全文获取类型
收费全文 | 809篇 |
免费 | 59篇 |
国内免费 | 1篇 |
专业分类
869篇 |
出版年
2023年 | 9篇 |
2022年 | 9篇 |
2021年 | 26篇 |
2020年 | 19篇 |
2019年 | 15篇 |
2018年 | 26篇 |
2017年 | 33篇 |
2016年 | 43篇 |
2015年 | 30篇 |
2014年 | 50篇 |
2013年 | 52篇 |
2012年 | 47篇 |
2011年 | 48篇 |
2010年 | 34篇 |
2009年 | 31篇 |
2008年 | 25篇 |
2007年 | 34篇 |
2006年 | 20篇 |
2005年 | 22篇 |
2004年 | 31篇 |
2003年 | 29篇 |
2002年 | 23篇 |
2001年 | 16篇 |
2000年 | 14篇 |
1999年 | 7篇 |
1998年 | 6篇 |
1995年 | 4篇 |
1994年 | 8篇 |
1992年 | 7篇 |
1991年 | 11篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 10篇 |
1987年 | 7篇 |
1986年 | 11篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 9篇 |
1981年 | 8篇 |
1980年 | 6篇 |
1979年 | 10篇 |
1978年 | 4篇 |
1976年 | 4篇 |
1975年 | 4篇 |
1974年 | 7篇 |
1973年 | 4篇 |
1969年 | 4篇 |
1968年 | 6篇 |
1966年 | 5篇 |
排序方式: 共有869条查询结果,搜索用时 0 毫秒
91.
Peña-Diaz J Akbari M Sundheim O Farez-Vidal ME Andersen S Sneve R Gonzalez-Pacanowska D Krokan HE Slupphaug G 《Journal of molecular biology》2004,342(3):787-799
Enzymes involved in genomic maintenance of human parasites are attractive targets for parasite-specific drugs. The parasitic protozoan Trypanosoma cruzi contains at least two enzymes involved in the protection against potentially mutagenic uracil, a deoxyuridine triphosphate nucleotidohydrolase (dUTPase) and a uracil-DNA glycosylase belonging to the highly conserved UNG-family. Uracil-DNA glycosylase activities excise uracil from DNA and initiate a multistep base-excision repair (BER) pathway to restore the correct nucleotide sequence. Here we report the biochemical characterisation of T.cruzi UNG (TcUNG) and its contribution to the total uracil repair activity in T.cruzi. TcUNG is shown to be the major uracil-DNA glycosylase in T.cruzi. The purified recombinant TcUNG exhibits substrate preference for removal of uracil in the order ssU>U:G>U:A, and has no associated thymine-DNA glycosylase activity. T.cruzi apparently repairs U:G DNA substrate exclusively via short-patch BER, but the DNA polymerase involved surprisingly displays a vertebrate POLdelta-like pattern of inhibition. Back-up UDG activities such as SMUG, TDG and MBD4 were not found, underlying the importance of the TcUNG enzyme in protection against uracil in DNA and as a potential target for drug therapy. 相似文献
92.
Hybrids in divers (Gaviiformes) 总被引:1,自引:0,他引:1
C. S. Roselaar Tineke G. Prins Mansour Aliabadian Vincent Nijman 《Journal of Ornithology》2006,147(1):24-30
The incidence of hybridisation in birds differs greatly between orders and is expected to be low in orders comprising few
species. The divers or loons (Gaviiformes) are a species-poor group in which apparent hybridisation has been reported infrequently.
Here we report on a hybrid diver stored in the collections of the Zoological Museum Amsterdam. The bird shows a heterogeneous
set of characters, some shared with the putative parent species White-billed Diver Gavia adamsii and Great Northern Diver G. immer, others being intermediate between the two. A Canonical Discriminant Function analysis positions the bird between these two
putative parent species, making a hybrid status quite likely. We evaluate the evidence for hybridisation in the order Gaviiformes
and conclude that hybridisation has been suspected in four of the five species, though documentation is limited. If this high
incidence could be confirmed, it would rank among the highest of any avian order, contradicting the assumption that incidence
of hybridisation in small orders is relatively low. 相似文献
93.
94.
Hosseini Tafreshi SA Shariati M Mofid MR Khayam Nekui M Esmaeili A 《Molecular biology reports》2012,39(3):2169-2178
VIGS (virus induced gene silencing) is considered as a powerful genomics tool for characterizing the function of genes in
a few closely related plant species. The investigations have been carried out mainly in order to test if a pre-existing VIGS
vector can serve as an efficient tool for gene silencing in a diverse array of plant species. Another route of investigation
has been the constructing of new viral vectors to act in their hosts. Our approach was the creation of a heterologous system
in which silencing of endogenous genes was achieved by sequences isolated from evolutionary remote species. In this study,
we showed that a TRV-based vector cloned with sequences from a gymnosperm, Taxus baccata L. silenced the endogenous phytoene desaturase in an angiosperm, N. benthamiana. Our results showed that inserts of between 390 and 724 bp isolated from a conserved fragment of the Taxus PDS led to silencing of its homolog in tobacco. The real time analysis indicated that the expression of PDS was reduced 2.1- to 4.0-fold in pTRV-TbPDS infected plants compared with buffer treated plants. Once the best insert is identified and the conditions are optimized
for heterologous silencing by pTRV-TbPDS in tobacco, then we can test if TRV can serve as an efficient silencing vector in Taxus. This strategy could also be used to silence a diverse array of genes from a wide range of species which have no VIGS protocol.
The results also showed that plants silenced heterologously by the VIGS system a minimally affected with respect to plant
growth which may be ideal for studying the genes that their complete loss of function may lead to decrease of plant growth
or plant death. 相似文献
95.
Olfa Abida Riadh Ben Mansour Bochra Gargouri Mourad Ben Ayed Abderrahmen Masmoudi Hamida Turki Hatem Masmoudi Saloua Lassoued 《Biological trace element research》2012,150(1-3):74-80
Pemphigus is an autoimmune disorder resulting from the interaction between autoantibodies and desmoglein. Oxidative stress seems to be responsible for the onset/aggravation of many human diseases. Actually, it is considered as one of the several factors for the etiopathogenesis of pemphigus. The present study aims to evaluate the oxidative state in the sera of pemphigus vulgaris and pemphigus foliaceus patients by assessing lipid peroxidation, proteins oxidation, and antioxidant enzyme activity. This study included 36 pemphigus vulgaris and 42 pemphigus foliaceus patients as well as a group of controls consisting of 78 healthy volunteers. Malondialdehyde levels (p?<?0.001) and catalase activity (p?<?0.001) are higher in both groups of patients than in the control group. The two groups of patients showed a nonsignificant decrease in the thiol groups compared with the healthy one. A nonsignificant difference was shown between pemphigus vulgaris and pemphigus foliaceus patients, except for the catalase which shows an increase in the pemphigus vulgaris group. We have also found significant correlations between serum oxidative stress marker levels and serum anti-desmoglein antibody levels in the two pemphigus groups. These findings underline the implication of oxidative stress in the physiopathology of pemphigus by the increase in the autoantibodies?? reactivity. 相似文献
96.
DF Henriques JA Quaresma HT Fuzii MR Nunes EV Silva VL Carvalho LC Martins SM Casseb JO Chiang PF Vasconcelos 《Memórias do Instituto Oswaldo Cruz》2012,107(5):630-636
Rocio virus (ROCV) is an encephalitic flavivirus endemic to Brazil. Experimental flavivirus infections have previously demonstrated a persistent infection and, in this study, we investigated the persistence of ROCV infection in golden hamsters (Mesocricetus auratus). The hamsters were infected intraperitoneally with 9.8 LD50/0.02 mL of ROCV and later anaesthetised and sacrificed at various time points over a 120-day period to collect of blood, urine and organ samples. The viral titres were quantified by real-time-polymerase chain reaction (qRT-PCR). The specimens were used to infect Vero cells and ROCV antigens in the cells were detected by immunefluorescence assay. The levels of antibodies were determined by the haemagglutination inhibition technique. A histopathological examination was performed on the tissues by staining with haematoxylin-eosin and detecting viral antigens by immunohistochemistry (IHC). ROCV induced a strong immune response and was pathogenic in hamsters through neuroinvasion. ROCV was recovered from Vero cells exposed to samples from the viscera, brain, blood, serum and urine and was detected by qRT-PCR in the brain, liver and blood for three months after infection. ROCV induced histopathological changes and the expression of viral antigens, which were detected by IHC in the liver, kidney, lung and brain up to four months after infection. These findings show that ROCV is pathogenic to golden hamsters and has the capacity to cause persistent infection in animals after intraperitoneal infection. 相似文献
97.
98.
ABSTRACT: This study progresses in the direction of identifying component(s) from the Mediterranean sponge, Spongia officinalis with anticonvulsant and analgesic activities. We investigated the efficacy of crude extract and its semi-purified fractions (F1-F3) of the defensive secretion from Spongia officinalis for their in vivo anticonvulsant activity using the pentylenetetrazole (PTZ) seizure model and analgesic activity using the writhing test in mice. Among the series the crude extract exhibited interesting analgesic activity in a dose dependent manner. Similarly the fraction F2 showed a partial protection of mice from PTZ-induced seizure and interesting analgesic activity in a dose dependent manner. The purification and the determination of chemical structure(s) of compound(s) of this active fraction are under investigation. 相似文献
99.
100.
Nasereddine Hamadi Ahmed Mansour Memy H. Hassan Fatima Khalifi‐Touhami Osama Badary 《Journal of biochemical and molecular toxicology》2012,26(10):384-392
The objective of this study was to investigate the ameliorative property and potential mechanism of resveratrol (RVT) in a dose of 10 mg/kg for 15 consecutive days against liver injury in streptozotocin‐induced diabetic rats. Diabetic rats significantly (P < 0.05) exhibited liver injury manifested by increased aspartylaminotransferase, alanine aminotransferase, and bilirubin; disturbed liver weight to body weight; and confirmed by hematoxylin and eosin staining. Liver from diabetic rats exhibited significant increase in malondialdehyde level and significant decrease in reduced glutathione, glutathione‐S‐transferase, quinone reductase, catalase, and superoxide dismutase. Diabetic rats showed significant disturbance in serum lipid profile. Treatment with RVT significantly (P < 0.05) abrogated diabetes‐induced perturbation in these parameters and liver histology. These data suggest that RVT treatment is associated with promising hepatoprotective effect against diabetes‐induced liver damage via reduction of serum glucose level and oxidative damage and improving serum lipid profile. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:384–392, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21432 相似文献