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91.
内毒素致大鼠肺损伤时肺组织β受体的变化与膜磷脂代谢的关系 总被引:1,自引:0,他引:1
采用放射性配基结合分析法,现察内毒大致大鼠急性肺损伤时肺β-肾上腺素能受体(β-AR)的变化。分别用荧光偏振法和高效液相色谱测定肺组织细胞膜脂流动性和磷脂含量。结果显示:(1)静脉注射内毒素后4h,大鼠肺β-AR的最大结合容量明显降低,较对照组减少47%;(2)内毒素组肺膜脂流动性和磷脂含量均明显降低,同时伴有肺组织磷脂酶A2(PLA2)活性升高。提示:(1)β-AR下调导致其介导的功能减弱,在内毒素诱导的大鼠急性肺损伤发病机理中起一定作用;(2)PLA2激活是膜磷脂减少的重要原因,后者可导致膜脂流动性降低,结果引起β-AR的侧向扩散和旋转运劝减弱,从而减少β-AR与配基结合的机率,出现β-AK下调。 相似文献
92.
观察了hFPIL6/2对6.5Gyγ线照射NIH小鼠第10天造血功能恢复的影响。结果表明:照射小鼠连续4d给予hFPIL6/2250μg·kg-1·d-1,其脾重、CFU-8、骨髓有核细胞数及CM-CFU分别比对照组增加59.0%、278.5%、57.9%和138.2%,统计学处理均有显著差异;对此四项指标的改善也明显优于25μg组。另外,250μg剂量组小鼠外周血象30d的动态观察结果表明,hFPIL6/2不但能明显提高红细胞和血红蛋白的最低值,而且能使血小板的恢复提前。提示hFPIL6/2在促进血小板生成和促进红系造血方面可能具有良好的应用前景。 相似文献
93.
Morphogenetic Rescue of Rhizobium meliloti Nodulation Mutants by trans-Zeatin Secretion 总被引:5,自引:0,他引:5 下载免费PDF全文
The development of nitrogen-fixing nodules is induced on the roots of legume host plants by Rhizobium bacteria. We employed a novel strategy to probe the underlying mechanism of nodule morphogenesis in alfalfa roots using pTZS, a broad host range plasmid carrying a constitutive trans-zeatin secretion (tzs) gene from Agrobacterium tumefaciens T37. This plasmid suppressed the Nod- phenotype of Rhizobium nodulation mutants such that mutants harboring pTZS stimulated the formation of nodulelike structures. Alfalfa roots formed more or fewer of these nodules according to both the nitrogen content of the environment and the position along the root at which the pTZS+ bacteria were applied, which parallels the physiological and developmental regulation of true Rhizobium nodule formation. This plasmid also conferred on Escherichia coli cells the ability to induce root cortical cell mitoses. Both the pattern of induced cell divisions and the spatially restricted expression of an alfalfa nodule-specific marker gene (MsENOD2) in pTZS-induced nodules support the conclusion that localized cytokinin production produces a phenocopy of nodule morphogenesis. 相似文献
94.
Shu-Miaw Chaw Huei Long Bin-Shin Wang Andrey Zharkikh Wen-Hsiung Lie 《Journal of molecular evolution》1993,37(6):624-630
The evolutionary position of the yew family, Taxaceae, has been very controversial. Some plant taxonomists strongly advocate excluding Taxaceae from the conifer order and raising its taxonomic status to a new order or even class because of its absence of seed cones, contrary to the case in the majority of conifers. However, other authors believe that the Taxaceae are not fundamentally different from the rest of the conifers except in that they possess the most reduced solitary-ovule cones. To resolve the controversy, we have sequenced the 18S rRNA genes from representative gymnosperms: Taxus mairei (Taxaceae), Podocarpus nakaii (Podocarpaceae), Pinus luchuensis (Pinaceae), and Ginkgo biloba (Ginkgoales). Our phylogenetic analysis of the new sequence data with the published 18S rRNA sequence of Zamia pumila (a cycad) as an outgroup strongly indicates that Taxus, Pinus, and Podocarpus form a monophyletic group with the exclusion of Ginkgo and that Taxus is more closely related to Pinus than to Podocarpus. Therefore, Taxaceae should be classified as a family of Coniferales. Our finding that Taxaceae, Pinaceae, and Podocarpaceae form a clade contradicts both the view that the uniovulate seed of Taxaceae is a primitive character and the view that the Taxaceae are descendants of the Podocarpaceae. Rather, the uniovulate seed of Taxaceae and that of some species of Podocarpus appear to have different origins, probably all reduced from multiovulate cones.
Correspondence to: W.-H. Li 相似文献
95.
The early steps of symbiotic nodule formation by Rhizobium on plants require coordinate expression of several nod gene operons, which is accomplished by the activating protein NodD. Three different NodD proteins are encoded by Sym plasmid genes in Rhizobium meliloti, the alfalfa symbiont. NodD1 and NodD2 activate nod operons when Rhizobium is exposed to host plant inducers. The third, NodD3, is an inducer-independent activator of nod operons. We previously observed that nodD3 carried on a multicopy plasmid required another closely linked gene, syrM, for constitutive nod operon expression. Here, we show that syrM activates expression of the nodD3 gene, and that nodD3 activates expression of syrM. The two genes constitute a self-amplifying positive regulatory circuit in both cultured Rhizobium and cells within the symbiotic nodule. We find little effect of plant inducers on the circuit or on expression of nodD3 carried on pSyma. This regulatory circuit may be important for regulation of nod genes within the developing nodule. 相似文献
96.
The maximum quantum yields (a,c) for CO2 uptake in low-oxygen atmospheres were determined for 11 species of C3 vascular plants of diverse taxa, habitat and life form using an Ulbricht-sphere leaf chamber. Comparisons were also made between tissues of varied age within species. The species examined were Psilotum nudum (L.) P. Beauv., Davallia bullata Wall. ex Hook., Cycas revoluta Thunb., Araucaria heterophylla (Salisb.) Franco, Picea abies (L.) Karst., Nerium oleander L., Ruellia humilis Nutt., Pilea microphylla (L.) Karst., Beaucarnea stricta Lem., Oplismenus hirtellus (L.) P. Beauv. and Poa annua L. Quantum yields were calculated from the initial slopes of the response of CO2 uptake to the quantity of photons absorbed in conditions of diffuse lighting. Regression analysis of variance of the initial slopes of the response of CO2 uptake to photon absorption failed to show any statistically significant differences between age classes within species or between the mature photosynthetic organs of different species. The constancy of a,c was apparent despite marked variation in the light-saturated rates of CO2 uptake within and between species. The mean a,c was 0.093±0.003 for 11 species. By contrast, surface absorptance varied markedly between species from 0.90 to 0.60, producing proportional variation in the quantum yield calculated on an incidentlight basis. The ratio of variable to maximum fluorescence emission at 695 nm for the same tissues also failed to show any statistically significant variation between species, with a mean of 0.838±0.008. Mean values of a,c reported here for C3 species, in the absence of photorespiration, are higher than reported in previous surveys of vascular plants, but consistent with recent estimates of the quantum yields of O2 evolution.Abbreviations and Symbols A
rate of CO2 uptake per unit projected area (mol · m–2 · s–1)
- Fm
the maximum fluorescence emission at 695 nm in saturating excitation light when closure of PSII reaction centres is maximal (relative units)
- Fo
the ground fluorescence at 695 nm when all PSII reaction centres are assumed open (relative units)
- Fv
the difference between Fm and Fo
- JQ
rate of CO2 uptake by the sample (nmol · s–1)
- JQ
rate of photon absorption by the sample (nmol · s–1)
- Q
absorbed photon flux per unit of projected area (nmol · m–2 · s–1)
- 1
the light absorptance of photosynthetic organs (dimensionless)
- s1 and s'1
the total and projected surface areas of the photosynthetic organs examined (m2)
- a,c and i,c
the quantum yields for CO2 uptake on an absorbed- and incident-light basis, respectively (dimensionless)
- a,o
the quantum yield for O2 evolution on an absorbed-light basis (dimensionless)
This work was supported by grant PI7179-BIO, FWF, Austria to H.B-N. and by a British Council travel award to S.P.L. This work was completed under the auspices of U.S. Department of Energy under Contract No. DE-AC02-76CH00016. We also thank Dr. K.J. Parkinson of PP Systems, Hitchin, UK for the loan of a prototype of a commercial integrating-sphere leaf chamber developed from our design. 相似文献
97.
以草鱼ZC7901细胞株为材料,观察鱼类细胞从间期染色质到中期染色体的包装过程。主要通过(1)分裂期与间期细胞融合,诱导染色体早熟凝集;(2)染色体“伸长”处理;(3)培养细胞的低渗处理;(4)染色质辅展等方法,制作染色体标本,进行扫描和透射电镜观察。观察表明,鱼类染色质的基本结构与哺乳类细胞相同,也是直径约10nm的核丝。染色体的色装有两种形式:一种是多级螺旋化形成直径约300nm的染色单体, 相似文献
98.
99.
棉花枯萎病菌多聚半乳糖醛酸内切酶在pH大于7时不稳定,故对它进行多种化学修饰而又不影响其活性,必须在pHd小于7的体系中进行。本文报道将PGAUase在还原剂存在下,与稀酸处理的Sepharose 4B交联,获得较高活力的固定化酶。固定化酶催化动力学表明,最适pH为4,4,最适温度为55℃,在pH1至8.0范围内稳定。和溶液酶比较,对热稳定性提高,但对碱稳定性下降。以多聚半乳糖醛酸为底物,Km为0.27mmol/L,Vmax为66.67nmol/L·min,均大于溶液酶(Km=0.07mmol/L,Vmax=28.00nmol/L·min)。在pH4.8,30℃,聚半乳糖醛酸在固相酶的柱中循环水解不同的时间降解产物经圆盘电泳和等电聚焦测定,得到不同大小的寡糖片段混合物,证明固相酶和溶液酶的作用方式相同,同时使以酶解法制备一定大小的有生物活性的寡糖分子成为可能。 相似文献
100.
Yuheng Chen Yongbin Wu Yuran Dong Yao Li Zhiwei Ge Oduro George Gang Feng Lingfeng Mao 《Diversity & distributions》2023,29(2):232-243