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991.
992.
Matthias May Sabine Brookman-May Bernd Hoschke Christian Gilfrich Friederike Kendel Susann Baxmann Stefan Wittke Stephan T. Kiessig Kurt Miller Manfred Johannsen 《Cancer immunology, immunotherapy : CII》2010,59(5):687-695
About 30% of renal cell carcinomas (RCC) will develop recurrence after surgery. Despite evidence for a significantly improved
survival by autologous tumour cell vaccination therapy, the procedure has not become standard. Between August 1993 and December
1996, 1,267 RCC patients undergoing radical nephrectomy in 84 German hospitals were subsequently treated by autologous tumour
cell vaccination therapy. The study group comprised 692 patients with complete follow-up (stages pT2-3, pNx-2, M0 based on
the TNM classification, 4th edition). Subsequent propensity-score matching according to 7 defined criteria with 861 control
patients undergoing nephrectomy alone without adjuvant treatment at the Carl-Thiem-Hospital Cottbus, resulted in 495 matched
pairs. Overall and stage-specific survival rates were analysed after a median follow-up of 131 months. The 5- and 10-year
overall survival (OS) rates were 80.6 and 68.9% in the vaccine group and 79.2 and 62.1% in the control group (p = 0.066). Patients with pT3 stage RCC revealed 5- and 10-year OS rates of 71.3 and 53.6% in the study group and 65.4 and
36.2% in the control group (p = 0.022). In multivariable analysis, patients in the vaccine group showed a significantly improved survival both in the whole
study group (HR = 1.28, p = 0.030) and in the subgroup presenting with pT3 stage tumours (HR = 1.67, p = 0.011). Adjuvant treatment with autologous vaccination therapy resulted in a significantly improved overall survival in
pT3 stage RCC patients, suggesting benefit especially in this subgroup. However, controlled clinical trials integrating the
recent TNM classification and further risk constellations are required to define additional patient groups that may derive
benefit from this treatment. 相似文献
993.
Manuela Nowotny Jennifer Hummel Melanie Weber Doreen Möckel Manfred Kössl 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2010,196(12):939-945
Bushcrickets have a tonotopically organised hearing organ, the so-called crista acustica, in the tibia of the forelegs. This organ responds to a frequency range of about 5–80 kHz and lies behind the anterior tympanum
on top of a trachea branch. We analyzed the sound-induced vibration pattern of the anterior tympanum, using a Laser-Doppler-Vibrometer
Scanning microscope system, in order to identify frequency-dependent amplitude and phase of displacement. The vibration pattern
evoked by a frequency sweep (4–79 kHz) showed an amplitude maximum which would correspond to the resonance frequency of an
open tube system. At higher frequencies of about 30 kHz a difference in the amplitude and phase response between the distal
and the proximal part of the tympanum was detected. The inner plate of the tympanum starts to wobble at this frequency. This
higher mode in the motion pattern is not explained by purely acoustic characteristics of the tracheal space below the tympanum
but may depend on the mechanical impedance of the tympanum plate. In accordance with a previous hypothesis, the tympanum moves
over the whole tested frequency range in the dorso-ventral direction like a hinged flap with the largest displacement in its
ventral part and no higher modes of vibration. 相似文献
994.
995.
Cornelia Hunke Vikeramjeet Singh Tadwal Malathy Sony Subramanian Manimekalai Manfred Roessle Gerhard Grüber 《Journal of bioenergetics and biomembranes》2010,42(1):1-10
Subunit α of the Escherichia coli F1FO ATP synthase has been produced, and its low-resolution structure has been determined. The monodispersity of α allowed the studies of nucleotide-binding and inhibitory effect of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) to ATP/ADP-binding. Binding constants (K d ) of 1.6 μM of bound MgATP-ATTO-647N and 2.9 μM of MgADP-ATTO-647N have been determined from fluorescence correlation spectroscopy data. A concentration of 51 μM and 55 μM of NBD-Cl dropped the MgATP-ATTO-647N and MgADP-ATTO-647N binding capacity to 50% (IC50), respectively. In contrast, no effect was observed in the presence of N,N′-dicyclohexylcarbodiimide. As subunit α is the homologue of subunit B of the A1AO ATP synthase, the interaction of NBD-Cl with B of the A-ATP synthase from Methanosarcina mazei Gö1 has also been shown. The data reveal a reduction of nucleotide-binding of B due to NBD-Cl, resulting in IC50 values of 41 μM and 42 μM for MgATP-ATTO-647N and MgADP-ATTO-647N, respectively. 相似文献
996.
Christine B. Schmitt Feyera Senbeta Manfred Denich Helmut Preisinger Hans Juergen Boehmer 《African Journal of Ecology》2010,48(1):78-86
Coffea arabica occurs naturally in the montane rainforests of Ethiopia, but large areas of these unique forests have been converted to other land-uses. In the remaining forest, wild coffee is managed and harvested with increasing intensity because of rising coffee prices in the world market. This study evaluated the impact of coffee management on wild coffee populations and the forest vegetation as a basis for conservation planning in southwestern Ethiopia. Vegetation surveys and yield assessments were carried out in unmanaged natural forest and in managed semi-forest coffee (SFC) systems. Analyses show that wild coffee density and coffee yields were low in natural forest (max. 15 kg ha−1 year−1 ). In SFC systems, 30% of the canopy trees and most undergrowth vegetation were removed. This stimulated wild coffee growth and strongly enhanced yields (max. 54 kg ha−1 year−1 ), but severely disturbed forest structure. Species richness increased by 26% because of an increase in species of ruderal and secondary vegetation; however, species richness and abundance of typical forest species declined. Conservation of the natural forest therefore requires the control of wild coffee management. Wild coffee certification is discussed as one tool to reconcile conservation measures and the interests of local farmers. 相似文献
997.
Clemens Röhrl Stefanie Fruhwürth Sabine Maria Schreier Alfred Lohninger Andrea Dolischka Manfred Hüttinger Nina Zemann Marcela Hermann Witta Strobl Herbert Stangl 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2010,1801(2):198-204
Recent evidence suggests that scavenger receptor, class B, type I (SR-BI) plays a physiological role in VLDL metabolism. SR-BI was reported to mediate β-VLDL uptake; however, cellular details of this process are not well characterized. In the present study we show that SR-BI delivers cholesterol derived from β-VLDL to LDL receptor negative SR-BI over-expressing Chinese Hamster Ovarian cells (ldlA7-SRBI). Cell association of β-VLDL was ∼ 3 times higher after SR-BI over-expression, which was competed by β-VLDL, but only to a lesser extent by HDL and LDL. Almost all of the associated β-VLDL was located intracellularly, and therefore could not be released by a 50-fold excess of unlabeled β-VLDL. β-VLDL was degraded at a rate of 6 ng β-VLDL/mg cell protein and hour. In contrast to ldlA7 cells, β-VLDL association was competed by LDL in cells with a functional LDL receptor like CHO and HepG2 cells, indicating a strong impact of the LDL receptor in β-VLDL uptake. β-VLDL degradation was similar to ldlA7-SRBI cells. When β-VLDL uptake was followed using fluorescence microscopy, β-VLDL showed a different uptake pattern in SR-BI over-expressing cells, ldlA7-SRBI, compared to LDL receptor containing cells, CHO and HepG2. 相似文献
998.
Sohini Ghoshroy Manfred Binder Aurélien Tartar Deborah L Robertson 《BMC evolutionary biology》2010,10(1):198
Background
Glutamine synthetase (GS) is essential for ammonium assimilation and the biosynthesis of glutamine. The three GS gene families (GSI, GSII, and GSIII) are represented in both prokaryotic and eukaryotic organisms. In this study, we examined the evolutionary relationship of GSII from eubacterial and eukaryotic lineages and present robust phylogenetic evidence that GSII was transferred from γ-Proteobacteria (Eubacteria) to the Chloroplastida. 相似文献999.
Julia Garbe Andrea Wesche Boyke Bunk Marlon Kazmierczak Katherina Selezska Christine Rohde Johannes Sikorski Manfred Rohde Dieter Jahn Max Schobert 《BMC microbiology》2010,10(1):301
Background
Pseudomonas aeruginosa causes lung infections in patients suffering from the genetic disorder Cystic Fibrosis (CF). Once a chronic lung infection is established, P. aeruginosa cannot be eradicated by antibiotic treatment. Phage therapy is an alternative to treat these chronic P. aeruginosa infections. However, little is known about the factors which influence phage infection of P. aeruginosa under infection conditions and suitable broad host range phages. 相似文献1000.
Xin Lu Jibin Sun Manfred Nimtz Josef Wissing An-Ping Zeng Ursula Rinas 《Microbial cell factories》2010,9(1):23