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111.
The biodegradation of Congored, a toxic azo dye, was studied by using a hybrid technique involving sonolysis as pretreatment followed by biological treatment. The experiments were carried out with and without pretreatment using dye solution as a sole source of nutrition with an isolated and acclimatized strain of Bacillus sp. obtained from tannery industry effluent. The pretreatment time was varied as 30, 60, 90, 120, 150 and 180 min and then the pretreated dye solution was subjected to biological treatment. The effectiveness of pretreatment was compared with the results of biological degradation of non pretreated Congored and the results showed that the pretreatment improved the efficiency of the biodegradation of Congored. During the biological degradation, the increase in initial dye concentration decreased the decolorization rate and at high concentrations (1500 and 2000 mg/l), the inhibition was observed. The optimum pH and temperature were determined to be 7.0 and 37 degrees C, respectively. The data obtained through biodegradation experiments were fitted with five different kinetic models and the results were analyzed. 相似文献
112.
113.
Hassanain Al-Talib Chan Yean Yean Alyaa Al-Khateeb Habsah Hassan Kirnpal-Kaur Banga Singh Karim Al-Jashamy Manickam Ravichandran 《BMC microbiology》2009,9(1):113-8
Background
Staphylococcus aureus is a major human pathogen, especially methicillin-resistant S. aureus (MRSA), which causes a wide range of hospital and community-acquired infections worldwide. Conventional testing for detection of MRSA takes 2–5 days to yield complete information of the organism and its antibiotic sensitivity pattern. 相似文献114.
Manickam Sugumaran Brian Hennigan Jean O'Brien 《Archives of insect biochemistry and physiology》1987,6(1):9-25
The validity of Pryor's widely accepted quinone tanning hypothesis for the sclerotization of insect cuticle was examined using an in vitro model system. Quinones generated in situ by the oxidation of catechols with mushroom tyrosinase and molecular oxygen readily reacted with test proteins such as lysozyme, ribonuclease and cytochrome-c, producing dimers, trimers, and higher oligomers. With the exception of 3,4-dihydroxyphenylalanine, dopamine, and norepinephrine, most other catechols tested participated in protein polymerization. The inability of these three compounds to support oligomerization of test protein was attributed to their high rate of intramolecular cyclization reaction. Radioactive incorporation studies reveal the formation of catechol-protomer adducts, as well as aryl-protein crosslinks in the reaction mixture. The above results strongly support the quinone tanning hypothesis. Based on these findings, the mechanism of cuticular sclerotization is discussed. 相似文献
115.
116.
The poly(A)-rich RNA from dry mung bean (Vigna radiata [L.] Wilczek) embryonic axes has been isolated and translated in a wheat embryo cell-free system, and the products were analyzed on sodium dodecyl sulfate-polyacrylamide gels. The fluorographyic patterns showed a heavy band at approximately MW 12,000. The messenger RNA coding for this polypeptide disappeared in the course of early germination. This messenger is translated in vivo but simultaneously degrades when the axes imbibe. The poly(A)-rich RNA from dry axes has been fractionated on sucrose-dimethyl sulfoxide gradients, and this messenger has been found to be distributed largely in the 9–14 S region. The polypeptide synthesized in vitro has been immunoprecipitated, using the antiserum raised against this protein purified from dry axes.Abbreviations DMSO
dimethyl sulfoxide
- DTT
dithiothreitol
- EDTA
ethylene diamine tetraacetic acid
- HEPES
N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid
- SHB
standard HEPES buffer
- SDS-PAGE
sodium dodecyl sulfate-polyacryl-amide gel electrophoresis
- TMV-RNA
tobacco mosaic virus RNA 相似文献
117.
118.
A 2.8-Mb Clone Contig of the Multiple Endocrine Neoplasia Type 1 (MEN1) Region at 11q13 总被引:1,自引:0,他引:1
Siradanahalli C. Guru Shodimu-Emmanuel Olufemi Pachiappan Manickam Christiano Cummings Linn M. Gieser Brian L. Pike Michael L. Bittner Yuan Jiang A.Craig Chinault Norma J. Nowak Anna Brzozowska Judy S. Crabtree Yingping Wang Bruce A. Roe Jane M. Weisemann Mark S. Boguski Sunita K. Agarwal A.Lee Burns Allen M. Spiegel Stephen J. Marx Wendy L. Flejter Pieter J. de Jong Francis S. Collins Settara C. Chandrasekharappa 《Genomics》1997,42(3):436
119.
Incubation of N-acetyltyrosine methyl ester with cuticular enzymes, isolated from the wandering stages of Calliphora sp larvae, resulted in the generation of N-acetyldopa methyl ester when the reaction was carried out in the presence of ascorbate which prevented further oxidation of the o-diphenolic product. Enzymatic oxidation of N-acetyldopa methyl ester ultimately generated dehydro N-acetyldopa methyl ester. The identity of enzymatically produced N-acetyldopa methyl ester and dehydro N-acetyldopa methyl ester has been confirmed by comparison of the ultraviolet and infrared spectral and chromatographic properties with those of authentic samples as well as by nuclear magnetic resonance studies. Since N-acetyldopaquinone methyl ester was also converted to dehydro N-acetyldopa methyl ester and tyrosinase was responsible for the oxidation of N-acetyldopa methyl ester, a scheme for the cuticular phenoloxidase catalyzed conversion of N-acetyltyrosine methyl ester to dehydro N-acetyldopa methyl ester involving the intermediary formation of the quinone and the quinone methide is proposed to account for the observed results. The conversion of N-acetyldopa methyl ester to dehydro derivative remarkably resembles the conversion of the sclerotizing precursor, N-acetyldopamine, to dehydro-N-acetyl-dopamine observed in the insect cuticle. Based on these comparative studies, it is proposed that peptidyl dopa derivatives could also serve as the sclerotizing precursors for the sclerotization of the insect cuticle. © 1995 Wiley-Liss, Inc. 相似文献
120.
Modulatory Effect of Taurine on 7,12‐Dimethylbenz(a)Anthracene‐Induced Alterations in Detoxification Enzyme System,Membrane Bound Enzymes,Glycoprotein Profile and Proliferative Cell Nuclear Antigen in Rat Breast Tissue 下载免费PDF全文
Manickam Kalappan Vanitha Kuppusamy Baskaran Kuppusamy Periyasamy Sundaramoorthy Selvaraj Aruldoss Ilakkia Dhiravidamani Saravanan Ramachandran Venkateswari Balasundaram Revathi Mani Pandi Anandakumar Dhanapal Sakthisekaran 《Journal of biochemical and molecular toxicology》2016,30(8):414-423
The modulatory effect of taurine on 7,12‐dimethylbenz(a)anthracene (DMBA)‐induced breast cancer in rats was studied. DMBA (25 mg/kg body weight) was administered to induce breast cancer in rats. Protein carbonyl levels, activities of membrane bound enzymes (Na+/K+ATPase, Ca2+ATPase, and Mg2+ATPase), phase I drug metabolizing enzymes (cytochrome P450, cytochrome b5, NADPH cytochrome c reductase), phase II drug metabolizing enzymes (glutathione‐S‐transferase and UDP‐glucuronyl transferase), glycoprotein levels, and proliferative cell nuclear antigen (PCNA) were studied. DMBA‐induced breast tumor bearing rats showed abnormal alterations in the levels of protein carbonyls, activities of membrane bound enzymes, drug metabolizing enzymes, glycoprotein levels, and PCNA protein expression levels. Taurine treatment (100 mg/kg body weight) appreciably counteracted all the above changes induced by DMBA. Histological examination of breast tissue further supported our biochemical findings. The results of the present study clearly demonstrated the chemotherapeutic effect of taurine in DMBA‐induced breast cancer. 相似文献