetic position of hemichordates
outside the chordates, a definitive conclusion can only be expected once the full complement of actin genes of Saccoglossus is established. Comparison of the intron patterns of the various deuterostomic actin genes shows that intron 330-3, which
is present in all vertebrate genes, is conspicuously absent from nonvertebrate genes. The possible origin of this intron is
discussed.
Received: 4 July 1997 / Accepted: 29 August 1997 相似文献
98.
NMDA Receptor Heterogeneity During Postnatal Development of the Rat Brain: Differential Expression of the NR2A, NR2B, and NR2C Subunit Proteins 总被引:17,自引:3,他引:14
Andreas Wenzel Jean Marc Fritschy Hanns Mohler Dietmar Benke 《Journal of neurochemistry》1997,68(2):469-478
Abstract: Changes in the expression of the NMDA receptor subunits (NRs) NR2A, 2B, and 2C were investigated in histo blots of the developing rat brain with subunit-specific antisera. At birth, the NR2B subunit was detected almost ubiquitously, the NR2A subunit staining was faint and restricted to the hippocampus, cerebral cortex, and striatum, and no NR2C subunit immunoreactivity was detected. During the first 3 postnatal weeks, the NR2B subunit became confined to forebrain structures, whereas the NR2A immunoreactivity became abundantly expressed throughout the brain. The NR2C immunoreactivity emerged 5 days after birth in the olfactory bulb, thalamus, and vestibular nuclei and became very intense after 10 days in cerebellar granule cells, its primary site of expression in adulthood. After 3 weeks, NR2A and NR2B immunoreactivity decreased to adult levels, whereas NR2C immunoreactivity remained unchanged. The patterns of distribution of the subunit proteins were in agreement with those of their corresponding mRNAs, as monitored by in situ hybridization histochemistry, although the mRNA translation appeared to be delayed by several days in certain areas. Our results reveal a progressive increase in the heterogeneity of NMDA receptors due to the comparably late onset of NR2A and NR2C subunit expression and by the area-specific rearrangement of NR2B subunit expression following birth. 相似文献
99.
Mitchell Gary F.; Pfeffer Marc A.; Finn Peter V.; Pfeffer Janice M. 《Journal of applied physiology》1997,82(1):203-210
Mitchell, Gary F., Marc A. Pfeffer, Peter V. Finn, andJanice M. Pfeffer. Comparison of techniques for measuringpulse-wave velocity in the rat. J. Appl.Physiol. 82(1): 203-210, 1997.We evaluatedmethods for measuring average and regional pulse-wave velocity alongthe full length of the aorta in 18-mo-old ether-anesthetized malespontaneously hypertensive rats. Catheter-tip manometers were placed inthe ascending and descending thoracic aorta via the right carotid andleft femoral arteries, respectively. As the distal catheter waswithdrawn at 1-cm intervals, the relationship between distal catheterinsertion distance and distance between transducers was determined fromthe intercept of the insertion distance vs. transmission delayregression line. Methods that assessed the foot-to-foot time delaybetween pressures accurately predicted the separation between catheters(measured distance of 14.3 cm; intercept of 14.0 ± 0.5 cm;P = not significant) were highlyreproducible (coefficient of variation of 2.3% for repeated measurements) and showed minimal variability (range 509 ± 30 to 600 ± 29 cm/s) along the full length of the aorta. Methods that madeuse of the pressure-pressure transfer function were spatially (range ofvalues along the aorta 367 ± 17 to 722 ± 39 cm/s) and temporally more variable, especially during vasoconstriction with methoxamine, due to the effects of reflected waves. 相似文献
100.
Marc Jarry Mohamed Khaladi Martine Hossaert-McKey Doyle McKey 《Acta biotheoretica》1995,43(1-2):53-65
A model is proposed for the population dynamics of an annual plant (Sesbania vesicaria) with a seed bank (i.e. in which a proportion of seeds remain dormant for at least one year). A simple linear matrix model is deduced from the life cycle graph. The dominant eigenvalue of the projection matrix is estimated from demographic parameters derived from field studies. The estimated values for population growth rate () indicates that the study population should be experiencing a rapid exponential increase, but this was not the case in our population.The addition of density dependent effects on seedling survivorship and adult fecundity, effects for which field studies provide evidence, considerably improves our model. Depending on the demographic parameters, the model leads to stable equilibrium, oscillations, or chaos. Study of the behaviour of this model in the parameter space shows that the existence of a seed bank allows higher among-year variation of adult fecundity, without leaving the region of demographic stability. Field data obtained over 3 years confirm this prediction. 相似文献