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151.
152.
Eighteen Adh-negative mutations were selected with 1-pentyn-3-ol after feeding of formaldehyde. Twelve of the 18 were shown by cytological and genetic analysis to be deletions. Cytological examination of the deletions allowed us to localize the Adh gene to a region including bands 35B3-5 on the left arm of chromosome 2. The deletions were also used to order known visible loci located near Adh.--The vital loci near Adh were also investigated. A total of 109 lethal mutations were generated with EMS and 33 of these, localized within a region defined by the overlap of two of the deletions, were found to belong to 13 complementation groups. If one includes three other loci known to belong there (el, Adh and Sco) a total of 16 complemetation groups have been identified in the region close to Adh. 相似文献
153.
154.
John L. McGregor Kenneth J. Clemetson Elizabeth James Ernst F. Luscher Marc Dechavanne 《生物化学与生物物理学报:生物膜》1980,599(2):473-483
Intact human blood platelets were radioactively labelled at the surface by techniques specific for proteins or glycoproteins. Labelled platelet samples were analyzed by a high-resolution two-dimensional separation system involving isoelectric focusing in the first dimension and discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis in the second. The major platelet membrane glycoprotein (GP) bands (Ib, IIb, IIIa and IIIb) were found to be highly heterogeneous even after removal of terminal sialic acid residues. Lactoperoxidase-catalyzed iodination of platelets showed that the major labelled proteins (Ib, IIb, IIIa and IIIb) had altered isoelectric points () and molecular weights after neuraminidase treatment. A number of membrane glycoproteins previously undetected by one-dimensional gel electrophoresis were demonstrated and good evidence provided that the major platelet surface proteins are glycosylated. 相似文献
155.
Detection of the Viral Sarcoma Gene Product in Cells Infected with Various Strains of Avian Sarcoma Virus and of a Related Protein in Uninfected Chicken Cells
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Joan S. Brugge Marc S. Collett Aleem Siddiqui Barbara Marczynska F. Deinhardt R. L. Erikson 《Journal of virology》1979,29(3):1196-1203
Genetic analyses have defined a single gene (src) as that portion of the avian sarcoma virus (ASV) genome which encodes the protein directly responsible for ASV-induced neoplastic transformation. We have recently identified the polypeptide product of the src gene of the Schmidt-Ruppin (SR) strain of ASV, a 60,000-dalton phosphoprotein designated pp60(src), and have further determined that pp60(src) acts as a protein kinase. Essential to the identification and characterization of the pp60(src) protein of SR-ASV was the use of serum (TBR serum) from rabbits bearing SR-ASV-induced tumors. TBR serum was, however, strain specific, recognizing pp60(src) from SR-ASV-transformed cells only. We report here that sera from marmosets bearing tumors induced by the Bryan or SR strains of ASV (TBM sera) contain antibody which precipitates the transforming gene product from cells transformed by the SR, Bryan, Prague, or Bratislava strains of ASV. In contrast, rabbits bearing tumors induced by either the Bratislava or Bryan strains of ASV, or hamsters with SR-ASV-induced tumors did not produce antibody to pp60(src) from any strain of ASV. The 60,000-dalton polypeptides immunoprecipitated with TBM serum from cells transformed by each of the above virus strains are phosphoproteins. One-dimensional peptide mapping by limited proteolysis revealed that the pp60(src) proteins are structurally very similar, but not identical. Furthermore, all of the viral pp60(src) proteins have an associated phosphotransferase activity. In addition to detecting the viral src proteins, TBM serum was able to immunoprecipitate an antigenically related protein from normal uninfected avian cells. 相似文献
156.
157.
Harald Riedl 《Plant Systematics and Evolution》1981,138(3-4):283-286
Cynoglossopsis somaliensis
H. Riedl, sp. nov., is described as a new species of the hitherto monotypic genusCynoglossopsis
Brand which is closest related toCynoglossum L. but has to be included inBoraginoideae-Eritrichieae from the way the nutlets are attached to the gynobasis. 相似文献
158.
Microbial ecologists attempting to describe community structures through the use of synthetic parameters face enormous difficulties.
These stem in part from the necessity of using standard taxonomic reference levels in a field where the species level is poorly
defined. This paper presents an attempt to obviate this problem. A “functional evenness” index (E) is defined using information measures; it is based directly on the characteristics of the bacteria, as determined, for example,
with the API 20B method. Comparisons of this index with classic structure indices, such as taxonomic evenness (Pielou) or
systematic dominance (Hulburt), show that it behaves like an evenness index, while bypassing the taxonomic study required
before computation of the classic indices. Its use is illustrated with samples of aerobic heterotrophic bacteria obtained
from brackish lagoon sediments.
We are grateful to our statistician colleagues, Dr. Yves Lepage from Université de Montréal and Dr. André Plante from Université
du Québec à Montréal. Both have contributed important elements to the discussion presented in this Appendix. 相似文献
159.
During pregnancy the decline in blood [glucose] does not result from the increased distribution space of glucose. The absolute rate of glucose turnover increases in late pregnancy in parallel with the rise in the mass of the conceptus. Nevertheless, glucose turnover per kg body wt. is not increased in late pregnancy, since the lower blood [glucose] decreases glucose utilization by maternal tissues. 相似文献
160.
The solution conformation of the ionophore Monensin in its free-acid form bears a close resemblance to that of its Na+ salt. The backbone is folded into a closed loop, and the pseudocyclic structure is shut by head-to-tail H bonding between the carboxylic function and the alcoholic functions of the last six-membered ring with the mediation of a water molecule. A mode of trapping is proposed and compared to features observed in some other membrane-active complexones. 相似文献