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排序方式: 共有148条查询结果,搜索用时 15 毫秒
81.
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Wimmer N Marano RJ Kearns PS Rakoczy EP Toth I 《Bioorganic & medicinal chemistry letters》2002,12(18):2635-2637
Synthesis of novel polycationic lipophilic peptide core(s) was accomplished and these agents successfully transfected human retinal pigment epithelium cells with ODN1 upon complexation with the oligonucleotide. The level of transfection was indirectly measured by the decreased production of the protein hVEGF (human vascular endothelial growth factor) in comparison to the transfection agent cytofectin GSV. 相似文献
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N Bhutiani CW Kimbrough NC Burton S Morscher M Egger K McMasters 《Biotechnic & histochemistry》2017,92(1):1-6
We introduce a new approach to detect individual microparticles that contain NIR fluorescent dye by multispectral optoacoustic tomography in the context of the hemoglobin-rich environment within murine liver. We encapsulated a near infrared (NIR) fluorescent dye within polystyrene microspheres, then injected them into the ileocolic vein, which drains to the liver. NIR absorption was determined using multispectral optoacoustic tomography. To quantitate the minimum diameter of microspheres, we used both colorimetric and spatial information to segment the regions in which the microspheres appear. Regional diameter was estimated by doubling the maximum regional distance. We found that the minimum microsphere size threshold for detection by multispectral optoacoustic tomography images is 78.9 µm. 相似文献
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A. V. Marano C. L. A. Pires-Zottarelli M. D. Barrera M. M. Steciow F. H. Gleason 《Hydrobiologia》2011,659(1):93-109
Leaf litter is a very important primary source of energy in woodland streams. Decomposition of leaf litter is a process mediated
by many groups of microorganisms which release extracellular enzymes for the degradation of complex macromolecules. In this
process, true fungi and straminipiles are considered to be among the most active groups, more active than the bacteria, at
least during the early stages of the process. Colonization increases the quality of the leaves as a food resource for detritivores.
In this way, matter and energy enter detritus-based food chains. Previously, aquatic hyphomycetes were considered to be the
major fungal group responsible for leaf litter decomposition. Although zoosporic fungi and straminipiles are known to colonize
and decompose plant tissues in various environments, there is scant information on their roles in leaf decomposition. This
study focuses on the communities of zoosporic fungi and straminipiles in a stream which are involved in the decomposition
of leaves of two plant species, Ligustrum lucidum and Pouteria salicifolia, in the presence of other groups of fungi. A characteristic community dominated by Nowakowskiella elegans, Phytophthora sp., and Pythium sp. was found. Changes in the fungal community structure over time (succession) was observed: terrestrial mitosporic fungi
appeared during the early stages, zoosporic fungi, straminipiles, and aquatic Hyphomycetes in early-to-intermediate stages,
while representatives of the phylum Zygomycota were found at early and latest stages of the decomposition. These observations
highlight the importance of zoosporic fungi and straminipiles in aquatic ecosystems. 相似文献
88.
José Ivanildo de Souza Agostina Virginia Marano Carmen Lidia Amorim Pires-Zottarelli Felipe Santiago Chambergo Ricardo Harakava 《Mycological Progress》2014,13(4):975-980
Cerrado (Brazilian savanna) areas present a high number of endemic and unknown species and consequently have been included among the world’s biodiversity hotspots. After a survey of zygomycetes from Cerrado areas of the “Reserva Biológica de Mogi Guaçu” (RBMG) in São Paulo State, we have found a Mucor-like fungus that produces very large sporangiospores and clusters of giant cells visible to the naked-eye. This isolate was characterized morphologically, based on partial LSU (28S) and complete ITS rDNA sequences. Maximum likelihood phylogenetic analyses support its inclusion within the recently emended genus Backusella. 相似文献
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Ozgur E Akman James CW Locke Sanyi Tang Isabelle Carré Andrew J Millar David A Rand 《Molecular systems biology》2008,4(1)
A striking and defining feature of circadian clocks is the small variation in period over a physiological range of temperatures. This is referred to as temperature compensation, although recent work has suggested that the variation observed is a specific, adaptive control of period. Moreover, given that many biological rate constants have a Q10 of around 2, it is remarkable that such clocks remain rhythmic under significant temperature changes. We introduce a new mathematical model for the Neurospora crassa circadian network incorporating experimental work showing that temperature alters the balance of translation between a short and long form of the FREQUENCY (FRQ) protein. This is used to discuss period control and functionality for the Neurospora system. The model reproduces a broad range of key experimental data on temperature dependence and rhythmicity, both in wild‐type and mutant strains. We present a simple mechanism utilising the presence of the FRQ isoforms (isoform switching) by which period control could have evolved, and argue that this regulatory structure may also increase the temperature range where the clock is robustly rhythmic. 相似文献