Steroid anaesthesia: alphadione depresses multiunit activity in the mesencephalic reticular formation

Cortical EEG and multiunit activity (MUA) of the mesencephalic reticular formation (MRF), area hypothalami anterior (AH) and the nucleus amygdalae basalis (AMY) were studied before and after different doses of alphadione (Althesin) and hexobarbitone (Evipan-Natrium) given to cats with chronically implanted electrodes. Non-anaesthetic doses of alphadione (0.15 ml/kg; 0.3 ml/kg; 0.6 ml/kg and 1.2 ml/kg i.p.) had sedative effects decreasing selectively the MUA in the MRF. In doses of 2.0 ml/kg, 2.4 ml/kg and 3.0 ml/kg i.p., alphadione induced anaesthesia which was associated with a rapid decrease of MUA in the MRF and by a gradual decrease of activity in the AH and AMY. The i.p. dose of 3.0 ml/kg abolished MUA responses of the reticular formation to acoustic, visual and somatic stimulation but failed to block responses to pain. Deep anaesthesia with lasting analgesia could be maintained by i.v. infusion (0.075 ml/kg/min). This procedure blocked the responsiveness to painful stimulation while pharyngeal and laryngeal reflexes were maintained. Hexobarbitone in a dose of 20.0 mg/kg i.p. did not produce anaesthesia in the cat. Administration of 40.0 mg/kg i.p. resulted in a rapid decrease of MUA in the MRF, AH and AMY, MUA responses to each stimulation were abolished and the pharyngeal reflex was blocked.     

Identification of guanine and adenine adducts in DNA alkylated by dibromodulcitol in vitro and in vivo

DNA reacted with dibromodulcitol in neutral solution yielded 3- and 7-alkyl substituted purines after hydrolysis at neutral pH-value at 37°C. The alkylated products were identified by mass spectrometry and by comparison of their UV absorption spectra and chromatographic properties on thin-layer chromatography (TLC) and various columns with those of the corresponding galactitylpurine derivatives obtained by synthetic route from alkylation of the appropriate nucleic bases or nucleosides. The labelled alkylpurines occurring in DNA of Yoshida tumour cells treated with [³H]dibromodulcitol in vivo were also indentified by co-chromatography of labelled DNA hydrolysate with synthetic 3- and 7-alkyl substituted purines. On the basis of the same chromatographic behaviour 3-(1-deoxy-3,6-anhydrogalactit-1-yl)adenine, 7-(1-deoxygalactit-1-yl)guanine, 7-(1-deoxy-3,6-anhydrogalactit-1-yl)guanine and 1,6-di(guanin-7-yl)-1,6-dideoxygalactitol were identified as main alkylated products in tumor cell DNA after in vivo treatment with dibromodulcitol.     

A propos de quelques groupements végétaux observés entre monaco et gênes

Résumé Le climat chaud et humide de la Ligurie méditerranéenne détermine la réapparition de quelques groupements végétaux connus de la Catalogne et rares ou nuls en Languedoc: Inulo-Oryzopsietum miliaceae, Cichorio-Sporoboletum poiretii, Hyparrhenietum hirto-pubescentis, Rubo-Coriarietum.Le caractère particulier de la végétation ligure a été exprimé par des groupements spéciaux: Pistacio-Rhamnetum alaterni, qui remplace le Quercetum cocciferae, groupements à Ostrya carpinifolia et à Brachypodium pinnatum des endroits frais, groupements buissonnants à Erica arborea et Genista germanica, à Rosmarinus et Genista cinerea, Asplenio-Campanuletum macrorhizae des rochers, etc.

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Summary The warm and humid climate of the Mediterranean Liguria determines the reappearance of some communities, known in Catalonia, and seldom or not existing in Languedoc: Inulo-Oryzopsietum miliaceae, Cichorio-Sporoboletum poiretii, Hyparrhenietum hirto-pubescentis, Rubo-Coriarietum.The particular character of the Liguric vegetation is expressed in special communities: Pistacio-Rhamnetum alaterni, which replaces the Quercetum cocciferae, communities with Ostrya carpinifolia and with Brachypodium pinnatum in the fresh places, bushy communities with Erica arborea and Genista germanica, with Rosmarinus and Genista cinerea, Asplenio-Campanuletum macrorhizae of the rocks, and so on.

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Zusammenfassung Das warme und feuchte Klima der mediterranen Ligurie bestimmt das Wiedererscheinen einiger Pflanzengesellschaften, die in Katalonien bekannt sind, aber selten oder nicht in Languedoc vorkommen: Inulo-Oryzopsietum miliaceae, Cichorio-Sporoboletum poiretii, Hyparrhenietum hirto-pubescentis, Rubo-Coriarietum.Der eigenartige Charakter der ligurischen Vegetation ist in besonderen Pflanzengesellschaften ausgedrückt: Pistacio-Rhamnetum alaterni, das das Quercetum cocciferae ersetzt, Gesellschaften mit Ostrya carpinifolia und mit Brachypodium pinnatum der frischen Standorte, Erica arborea-Genista germanica-Silikatbodengebüsche, Rosmarinus-Genista cinerea-Kalkbodengebüsche, Asplenio-Campanuletum macrorhizae der Felsen usw.