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1.
Atherosclerosis and its consequences remain prevalent clinical challenges throughout the world. Initiation and progression of atherosclerosis involves a complex, dynamic interplay among inflammation, hyperlipidemia, and endothelial dysfunction. A multicomponent treatment approach targeted for delivery within diseased vessels could prove beneficial in treating atherosclerosis. This study was undertaken to evaluate the multimodal effects of a novel ω-3-fatty acid-rich, 17-β-estradiol (17-βE)-loaded, CREKA-peptide-modified nanoemulsion system on experimental atherosclerosis. In vitro treatment of cultured human aortic endothelial cells (ECs) with the 17-βE-loaded, CREKA-peptide-modified nanoemulsion system increased cellular nitrate/nitrite, indicating improved nitric oxide formation. In vivo, systemic administration of this nanoemulsion system to apolipoprotein-E knock out (ApoE-/-) mice fed a high-fat diet significantly improved multiple parameters related to the etiology and development of occlusive atherosclerotic vasculopathy: lesion area, circulating plasma lipid levels, and expression of aortic-wall inflammatory markers. These salutary effects were attributed selectively to the 17-βE and/or ω-3 polyunsaturated fatty acid components of the nano-delivery system. At therapeutic doses, the 17-βE-loaded, CREKA-peptide modified nanoemulsion system appeared to be biocompatible in that it elicited no apparent adverse/toxic effects, as indexed by body weight, plasma alanine aminotransferase/aspartate aminotransferase levels, and liver and kidney histopathology. The study demonstrates the therapeutic potential of a novel, 17-βE-loaded, CREKA-peptide-modified nanoemulsion system against atherosclerosis in a multimodal fashion by reducing lesion size, lowering the levels of circulating plasma lipids and decreasing the gene expression of inflammatory markers associated with the disease. 相似文献
2.
Mansoor N. Saleh Albert F. LoBuglio Richard H. Wheeler Kimberly J. Rogers Amy Haynes Jeannette Y. Lee M. B. Khazaeli 《Cancer immunology, immunotherapy : CII》1990,32(3):185-190
Summary A group of 15 patients with metastatic colorectal adenocarcinoma received a combination of interferon (0.1 mg/m2, days 1–15) and the murine monoclonal antibody 17-1A (400 mg, days 5, 7, 9 and 12). The treatment was tolerated with minimal toxicity. Of the 14 evaluable patients, 13 developed human antibody to murine 17-1A, with 11 patients demonstrating antibody to the variable region of 17-1A (anti-idiotype). Antibody to the variable region was inhibited by 17-1A but not by mouse immunoglobulin. Sera from patients with substantial anti-idiotype reactivity were capable of inhibiting the binding of murine 17-1A to antigen expressing LS174-T cells thus indicating the presence of antibody directed against the 17-1A combining site (mirror-image anti-idiotype). The median survival of the whole group was 56 weeks and there was no correlation between clinical response/survival and the development of anti-idiotype antibody.Supported by the Veterans Administration Medical Center and by Public Health Services grant CA 45 232 from the National Cancer Institute, National Institutes of Health, Department of Health and Human Services 相似文献
3.
Regional localisations and linkage relationships of seven RFLPs and myotonic dystrophy on chromosome 19 总被引:4,自引:2,他引:2
D. J. Shaw A. L. Meredith M. Sarfarazi H. G. Harley S. M. Huson J. D. Brook L. Bufton M. Litt T. Mohandas P. S. Harper 《Human genetics》1986,74(3):262-266
Summary We have studied the genetic linkage relationships of seven DNA polymorphisms on chromosome 19, with each other and with the myotonic dystrophy locus. The DNA sequences were localised to various regions of the chromosome using translocations in somatic cell hybrids. These results provide the basis for a linkage map of most of chromosome 19, and suggest that the myotonic dystrophy locus is close to the centromere. 相似文献
4.
Sandra Youngman Mansoor Sarfarazi Maja Bucan Marcy MacDonald Barbara Smith Michael Zimmer Conrad Gilliam Anna-Maria Frischauf John J. Wasmuth James F. Gusella Hans Lehrach Peter S. Harper Duncan J. Shaw 《Genomics》1989,5(4)
Genetic linkage studies have mapped Huntington's disease (HD) to the distal portion of the short arm of chromosome 4 (4p16.3), 4 cM distal to D4S10 (G8). To date, no definite flanking marker has been identified. A new DNA marker, D4S90 (D5), which maps to the distal region of 4p16.3, is described. The marker was used in a genetic linkage study in the CEPH reference families with seven other markers at 4p16. The study, together with knowledge of the physical map of the region, places D4S90 as the most distal marker, 6 cM from D4S10. A provisional linkage study with HD gave a maximum lod score of 2.14 at a θ of 0.00 and no evidence of linkage disequilibrium. As D4S90 appears to be located terminally, it should play an important role in the accurate mapping and cloning of the HD gene. 相似文献
5.
6.
Myotonia congenita (Thomsen's disease) excluded from the region of the myotonic dystrophy locus on chromosome 19 总被引:1,自引:0,他引:1
Manuela Koch Helen Harley M. Sarfarazi K. Bender T. Wienker Barbara Zoll P. S. Harper 《Human genetics》1989,82(2):163-166
Summary Linkage analysis has been carried out in six German families with autosomal dominantly inherited myotonia congenita (Thomsen's disease) using five chromosome 19 markers known to be linked to the gene for myotonic dystrophy (DM). Two of the markers, APOC1 and APOC2, are tightly linked to DM. Close linkage between these markers and myotonia congenita (MC) has been excluded to a distance of 9cM (z=-2.158). These data support the clinical suggestion that MC and DM are non-allelic disorders. 相似文献
7.
8.
Iqra Farooq Z.A. Qadri Z.A. Rather Imtiyaz T. Nazki Neelofar Banday Sadaf Rafiq Khalid Z. Masoodi Ahmed Noureldeen Sheikh Mansoor 《Saudi Journal of Biological Sciences》2021,28(7):3701-3709
An efficient protocol for in-vitro propagation of an important ornamental crop, Petunia hybrida Vilm. Cv. “Bravo” was developed. The explants that were used to carry out the experiment were Leaf segments, nodal segments and shoot tips. Nodal segments recorded highest per cent asepsis followed by shoot tips and leaf segments. Asepsis was found to be highest when the explants were sterilized with Fungicide (Carbendazim) 0.02% for the duration of 30 min followed by 0.1% HgCl2 for duration of 10 min and then ethanol 70% for 10 s. Longer duration of the sterilant treatment showed more necrotic effects on the explants, thus mercuric chloride treatment when given for 5 min proved to be more effective in terms of survival of the explants. Maximum establishment per cent was recorded in Murashige and Skoog (MS) media fortified with BAP (1.5 mg L−1) and IBA (0.5 mg L−1) in shoot tips and nodal segments, i.e. 97.90 and 95.74% respectively. Callus was efficiently induced and developed when PGR amalgamation of BAP (0.1 mg L−1) and 2,4-D (1.5mg L−1) was used. Kinetin at the concentration of 2.0 mg L−1 along with IBA at 0.5mg L−1 recorded highest callus regeneration in both leaf and internodal segment derived callus. Maximum proliferation percent of shoots (97.90%), highest number of shoots (20.50 explant−1) and maximum length of shoot (2.70 cm) was recorded in PGR combination of IBA and BAP both at 0.5 mg L−1 concentration level. Rhizogenesis was recorded to be highest in the MS media containing IBA 1.00 mg L−1. Best hardening media which recorded maximum survival per cent 92.50% was noticed on the media formulation comprised of equal ratio of perlite and vermiculite mix, under poly house conditions. 相似文献
9.
Faheem Ahmad M. A. Rather Mansoor A. Siddiqui 《Archives Of Phytopathology And Plant Protection》2013,46(2):168-173
Glasshouse experiment was conducted to assess the impact of green chopped leaves of four test plants (Lantana camara, Ficus virens, Kigelia pinnata and Ficus bengalensis) and two nematicides (Phorate and Carbofuran) on the plant growth parameters of tomato cv. K25 and on the root-knot development. Results revealed that all the tested treatments significantly (p = 0.05) improved plant growth parameters and reduced root-knot development compared to control. Among the tested organic additives, chopped green leaves of Lantana camara added to soil gave the highest enhancement in plant growth parameters, including plant height, fresh and dry weight, number of fruits and fruit weight with the values of 94.2 cm, 106.8 g, 31.6 g, 7.2 and 153.3 g respectively, as well as a greater reduction of Meloidogyne javanica reproduction and development but exhibiting a lower response compared to nematicides. There was also significant reduction in root-knot development in tomato plants growing in other organic additive amended soil. 相似文献
10.
Density-dependent responses of Picea purpurea seedlings for plant growth and resource allocation under elevated temperature 总被引:1,自引:0,他引:1
Jinchuang Wang Baoli Duan Yuanbin Zhang Mansoor Ahmed Bughio Wei Jia 《Trees - Structure and Function》2013,27(6):1775-1787
This study was conducted to determine whether plants in the presence or absence of competition differ in their responses to warming, and whether density modifies the effect of warming. Picea purourea seedlings were grown under ambient and warming (ambient +2.2 °C) conditions in climate control chambers with two different planting densities. After 4 years, seedlings were harvested and measured for height, stem diameter, leaf area, structural biomass, carbon, nitrogen, chlorophyll and carbohydrate levels of needles, branches, stem and roots. At low density, warming increased height, stem diameter, total leaf area biomass production and carbohydrate concentration per seedling, while it decreased C/N ratio for all plant parts, but did not affect chlorophyll content. By contrast, at high density, although warming increased biomass and total leaf area, it did not affect plant height and stem diameter. At the same time, it had different effects on chlorophyll content, C/N ratio and carbohydrate levels among plant parts. On the other hand, high density limited plant growth and altered resource allocation pattern. Our study demonstrates that planting densities decreased the temperature-induced growth enhancement of P. purpurea seedlings and the effects of warming on resource allocation not only showed density-dependence, but also vary with tissue age classes and root diameter; the responses of plants to elevated temperature, acquired from plants growing as individuals, may not be applicable to plants grown under intraspecific competition as typically found in the field. 相似文献