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111.
Manfredi P Renzi F Mally M Sauteur L Schmaler M Moes S Jenö P Cornelis GR 《Molecular microbiology》2011,81(4):1050-1060
Capnocytophaga canimorsus are commensal Gram-negative bacteria from dog's mouth that cause rare but dramatic septicaemia in humans. C. canimorsus have the unusual property to feed on cultured mammalian cells, including phagocytes, by harvesting the glycan moiety of cellular glycoproteins. To understand the mechanism behind this unusual property, the genome of strain Cc5 was sequenced and analysed. In addition, Cc5 bacteria were cultivated onto HEK 293 cells and the surface proteome was determined. The genome was found to encode many lipoproteins encoded within 13 polysaccharide utilization loci (PULs) typical of the Flavobacteria-Bacteroides group. PULs encode surface exposed feeding complexes resembling the archetypal starch utilization system (Sus). The products of at least nine PULs were detected among the surface proteome and eight of them represented more than half of the total peptides detected from the surface proteome. Systematic deletions of the 13 PULs revealed that half of these Sus-like complexes contributed to growth on animal cells. The complex encoded by PUL5, one of the most abundant ones, was involved in foraging glycans from glycoproteins. It was essential for growth on cells and contributed to survival in mice. It thus represents a fitness factor during infection. 相似文献
112.
Mark P. Culik José Aires Ventura Lúcia Massutti de Almeida Geovan Henrique Corrêa 《Biocontrol Science and Technology》2011,21(2):235-238
Feeding by the coccinellid Psyllobora rufosignata on the rust fungus Phakopsora euvitis was confirmed by observing uredospores of P. euvitis in gut contents of Ps. rufosignata. This is the first record of Ps. rufosignata feeding on P. euvitis and the first record of any Halyziini species feeding on any species of Basidiomycota. 相似文献
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Foo LC Allen NJ Bushong EA Ventura PB Chung WS Zhou L Cahoy JD Daneman R Zong H Ellisman MH Barres BA 《Neuron》2011,71(5):799-811
The inability to purify and culture astrocytes has long?hindered studies of their function. Whereas astrocyte progenitor cells can be cultured from neonatal brain, culture of mature astrocytes from postnatal brain has not been possible. Here, we report a new method to prospectively purify astrocytes by immunopanning. These astrocytes undergo apoptosis in culture, but vascular cells and HBEGF promote their survival in serum-free culture. We found that some developing astrocytes normally undergo apoptosis in?vivo and that the vast majority of astrocytes contact blood vessels, suggesting that?astrocytes are matched to blood vessels by competing for vascular-derived trophic factors such as HBEGF. Compared to traditional astrocyte cultures, the gene profiles of the cultured purified postnatal astrocytes much more closely resemble those of in?vivo astrocytes. Although these astrocytes strongly promote synapse formation and function, they do not secrete glutamate in response to stimulation. 相似文献
114.
Initial analysis of copy number variations in cattle selected for resistance or susceptibility to intestinal nematodes 总被引:1,自引:0,他引:1
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Widespread regulatory activity of vertebrate microRNA* species 总被引:1,自引:0,他引:1
Yang JS Phillips MD Betel D Mu P Ventura A Siepel AC Chen KC Lai EC 《RNA (New York, N.Y.)》2011,17(2):312-326
118.
A canonical correlation analysis of the association between carcass and ham traits in pigs used to produce dry-cured ham 总被引:1,自引:0,他引:1
Ventura HT Lopes PS Peloso JV Guimarães SE Carneiro AP Carneiro PL 《Genetics and molecular biology》2011,34(3):451-455
The association between carcass and ham traits in a pig population used to produce dry-cured ham was studied using canonical correlation analysis. The carcass traits examined were hot carcass weight (HCW), backfat thickness (BT) and loin depth (LD), and the ham traits studied were gross ham weight (GHW), trimmed ham weight (THW), ham inner layer fat thickness (HIFT), ham outer layer fat thickness (HOFT), pH (pH) and the Göfo value. Carcass and ham traits are not independent. The canonical correlations (r) between the carcass and ham traits at 130 kg were 0.77, 0.24 and 0.20 for the first, second and third canonical pair, respectively, and were all significant (p < 0.01) by the Wilks test. The corresponding canonical correlations between the three canonical variate pairs for the carcass and ham traits at 160 kg were 0.88, 0.42 and 0.14, respectively (p < 0.05 for all, except the third). The correlations between the traits and their canonical variate showed an association among HCW, GHW and THW, and between BT and HOFT. These results indicate that carcass traits should be used to cull pigs that are not suitable for dry-cured ham production. 相似文献
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