全文获取类型
收费全文 | 12682篇 |
免费 | 872篇 |
专业分类
13554篇 |
出版年
2024年 | 16篇 |
2023年 | 96篇 |
2022年 | 95篇 |
2021年 | 192篇 |
2020年 | 166篇 |
2019年 | 210篇 |
2018年 | 415篇 |
2017年 | 410篇 |
2016年 | 555篇 |
2015年 | 748篇 |
2014年 | 726篇 |
2013年 | 1001篇 |
2012年 | 1251篇 |
2011年 | 1138篇 |
2010年 | 654篇 |
2009年 | 515篇 |
2008年 | 747篇 |
2007年 | 704篇 |
2006年 | 682篇 |
2005年 | 580篇 |
2004年 | 571篇 |
2003年 | 510篇 |
2002年 | 462篇 |
2001年 | 119篇 |
2000年 | 109篇 |
1999年 | 90篇 |
1998年 | 68篇 |
1997年 | 45篇 |
1996年 | 52篇 |
1995年 | 49篇 |
1994年 | 27篇 |
1993年 | 35篇 |
1992年 | 45篇 |
1991年 | 41篇 |
1990年 | 40篇 |
1989年 | 35篇 |
1988年 | 31篇 |
1987年 | 20篇 |
1986年 | 32篇 |
1985年 | 33篇 |
1984年 | 40篇 |
1983年 | 20篇 |
1982年 | 21篇 |
1981年 | 20篇 |
1980年 | 17篇 |
1979年 | 18篇 |
1978年 | 13篇 |
1977年 | 15篇 |
1976年 | 14篇 |
1974年 | 14篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
31.
Ana María Planchuelo 《Brittonia》1996,48(2):263-265
Lupinus rubriflorus Planchuelo, from Rio Grande do Sul, Brazil, is described and illustrated. This species is related toL. bracteolaris s.l. differing in pubescence, stipules, and floral characters. 相似文献
32.
The distribution of Keratella species from 15 different lakes in North Patagonia (Argentina) was analysed. The genus was not present at altitudes above 1000 m. K. tropica was restricted to Patagonian Plateau lakes with a comparatively high conductivity. A morphometric analysis of the widely distributed K. cochlearis was performed. Results showed three groups of K. cochlearis corresponding to Andean lakes, Patagonian Plateau lakes and a Patagonian Reservoir. 相似文献
33.
Sandra Irene Pitta-Alvarez Ana María Giulietti 《In vitro cellular & developmental biology. Plant》1995,31(4):215-220
Summary
Brugmansia candida hairy roots, obtained by infection withAgrobacterium rhizogenes LBA 9402, exhibit, after subculturing in liquid media, a tendency towards dedifferentiation. It has been found that the following
strategies can be applied to inhibit this dedifferentiation and preserve normal root morphology: (a) lowering both the mineral
and sucrose concentration in the media employed so as to diminish osmotic stress (a condition to which these roots appear
to be particularly susceptible); (b) employing antiauxins in appropriate concentrations; and (c) maintaining the hairy roots
on solid media prior to use in production processes in liquid media. The first strategy suggested does not favor alkaloid
productivity, but in this case a two-step method could be attempted: biomass with normal root morphology could be obtained
in a first stage using low sucrose concentrations, and in a second stage, sucrose could be increased in order to achieve higher
productivity. In all the clones ofB. candida obtained, alkaloid production was biased towards scopolamine. 相似文献
34.
35.
Summary Partition of purified horseradish peroxidase isoenzymes in aqueous two-phase systems was not affected by pH changes, but tie-line length and NaCl addition greatly increased the partition coefficient of all three isoenzymes, the former having more influence than the latter. In all systems, K were higher for acidic than those for neutral and basic isoenzymes, and K for basic were the lowest. 相似文献
36.
Jesús Manuel de la Fuente Amalia Vázquez M. Mar González Miguel Sánchez María Molina César Nombela 《Applied microbiology and biotechnology》1993,38(6):763-769
Thermosensitive mutant strains of Saccharomyces cerevisiae that fail to generate an osmotically stable cell wall when grown at a non-permissive temperature release their cell contents upon expression of the mutation. Therefore, they may represent an alternative for the production of homologous or heterologous protein preparations. In order to analyse the expression of two of these mutations, lyt2 and slt2, we grew the corresponding strains under precisely defined conditions in batch and continuous fermentors. A switch in the temperature of batch cultures from 24° C to 37° C determined lysis of the cells with a significant release of intracellular enzymes. These include alkaline phosphatase and periplasmic proteins such as glucan-degrading enzymes, the pattern of cell lysis and protein release being maintained for about 6 h. One-stage continuous cultures of a lyt2 mutant were maintained for long periods at 37° C; a fraction of the population lysed and released the indicated proteins, but eventually a revertant of the lytic phenotype was selected. To avoid this, a two-stage continuous culture system was developed by connecting two fermentors in series, the effluent from the first one at 24°C being fed to the second one adjusted to 37° C. A steady state of cell lysis and protein liberation was reached in the second-stage fermentor without any evidence of selection of revertants. This system can be very useful for developing conditions for the use of yeast strains to produce protein preparations.
Correspondence to: C. Nombela 相似文献
37.
38.
InNeurospora crassa thenit-2 andnmr-1 (ms-5) loci represent the major control genes encoding regulatory proteins that allow the coordinated expression of various systems involved with the utilization of a secondary nitrogen source. In this paper we examine the effect of thenit-2 andms-5 (nmr-1 locus) mutations on the regulation of the ammonium assimilation enzymes, glutamine synthetase and glutamate dehydrogenase, which are regulated by the products of these genes; however, glutamate synthase is not so regulated. Glutamine synthetase and glutamate dehydrogenase levels are also regulated by the amino nitrogen content. We present evidence that thems-5 andgln r strains, which behave very similarly in their resistance to glutamine repression, are different and map in different loci. 相似文献
39.
Mayrel Palestino-Domínguez Alejandro Escobedo-Calvario Soraya Salas-Silva Moises Vergara-Mendoza Veronica Souza-Arroyo Roberto Lazzarini Roxana Miranda-Labra Leticia Bucio-Ortiz María Concepción Gutiérrez-Ruiz Luis E. Gomez-Quiroz 《Journal of biochemical and molecular toxicology》2023,37(4):e23302
Alcohol-induced pancreas damage remains as one of the main risk factors for pancreatitis development. This disorder is poorly understood, particularly the effect of acetaldehyde, the primary alcohol metabolite, in the endocrine pancreas. Hepatocyte growth factor (HGF) is a protective protein in many tissues, displaying antioxidant, antiapoptotic, and proliferative responses. In the present work, we were focused on characterizing the response induced by HGF and its protective mechanism in the RINm5F pancreatic cell line treated with ethanol and acetaldehyde. RINm5F cells were treated with ethanol or acetaldehyde for 12 h in the presence or not of HGF (50 ng/ml). Cells under HGF treatment decreased the content of reactive oxygen species and lipid peroxidation induced by both toxics, improving cell viability. This effect was correlated to an improvement in insulin expression impaired by ethanol and acetaldehyde. Using a specific inhibitor of Erk1/2 abrogated the effects elicited by the growth factor. In conclusion, the work provides mechanistic evidence of the HGF-induced-protective response to the alcohol-induced damage in the main cellular component of the endocrine pancreas. 相似文献
40.
Rhodobacter capsulatus E1F1 showed high cytosolic urease activity when growing on urea, purines, and purine metabolites as nitrogen source. Molecular mass ofR. capsulatus enzyme is similar to that of other bacteria and greatly differs from that of jack bean. Kinetic parameters of partially purifiedR. capsulatus enzyme resemble those described in other bacterial ureases. The activity was inhibited by metal-chelating agents and by mercurials. Urease fromR. capsulatus E1F1 was negligible in nitrogen-starved cells or in cells cultured with nitrate, ammonium, or amino acids. Moreover, ammonium inhibited both the urea uptake and the urease activity expression inR. capsulatus cells. 相似文献